LOCUS       RG220304                9362 bp    DNA     circular  SYN 
DEFINITION  PDE11A (tGFP-tagged) - Human phosphodiesterase 11A (PDE11A), transcript variant 4
ACCESSION   NM_016953
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3843..4547 
                     /label="GFP"  
     misc_feature    4594..5179 
                     /label="PolyA signal"  
     misc_feature    7271..7916 
                     /label="ColE1"  
     misc_feature    5633..6427 
                     /label="Neo"  
     misc_feature    5264..5566 
                     /label="SV40 ori"  
     misc_feature    7931..8791 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3822..3828 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3822 
                     /label="PDE11A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCTCC CGCCTGGACT TTGGGGAGGT GGAAACTTTC CTGGACAGGC 
     1081 ACCCAGAGTT GTTTGAAGAT TACTTGATGC GGAAGGGGAA GCAGGAGATG GTTGAAAAGT 
     1141 GGCTGCAGAG GCACAGTCAG GGTCAGGGGG CTTTAGGTCC AAGGCCCTCT TTGGCTGGTA 
     1201 CCAGCAGCTT GGCTCACAGC ACCTGCAGAG GTGGCAGCAG CGTTGGTGGT GGCACTGGAC 
     1261 CAAATGGCTC TGCCCACAGC CAGCCCCTTC CCGGTGGCGG GGACTGTGGT GGGGTTCCCT 
     1321 TGAGTCCCAG CTGGGCCGGT GGCAGCAGGG GCGATGGGAA CCTGCAGCGG AGAGCTTCTC 
     1381 AGAAAGAGCT AAGGAAGAGT TTTGCCCGCT CCAAGGCCAT CCACGTGAAC AGGACCTACG 
     1441 ATGAACAGGT GACCTCCCGG GCTCAGGAAC CCCTGAGTAG TGTACGACGG AGGGCACTTC 
     1501 TCCGGAAGGC AAGCTCCCTG CCCCCCACCA CAGCCCATAT TCTCAGTGCG CTGCTGGAAT 
     1561 CGAGAGTGAA TCTGCCTCAG TATCCCCCTA CAGCCATCGA CTACAAGTGC CATCTGAAAA 
     1621 AGCATAATGA GCGTCAGTTC TTTCTGGAAT TGGTCAAAGA TATCTCCAAT GACCTTGACC 
     1681 TCACCAGCCT GAGCTACAAG ATTCTCATCT TTGTCTGCCT TATGGTGGAT GCTGACCGCT 
     1741 GCTCTCTTTT CCTGGTGGAA GGGGCAGCTG CTGGCAAGAA GACCTTGGTC TCCAAATTCT 
     1801 TTGATGTGCA TGCAGGAACC CCTCTGCTGC CTTGCAGCAG CACAGAGAAC TCAAATGAGG 
     1861 TGCAGGTCCC CTGGGGCAAA GGTATCATTG GCTATGTCGG GGAGCATGGA GAAACGGTCA 
     1921 ACATTCCTGA TGCCTACCAG GATCGACGAT TCAATGATGA AATCGACAAG CTAACTGGAT 
     1981 ACAAGACAAA ATCATTATTG TGCATGCCTA TCCGAAGCAG TGATGGTGAG ATTATTGGTG 
     2041 TGGCCCAAGC GATAAATAAG ATTCCTGAAG GAGCTCCATT TACTGAAGAT GATGAAAAAG 
     2101 TTATGCAGAT GTATCTTCCA TTTTGTGGAA TCGCCATATC TAACGCTCAG CTCTTTGCTG 
     2161 CCTCAAGGAA AGAATATGAA AGAAGCAGAG CTTTGCTAGA GGTGGTTAAT GACCTCTTTG 
     2221 AAGAACAGAC TGACCTGGAG AAAATTGTCA AGAAAATAAT GCATCGGGCC CAAACTCTGC 
     2281 TGAAATGTGA ACGCTGTTCT GTTTTACTCC TAGAGGACAT CGAATCACCA GTGGTGAAAT 
     2341 TTACCAAATC CTTTGAATTG ATGTCCCCAA AGTGCAGTGC TGATGCTGAG AACAGTTTCA 
     2401 AAGAAAGCAT GGAGAAATCA TCATACTCCG ACTGGCTAAT AAATAACAGC ATTGCTGAGC 
     2461 TGGTTGCTTC AACAGGCCTT CCAGTGAACA TCAGTGATGC CTACCAGGAT CCGCGCTTTG 
     2521 ATGCAGAGGC AGACCAGATA TCTGGTTTTC ACATAAGATC TGTTCTTTGT GTCCCTATTT 
     2581 GGAATAGCAA CCACCAAATA ATTGGAGTGG CTCAAGTGTT AAACAGACTT GATGGGAAAC 
     2641 CTTTTGATGA TGCAGATCAA CGACTTTTTG AGGCTTTTGT CATCTTTTGT GGACTTGGCA 
     2701 TCAACAACAC AATTATGTAT GATCAAGTGA AGAAGTCCTG GGCCAAGCAG TCTGTGGCTC 
     2761 TTGATGTGCT ATCATACCAT GCAACATGTT CAAAAGCTGA AGTTGACAAG TTTAAGGCAG 
     2821 CCAACATCCC TCTGGTGTCA GAACTTGCCA TCGATGACAT TCATTTTGAT GACTTTTCTC 
     2881 TCGACGTTGA TGCCATGATC ACAGCTGCTC TCCGGATGTT CATGGAGCTG GGGATGGTAC 
     2941 AGAAATTTAA AATTGACTAT GAGACACTGT GTAGGTGGCT TTTGACAGTG AGGAAAAACT 
     3001 ATCGGATGGT TCTATACCAC AACTGGAGAC ATGCCTTCAA CGTGTGTCAG CTGATGTTCG 
     3061 CGATGTTAAC CACTGCTGGG TTTCAAGACA TTCTGACCGA GGTGGAAATT TTAGCGGTGA 
     3121 TTGTGGGATG CCTGTGTCAT GACCTCGACC ACAGGGGAAC CAACAATGCC TTCCAAGCTA 
     3181 AGAGTGGCTC TGCCCTGGCC CAACTCTATG GAACCTCTGC TACCTTGGAG CATCACCATT 
     3241 TCAACCACGC CGTGATGATC CTTCAAAGTG AGGGTCACAA TATCTTTGCT AACCTGTCCT 
     3301 CCAAGGAATA TAGTGACCTT ATGCAGCTTT TGAAGCAGTC AATATTGGCA ACAGACCTCA 
     3361 CGCTGTACTT TGAGAGGAGA ACTGAATTCT TTGAACTTGT CAGTAAAGGA GAATACGATT 
     3421 GGAACATCAA AAACCATCGT GATATATTTC GATCAATGTT AATGACAGCC TGTGACCTTG 
     3481 GAGCCGTGAC CAAACCGTGG GAGATCTCCA GACAGGTGGC AGAACTTGTA ACCAGTGAGT 
     3541 TCTTCGAACA AGGAGATCGG GAGAGATTAG AGCTCAAACT CACTCCTTCA GCAATTTTTG 
     3601 ATCGGAACCG GAAGGATGAA CTGCCTCGGT TGCAACTGGA GTGGATTGAT AGCATCTGCA 
     3661 TGCCTTTGTA TCAGGCACTG GTGAAGGTCA ACGTGAAACT GAAGCCGATG CTAGATTCAG 
     3721 TAGCTACAAA CAGAAGTAAG TGGGAAGAGC TACACCAAAA ACGACTGCTG GCCTCAACTG 
     3781 CCTCATCCTC CTCCCCTGCC AGTGTTATGG TAGCCAAGGA AGACAGGAAC ACGCGTACGC 
     3841 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3901 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3961 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     4021 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     4081 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4141 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4201 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4261 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4321 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4381 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4441 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4501 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5281 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5341 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5401 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5461 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5521 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5581 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5641 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5701 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5761 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5821 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5881 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5941 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     6001 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     6061 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6121 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6181 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6241 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6301 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6361 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6421 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6481 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6541 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6601 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6661 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6721 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6781 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6841 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6901 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6961 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     7021 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     7081 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7141 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7201 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7261 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7321 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7381 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7441 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7501 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7561 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7621 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7681 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7741 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7801 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7861 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7921 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7981 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     8041 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     8101 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8161 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8221 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8281 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8341 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8401 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8461 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8521 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8581 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8641 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8701 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8761 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8821 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8881 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8941 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     9001 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     9061 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9121 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9181 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9241 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9301 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9361 TT
//