LOCUS       RG219985                8740 bp    DNA     circular  SYN 
DEFINITION  GPD2 (tGFP-tagged) - Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 2
ACCESSION   NM_000408
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3221..3925 
                     /label="GFP"  
     misc_feature    3972..4557 
                     /label="PolyA signal"  
     misc_feature    6649..7294 
                     /label="ColE1"  
     misc_feature    5011..5805 
                     /label="Neo"  
     misc_feature    4642..4944 
                     /label="SV40 ori"  
     misc_feature    7309..8169 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3200..3206 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3200 
                     /label="GPD2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATTTCAA AAGGCAGTGA AAGGGACGAT TCTTGTTGGA GGAGGTGCTC 
     1081 TTGCAACTGT TTTAGGACTT TCTCAGTTTG CTCATTACAG AAGGAAACAA ATGAACCTGG 
     1141 CCTATGTTAA AGCAGCAGAC TGCATTTCAG AACCAGTTAA CAGGGAGCCT CCTTCCAGAG 
     1201 AAGCTCAGCT ACTGACTTTG CAAAACACAT CTGAATTTGA TATCCTTGTT ATTGGAGGAG 
     1261 GAGCAACAGG AAGTGGCTGT GCGCTAGATG CTGTCACCAG AGGACTAAAA ACAGCCCTTG 
     1321 TAGAAAGAGA TGATTTCTCA TCAGGGACCA GCAGCAGAAG CACTAAATTG ATCCATGGTG 
     1381 GTGTGAGATA TCTGCAGAAG GCCATCATGA AGTTGGATAT TGAGCAGTAT AGGATGGTAA 
     1441 AAGAAGCCCT TCATGAGCGT GCCAACCTGC TAGAAATTGC TCCCCATTTA TCAGCTCCAT 
     1501 TGCCTATAAT GCTTCCAGTT TACAAGTGGT GGCAGTTACC TTACTACTGG GTAGGAATCA 
     1561 AGCTGTATGA TTTGGTTGCA GGAAGCAATT GCCTAAAAAG CAGTTATGTC CTCAGCAAAT 
     1621 CAAGAGCCCT TGAACATTTC CCAATGCTCC AGAAGGACAA ACTGGTAGGA GCAATTGTCT 
     1681 ACTATGACGG ACAACATAAC GATGCACGGA TGAACCTTGC CATTGCTCTG ACTGCTGCCA 
     1741 GGTATGGGGC TGCCACAGCC AATTACATGG AGGTAGTGAG CTTGCTCAAG AAGACAGACC 
     1801 CCCAGACAGG GAAAGTGCGT GTGAGCGGCG CACGGTGCAA GGATGTCCTC ACAGGGCAGG 
     1861 AATTTGACGT GAGAGCCAAA TGTGTTATCA ATGCCACGGG ACCTTTCACG GACTCTGTGC 
     1921 GCAAAATGGA TGATAAAGAC GCAGCAGCTA TCTGCCAGCC AAGTGCTGGT GTCCATATTG 
     1981 TGATGCCTGG TTATTACAGC CCAGAGAGCA TGGGACTTCT TGACCCAGCG ACCAGTGATG 
     2041 GGCGAGTTAT TTTCTTCTTA CCCTGGCAAA AGATGACGAT CGCTGGCACT ACTGATACTC 
     2101 CAACTGATGT TACACACCAT CCAATTCCTT CAGAAGAAGA TATCAACTTC ATTTTGAATG 
     2161 AAGTGCGTAA TTACCTGAGT TGTGATGTTG AAGTGAGAAG AGGGGATGTC CTGGCAGCAT 
     2221 GGAGTGGAAT CCGTCCTCTT GTTACAGACC CCAAATCTGC AGATACTCAG TCTATCTCCC 
     2281 GAAATCATGT TGTTGATATC AGTGAGAGTG GCCTTATTAC TATAGCAGGT GGAAAGTGGA 
     2341 CAACTTATCG GTCTATGGCA GAAGATACCA TAAATGCTGC TGTCAAAACT CATAATTTAA 
     2401 AAGCAGGACC AAGTAGAACA GTTGGGCTTT TCCTTCAAGG GGGTAAAGAT TGGAGCCCCA 
     2461 CACTCTACAT TAGGCTTGTG CAGGATTATG GACTTGAAAG CGAGGTGGCA CAGCATCTTG 
     2521 CCGCCACCTA TGGTGATAAG GCCTTTGAGG TGGCCAAATG GCAAGTGTGA CTGGCAAAAG 
     2581 GTGGCCTATT GTTGGAGTAC GTCTTGTGTC AGAATTTCCA TATATTGAAG CAGAGGTGAA 
     2641 ATATGGGATT AAGGAGTATG CCTGCACTGC TGTGGATATG ATTTCACGTC GTACTCGCCT 
     2701 GGCCTTTCTA AATGTCCAGG CAGCAGAGGA AGCCCTACCC AGGATTGTTG AACTGATGGG 
     2761 CAGGGAACTG AATTGGGATG ATTATAAGAA GCAGGAACAA CTTGAAACAG CCAGGAAGTT 
     2821 TCTATATTAT GAAATGGGCT ATAAATCTCG ATCAGAACAG TTAACAGATC GCTCTGAAAT 
     2881 TAGCCTACTG CCTTCAGACA TTGACAGGTA TAAGAAGAGA TTTCATAAGT TTGATGCAGA 
     2941 CCAGAAAGGC TTTATTACCA TTGTTGATGT TCAGCGTGTA TTAGAGAGTA TCAATGTCCA 
     3001 AATGGATGAA AATACACTCC ATGAAATTCT AAATGAAGTT GATTTGAATA AAAATGGACA 
     3061 GGTTGAACTC AATGAATTTT TGCAGCTGAT GAGTGCTATT CAAAAAGGAA GGGTATCTGG 
     3121 AAGCCGGCTT GCTATACTAA TGAAAACTGC AGAAGAGAAC CTCGACAGAA GAGTTCCAAT 
     3181 TCCAGTGGAC CGTAGTTGTG GAGGATTGAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA 
     3241 CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT 
     3301 GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA 
     3361 GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG 
     3421 CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC 
     3481 CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT 
     3541 GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT 
     3601 GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC 
     3661 CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC 
     3721 CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA 
     3781 GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT 
     3841 GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC 
     3901 CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT 
     3961 CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG 
     4021 AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT 
     4081 CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC 
     4141 AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG 
     4201 TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC 
