LOCUS       RG219286                9083 bp    DNA     circular  SYN 
DEFINITION  TAS1R1 (tGFP-tagged) - Human taste receptor, type 1, member 1 (TAS1R1), transcript variant 2
ACCESSION   NM_138697
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3564..4268 
                     /label="GFP"  
     misc_feature    4315..4900 
                     /label="PolyA signal"  
     misc_feature    6992..7637 
                     /label="ColE1"  
     misc_feature    5354..6148 
                     /label="Neo"  
     misc_feature    4985..5287 
                     /label="SV40 ori"  
     misc_feature    7652..8512 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3543..3549 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3543 
                     /label="TAS1R1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGCTCTGC ACGGCTCGCC TGGTCGGCCT GCAGCTTCTC ATTTCCTGCT 
     1081 GCTGGGCCTT TGCCTGCCAT AGCACGGAGT CTTCTCCTGA CTTCACCCTC CCCGGAGATT 
     1141 ACCTCCTGGC AGGCCTGTTC CCTCTCCATT CTGGCTGTCT GCAGGTGAGG CACAGACCCG 
     1201 AGGTGACCCT GTGTGACAGG TCTTGTAGCT TCAATGAGCA TGGCTACCAC CTCTTCCAGG 
     1261 CTATGCGGCT TGGGGTTGAG GAGATAAACA ACTCCACGGC CCTGCTGCCC AACATCACCC 
     1321 TGGGGTACCA GCTGTATGAT GTGTGTTCTG ACTCTGCCAA TGTGTATGCC ACGCTGAGAG 
     1381 TGCTCTCCCT GCCAGGGCAA CACCACATAG AGCTCCAAGG AGACCTTCTC CACTATTCCC 
     1441 CTACGGTGCT GGCAGTGATT GGGCCTGACA GCACCAACCG TGCTGCCACC ACAGCCGCCC 
     1501 TGCTGAGCCC TTTCCTGGTG CCCATGATTA GCTATGCGGC CAGCAGCGAG ACGCTCAGCG 
     1561 TGAAGCGGCA GTATCCCTCT TTCCTGCGCA CCATCCCCAA TGACAAGTAC CAGGTGGAGA 
     1621 CCATGGTGCT GCTGCTGCAG AAGTTCGGGT GGACCTGGAT CTCTCTGGTT GGCAGCAGTG 
     1681 ACGACTATGG GCAGCTAGGG GTGCAGGCAC TGGAGAACCA GGCCACTGGT CAGGGGATCT 
     1741 GCATTGCTTT CAAGGACATC ATGCCCTTCT CTGCCCAGGT GGGCGATGAG AGGATGCAGT 
     1801 GCCTCATGCG CCACCTGGCC CAGGCCGGGG CCACCGTCGT GGTTGTTTTT TCCAGCCGGC 
     1861 AGTTGGCCAG GGTGTTTTTC GAGTCCGTGG TGCTGACCAA CCTGACTGGC AAGGTGTGGG 
     1921 TCGCCTCAGA AGCCTGGGCC CTCTCCAGGC ACATCACTGG GGTGCCCGGG ATCCAGCGCA 
     1981 TTGGGATGGT GCTGGGCGTG GCCATCCAGA AGAGGGCTGT CCCTGGCCTG AAGGCGTTTG 
     2041 AAGAAGCCTA TGCCCGGGCA GACAAGAAGG CCCCTAGGCC TTGCCACAAG GGCTCCTGGT 
     2101 GCAGCAGCAA TCAGCTCTGC AGAGAATGCC AAGCTTTCAT GGCACACACG ATGCCCAAGC 
     2161 TCAAAGCCTT CTCCATGAGT TCTGCCTACA ACGCATACCG GGCTGTGTAT GCGGTGGCCC 
     2221 ATGGCCTCCA CCAGCTCCTG GGCTGTGCCT CTGGAGCTTG TTCCAGGGGC CGAGTCTACC 
     2281 CCTGGCAGCT TTTGGAGCAG ATCCACAAGG TGCATTTCCT TCTACACAAG GACACTGTGG 
     2341 CGTTTAATGA CAACAGAGAT CCCCTCAGTA GCTATAACAT AATTGCCTGG GACTGGAATG 
     2401 GACCCAAGTG GACCTTCACG GTCCTCGGTT CCTCCACATG GTCTCCAGTT CAGCTAAACA 
     2461 TAAATGAGAC CAAAATCCAG TGGCACGGAA AGGACAACCA GGTGCCTAAG TCTGTGTGTT 
     2521 CCAGCGACTG TCTTGAAGGG CACCAGCGAG TGGTTACGGG TTTCCATCAC TGCTGCTTTG 
     2581 AGTGTGTGCC CTGTGGGGCT GGGACCTTCC TCAACAAGAG TGACCTCTAC AGATGCCAGC 
     2641 CTTGTGGGAA AGAAGAGTGG GCACCTGAGG GAAGCCAGAC CTGCTTCCCG CGCACTGTGG 
     2701 TGTTTTTGGC TTTGCGTGAG CACACCTCTT GGGTGCTGCT GGCAGCTAAC ACGCTGCTGC 
     2761 TGCTGCTGCT GCTTGGGACT GCTGGCCTGT TTGCCTGGCA CCTAGACACC CCTGTGGTGA 
     2821 GGTCAGCAGG GGGCCGCCTG TGCTTTCTTA TGCTGGGCTC CCTGGCAGCA GGTAGTGGCA 
     2881 GCCTCTATGG CTTCTTTGGG GAACCCACAA GGCCTGCGTG CTTGCTACGC CAGGCCCTCT 
     2941 TTGCCCTTGG TTTCACCATC TTCCTGTCCT GCCTGACAGT TCGCTCATTC CAACTAATCA 
     3001 TCATCTTCAA GTTTTCCACC AAGGTACCTA CATTCTACCA CGCCTGGGTC CAAAACCACG 
     3061 GTGCTGGCCT GTTTGTGATG ATCAGCTCAG CGGCCCAGCT GCTTATCTGT CTAACTTGGC 
     3121 TGGTGGTGTG GACCCCACTG CCTGCTAGGG AATACCAGCG CTTCCCCCAT CTGGTGATGC 
     3181 TTGAGTGCAC AGAGACCAAC TCCCTGGGCT TCATACTGGC CTTCCTCTAC AATGGCCTCC 
     3241 TCTCCATCAG TGCCTTTGCC TGCAGCTACC TGGGTAAGGA CTTGCCAGAG AACTACAACG 
     3301 AGGCCAAATG TGTCACCTTC AGCCTGCTCT TCAACTTCGT GTCCTGGATC GCCTTCTTCA 
     3361 CCACGGCCAG CGTCTACGAC GGCAAGTACC TGCCTGCGGC CAACATGATG GCTGGGCTGA 
     3421 GCAGCCTGAG CAGCGGCTTC GGTGGGTATT TTCTGCCTAA GTGCTACGTG ATCCTCTGCC 
     3481 GCCCAGACCT CAACAGCACA GAGCACTTCC AGGCCTCCAT TCAGGACTAC ACGAGGCGCT 
     3541 GCGGCTCCAC CACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3601 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3661 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3721 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     3781 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     3841 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     3901 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     3961 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4021 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4081 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4141 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4201 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4261 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4321 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4381 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4441 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4501 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4561 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4621 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4681 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4741 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4801 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4861 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4921 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     4981 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5041 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5101 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5161 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5221 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5281 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5341 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5401 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5461 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5521 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5581 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5641 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5701 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     5761 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     5821 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     5881 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     5941 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6001 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6061 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6121 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6181 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6241 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6301 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6361 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6421 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6481 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6541 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6601 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6661 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6721 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     6781 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     6841 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     6901 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     6961 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7021 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7081 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7141 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7201 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7261 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7321 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7381 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7441 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7501 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7561 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7621 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7681 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7741 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     7801 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     7861 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     7921 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     7981 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8041 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8101 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8161 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8221 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8281 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8341 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8401 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8461 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8521 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8581 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8641 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8701 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     8761 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     8821 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     8881 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     8941 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9001 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9061 AACAAAAATT TAACGCGAAT TTT
//