LOCUS       RG221945                9041 bp    DNA     circular  SYN 
DEFINITION  BICD2 (tGFP-tagged) - Human bicaudal D homolog 2 (Drosophila) (BICD2), transcript variant 2
ACCESSION   NM_015250
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3520..4224 
                     /label="GFP"  
     misc_feature    4271..4856 
                     /label="PolyA signal"  
     misc_feature    6948..7593 
                     /label="ColE1"  
     misc_feature    5310..6104 
                     /label="Neo"  
     misc_feature    4941..5243 
                     /label="SV40 ori"  
     misc_feature    7608..8468 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3492..3499 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3492 
                     /label="BICD2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCGCCG TCGGAGGAGG AGGAGTACGC GCGGCTGGTG ATGGAGGCGC 
     1081 AGCCGGAGTG GCTGCGCGCC GAGGTGAAGC GGCTGTCCCA CGAGCTGGCC GAGACCACGC 
     1141 GTGAGAAGAT CCAGGCGGCC GAGTACGGGC TGGCGGTGCT CGAGGAGAAG CACCAGCTCA 
     1201 AGCTGCAGTT CGAGGAGCTC GAGGTGGACT ATGAGGCTAT CCGCAGCGAG ATGGAGCAGC 
     1261 TCAAGGAGGC CTTTGGACAA GCACACACAA ACCACAAGAA GGTGGCTGCT GACGGAGAGA 
     1321 GCCGGGAGGA GAGCCTGATC CAGGAGTCGG CCTCCAAGGA GCAGTACTAC GTGCGGAAGG 
     1381 TGCTAGAGCT GCAGACGGAG CTGAAGCAGT TGCGCAATGT CCTCACCAAC ACGCAGTCGG 
     1441 AGAATGAGCG CCTGGCCTCT GTGGCCCAGG AGCTGAAGGA GATCAACCAG AATGTGGAGA 
     1501 TCCAGCGTGG CCGCCTGCGG GATGACATCA AGGAGTACAA ATTCCGGGAA GCTCGTCTGC 
     1561 TGCAGGACTA CTCGGAACTG GAGGAGGAGA ACATCAGCCT GCAGAAGCAA GTGTCTGTGC 
     1621 TCAGACAGAA CCAGGTGGAG TTTGAGGGCC TCAAGCATGA GATCAAGCGT CTGGAGGAGG 
     1681 AGACCGAGTA CCTCAACAGC CAGCTGGAGG ATGCCATCCG CCTCAAGGAG ATCTCAGAGC 
     1741 GGCAGCTGGA GGAGGCGCTG GAGACCCTGA AGACGGAGCG CGAACAGAAG AACAGCCTGC 
     1801 GCAAGGAGCT GTCACACTAC ATGAGCATCA ATGACTCCTT CTACACCAGC CACCTGCATG 
     1861 TCTCGCTGGA TGGCCTCAAG TTCAGTGACG ATGCTGCCGA GCCCAACAAC GATGCCGAGG 
     1921 CCCTGGTCAA TGGCTTTGAG CACGGCGGCC TGGCCAAGCT GCCACTGGAC AACAAGACCT 
     1981 CCACGCCCAA GAAGGAGGGC CTCGCACCGC CCTCCCCCAG CCTCGTCTCC GACCTACTCA 
     2041 GTGAGCTCAA CATCTCTGAG ATCCAGAAGC TGAAGCAGCA GCTGATGCAG ATGGAGCGGG 
     2101 AAAAGGCGGG CCTGCTGGCA ACGCTGCAGG ACACACAGAA GCAGCTGGAG CACACGCGGG 
     2161 GCTCCCTGTC AGAACAGCAG GAGAAGGTGA CCCGCCTCAC AGAGAATCTG AGTGCCCTGC 
     2221 GGCGCCTGCA GGCCAGCAAG GAGCGGCAGA CAGCCCTGGA CAACGAGAAG GACCGTGACA 
     2281 GCCATGAGGA TGGGGACTAC TACGAGGTGG ACATCAACGG GCCTGAGATC TTGGCCTGCA 
     2341 AGTACCATGT GGCTGTGGCT GAGGCTGGCG AGCTCCGCGA GCAGCTCAAG GCACTGCGCA 
     2401 GCACGCACGA GGCTCGTGAG GCCCAGCACG CCGAGGAGAA GGGCCGCTAT GAGGCTGAGG 
     2461 GCCAGGCACT CACGGAGAAG GTCTCCCTGC TAGAGAAGGC CAGCCGCCAG GACCGCGAGC 
     2521 TGCTGGCCCG GCTGGAGAAG GAGCTAAAGA AGGTGAGCGA CGTCGCCGGC GAGACACAGG 
     2581 GCAGCCTGAG TGTGGCCCAG GATGAGCTGG TGACCTTCAG TGAGGAGCTG GCCAATCTCT 
     2641 ACCACCACGT GTGCATGTGC AACAATGAGA CACCCAACCG TGTCATGCTG GACTACTACC 
     2701 GCGAGGGCCA GGGCGGGGCC GGCCGCACCA GTCCCGGGGG CCGCACCAGC CCCGAGGCGC 
     2761 GTGGCCGGCG CTCACCCATC CTCCTACCCA AGGGGCTGCT GGCTCCTGAG GCGGGCCGAG 
     2821 CAGATGGTGG GACGGGGGAC AGCAGCCCCT CGCCTGGCTC CTCACTGCCA TCACCCCTGA 
     2881 GTGACCCACG CCGGGAGCCC ATGAACATCT ACAACCTGAT CGCTATCATC CGTGACCAGA 
     2941 TCAAGCACCT GCAGGCAGCC GTGGACCGCA CCACGGAGCT GTCACGCCAG CGCATTGCCT 
     3001 CTCAGGAGCT GGGCCCCGCC GTGGACAAGG ACAAGGAAGC GCTTATGGAG GAGATCCTCA 
     3061 AGCTGAAGTC GCTGCTCAGC ACCAAGCGGG AGCAGATCAC CACGCTGCGC ACTGTGCTCA 
     3121 AGGCCAACAA GCAGACGGCC GAGGTGGCCC TTGCCAACCT GAAGAGCAAG TATGAGAATG 
     3181 AGAAGGCCAT GGTTACCGAG ACCATGATGA AGCTGCGCAA TGAGCTCAAG GCCCTCAAGG 
     3241 AGGACGCAGC CACCTTCTCC TCGCTGCGTG CTATGTTTGC CACCAGGTGT GACGAGTACA 
     3301 TTACACAGCT GGATGAGATG CAGCGGCAGC TGGCGGCTGC TGAGGACGAG AAGAAGACGC 
     3361 TGAACTCGCT GCTGCGCATG GCCATCCAGC AGAAGCTGGC GCTGACCCAG CGGCTGGAGC 
     3421 TGCTCGAGCT GGACCATGAG CAGACCCGGC GTGGCCGTGC CAAAGCCGCC CCGAAGACCA 
     3481 AGCCAGCCAC ACCGAGCCTG AGCGGACCGA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3541 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3601 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3661 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3721 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3781 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3841 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     3901 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     3961 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4021 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4081 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4141 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4201 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4261 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4321 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4381 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4441 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4501 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4561 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4621 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4681 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4741 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4801 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4861 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     4921 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4981 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5041 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5101 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5161 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5221 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5281 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5341 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5401 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5461 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5521 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5581 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5641 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5701 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5761 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5821 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     5881 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     5941 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6001 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6061 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6121 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6181 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6241 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6301 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6361 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6421 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6481 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6541 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6601 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6661 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6721 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6781 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6841 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6901 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     6961 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7021 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7081 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7141 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7201 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7261 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7321 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7381 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7441 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7501 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7561 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7621 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7681 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7741 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7801 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7861 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     7921 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     7981 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8041 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8101 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8161 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8221 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8281 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8341 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8401 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8461 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8521 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8581 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8641 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8701 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8761 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8821 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     8881 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     8941 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9001 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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