LOCUS       RG220071                9293 bp    DNA     circular  SYN 
DEFINITION  LIG4 (tGFP-tagged) - Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 2
ACCESSION   NM_206937
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3774..4478 
                     /label="GFP"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    7202..7847 
                     /label="ColE1"  
     misc_feature    5564..6358 
                     /label="Neo"  
     misc_feature    5195..5497 
                     /label="SV40 ori"  
     misc_feature    7862..8722 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3759 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="LIG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCTCA CAAACTTCAC AAACTGTTGC ATCTCACGTT CCTTTTGCAG 
     1081 ATTTGTGTTC AACTTTAGAA CGAATACAGA AAAGTAAAGG ACGTGCAGAA AAAATCAGAC 
     1141 ACTTCAGGGA ATTTTTAGAT TCTTGGAGAA AATTTCATGA TGCTCTTCAT AAGAACCACA 
     1201 AAGATGTCAC AGACTCTTTT TATCCAGCAA TGAGACTAAT TCTTCCTCAG CTAGAAAGAG 
     1261 AGAGAATGGC CTATGGAATT AAAGAAACTA TGCTTGCTAA GCTTTATATT GAGTTGCTTA 
     1321 ATTTACCTAG AGATGGAAAA GATGCCCTCA AACTTTTAAA CTACAGAACA CCCACTGGAA 
     1381 CTCATGGAGA TGCTGGAGAC TTTGCAATGA TTGCATATTT TGTGTTGAAG CCAAGATGTT 
     1441 TACAGAAAGG AAGTTTAACC ATACAGCAAG TAAACGACCT TTTAGACTCA ATTGCCAGCA 
     1501 ATAATTCTGC TAAAAGAAAA GACCTAATAA AAAAGAGCCT TCTTCAACTT ATAACTCAGA 
     1561 GTTCAGCACT TGAGCAAAAG TGGCTTATAC GGATGATCAT AAAGGATTTA AAGCTTGGTG 
     1621 TTAGTCAGCA AACTATCTTT TCTGTTTTTC ATAATGATGC TGCTGAGTTG CATAATGTCA 
     1681 CTACAGATCT GGAAAAAGTC TGTAGGCAAC TGCATGATCC TTCTGTAGGA CTCAGTGATA 
     1741 TTTCTATCAC TTTATTTTCT GCATTTAAAC CAATGCTAGC TGCTATTGCA GATATTGAGC 
     1801 ACATTGAGAA GGATATGAAA CATCAGAGTT TCTACATAGA AACCAAGCTA GATGGTGAAC 
     1861 GTATGCAAAT GCACAAAGAT GGAGATGTAT ATAAATACTT CTCTCGAAAT GGATATAACT 
     1921 ACACTGATCA GTTTGGTGCT TCTCCTACTG AAGGTTCTCT TACCCCATTC ATTCATAATG 
     1981 CATTCAAAGC AGATATACAA ATCTGTATTC TTGATGGTGA GATGATGGCC TATAATCCTA 
     2041 ATACACAAAC TTTCATGCAA AAGGGAACTA AGTTTGATAT TAAAAGAATG GTAGAGGATT 
     2101 CTGATCTGCA AACTTGTTAT TGTGTTTTTG ATGTATTGAT GGTTAATAAT AAAAAGCTAG 
     2161 GGCATGAGAC TCTGAGAAAG AGGTATGAGA TTCTTAGTAG TATTTTTACA CCAATTCCAG 
     2221 GTAGAATAGA AATAGTGCAG AAAACACAAG CTCATACTAA GAATGAAGTA ATTGATGCAT 
     2281 TGAATGAAGC AATAGATAAA AGAGAAGAGG GAATTATGGT AAAACAACCT CTATCCATCT 
     2341 ACAAGCCAGA CAAAAGAGGT GAAGGGTGGT TAAAAATTAA ACCAGAGTAT GTCAGTGGAC 
     2401 TAATGGATGA ATTGGACATT TTAATTGTTG GAGGATATTG GGGTAAAGGA TCACGGGGTG 
     2461 GAATGATGTC TCATTTTCTG TGTGCAGTAG CAGAGAAGCC CCCTCCTGGT GAGAAGCCAT 
     2521 CTGTGTTTCA TACTCTCTCT CGTGTTGGGT CTGGCTGCAC CATGAAAGAA CTGTATGATC 
     2581 TGGGTTTGAA ATTGGCCAAG TATTGGAAGC CTTTTCATAG AAAAGCTCCA CCAAGCAGCA 
     2641 TTTTATGTGG AACAGAGAAG CCAGAAGTAT ACATTGAACC TTGTAATTCT GTCATTGTTC 
     2701 AGATTAAAGC AGCAGAGATC GTACCCAGTG ATATGTATAA AACTGGCTGC ACCTTGCGTT 
     2761 TTCCACGAAT TGAAAAGATA AGAGATGACA AGGAGTGGCA TGAGTGCATG ACCCTGGACG 
     2821 ACCTAGAACA ACTTAGGGGG AAGGCATCTG GTAAGCTCGC ATCTAAACAC CTTTATATAG 
     2881 GTGGTGATGA TGAACCACAA GAAAAAAAGC GGAAAGCTGC CCCAAAGATG AAGAAAGTTA 
     2941 TTGGAATTAT TGAGCACTTA AAAGCACCTA ACCTTACTAA CGTTAACAAA ATTTCTAATA 
     3001 TATTTGAAGA TGTAGAGTTT TGTGTTATGA GTGGAACAGA TAGCCAGCCA AAGCCTGACC 
     3061 TGGAGAACAG AATTGCAGAA TTTGGTGGTT ATATAGTACA AAATCCAGGC CCAGACACGT 
     3121 ACTGTGTAAT TGCAGGGTCT GAGAACATCA GAGTGAAAAA CATAATTTTG TCAAATAAAC 
     3181 ATGATGTTGT CAAGCCTGCA TGGCTTTTAG AATGTTTTAA GACCAAAAGC TTTGTACCAT 
     3241 GGCAGCCTCG CTTTATGATT CATATGTGCC CATCAACCAA AGAACATTTT GCCCGTGAAT 
     3301 ATGATTGCTA TGGTGATAGT TATTTCATTG ATACAGACTT GAACCAACTG AAGGAAGTAT 
     3361 TCTCAGGAAT TAAAAATTCT AACGAGCAGA CTCCTGAAGA AATGGCTTCT CTGATTGCTG 
     3421 ATTTAGAATA TCGGTATTCC TGGGATTGCT CTCCTCTCAG TATGTTTCGA CGCCACACCG 
     3481 TTTATTTGGA CTCGTATGCT GTTATTAATG ACCTGAGTAC CAAAAATGAG GGGACAAGGT 
     3541 TAGCTATTAA AGCCTTGGAG CTTCGGTTTC ATGGAGCAAA AGTAGTTTCT TGTTTAGCTG 
     3601 AGGGAGTGTC TCATGTAATA ATTGGGGAAG ATCATAGTCG TGTTGCAGAT TTTAAAGCTT 
     3661 TTAGAAGAAC TTTTAAGAGA AAGTTTAAAA TCCTAAAAGA AAGTTGGGTA ACTGATTCAA 
     3721 TAGACAAGTG TGAATTACAA GAAGAAAACC AGTATTTGAT TACGCGTACG CGGCCGCTCG 
     3781 AGATGGAGAG CGACGAGAGC GGCCTGCCCG CCATGGAGAT CGAGTGCCGC ATCACCGGCA 
     3841 CCCTGAACGG CGTGGAGTTC GAGCTGGTGG GCGGCGGAGA GGGCACCCCC GAGCAGGGCC 
     3901 GCATGACCAA CAAGATGAAG AGCACCAAAG GCGCCCTGAC CTTCAGCCCC TACCTGCTGA 
     3961 GCCACGTGAT GGGCTACGGC TTCTACCACT TCGGCACCTA CCCCAGCGGC TACGAGAACC 
     4021 CCTTCCTGCA CGCCATCAAC AACGGCGGCT ACACCAACAC CCGCATCGAG AAGTACGAGG 
     4081 ACGGCGGCGT GCTGCACGTG AGCTTCAGCT ACCGCTACGA GGCCGGCCGC GTGATCGGCG 
     4141 ACTTCAAGGT GATGGGCACC GGCTTCCCCG AGGACAGCGT GATCTTCACC GACAAGATCA 
     4201 TCCGCAGCAA CGCCACCGTG GAGCACCTGC ACCCCATGGG CGATAACGAT CTGGATGGCA 
