LOCUS       RG222540                9326 bp    DNA     circular  SYN 
DEFINITION  GRM7 (tGFP-tagged) - Human glutamate receptor, metabotropic 7 (GRM7), transcript variant 2
ACCESSION   NM_181874
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3807..4511 
                     /label="GFP"  
     misc_feature    4558..5143 
                     /label="PolyA signal"  
     misc_feature    7235..7880 
                     /label="ColE1"  
     misc_feature    5597..6391 
                     /label="Neo"  
     misc_feature    5228..5530 
                     /label="SV40 ori"  
     misc_feature    7895..8755 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3786..3792 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3786 
                     /label="GRM7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCAGCTG AGGAAGCTGC TCCGCGTCCT GACTTTGATG AAGTTCCCCT 
     1081 GCTGCGTGCT GGAGGTGCTC CTGTGCGCGC TGGCGGCGGC GGCGCGCGGC CAGGAGATGT 
     1141 ACGCCCCGCA CTCAATCCGG ATCGAGGGGG ACGTCACCCT CGGGGGGCTG TTCCCCGTGC 
     1201 ACGCCAAGGG TCCCAGCGGA GTGCCCTGCG GCGACATCAA GAGGGAAAAC GGGATCCACA 
     1261 GGCTGGAAGC GATGCTCTAC GCCCTGGACC AGATCAACAG TGATCCCAAC CTACTGCCCA 
     1321 ACGTGACGCT GGGCGCGCGG ATCCTGGACA CTTGTTCCAG GGACACTTAC GCGCTCGAAC 
     1381 AGTCGCTTAC TTTCGTCCAG GCGCTCATCC AGAAGGACAC CTCCGACGTG CGCTGCACCA 
     1441 ACGGCGAACC GCCGGTTTTC GTCAAGCCGG AGAAAGTAGT TGGAGTGATT GGGGCTTCGG 
     1501 GGAGTTCGGT CTCCATCATG GTAGCCAACA TCCTGAGGCT CTTCCAGATC CCCCAGATTA 
     1561 GTTATGCATC AACGGCACCC GAGCTAAGTG ATGACCGGCG CTATGACTTC TTCTCTCGCG 
     1621 TGGTGCCACC CGATTCCTTC CAAGCCCAGG CCATGGTAGA CATTGTAAAG GCCCTAGGCT 
     1681 GGAATTATGT GTCTACCCTC GCATCGGAAG GAAGTTATGG AGAGAAAGGT GTGGAGTCCT 
     1741 TCACGCAGAT TTCCAAAGAG GCAGGTGGAC TCTGCATTGC CCAGTCCGTG AGAATCCCCC 
     1801 AGGAACGCAA AGACAGGACC ATTGACTTTG ATAGAATTAT CAAACAGCTC CTGGACACCC 
     1861 CCAACTCCAG GGCCGTCGTG ATTTTTGCCA ACGATGAGGA TATAAAGCAG ATCCTTGCAG 
     1921 CAGCCAAAAG AGCTGACCAA GTTGGCCATT TTCTTTGGGT GGGATCAGAC AGCTGGGGAT 
     1981 CCAAAATAAA CCCACTGCAC CAGCATGAAG ATATCGCAGA AGGGGCCATC ACCATTCAGC 
     2041 CCAAGCGAGC CACGGTGGAA GGGTTTGATG CCTACTTTAC GTCCCGTACA CTTGAAAACA 
     2101 ACAGAAGAAA TGTATGGTTT GCCGAATACT GGGAGGAAAA CTTCAACTGC AAGTTGACGA 
     2161 TTAGTGGGTC AAAAAAAGAA GACACAGATC GCAAATGCAC AGGACAGGAG AGAATTGGAA 
     2221 AAGATTCCAA CTATGAGCAG GAGGGTAAAG TCCAGTTCGT GATTGACGCA GTCTATGCTA 
     2281 TGGCTCACGC CCTTCACCAC ATGAACAAGG ATCTCTGTGC TGACTACCGG GGTGTCTGCC 
     2341 CAGAGATGGA GCAAGCTGGA GGCAAGAAGT TGCTGAAGTA TATACGCAAT GTTAATTTCA 
     2401 ATGGTAGTGC TGGCACTCCA GTGATGTTTA ACAAGAACGG GGATGCACCT GGGCGTTATG 
     2461 ACATCTTTCA GTACCAGACC ACAAACACCA GCAACCCGGG TTACCGTCTG ATCGGGCAGT 
     2521 GGACAGACGA ACTTCAGCTC AATATAGAAG ACATGCAGTG GGGTAAAGGA GTCCGAGAGA 
     2581 TACCCGCCTC AGTGTGCACA CTACCATGTA AGCCAGGACA GAGAAAGAAG ACACAGAAAG 
     2641 GAACTCCTTG CTGTTGGACC TGTGAGCCTT GCGATGGTTA CCAGTACCAG TTTGATGAGA 
     2701 TGACATGCCA GCATTGCCCC TATGACCAGA GGCCCAATGA AAATCGAACC GGATGCCAGG 
     2761 ATATTCCCAT CATCAAACTG GAGTGGCACT CCCCCTGGGC TGTGATTCCT GTCTTCCTGG 
     2821 CAATGTTGGG GATCATTGCC ACCATCTTTG TCATGGCCAC TTTCATCCGC TACAATGACA 
     2881 CGCCCATTGT CCGGGCATCT GGGCGGGAAC TCAGCTATGT TCTTTTGACG GGCATCTTTC 
     2941 TTTGCTACAT CATCACTTTC CTGATGATTG CCAAACCAGA TGTGGCAGTG TGTTCTTTCC 
     3001 GGCGAGTTTT CTTGGGCTTG GGTATGTGCA TCAGTTATGC AGCCCTCTTG ACGAAAACAA 
     3061 ATCGGATTTA TCGCATATTT GAGCAGGGCA AGAAATCAGT AACAGCTCCC AGACTCATAA 
     3121 GCCCAACATC ACAACTGGCA ATCACTTCCA GTTTAATATC AGTTCAGCTT CTAGGGGTGT 
     3181 TCATTTGGTT TGGTGTTGAT CCACCCAACA TCATCATAGA CTATGATGAA CACAAGACAA 
     3241 TGAACCCTGA GCAAGCCAGA GGGGTTCTCA AGTGTGACAT TACAGATCTC CAAATCATTT 
     3301 GCTCCTTGGG ATATAGCATT CTTCTCATGG TCACATGTAC TGTGTATGCC ATCAAGACTC 
     3361 GGGGTGTACC CGAGAATTTT AACGAAGCCA AGCCCATTGG ATTCACTATG TACACGACAT 
     3421 GTATAGTATG GCTTGCCTTC ATTCCAATTT TTTTTGGCAC CGCTCAATCA GCGGAAAAGC 
     3481 TCTACATACA AACTACCACG CTTACAATCT CCATGAACCT AAGTGCATCA GTGGCGCTGG 
     3541 GGATGCTATA CATGCCGAAA GTGTACATCA TCATTTTCCA CCCTGAACTC AATGTCCAGA 
     3601 AACGGAAGCG AAGCTTCAAG GCGGTAGTCA CAGCAGCCAC CATGTCATCG AGGCTGTCAC 
     3661 ACAAACCCAG TGACAGACCC AACGGTGAGG CAAAGACCGA GCTCTGTGAA AACGTAGACC 
     3721 CAAACAACTG TATACCACCA GTAAGAAAGA GTGTACAAAA GTCTGTTACT TGGTACACTA 
     3781 TCCCACCAAC AGTAACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3841 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     3901 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     3961 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     4021 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     4081 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     4141 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4201 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4261 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4321 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4381 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4441 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4501 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5221 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5281 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5341 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5401 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5461 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5521 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5581 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5641 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5701 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5761 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5821 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     5881 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     5941 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     6001 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     6061 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     6121 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6181 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6241 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6301 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6361 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6421 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6481 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6541 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6601 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6661 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6721 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6781 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6841 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     6901 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     6961 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     7021 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     7081 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7141 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7201 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7261 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7321 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7381 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7441 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7501 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7561 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7621 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7681 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7741 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7801 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7861 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     7921 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     7981 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     8041 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     8101 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     8161 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8221 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8281 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8341 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8401 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8461 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8521 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8581 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8641 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8701 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8761 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8821 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     8881 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     8941 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     9001 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     9061 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     9121 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9181 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9241 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9301 TTTAACAAAA ATTTAACGCG AATTTT
//