LOCUS       RG219362                9128 bp    DNA     circular  SYN 
DEFINITION  DDX11 (tGFP-tagged) - Human DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (DDX11), transcript variant 2
ACCESSION   NM_004399
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3609..4313 
                     /label="GFP"  
     misc_feature    4360..4945 
                     /label="PolyA signal"  
     misc_feature    7037..7682 
                     /label="ColE1"  
     misc_feature    5399..6193 
                     /label="Neo"  
     misc_feature    5030..5332 
                     /label="SV40 ori"  
     misc_feature    7697..8557 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3588..3594 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3588 
                     /label="DDX11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAATGAA ACACAGAAGG TTGGTGCCAT CCATTTTCCT TTTCCCTTCA 
     1081 CACCCTATTC CATCCAGGAA GACTTCATGG CAGAGCTGTA CCGGGTTTTG GAGGCTGGCA 
     1141 AGAGTGGGAT ATTTGAGAGT CCAACTGGCA CTGGGAAGTC CTTAAGTCTT ATTTGTGGGG 
     1201 CCCTCTCTTG GCTCCGTGAC TTTGAACAGA AGAAGCGTGA AGAAGAGGCA CGACTCCTTG 
     1261 AAACTGGAAC TGGCCCCTTA CATGATGAGA AAGATGAATC CCTGTGTCTG TCTTCTTCCT 
     1321 GCGAAGGGGC TGCAGGCACC CCGAGGCCTG CTGGAGAACC GGCCTGGGTT ACTCAGTTTG 
     1381 TGCAGAAGAA AGAAGAGAGG GACCTGGTGG ACCGACTAAA GGCGGAGCAG GCCAGGAGGA 
     1441 AGCAGCGAGA AGAACGCCTG CAGCAGCTGC AGCACAGGGT GCAGCTCAAG TATGCAGCCA 
     1501 AGCGCCTGAG GCAGGAAGAA GAAGAAAGAG AGAATCTCCT CCGCCTCAGC AGGGAGATGC 
     1561 TAGAGACAGG CCCGGAGGCT GAGCGGCTGG AGCAGCTGGA GTCTGGGGAG GAGGAGCTGG 
     1621 TCCTCGCCGA ATACGAGAGT GATGAGGAGA AAAAGGTGGC GAGCAGAGTG GATGAGGATG 
     1681 AGGATGACCT GGAGGAAGAA CACATAACTA AGATTTATTA CTGTAGTCGG ACACACTCCC 
     1741 AGCTGGCCCA GTTTGTGCAT GAGGTGAAGA AGAGCCCCTT TGGCAAGGAT GTTCGGCTGG 
     1801 TCTCCCTTGG CTCCCGGCAG AACCTTTGTG TAAATGAAGA CGTGAAAAGC CTAGGTTCTG 
     1861 TGCAGCTTAT CAACGACCGC TGTGTGGACA TGCAGAGAAG CAGGCACGAG AAGAAGAAAG 
     1921 GAGCTGAGGA GGAGAAGCCA AAGAGGAGGA GGCAGGAGAA GCAGGCAGCC TGCCCCTTCT 
     1981 ACAACCACGA GCAGATGGGC CTTCTCCGGG ATGAGGCCCT GGCAGAGGTG AAGGACATGG 
     2041 AGCAGCTGCT GGCCCTTGGG AAGGAGGCCC GGGCCTGTCC CTATTACGGG AGCCGCCTTG 
     2101 CCATCCCTGC AGCCCAGCTG GTGGTGCTGC CCTATCAGAT GCTGCTGCAT GCGGCCACTC 
     2161 GGCAGGCCGC GGGCATCCGG CTGCAGGACC AGGTGGTGAT CATCGACGAG GCGCACAACC 
     2221 TGATCGACAC CATCACGGGC ATGCACAGCG TGGAGGTCAG CGGCTCCCAG CTCTGCCAGG 
     2281 CCCATTCCCA GCTGCTGCAG TACGTGGAGC GATACGGGAA GCGTTTGAAG GCCAAGAACC 
     2341 TGATGTACCT GAAGCAGATC CTGTATTTGC TGGAGAAATT CGTGGCTGTG CTAGGGGGGA 
     2401 ACATTAAGCA AAATCCCAAT ACACAGAGTC TGTCACAGAC AGGGACGGAG CTGAAGACCA 
     2461 TCAACGACTT TCTCTTCCAG AGCCAGATCG ACAACATCAA CCTGTTCAAG GTGCAGCGAT 
     2521 ACTGTGAGAA GAGCATGATC AGCAGAAAGC TCTTTGGATT CACTGAACGG TACGGAGCAG 
     2581 TGTTCTCATC CCGGGAGCAG CCCAAACTGG CTGGGTTTCA GCAATTCCTG CAGAGCCTGC 
     2641 AGCCCAGGAC GACTGAAGCT CTTGCAGCCC CTGCAGACGA GAGTCAGGCC AGCACCCTGC 
     2701 GACCAGCTTC TCCACTGATG CACATCCAAG GCTTCCTGGC AGCTCTCACT ACGGCCAACC 
     2761 AGGACGGCAG GGTCATCCTG AGCCGCCAAG GCAGCCTCAG TCAGAGCACC CTGAAGTTTT 
     2821 TGCTCCTGAA TCCAGCTGTG CACTTTGCCC AAGTGGTGAA GGAATGCCGG GCAGTGGTCA 
     2881 TTGCGGGGGG TACCATGCAG CCGGTGTCTG ACTTCCGGCA GCAGCTGCTG GCCTGTGCCG 
     2941 GGGTGGAAGC TGAGCGCGGT GGTGGAGTTT TCTGTGGTCA CGTGATCCCT CCAGACAACA 
     3001 TCCTGCCCCT CGTCATCTGC AGCGGGATCT CCAACCAGCC GCTGGAATTC ACGTTCCAGA 
     3061 AAAGAGAGCT GCCTCAGATG ATATTCCAGG AACCTAAGAG CGCACACCAG GTGGAGCAGG 
     3121 TGCTGCTGGC ATATTCCAGG TGCATCCAGG CCTGTGGCCA GGAGAGAGGC CAGGTGACAG 
     3181 GGGCCCTGCT CCTCTCTGTG GTTGGAGGAA AGATGAGTGA AGGGATCAAC TTCTCTGACA 
     3241 ACCTAGGCCG GTGTGTGGTG ATGGTGGGCA TGCCCTTCCC CAACATCAGG TCTGCAGAGC 
     3301 TGCAGGAGAA GATGGCCTAC TTGGATCAAA CCCTCCCCAG AGCCCCCGGC CAGGCACCCC 
     3361 CAGGGAAGGC TCTGGTGGAG AACCTGTGCA TGAAGGCCGT CAACCAGTCC ATAGGCAGGG 
     3421 CCATCAGGCA CCAGAAGGAT TTTGCCAGCG TAGTGCTCCT GGACCAGCGA TATGCCCGGC 
     3481 CCCCTGTCCT GGCCAAGCTG CCGGCCTGGA TCCGAGCCCG TGTGGAGGTC AAAGCTACCT 
     3541 TTGGCCCCGC CATTGCTGCT GTGCAGAAGT TTCACCGGGA GAAGTCGGCC TCTTCCACGC 
     3601 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3661 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3721 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3781 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3841 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     3901 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     3961 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4021 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4081 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4141 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4201 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4261 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5041 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5101 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5161 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5221 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5281 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5341 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5401 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5461 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5521 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5581 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5641 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5701 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5761 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5821 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     5881 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     5941 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6001 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6061 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6121 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6181 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6241 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6301 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6361 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6421 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6481 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6541 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6601 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6661 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6721 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6781 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6841 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     6901 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     6961 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7021 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7081 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7141 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7201 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7261 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7321 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7381 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7441 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7501 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7561 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7621 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7681 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7741 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7801 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7861 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     7921 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     7981 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8041 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8101 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8161 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8221 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8281 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8341 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8401 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8461 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8521 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8581 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8641 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8701 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8761 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8821 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     8881 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     8941 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9001 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9061 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9121 CGAATTTT
//