     4261 GCTTCACCCG CACCTTCAGC CTGCGCGACG GCGGCTACTA CAGCTCCGTG GTGGACAGCC 
     4321 ACATGCACTT CAAGAGCGCC ATCCACCCCA GCATCCTGCA GAACGGGGGC CCCATGTTCG 
     4381 CCTTCCGCCG CGTGGAGGAG GATCACAGCA ACACCGAGCT GGGCATCGTG GAGTACCAGC 
     4441 ACGCCTTCAA GACCCCGGAT GCAGATGCCG GTGAAGAAAG AGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCTTGGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5221 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA 
     5281 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC 
     5341 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC 
     5401 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 
     5461 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA 
     5521 GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT 
     5581 GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT 
     5641 GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG 
     5701 GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC 
     5761 GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC 
     5821 GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC 
     5881 CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG 
     5941 CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG 
     6001 CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT 
     6061 CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG 
     6121 GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC 
     6181 TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCCG ACCGCTATCA GGACATAGCG 
     6241 TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG 
     6301 CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG 
     6361 TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 
     6421 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 
     6481 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 
     6541 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 
     6601 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 
     6661 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 
     6721 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 
     6781 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 
     6841 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 
     6901 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 
     6961 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 
     7021 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 
     7081 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 
     7141 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 
     7201 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 
     7261 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 
     7321 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 
     7381 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 
     7441 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 
     7501 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 
     7561 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 
     7621 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     7681 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     7741 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     7801 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 
     7861 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 
     7921 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 
     7981 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 
     8041 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 
     8101 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 
     8161 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 
     8221 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 
     8281 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 
     8341 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 
     8401 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 
     8461 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 
     8521 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 
     8581 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 
     8641 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 
     8701 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 
     8761 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     8821 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     8881 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     8941 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     9001 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     9061 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     9121 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     9181 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     9241 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//