LOCUS       RG220526                9482 bp    DNA     circular  SYN 
DEFINITION  ARHGAP6 (tGFP-tagged) - Human Rho GTPase activating protein 6 (ARHGAP6), transcript variant 1
ACCESSION   NM_013427
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3963..4667 
                     /label="GFP"  
     misc_feature    4714..5299 
                     /label="PolyA signal"  
     misc_feature    7391..8036 
                     /label="ColE1"  
     misc_feature    5753..6547 
                     /label="Neo"  
     misc_feature    5384..5686 
                     /label="SV40 ori"  
     misc_feature    8051..8911 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3942..3948 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3942 
                     /label="ARHGAP6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGCGCAG AGCCTGCTCC ACAGCGTCTT CTCCTGTTCC TCGCCCGCTT 
     1081 CAAGTAGCGC GGCCTCGGCC AAGGGCTTCT CCAAGAGGAA GCTGCGCCAG ACCCGCAGCC 
     1141 TGGACCCGGC CCTGATCGGC GGCTGCGGGA GCGACGAGGC GGGCGCGGAG GGCAGTGCGC 
     1201 GGGGAGCCAC GGCGGGCCGC CTCTACTCCC CATCACTCCC AGCCGAGAGT CTCGGCCCTC 
     1261 GCTTGGCGTC CTCTTCCCGG GGTCCGCCCC CCAGGGCCAC CAGGCTACCG CCTCCTGGAC 
     1321 CTCTTTGCTC GTCCTTCTCC ACACCCAGCA CCCCGCAGGA GAAGTCACCA TCCGGCAGCT 
     1381 TTCACTTTGA CTATGAGGTT CCCCTGGGTC GCGGCGGCCT CAAGAAGAGC ATGGCCTGGG 
     1441 ACCTGCCTTC TGTCCTGGCC GGGCCAGCCA GTAGCCGAAG CGCTTCCAGC ATCCTCTGTT 
     1501 CATCCGGGGG AGGCCCCAAT GGCATCTTCG CTTCTCCTAG GAGGTGGCTC CAGCAGAGGA 
     1561 AGTTCCAGTC CCCACCCGAC AGTCGCGGGC ACCCCTACGT CGTGTGGAAA TCCGAGGGTG 
     1621 ATTTCACCTG GAACAGCATG TCAGGCCGCA GTGTACGGCT GAGGTCAGTC CCCATCCAGA 
     1681 GTCTCTCAGA GCTGGAGAGG GCCCGGCTGC AGGAAGTGGC TTTTTATCAG TTGCAACAGG 
     1741 ACTGTGACCT GAGCTGTCAG ATCACCATTC CCAAAGATGG ACAAAAGAGA AAGAAATCTT 
     1801 TAAGAAAGAA ACTGGATTCA CTAGGAAAGG AGAAAAACAA AGACAAAGAA TTCATCCCAC 
     1861 AGGCATTTGG AATGCCCTTA TCCCAAGTCA TTGCGAATGA CAGGGCCTAT AAACTCAAGC 
     1921 AGGACTTGCA GAGGGACGAG CAGAAAGATG CATCTGACTT TGTGGCTTCC CTCCTCCCAT 
     1981 TTGGAAATAA AAGACAAAAC AAAGAACTCT CAAGCAGTAA CTCATCTCTC AGCTCAACCT 
     2041 CAGAAACACC GAATGAGTCA ACGTCCCCAA ACACCCCGGA ACCGGCTCCT CGGGCTAGGA 
     2101 GGAGGGGTGC CATGTCAGTG GATTCTATCA CCGATCTTGA TGACAATCAG TCTCGACTAC 
     2161 TAGAAGCTTT ACAACTTTCC TTGCCTGCTG AGGCTCAAAG TAAAAAGGAA AAAGCCAGAG 
     2221 ATAAGAAACT CAGTCTGAAT CCTATTTACA GACAGGTCCC TAGGCTGGTG GACAGCTGCT 
     2281 GTCAGCACCT AGAAAAACAT GGCCTCCAGA CAGTGGGGAT ATTCCGAGTT GGAAGCTCAA 
     2341 AAAAGAGAGT GAGACAATTA CGTGAGGAAT TTGACCGTGG GATTGATGTC TCTCTGGAGG 
     2401 AGGAGCACAG TGTTCATGAT GTGGCAGCCT TGCTGAAAGA GTTCCTGAGG GACATGCCAG 
     2461 ACCCCCTTCT CACCAGGGAG CTGTACACAG CTTTCATCAA CACTCTCTTG TTGGAGCCGG 
     2521 AGGAACAGCT GGGCACCTTG CAGCTCCTCA TATACCTTCT ACCTCCCTGC AACTGCGACA 
     2581 CCCTCCACCG CCTGCTACAG TTCCTCTCCA TCGTGGCCAG GCATGCCGAT GACAACATCA 
     2641 GCAAAGATGG GCAAGAGGTC ACTGGGAATA AAATGACATC TCTAAACTTA GCCACCATAT 
     2701 TTGGACCCAA CCTGCTGCAC AAGCAGAAGT CATCAGACAA AGAATTCTCA GTTCAGAGTT 
     2761 CAGCCCGGGC TGAGGAGAGC ACGGCCATCA TCGCTGTTGT GCAAAAGATG ATTGAAAATT 
     2821 ATGAAGCCCT GTTCATGGTT CCCCCAGATC TCCAGAACGA AGTGCTGATC AGCCTGTTAG 
     2881 AGACCGATCC TGATGTCGTG GACTATTTAC TCAGAAGAAA GGCTTCCCAA TCATCAAGCC 
     2941 CTGACATGCT GCAGTCGGAA GTTTCCTTTT CCGTGGGAGG GAGGCATTCA TCTACAGACT 
     3001 CCAACAAGGC CTCCAGCGGA GACATCTCCC CTTATGACAA CAACTCCCCA GTGCTGTCTG 
     3061 AGCGCTCCCT GCTGGCTATG CAAGAGGACG CGGCCCCGGG GGGCTCGGAG AAGCTTTACA 
     3121 GAGTGCCAGG GCAGTTTATG CTGGTGGGCC ACTTGTCGTC GTCAAAGTCA AGGGAAAGTT 
     3181 CTCCTGGACC AAGGCTTGGG AAAGATCTGT CAGAGGAGCC TTTCGATATC TGGGGAACTT 
     3241 GGCATTCAAC ATTAAAAAGC GGATCCAAAG ACCCAGGAAT GACAGGTTCC TCTGGAGACA 
     3301 TTTTTGAAAG CAGCTCCCTA AGAGCGGGGC CCTGCTCCCT TTCTCAAGGG AACCTGTCCC 
     3361 CAAATTGGCC TCGGTGGCAG GGGAGCCCCG CAGAGCTGGA CAGCGACACG CAGGGGGCTC 
     3421 GGAGGACTCA GGCCGCAGCC CCCGCGACGG AGGGCAGGGC CCACCCTGCG GTGTCGCGCG 
     3481 CCTGCAGCAC GCCCCACGTC CAGGTGGCAG GGAAAGCCGA GCGGCCCACG GCCAGGTCGG 
     3541 AGCAGTACTT GACCCTGAGC GGCGCCCACG ACCTCAGCGA GAGTGAGCTG GATGTGGCCG 
     3601 GGCTGCAGAG CCGGGCCACA CCTCAGTGCC AAAGACCCCA TGGGAGTGGG AGGGATGACA 
     3661 AGCGGCCCCC GCCTCCATAC CCGGGCCCAG GGAAGCCCGC GGCAGCGGCA GCCTGGATCC 
     3721 AGGGGCCCCC GGAAGGCGTG GAGACACCCA CGGACCAGGG AGGCCAAGCA GCCGAGCGAG 
     3781 AGCAGCAGGT CACGCAGAAA AAACTGAGCA GCGCCAACTC CCTGCCAGCG GGCGAGCAGG 
     3841 ACAGTCCGCG CCTGGGGGAC GCTGGCTGGC TCGACTGGCA GAGAGAGCGC TGGCAGATCT 
     3901 GGGAGCTCCT GTCGACCGAC AACCCCGATG CCCTGCCCGA GACGCTGGTC ACGCGTACGC 
     3961 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     4021 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     4081 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     4141 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4201 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4261 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4321 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4381 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4441 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4501 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4561 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4621 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5401 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5461 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5521 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5581 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5641 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5701 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5761 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5821 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5881 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5941 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     6001 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     6061 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     6121 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6181 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6241 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6301 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6361 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6421 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6481 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6541 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6601 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6661 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6721 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6781 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6841 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6901 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6961 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     7021 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     7081 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     7141 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7201 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7261 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7321 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7381 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7441 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7501 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7561 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7621 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7681 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7741 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7801 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7861 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7921 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7981 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     8041 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     8101 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     8161 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8221 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8281 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8341 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8401 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8461 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8521 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8581 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8641 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8701 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8761 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8821 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8881 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8941 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     9001 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     9061 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     9121 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9181 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9241 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9301 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9361 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9421 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9481 TT
//