LOCUS       RG219763                9092 bp    DNA     circular  SYN 
DEFINITION  PRICKLE2 (tGFP-tagged) - Human prickle homolog 2 (Drosophila) (PRICKLE2)
ACCESSION   NM_198859
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3573..4277 
                     /label="GFP"  
     misc_feature    4324..4909 
                     /label="PolyA signal"  
     misc_feature    7001..7646 
                     /label="ColE1"  
     misc_feature    5363..6157 
                     /label="Neo"  
     misc_feature    4994..5296 
                     /label="SV40 ori"  
     misc_feature    7661..8521 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3552..3558 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3552 
                     /label="PRICKLE2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGACAGTG ATGCCGCTGG AGATGGAGAA GACCATCAGC AAACTCATGT 
     1081 TTGACTTTCA GAGGAACTCG ACCTCAGATG ATGACTCAGG CTGTGCTTTG GAAGAGTATG 
     1141 CCTGGGTCCC GCCGGGTCTG AAGCCTGAAC AGGTACACCA GTACTATAGC TGTCTCCCAG 
     1201 AAGAGAAAGT CCCTTATGTC AACAGTCCTG GAGAGAAACT GCGAATCAAG CAGCTACTAC 
     1261 ACCAGCTGCC GCCACATGAC AATGAGGTTC GATATTGCAA CTCCCTGGAT GAGGAAGAGA 
     1321 AGAGGGAGCT GAAGCTTTTC AGCAGCCAGA GGAAACGCGA AAACTTGGGC CGCGGGAATG 
     1381 TCAGGCCTTT CCCAGTCACC ATGACAGGAG CTATTTGTGA ACAGTGCGGA GGCCAGATCA 
     1441 ATGGTGGAGA CATCGCTGTG TTTGCGTCAC GCGCTGGCCA CGGCGTTTGC TGGCACCCGC 
     1501 CGTGCTTCGT ATGCACTGTC TGCAATGAGC TCCTGGTGGA TCTGATCTAC TTTTACCAAG 
     1561 ATGGGAAGAT ATACTGTGGC AGGCACCATG CTGAGTGCCT GAAGCCGCGC TGTGCTGCCT 
     1621 GCGATGAGAT CATCTTTGCA GATGAATGCA CAGAAGCTGA GGGGCGACAC TGGCACATGA 
     1681 AACACTTTTG CTGCTTCGAG TGTGAGACAG TGCTGGGCGG CCAGCGCTAC ATCATGAAGG 
     1741 AGGGAAGACC CTACTGTTGC CACTGCTTCG AGTCCTTGTA TGCAGAATAT TGTGACACCT 
     1801 GTGCCCAACA TATAGGTATC GACCAAGGTC AAATGACCTA TGACGGCCAA CACTGGCATG 
     1861 CCACTGAGAC CTGTTTCTGC TGTGCTCACT GCAAGAAATC CCTCCTGGGG CGGCCATTCC 
     1921 TCCCGAAGCA GGGCCAGATA TTCTGCTCAC GGGCCTGCAG TGCTGGGGAA GACCCCAATG 
     1981 GTTCTGACTC CTCTGATTCC GCCTTCCAGA ACGCCAGGGC CAAGGAGTCC CGGCGCAGTG 
     2041 CCAAAATTGG CAAGAACAAG GGCAAGACGG AGGAGCCCAT GCTGAACCAG CACAGCCAGC 
     2101 TGCAAGTGAG TTCTAACCGG CTGTCAGCCG ACGTAGACCC CCTGTCACTG CAGATGGACA 
     2161 TGCTCAGCCT GTCCAGCCAG ACACCCAGCC TCAACCGGGA CCCCATCTGG AGGAGCCGGG 
     2221 AAGAGCCCTA CCATTATGGG AACAAGATGG AGCAGAACCA GACCCAGAGC CCTCTGCAGC 
     2281 TCCTCAGCCA GTGCAACATC AGAACTTCCT ACAGTCCAGG AGGGCAAGGG GCTGGGGCCC 
     2341 AGCCCGAAAT GTGGGGCAAG CACTTCAGCA ACCCCAAAAG GAGCTCGTCA CTGGCCATGA 
     2401 CAGGACATGC TGGCAGCTTC ATCAAGGAAT GCCGAGAAGA CTATTACCCG GGGAGGCTGA 
     2461 GATCTCAGGA GAGCTACAGT GATATGTCTA GTCAGAGTTT CAGTGAGACC CGAGGCAGCA 
     2521 TCCAAGTCCC CAAATATGAG GAGGAAGAGG AAGAGGAAGG GGGCTTGTCC ACTCAGCAGT 
     2581 GTCGGACCCG TCATCCCATC AGTTCCCTGA AATACACAGA GGACATGACG CCCACAGAGC 
     2641 AGACCCCTCG GGGCTCCATG GAATCCCTGG CCCTGTCTAA TGCAACAGGC CTCTCTGCTG 
     2701 ATGGTGGTGC CAAGCGCCAG GAGCACCTAT CCCGATTTTC CATGCCTGAC CTCAGCAAAG 
     2761 ACTCTGGAAT GAATGTGTCT GAGAAGCTGA GCAACATGGG CACTCTTAAC TCGTCCATGC 
     2821 AGTTCCGGAG CGCAGAGTCA GTTCGCAGCC TGCTCTCTGC CCAGCAGTAC CAGGAGATGG 
     2881 AGGGAAACCT CCACCAGCTC AGCAACCCCA TTGGCTACAG AGACCTGCAG TCCCACGGAA 
     2941 GGATGCATCA GAGCTTTGAT TTTGATGGAG GGATGGCGGG CAGCAAGCTG CCAGGGCAGG 
     3001 AGGGCGTGAG GATCCAGCCC ATGAGTGAAC GCACCCGGAG AAGAGCTACT TCACGCGACG 
     3061 ACAACCGCCG TTTCCGACCT CACAGGTCCA GGCGTTCCCG ACGCTCTCGC TCCGACAACG 
     3121 CCCTCCACCT GGCCAGCGAA CGCGAGGCCA TCTCCCGGTT AAAAGATAGG CCCCCTCTGA 
     3181 GAGCCAGGGA GGACTATGAC CAATTTATGC GCCAGCGGAG CTTCCAGGAG AGCATGGGGC 
     3241 ATGGGTCCCG GAGGGACCTG TACGGCCAGT GCCCTAGGAC TGTGTCGGAC CTGGCTTTGC 
     3301 AGAATGCCTT TGGGGACCGC TGGGGACCCT ACTTCGCCGA GTATGATTGG TGTTCCACCT 
     3361 GCTCCTCCTC TTCAGAGTCT GACAACGAGG GCTATTTCCT AGGAGAACCC ATCCCCCAGC 
     3421 CAGCGCGCCT GCGATACGTC ACAAGCGATG AGCTGCTGCA CAAATACAGC TCCTACGGCC 
     3481 TCCCCAAATC TTCCACATTA GGTGGCAGAG GACAGTTGCA CAGCAGGAAA AGACAGAAGA 
     3541 GCAAAAACTG TATCATTTCT ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3601 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3661 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     3721 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     3781 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     3841 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     3901 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     3961 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4021 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4081 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4141 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4201 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4261 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4321 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4381 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4441 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4501 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4561 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4621 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4681 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4741 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4801 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4861 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4921 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     4981 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5041 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5101 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5161 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5221 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5281 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5341 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5401 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5461 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5521 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5581 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5641 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     5701 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     5761 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     5821 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     5881 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     5941 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6001 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6061 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6121 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6181 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6241 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6301 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6361 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6421 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6481 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6541 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6601 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6661 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     6721 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     6781 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     6841 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     6901 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     6961 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7021 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7081 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7141 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7201 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7261 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7321 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7381 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7441 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7501 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7561 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7621 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7681 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     7741 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     7801 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     7861 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     7921 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     7981 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8041 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8101 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8161 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8221 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8281 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8341 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8401 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8461 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8521 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8581 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8641 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     8701 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     8761 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     8821 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     8881 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     8941 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9001 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9061 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//