     4261 AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT 
     4321 TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG 
     4381 TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT 
     4441 TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA 
     4501 GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT 
     4561 CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT 
     4621 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 
     4681 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 
     4741 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC 
     4801 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA 
     4861 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT 
     4921 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA 
     4981 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT 
     5041 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 
     5101 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 
     5161 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 
     5221 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 
     5281 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 
     5341 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 
     5401 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 
     5461 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 
     5521 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 
     5581 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 
     5641 TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 
     5701 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 
     5761 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG 
     5821 GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA 
     5881 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG 
     5941 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT 
     6001 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT 
     6061 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG 
     6121 TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG 
     6181 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 
     6241 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 
     6301 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 
     6361 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 
     6421 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6481 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6541 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6601 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6661 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6721 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6781 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6841 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6901 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6961 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     7021 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     7081 AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     7141 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     7201 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     7261 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 
     7321 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 
     7381 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 
     7441 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 
     7501 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 
     7561 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 
     7621 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 
     7681 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 
     7741 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 
     7801 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 
     7861 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 
     7921 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 
     7981 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 
     8041 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 
     8101 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 
     8161 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 
     8221 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 
     8281 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC 
     8341 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC 
     8401 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA 
     8461 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT 
     8521 TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT 
     8581 GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA 
     8641 CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT 
     8701 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//