LOCUS       RG220423                9428 bp    DNA     circular  SYN 
DEFINITION  DMD (tGFP-tagged) - Human dystrophin (DMD), transcript variant Dp116
ACCESSION   NM_004014
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3909..4613 
                     /label="GFP"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    7337..7982 
                     /label="ColE1"  
     misc_feature    5699..6493 
                     /label="Neo"  
     misc_feature    5330..5632 
                     /label="SV40 ori"  
     misc_feature    7997..8857 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3888..3894 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3888 
                     /label="DMD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTACACAGG AAGACATACC ATGTAAAGGA CCTCCAAGGT GAAATTGAAG 
     1081 CTCACACAGA TGTTTATCAC AACCTGGATG AAAACAGCCA AAAAATCCTG AGATCCCTGG 
     1141 AAGGTTCCGA TGATGCAGTC CTGTTACAAA GACGTTTGGA TAACATGAAC TTCAAGTGGA 
     1201 GTGAACTTCG GAAAAAGTCT CTCAACATTA GGTCCCATTT GGAAGCCAGT TCTGACCAGT 
     1261 GGAAGCGTCT GCACCTTTCT CTGCAGGAAC TTCTGGTGTG GCTACAGCTG AAAGATGATG 
     1321 AATTAAGCCG GCAGGCACCT ATTGGAGGCG ACTTTCCAGC AGTTCAGAAG CAGAACGATG 
     1381 TACATAGGGC CTTCAAGAGG GAATTGAAAA CTAAAGAACC TGTAATCATG AGTACTCTTG 
     1441 AGACTGTACG AATATTTCTG ACAGAGCAGC CTTTGGAAGG ACTAGAGAAA CTCTACCAGG 
     1501 AGCCCAGAGA GCTGCCTCCT GAGGAGAGAG CCCAGAATGT CACTCGGCTT CTACGAAAGC 
     1561 AGGCTGAGGA GGTCAATACT GAGTGGGAAA AATTGAACCT GCACTCCGCT GACTGGCAGA 
     1621 GAAAAATAGA TGAGACCCTT GAAAGACTCC AGGAACTTCA AGAGGCCACG GATGAGCTGG 
     1681 ACCTCAAGCT GCGCCAAGCT GAGGTGATCA AGGGATCCTG GCAGCCCGTG GGCGATCTCC 
     1741 TCATTGACTC TCTCCAAGAT CACCTCGAGA AAGTCAAGGC ACTTCGAGGA GAAATTGCGC 
     1801 CTCTGAAAGA GAACGTGAGC CACGTCAATG ACCTTGCTCG CCAGCTTACC ACTTTGGGCA 
     1861 TTCAGCTCTC ACCGTATAAC CTCAGCACTC TGGAAGACCT GAACACCAGA TGGAAGCTTC 
     1921 TGCAGGTGGC CGTCGAGGAC CGAGTCAGGC AGCTGCATGA AGCCCACAGG GACTTTGGTC 
     1981 CAGCATCTCA GCACTTTCTT TCCACGTCTG TCCAGGGTCC CTGGGAGAGA GCCATCTCGC 
     2041 CAAACAAAGT GCCCTACTAT ATCAACCACG AGACTCAAAC AACTTGCTGG GACCATCCCA 
     2101 AAATGACAGA GCTCTACCAG TCTTTAGCTG ACCTGAATAA TGTCAGATTC TCAGCTTATA 
     2161 GGACTGCCAT GAAACTCCGA AGACTGCAGA AGGCCCTTTG CTTGGATCTC TTGAGCCTGT 
     2221 CAGCTGCATG TGATGCCTTG GACCAGCACA ACCTCAAGCA AAATGACCAG CCCATGGATA 
     2281 TCCTGCAGAT TATTAATTGT TTGACCACTA TTTATGACCG CCTGGAGCAA GAGCACAACA 
     2341 ATTTGGTCAA CGTCCCTCTC TGCGTGGATA TGTGTCTGAA CTGGCTGCTG AATGTTTATG 
     2401 ATACGGGACG AACAGGGAGG ATCCGTGTCC TGTCTTTTAA AACTGGCATC ATTTCCCTGT 
     2461 GTAAAGCACA TTTGGAAGAC AAGTACAGAT ACCTTTTCAA GCAAGTGGCA AGTTCAACAG 
     2521 GATTTTGTGA CCAGCGCAGG CTGGGCCTCC TTCTGCATGA TTCTATCCAA ATTCCAAGAC 
     2581 AGTTGGGTGA AGTTGCATCC TTTGGGGGCA GTAACATTGA GCCAAGTGTC CGGAGCTGCT 
     2641 TCCAATTTGC TAATAATAAG CCAGAGATCG AAGCGGCCCT CTTCCTAGAC TGGATGAGAC 
     2701 TGGAACCCCA GTCCATGGTG TGGCTGCCCG TCCTGCACAG AGTGGCTGCT GCAGAAACTG 
     2761 CCAAGCATCA GGCCAAATGT AACATCTGCA AAGAGTGTCC AATCATTGGA TTCAGGTACA 
     2821 GGAGTCTAAA GCACTTTAAT TATGACATCT GCCAAAGCTG CTTTTTTTCT GGTCGAGTTG 
     2881 CAAAAGGCCA TAAAATGCAC TATCCCATGG TGGAATATTG CACTCCGACT ACATCAGGAG 
     2941 AAGATGTTCG AGACTTTGCC AAGGTACTAA AAAACAAATT TCGAACCAAA AGGTATTTTG 
     3001 CGAAGCATCC CCGAATGGGC TACCTGCCAG TGCAGACTGT CTTAGAGGGG GACAACATGG 
     3061 AAACTCCCGT TACTCTGATC AACTTCTGGC CAGTAGATTC TGCGCCTGCC TCGTCCCCTC 
     3121 AGCTTTCACA CGATGATACT CATTCACGCA TTGAACATTA TGCTAGCAGG CTAGCAGAAA 
     3181 TGGAAAACAG CAATGGATCT TATCTAAATG ATAGCATCTC TCCTAATGAG AGCATAGATG 
     3241 ATGAACATTT GTTAATCCAG CATTACTGCC AAAGTTTGAA CCAGGACTCC CCCCTGAGCC 
     3301 AGCCTCGTAG TCCTGCCCAG ATCTTGATTT CCTTAGAGAG TGAGGAAAGA GGGGAGCTAG 
