LOCUS       RG222999                8669 bp    DNA     circular  SYN 
DEFINITION  HPS4 (tGFP-tagged) - Human Hermansky-Pudlak syndrome 4 (HPS4), transcript variant 2
ACCESSION   NM_152841
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3150..3854 
                     /label="GFP"  
     misc_feature    3901..4486 
                     /label="PolyA signal"  
     misc_feature    6578..7223 
                     /label="ColE1"  
     misc_feature    4940..5734 
                     /label="Neo"  
     misc_feature    4571..4873 
                     /label="SV40 ori"  
     misc_feature    7238..8098 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3129..3135 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3129 
                     /label="HPS4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCCACTC TGTTCTCTTG CCAGGTGGAA TTATTTTTTT CTTTATGATG 
     1081 GTTCCAAGGT AAAGGAAGAA GGCGATCCAA CAAGAGCTGG CATTTGTTAC TTTTATCCTT 
     1141 CCCAGACCCT GCTAGACCAA CAGGAGTTGC TTTGTGGACA GATTGCTGGA GTTGTCCGCT 
     1201 GTGTTTCTGA CATTTCTGAC TCTCCTCCTA CTCTTGTTCG TCTGAGAAAA CTGAAGTTTG 
     1261 CCATAAAAGT TGATGGAGAT TACCTTTGGG TGCTGGGCTG TGCTGTGGAG CTCCCTGATG 
     1321 TCAGCTGCAA GCGGTTTCTG GATCAGCTAG TTGGATTCTT TAATTTTTAC AATGGACCTG 
     1381 TTTCCCTAGC TTATGAGAAC TGTTCTCAGG AAGAACTGAG CACGGAGTGG GACACCTTCA 
     1441 TCGAGCAAAT TCTGAAAAAC ACCAGTGATC TGCATAAGAT TTTCAATTCC CTCTGGAACT 
     1501 TGGACCAAAC TAAAGTGGAG CCCCTGTTGT TGCTGAAGGC AGCCCGCATT CTGCAGACCT 
     1561 GCCAGCGCTC GCCTCACATT CTCGCTGGCT GCATCCTCTA TAAAGGACTG ATTGTCAGCA 
     1621 CCCAACTCCC GCCCTCCCTC ACCGCCAAGG TCCTGCTTCA CCGAACAGCA CCTCAGGAGC 
     1681 AGAGACTCCC TACGGGAGAG GATGCCCCGC AGGAACATGG AGCGGCATTG CCCCCGAATG 
     1741 TCCAGATTAT CCCTGTTTTT GTGACCAAAG AGGAAGCCAT TAGTCTCCAC GAGTTCCCGG 
     1801 TGGAACAGAT GACAAGGTCT CTAGCATCTC CAGCAGGACT CCAGGATGGT TCAGCCCAGC 
     1861 ACCATCCAAA GGGTGGGAGC ACATCTGCCC TGAAAGAAAA CGCCACTGGC CATGTGGAAT 
     1921 CCATGGCCTG GACCACCCCA GATCCCACAT CCCCTGACGA AGCTTGTCCA GATGGCAGGA 
     1981 AGGAGAACGG ATGCTTGTCT GGCCATGATC TGGAGAGCAT CAGGCCCGCA GGACTGCACA 
     2041 ACTCTGCCAG GGGTGAGGTT CTTGGCCTCA GCTCCTCCCT GGGGAAGGAA CTAGTCTTTC 
     2101 TCCAAGAAGA ACTCGACTTG TCTGAAATCC ACATTCCAGA GGCTCAGGAA GTGGAAATGG 
     2161 CCTCAGGTCA TTTTGCCTTC CTACATGTGC CTGTTCCAGA TGGCAGGGCT CCTTACTGCA 
     2221 AGGCATCTCT CAGCGCCTCC AGCAGCCTGG AACCCACGCC TCCTGAGGAC ACAGCCATCA 
     2281 GCAGCTTGCG CCCTCCCTCT GCTCCTGAGA TGCTGACCCA GCATGGAGCC CAAGAGCAGC 
     2341 TCGAAGACCA TCCTGGCCAT AGCAGCCAAG CCCCCATTCC CAGAGCAGAC CCTCTCCCCA 
     2401 GAAGGACCCG CAGGCCCTTG TTATTGCCTC GCTTAGATCC AGGACAGAGA GGAAACAAGC 
     2461 TTCCCACGGG GGAACAAGGC CTGGATGAGG ATGTTGATGG GGTCTGTGAA AGCCACGCAG 
     2521 CCCCTGGTCT GGAATGCAGT TCAGGCTCAG CAAACTGTCA GGGTGCTGGC CCCTCTGCAG 
     2581 ATGGAATCAG CTCCAGGCTG ACACCAGCAG AGTCCTGCAT GGGGCTCGTG AGGATGAATC 
     2641 TCTACACTCA CTGCGTCAAA GGGCTGGTGC TGTCCCTGCT GGCTGAGGAG CCGCTGCTGG 
     2701 GAGACAGCGC AGCCATAGAG GAAGTGTACC ACAGCAGCCT GGCTTCACTG AATGGGCTGG 
     2761 AAGTCCACCT GAAAGAGACG CTGCCCAGGG ATGAGGCAGC CTCCACGAGC AGCACCTACA 
     2821 ACTTCACACA TTACGACCGC ATTCAGAGCT TGCTGATGGC AAACCTGCCG CAGGTGGCCA 
     2881 CCCCGCAGGA TCGCCGCTTC CTCCAGGCCG TCAGCCTGAT GCATAGCGAA TTTGCCCAGC 
     2941 TGCCCGCGCT TTATGAAATG ACTGTCAGAA ATGCCTCCAC GGCTGTGTAC GCCTGTTGCA 
     3001 ACCCCATCCA GGAGACATAT TTCCAGCAGC TGGCACCTGC AGCACGGAGC TCCGGCTTCC 
     3061 CAAACCCTCA GGATGGCGCC TTCAGCCTCT CCGGCAAAGC AAAGCAGAAG CTGCTGAAGC 
     3121 ACGGGGTGAA CTTGCTCACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3181 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3241 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3301 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3361 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3421 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     3481 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     3541 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     3601 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     3661 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     3721 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     3781 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     3841 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3901 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3961 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4021 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4081 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4141 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4201 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4261 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4321 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4381 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4441 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4501 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     4561 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4621 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4681 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     4741 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     4801 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     4861 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     4921 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     4981 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5041 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5101 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5161 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5221 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5281 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5341 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5401 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     5461 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     5521 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     5581 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     5641 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     5701 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     5761 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     5821 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     5881 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     5941 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6001 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6061 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6121 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6181 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6241 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6301 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6361 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6421 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6481 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     6541 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     6601 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     6661 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     6721 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     6781 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     6841 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     6901 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     6961 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7021 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7081 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7141 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7201 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7261 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7321 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7381 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7441 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     7501 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     7561 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     7621 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     7681 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     7741 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     7801 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     7861 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     7921 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     7981 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8041 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8101 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8161 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8221 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8281 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8341 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8401 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     8461 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     8521 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     8581 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     8641 TGATTTAACA AAAATTTAAC GCGAATTTT
//