LOCUS       RG222500                9227 bp    DNA     circular  SYN 
DEFINITION  TPO (tGFP-tagged) - Human thyroid peroxidase (TPO), transcript variant 4
ACCESSION   NM_175721
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3709..4413 
                     /label="GFP"  
     misc_feature    4460..5045 
                     /label="PolyA signal"  
     misc_feature    7137..7782 
                     /label="ColE1"  
     misc_feature    5499..6293 
                     /label="Neo"  
     misc_feature    5130..5432 
                     /label="SV40 ori"  
     misc_feature    7797..8657 
                     /label="Amp"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3696..3704 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3696 
                     /label="TPO"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCATGA GAGCGCTCGC TGTGCTGTCT GTCACGCTGG TTATGGCCTG 
     1081 CACAGAAGCC TTCTTCCCCT TCATCTCGAG AGGGAAAGAA CTCCTTTGGG GAAAGCCTGA 
     1141 GGAGTCTCGT GTCTCTAGCG TCTTGGAGGA AAGCAAGCGC CTGGTGGACA CCGCCATGTA 
     1201 CGCCACGATG CAGAGAAACC TCAAGAAAAG AGGAATCCTT TCTCCAGCTC AGCTTCTGTC 
     1261 TTTTTCCAAA CTTCCTGAGC CAACAAGCGG AGTGATTGCC CGAGCAGCAG AGATAATGGA 
     1321 AACATCAATA CAAGCGATGA AAAGAAAAGT CAACCTGAAA ACTCAACAAT CACAGCATCC 
     1381 AACGGATGCT TTATCAGAAG ATCTGCTGAG CATCATTGCA AACATGTCTG GATGTCTCCC 
     1441 TTACATGCTG CCCCCAAAAT GCCCAAACAC TTGCCTGGCG AACAAATACA GGCCCATCAC 
     1501 AGGAGCTTGC AACAACAGAG ACCACCCCAG ATGGGGCGCC TCCAACACGG CCCTGGCACG 
     1561 ATGGCTCCCT CCAGTCTATG AGGACGGCTT CAGTCAGCCC CGAGGCTGGA ACCCCGGCTT 
     1621 CTTGTACAAC GGGTTCCCAC TGCCCCCGGT CCGGGAGGTG ACAAGACATG TCATTCAAGT 
     1681 TTCAAATGAG GTTGTCACAG ATGATGACCG CTATTCTGAC CTCCTGATGG CATGGGGACA 
     1741 ATACATCGAC CACGACATCG CGTTCACACC ACAGAGCACC AGCAAAGCTG CCTTCGGGGG 
     1801 AGGGGCTGAC TGCCAGATGA CTTGTGAGAA CCAAAACCCA TGTTTTCCCA TACAACTCCC 
     1861 GGAGGAGGCC CGGCCGGCCG CGGGCACCGC CTGTCTGCCC TTCTACCGCT CTTCGGCCGC 
     1921 CTGCGGCACC GGGGACCAAG GCGCGCTCTT TGGGAACCTG TCCACGGCCA ACCCGCGGCA 
     1981 GCAGATGAAC GGGTTGACCT CGTTCCTGGA CGCGTCCACC GTGTATGGCA GCTCCCCGGC 
     2041 CCTAGAGAGG CAGCTGCGGA ACTGGACCAG TGCCGAAGGG CTGCTCCGCG TCCACGCGCG 
     2101 CCTCCGGGAC TCCGGCCGCG CCTACCTGCC CTTCGTGCCG CCACGCGCGC CTGCGGCCTG 
     2161 TGCGCCCGAG CCCGGCATCC CCGGAGAGAC CCGCGGGCCC TGCTTCCTGG CCGGAGACGG 
     2221 CCGCGCCAGC GAGGTCCCCT CCCTGACGGC ACTGCACACG CTGTGGCTGC GCGAGCACAA 
     2281 CCGCCTGGCC GCGGCGCTCA AGGCCCTCAA TGCGCACTGG AGCGCGGACG CCGTGTACCA 
     2341 GGAGGCGCGC AAGGTCGTGG GCGCTCTGCA CCAGATCATC ACCCTGAGGG ATTACATCCC 
     2401 CAGGATCCTG GGACCCGAGG CCTTCCAGCA GTACGTGGGT CCCTATGAAG GCTATGACTC 
     2461 CACCGCCAAC CCCACTGTGT CCAACGTGTT CTCCACAGCC GCCTTCCGCT TCGGCCATGC 
     2521 CACGATCCAC CCGCTGGTGA GGAGGCTGGA CGCCAGCTTC CAGGAGCACC CCGACCTGCC 
     2581 CGGGCTGTGG CTGCACCAGG CTTTCTTCAG CCCATGGACA TTACTCCGTG GAGGTGGTTT 
     2641 GGACCCACTA ATACGAGGCC TTCTTGCAAG ACCAGCCAAA CTGCAGGTGC AGGATCAGCT 
     2701 GATGAACGAG GAGCTGACGG AAAGGCTCTT TGTGCTGTCC AATTCCAGCA CCTTGGATCT 
     2761 GGCGTCCATC AACCTGCAGA GGGGCCGGGA CCACGGGCTG CCAGGTTACA ATGAGTGGAG 
     2821 GGAGTTCTGC GGCCTGCCTC GCCTGGAGAC CCCCGCTGAC CTGAGCACAG CCATCGCCAG 
     2881 CAGGAGCGTG GCCGACAAGA TCCTGGACTT GTACAAGCAT CCTGACAACA TCGATGTCTG 
     2941 GCTGGGAGGC TTAGCTGAAA ACTTCCTCCC CAGGGCTCGG ACAGGGCCCC TGTTTGCCTG 
     3001 TCTCATTGGG AAGCAGATGA AGGCTCTGCG GGACGGTGAC TGGTTTTGGT GGGAGAACAG 
     3061 CCACGTCTTC ACGGATGCAC AGAGGCGTGA GCTGGAGAAG CACTCCCTGT CTCGGGTCAT 
     3121 CTGTGACAAC ACTGGCCTCA CCAGGGTGCC CATGGATGCC TTCCAAGTCG GCAAATTCCC 
     3181 CGAAGACTTT GAGTCTTGTG ACAGCATCAC TGGCATGAAC CTGGAGGCCT GGAGGGAAAC 
     3241 CTTTCCTCAA GACGACAAGT GTGGCTTCCC AGAGAGCGTG GAGAATGGGG ACTTTGTGCA 
     3301 CTGTGAGGAG TCTGGGAGGC GCGTGCTGGT GTATTCCTGC CGGCACGGGT ATGAGCTCCA 
     3361 AGGCCGGGAG CAGCTCACTT GCACCCAGGA AGGATGGGAT TTCCAGCCTC CCCTCTGCAA 
     3421 AGACTCCGGG AGGCTCCCTC GGGTGACTTG GATCTCCATG TCGCTGGCTG CTCTGCTGAT 
     3481 CGGAGGCTTC GCAGGTCTCA CCTCGACGGT GATTTGCAGG TGGACACGCA CTGGCACTAA 
     3541 ATCCACACTG CCCATCTCGG AGACAGGCGG AGGAACTCCC GAGCTGAGAT GCGGAAAGCA 
     3601 CCAGGCCGTA GGGACCTCAC CGCAGCGGGC CGCAGCTCAG GACTCGGAGC AGGAGAGTGC 
     3661 TGGGATGGAA GGCCGGGATA CTCACAGGCT GCCGAGAGCC CTCGCGGCCG CTCGAGATGG 
     3721 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3781 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3841 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3901 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3961 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     4021 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     4081 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     4141 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     4201 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     4261 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     4321 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     4381 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     4441 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4501 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4561 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4621 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4681 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4741 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4801 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4861 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4921 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4981 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5041 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     5101 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5161 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     5221 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     5281 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     5341 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     5401 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     5461 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     5521 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     5581 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     5641 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5701 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5761 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5821 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5881 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5941 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     6001 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     6061 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     6121 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     6181 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     6241 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     6301 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     6361 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     6421 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     6481 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     6541 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     6601 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6661 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6721 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6781 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6841 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6901 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6961 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     7021 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     7081 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     7141 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     7201 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     7261 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     7321 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     7381 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     7441 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     7501 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     7561 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     7621 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7681 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7741 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7801 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7861 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7921 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7981 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     8041 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     8101 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     8161 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     8221 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     8281 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     8341 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     8401 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     8461 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     8521 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     8581 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     8641 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8701 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8761 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8821 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8881 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8941 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     9001 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     9061 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     9121 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     9181 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//