LOCUS       RG222234                9458 bp    DNA     circular  SYN 
DEFINITION  NBR1 (tGFP-tagged) - Human neighbor of BRCA1 gene 1 (NBR1), transcript variant 2
ACCESSION   NM_031858
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3939..4643 
                     /label="GFP"  
     misc_feature    4690..5275 
                     /label="PolyA signal"  
     misc_feature    7367..8012 
                     /label="ColE1"  
     misc_feature    5729..6523 
                     /label="Neo"  
     misc_feature    5360..5662 
                     /label="SV40 ori"  
     misc_feature    8027..8887 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3918..3924 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3918 
                     /label="NBR1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCACAG GTTACTCTAA ATGTGACTTT TAAAAATGAA ATTCAAAGCT 
     1081 TTCTGGTTTC TGATCCAGAA AATACAACTT GGGCTGATAT CGAAGCTATG GTAAAAGTTT 
     1141 CATTTGATCT GAATACTATT CAAATAAAAT ACCTGGATGA GGAAAATGAA GAGGTATCCA 
     1201 TCAACAGTCA AGGAGAATAT GAAGAAGCGC TTAAGATGGC AGTTAAACAG GGAAACCAAC 
     1261 TGCAGATGCA AGTCCACGAA GGGCACCATG TCGTTGATGA AGCCCCACCC CCAGTTGTAG 
     1321 GAGCAAAACG ACTAGCTGCC AGGGCAGGGA AGAAGCCACT TGCACATTAC TCTTCACTGG 
     1381 TGAGAGTCTT GGGATCAGAC ATGAAGACCC CAGAGGATCC TGCAGTGCAG TCGTTTCCAC 
     1441 TTGTTCCATG TGACACAGAC CAGCCTCAAG ACAAGCCCCC AGACTGGTTC ACAAGCTACC 
     1501 TGGAGACGTT CAGAGAACAA GTGGTTAACG AAACGGTTGA GAAGCTTGAA CAGAAATTAC 
     1561 ATGAAAAGCT TGTCCTCCAG AACCCATCCT TGGGTTCTTG TCCCTCAGAA GTCTCAATGC 
     1621 CTACTTCAGA AGAAACATTG TTTTTGCCAG AAAACCAGTT CAGCTGGCAT ATTGCTTGCA 
     1681 ACAACTGCCA AAGAAGGATT GTTGGTGTCC GCTACCAGTG TAGCCTATGC CCATCCTACA 
     1741 ATATCTGTGA AGATTGTGAA GCAGGGCCAT ATGGCCATGA CACTAACCAC GTCCTGCTGA 
     1801 AGTTGCGGAG ACCTGTTGTG GGCTCCTCTG AACCGTTCTG TCACTCAAAG TACTCTACTC 
     1861 CTCGTCTTCC TGCTGCTCTG GAACAAGTCA GGCTCCAGAA ACAGGTTGAT AAGAACTTTC 
     1921 TTAAAGCAGA AAAGCAAAGG TTGCGAGCTG AGAAGAAACA ACGTAAAGCA GAGGTCAAGG 
     1981 AACTTAAAAA GCAGCTTAAA CTCCATAGGA AAATTCACCT GTGGAATTCA ATCCATGGAC 
     2041 TCCAGAGCCC CAAGTCTCCT TTAGGCCGAC CTGAGAGCTT GCTCCAGTCT AATACCCTGA 
     2101 TGCTCCCTTT GCAGCCCTGT ACCTCCGTTA TGCCAATGCT CAGTGCAGCA TTTGTGGATG 
     2161 AGAATTTGCC TGATGGGACT CACCTTCAGC CAGGAACCAA GTTTATCAAA CACTGGAGGA 
     2221 TGAAAAATAC AGGAAATGTA AAGTGGAGTG CAGACACAAA GCTCAAGTTC ATGTGGGGAA 
     2281 ACCTGACTTT GGCTTCCACA GAAAAGAAGG ATGTTTTGGT TCCCTGCCTC AAGGCCGGCC 
     2341 ATGTGGGAGT TGTATCTGTG GAGTTCATTG CCCCAGCCTT GGAGGGAACG TATACTTCCC 
     2401 ATTGGCGTCT TTCTCACAAA GGCCAGCAAT TTGGGCCTCG GGTCTGGTGC AGTATCATAG 
     2461 TAGATCCTTT CCCCTCCGAA GAGAGCCCTG ATAACATTGA AAAGGGCATG ATCAGCTCAA 
     2521 GCAAAACTGA TGATCTCACC TGCCAGCAAG AGGAAACTTT TCTTCTGGCT AAAGAAGAAA 
     2581 GACAGCTTGG TGAAGTGACT GAGCAGACAG AAGGGACAGC AGCCTGCATC CCACAGAAGG 
     2641 CAAAAAATGT TGCCAGTGAG AGGGAGCTCT ACATCCCATC TGTGGATCTT CTGACTGCCC 
     2701 AGGACCTGCT GTCCTTTGAG CTGTTGGATA TAAACATTGT TCAAGAGTTG GAGAGAGTGC 
     2761 CCCACAACAC CCCTGTGGAT GTGACTCCCT GCATGTCTCC TCTGCCACAT GACAGTCCTT 
     2821 TAATAGAGAA GCCAGGCTTG GGGCAGATAG AGGAAGAGAA TGAAGGGGCA GGATTTAAAG 
     2881 CACTTCCTGA TTCTATGGTG TCAGTAAAGA GGAAGGCTGA GAACATTGCT TCTGTGGAGG 
     2941 AAGCAGAAGA AGACCTGAGT GGGACCCAGT TTGTGTGTGA GACAGTAATC CGATCCCTTA 
     3001 CCTTGGATGC TGCCCCAGAC CACAACCCTC CTTGCAGACA GAAGTCCTTG CAGATGACAT 
     3061 TTGCCTTGCC TGAAGGACCA CTTGGAAATG AGAAGGAGGA GATTATCCAT ATCGCTGAGG 
     3121 AAGAAGCTGT CATGGAGGAG GAGGAGGATG AGGAGGATGA GGAGGAGGAG GATGAGCTCA 
     3181 AAGATGAAGT TCAAAGTCAG TCCTCTGCTT CCTCAGAGGA TTACATCATC ATCCTGCCTG 
     3241 AGTGCTTTGA TACCAGCCGC CCCCTGGGGG ATTCTATGTA CAGCTCTGCG CTCTCACAGC 
     3301 CAGGCCTGGA GCGAGGTGCT GAAGGCAAGC CTGGGGTTGA GGCTGGGCAG GAACCAGCTG 
     3361 AGGCTGGGGA AAGACTCCCT GGAGGGGAGA ACCAGCCACA GGAGCACAGC ATAAGTGACA 
     3421 TCCTCACGAC CTCACAGACT CTGGAAACAG TGCCCCTAAT CCCAGAGGTA GTGGAGCTTC 
     3481 CACCGTCACT GCCCAGGAGC TCTCCTTGTG TACATCATCA TGGTTCCCCA GGAGTGGATT 
     3541 TACCAGTTAC CATACCAGAA GTTTCTTCAG TCCCTGATCA GATCAGAGGA GAGCCCAGAG 
     3601 GCTCATCAGG ACTTGTAAAC AGCAGACAGA AGAGCTATGA CCACTCAAGG CACCATCATG 
     3661 GGAGCAGCAT TGCTGGAGGA CTGGTGAAGG GGGCTTTGTC TGTTGCTGCC TCTGCATACA 
     3721 AGGCCCTGTT TGCTGGGCCA CCAGTCACTG CACAGCCAAT AATTTCTGAA GATCAGACAG 
     3781 CAGCCCTGAT GGCCCATCTC TTTGAAATGG GATTCTGTGA CAGGCAGCTG AACCTACGGC 
     3841 TGCTGAAGAA ACACAATTAC AATATCCTGC AGGTTGTGAC AGAACTTCTT CAGTTAAACA 
     3901 ACAACGACTG GTACAGCCAA CGCTATACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3961 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     4021 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     4081 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     4141 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4201 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4261 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4321 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4381 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4441 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4501 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4561 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4621 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4681 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4741 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4801 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4861 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4921 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4981 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5041 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5101 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5161 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5221 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5281 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5341 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5401 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5461 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5521 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5581 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5641 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5701 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5761 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5821 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5881 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5941 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     6001 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     6061 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     6121 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6181 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6241 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6301 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6361 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6421 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6481 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6541 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6601 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6661 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6721 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6781 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6841 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6901 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6961 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     7021 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     7081 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     7141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7801 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7861 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7921 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7981 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     8041 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     8101 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     8161 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8221 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8281 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8341 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8401 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8461 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8521 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8581 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8641 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8701 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8761 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8821 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8881 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8941 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     9001 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     9061 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     9121 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9181 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9241 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9301 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9361 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9421 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//