LOCUS       RG222412                8204 bp    DNA     circular  SYN 
DEFINITION  ARHGAP27 (tGFP-tagged) - Human Rho GTPase activating protein 27 (ARHGAP27), transcript variant 1
ACCESSION   NM_199282
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2685..3389 
                     /label="GFP"  
     misc_feature    3436..4021 
                     /label="PolyA signal"  
     misc_feature    6113..6758 
                     /label="ColE1"  
     misc_feature    4475..5269 
                     /label="Neo"  
     misc_feature    4106..4408 
                     /label="SV40 ori"  
     misc_feature    6773..7633 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2664..2670 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2664 
                     /label="ARHGAP27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCCGGGC CTGAGCCCTG GCAGCCCAGG GGACCCGCGG CCCCCCACTC 
     1081 CCGAGACGGA CTACCCCGAG TCGCTGACCA GTTACCCCGA GGAGGACTAT TCTCCCGTGG 
     1141 GCTCTTTCGG TGAGCCCGGC CCTACCTCTC CCTTGACCAC ACCCCCCGGC TGGTCTTGTC 
     1201 ATGTCAGCCA GGACAAGCAG ATGCTCTACA CCAACCACTT CACTCAGGAG CAGTGGGTGA 
     1261 GGCTGGAGGA CCCCCACGGG AAGCCATACT TCTACAATCC AGAGGACTCC TCTGTTCGAT 
     1321 GGGAGCTGCC CCAGGTCCCT GTCCCTGCCC CTCGAAGCAT CCATAAATCC AGCCAGGATG 
     1381 GTGACACCCC AGCCCAGGCC AGCCCTCCAG AGGAGAAGGT CCCAGCAGAG CTGGATGAGG 
     1441 TTGGGAGCTG GGAGGAAGTC TCTCCTGCCA CAGCTGCTGT GAGGACCAAG ACCTTGGACA 
     1501 AGGCAGGGGT GCTCCATCGC ACCAAGACGG CAGACAAGGG AAAGCGGCTC CGGAAGAAGC 
     1561 ACTGGAGTGC CTCCTGGACT GTGCTGGAGG GTGGCGTCCT GACATTCTTC AAGGACTCAA 
     1621 AGACCTCGGC TGCAGGCGGC CTGAGGCAGC CTTCCAAGTT TTCCACCCCT GAGTACACAG 
     1681 TGGAGCTGAG GGGGGCCACT CTCTCCTGGG CCCCCAAAGA CAAATCCAGT AGGAAGAATG 
     1741 TGCTGGAGCT ACGGAGCCGA GATGGCTCTG AGTACCTGAT CCAGCACGAC TCGGAGGCCA 
     1801 TCATCAGCAC CTGGCATAAG GCCATTGCTC AGGGCATCCA GGAGCTGTCC GCAGAGCTGC 
     1861 CCCCAGAGGA GAGCGAGAGC AGCAGAGTGG ACTTCGGGTC GAGCGAGCGC TTGGGAAGCT 
     1921 GGCAGGAGAA AGAGGAGGAC GCGCGACCGA ATGCAGCCGC GCCCGCCCTG GGCCCCGTGG 
     1981 GCCTGGAGAG CGACTTGAGC AAGGTCCGGC ACAAGCTCCG CAAGTTCCTC CAGAGGCGGC 
     2041 CCACACTGCA GTCGCTGCGG GAGAAGGGCT ACATCAAAGA CCAGGTGTTC GGCTGCGCGC 
     2101 TGGCCGCGCT GTGTGAGCGC GAGAGGAGCC GGGTGCCACG CTTCGTGCAG CAGTGCATCC 
     2161 GCGCCGTCGA GGCCCGCGGG CTGGACATCG ACGGGCTGTA CCGCATCAGT GGAAACCTGG 
     2221 CCACCATCCA GAAGCTACGC TATAAGGTGG ACCACGATGA GCGCCTTGAC CTGGATGACG 
     2281 GGCGCTGGGA GGACGTCCAC GTTATCACCG GAGCCCTGAA GCTCTTCTTT CGGGAGCTGC 
     2341 CCGAGCCCCT CTTCCCCTTC TCGCACTTCC GCCAGTTCAT TGCGGCCATC AAGTTGCAGG 
     2401 ACCAGGCCCG GCGCAGCCGC TGTGTGCGTG ACTTGGTGCG CTCGCTGCCC GCTCCCAACC 
     2461 ACGACACTCT GCGGATGCTC TTCCAGCACC TCTGCCGGGT GATCGAGCAC GGCGAGCAGA 
     2521 ACCGCATGTC GGTGCAGAGC GTGGCCATTG TGTTCGGGCC CACGCTGCTG CGGCCCGAGG 
     2581 TGGAAGAGAC CAGCATGCCC ATGACCATGG TGTTCCAGAA CCAGGTGGTG GAGCTCATCC 
     2641 TGCAGCAGTG CGCGGACATC TTCCCGCCGC ACACGCGTAC GCGGCCGCTC GAGATGGAGA 
     2701 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     2761 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     2821 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     2881 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     2941 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     3001 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     3061 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     3121 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     3181 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     3241 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     3301 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     3361 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3421 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3481 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3541 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3601 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3661 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3721 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3781 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3841 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3901 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3961 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4021 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     4081 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     4141 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4201 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     4261 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     4321 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     4381 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     4441 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     4501 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     4561 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     4621 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     4681 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     4741 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     4801 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     4861 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     4921 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     4981 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     5041 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     5101 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     5161 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     5221 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     5281 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     5341 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     5401 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     5461 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     5521 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     5581 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     5641 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     5701 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     5761 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     5821 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     5881 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     5941 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     6001 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     6061 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     6121 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     6181 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     6241 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     6301 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     6361 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     6421 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     6481 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     6541 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     6601 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     6661 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     6721 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     6781 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     6841 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     6901 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     6961 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     7021 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     7081 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     7141 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     7201 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     7261 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     7321 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     7381 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     7441 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     7501 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     7561 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     7621 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     7681 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     7741 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     7801 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     7861 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     7921 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     7981 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     8041 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     8101 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     8161 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//