LOCUS       RG223144                9083 bp    DNA     circular  SYN 
DEFINITION  KCNQ2 (tGFP-tagged) - Human potassium voltage-gated channel, KQT-like subfamily, member 2 (KCNQ2), transcript variant 4
ACCESSION   NM_172108
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3564..4268 
                     /label="GFP"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    6992..7637 
                     /label="ColE1"  
     misc_feature    5354..6148 
                     /label="Neo"  
     misc_feature    4985..5287 
                     /label="SV40 ori"  
     misc_feature    7652..8512 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3543..3549 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3543 
                     /label="KCNQ2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCAGAAG TCGCGCAACG GCGGCGTATA CCCCGGCCCG AGCGGGGAGA 
     1081 AGAAGCTGAA GGTGGGCTTC GTGGGGCTGG ACCCCGGCGC GCCCGACTCC ACCCGGGACG 
     1141 GGGCGCTGCT GATCGCCGGC TCCGAGGCCC CCAAGCGCGG CAGCATCCTC AGCAAACCTC 
     1201 GCGCGGGCGG CGCGGGCGCC GGGAAGCCCC CCAAGCGCAA CGCCTTCTAC CGCAAGCTGC 
     1261 AGAATTTCCT CTACAACGTG CTGGAGCGGC CGCGCGGCTG GGCGTTCATC TACCACGCCT 
     1321 ACGTGTTCCT CCTGGTTTTC TCCTGCCTCG TGCTGTCTGT GTTTTCCACC ATCAAGGAGT 
     1381 ATGAGAAGAG CTCGGAGGGG GCCCTCTACA TCCTGGAAAT CGTGACTATC GTGGTGTTTG 
     1441 GCGTGGAGTA CTTCGTGCGG ATCTGGGCCG CAGGCTGCTG CTGCCGGTAC CGTGGCTGGA 
     1501 GGGGGCGGCT CAAGTTTGCC CGGAAACCGT TCTGTGTGAT TGACATCATG GTGCTCATCG 
     1561 CCTCCATTGC GGTGCTGGCC GCCGGCTCCC AGGGCAACGT CTTTGCCACA TCTGCGCTCC 
     1621 GGAGCCTGCG CTTCCTGCAG ATTCTGCGGA TGATCCGCAT GGACCGGCGG GGAGGCACCT 
     1681 GGAAGCTGCT GGGCTCTGTG GTCTATGCCC ACAGCAAGGA GCTGGTCACT GCCTGGTACA 
     1741 TCGGCTTCCT TTGTCTCATC CTGGCCTCGT TCCTGGTGTA CTTGGCAGAG AAGGGGGAGA 
     1801 ACGACCACTT TGACACCTAC GCGGATGCAC TCTGGTGGGG CCTGATCACG CTGACCACCA 
     1861 TTGGCTACGG GGACAAGTAC CCCCAGACCT GGAACGGCAG GCTCCTTGCG GCAACCTTCA 
     1921 CCCTCATCGG TGTCTCCTTC TTCGCGCTGC CTGCAGGCAT CTTGGGGTCT GGGTTTGCCC 
     1981 TGAAGGTTCA GGAGCAGCAC AGGCAGAAGC ACTTTGAGAA GAGGCGGAAC CCGGCAGCAG 
     2041 GCCTGATCCA GTCGGCCTGG AGATTCTACG CCACCAACCT CTCGCGCACA GACCTGCACT 
     2101 CCACGTGGCA GTACTACGAG CGAACGGTCA CCGTGCCCAT GTACAGTTCG CAAACTCAAA 
     2161 CCTACGGGGC CTCCAGACTT ATCCCCCCGC TGAACCAGCT GGAGCTGCTG AGGAACCTCA 
     2221 AGAGTAAATC TGGACTCGCT TTCAGGAAGG ACCCCCCGCC GGAGCCGTCT CCAAGCCCCC 
     2281 GAGGCGTGGC TGCCAAGGGG AAGGGGTCCC CGCAGGCCCA GACTGTGAGG CGGTCACCCA 
     2341 GCGCCGACCA GAGCCTCGAG GACAGCCCCA GCAAGGTGCC CAAGAGCTGG AGCTTCGGGG 
     2401 ACCGCAGCCG GGCACGCCAG GCTTTCCGCA TCAAGGGTGC CGCGTCACGG CAGAACTCAG 
     2461 AAGCAAGCCT CCCCGGAGAG GACATTGTGG ATGACAAGAG CTGCCCCTGC GAGTTTGTGA 
     2521 CCGAGGACCT GACCCCGGGC CTCAAAGTCA GCATCAGAGC CGTGTGTGTC ATGCGGTTCC 
     2581 TGGTGTCCAA GCGGAAGTTC AAGGAGAGCC TGCGGCCCTA CGACGTGATG GACGTCATCG 
     2641 AGCAGTACTC AGCCGGCCAC CTGGACATGC TGTCCCGAAT TAAGAGCCTG CAGTCCAGAG 
     2701 TGGACCAGAT CGTGGGGCGG GGCCCAGCGA TCACGGACAA GGACCGCACC AAGGGCCCGG 
     2761 CCGAGGCGGA GCTGCCCGAG GACCCCAGCA TGATGGGACG GCTCGGGAAG GTGGAGAAGC 
     2821 AGGTCTTGTC CATGGAGAAG AAGCTGGACT TCCTGGTGAA TATCTACATG CAGCGGATGG 
     2881 GCATCCCCCC GACAGAGACC GAGGCCTACT TTGGGGCCAA AGAGCCGGAG CCGGCGCCGC 
     2941 CGTACCACAG CCCGGAAGAC AGCCGGGAGC ATGTCGACAG GCACGGCTGC ATTGTCAAGA 
     3001 TCGTGCGCTC CAGCAGCTCC ACGGGCCAGA AGAACTTCTC GGCGCCCCCG GCCGCGCCCC 
     3061 CTGTCCAGTG TCCGCCCTCC ACCTCCTGGC AGCCACAGAG CCACCCGCGC CAGGGCCACG 
     3121 GCACCTCCCC CGTGGGGGAC CACGGCTCCC TGGTGCGCAT CCCGCCGCCG CCTGCCCACG 
     3181 AGCGGTCGCT GTCCGCCTAC GGCGGGGGCA ACCGCGCCAG CATGGAGTTC CTGCGGCAGG 
     3241 AGGACACCCC GGGCTGCAGG CCCCCCGAGG GGAACCTGCG GGACAGCGAC ACGTCCATCT 
     3301 CCATCCCGTC CGTGGACCAC GAGGAGCTGG AGCGTTCCTT CAGCGGCTTC AGCATCTCCC 
     3361 AGTCCAAGGA GAACCTGGAT GCTCTCAACA GCTGCTACGC GGCCGTGGCG CCTTGTGCCA 
     3421 AAGTCAGGCC CTACATTGCG GAGGGAGAGT CAGACACCGA CTCCGACCTC TGTACCCCGT 
     3481 GCGGGCCCCC GCCACGCTCG GCCACCGGCG AGGGTCCCTT TGGTGACGTG GGCTGGGCCG 
     3541 GGCCCAGGAA GACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3601 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3661 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3721 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     3781 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     3841 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     3901 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     3961 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4021 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4081 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4141 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4201 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4261 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4321 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4381 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4441 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4501 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4561 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4621 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4681 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4741 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4801 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4861 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4921 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     4981 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5041 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5101 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5161 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5221 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5281 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5341 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5401 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5461 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5521 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5581 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5641 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5701 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     5761 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     5821 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     5881 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     5941 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6001 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6061 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6121 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6181 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6241 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6301 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6361 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6421 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6481 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6541 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6601 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6661 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6721 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     6781 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     6841 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     6901 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     6961 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7021 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7081 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7141 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7201 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7261 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7321 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7381 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7441 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7501 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7561 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7621 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7681 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7741 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     7801 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     7861 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     7921 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     7981 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8041 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8101 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8161 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8221 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8281 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8341 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8401 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8461 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8521 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8581 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8641 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8701 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     8761 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     8821 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     8881 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     8941 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9001 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9061 AACAAAAATT TAACGCGAAT TTT
//