     3361 AGAGAATCCT AGCAGATCTT GAGGAAGAAA ACAGGAATCT GCAAGCAGAA TATGACCGTC 
     3421 TAAAGCAGCA GCACGAACAT AAAGGCCTGT CCCCACTGCC GTCCCCTCCT GAAATGATGC 
     3481 CCACCTCTCC CCAGAGTCCC CGGGATGCTG AGCTCATTGC TGAGGCCAAG CTACTGCGTC 
     3541 AACACAAAGG CCGCCTGGAA GCCAGGATGC AAATCCTGGA AGACCACAAT AAACAGCTGG 
     3601 AGTCACAGTT ACACAGGCTA AGGCAGCTGC TGGAGCAACC CCAGGCAGAG GCCAAAGTGA 
     3661 ATGGCACAAC GGTGTCCTCT CCTTCTACCT CTCTACAGAG GTCCGACAGC AGTCAGCCTA 
     3721 TGCTGCTCCG AGTGGTTGGC AGTCAAACTT CGGACTCCAT GGGTGAGGAA GATCTTCTCA 
     3781 GTCCTCCCCA GGACACAAGC ACAGGGTTAG AGGAGGTGAT GGAGCAACTC AACAACTCCT 
     3841 TCCCTAGTTC AAGAGGAAGA AATACCCCTG GAAAGCCAAT GAGAGAGGAC ACAATGACGC 
     3901 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3961 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4021 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4081 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4141 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4201 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4261 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4321 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4381 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4441 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4501 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4561 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5341 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5401 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5461 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5521 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5581 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5641 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5701 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5761 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5821 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5881 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5941 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6001 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6061 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6121 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6181 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6241 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6301 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6361 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6421 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6481 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6541 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7021 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7081 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7141 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7201 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7261 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7321 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7381 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7441 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7501 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7561 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7621 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7681 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7741 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9001 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9061 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9121 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9181 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9241 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9301 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9361 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9421 CGAATTTT
//