LOCUS       RG218899                9392 bp    DNA     circular  SYN 
DEFINITION  GANAB (tGFP-tagged) - Human glucosidase, alpha; neutral AB (GANAB), transcript variant 2
ACCESSION   NM_198334
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3873..4577 
                     /label="GFP"  
     misc_feature    4624..5209 
                     /label="PolyA signal"  
     misc_feature    7301..7946 
                     /label="ColE1"  
     misc_feature    5663..6457 
                     /label="Neo"  
     misc_feature    5294..5596 
                     /label="SV40 ori"  
     misc_feature    7961..8821 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3852..3858 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3852 
                     /label="GANAB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGTA GCGGCAGTGG CGGCGCGTAG GAGGCGGTCT TGGGCGTCTT 
     1081 TGGTACTGGC TTTTTTAGGG GTCTGCCTGG GGATTACCCT TGCTGTGGAT AGAAGCAACT 
     1141 TTAAGACCTG TGAAGAGAGT TCTTTCTGCA AGCGACAGAG AAGCATACGG CCAGGCCTCT 
     1201 CTCCATACCG AGCCTTGCTG GACTCTCTAC AGCTTGGTCC TGATTCCCTC ACGGTCCATC 
     1261 TGATCCATGA GGTCACCAAG GTGTTGCTGG TGCTAGAGCT TCAGGGGCTT CAAAAGAACA 
     1321 TGACTCGGTT CAGGATTGAT GAGCTGGAGC CTCGGCGACC CCGATACCGT GTACCAGATG 
     1381 TTTTGGTGGC TGATCCACCA ATAGCCCGGC TTTCTGTCTC TGGTCGTGAT GAGAACAGTG 
     1441 TGGAGTTAAC CATGGCTGAG GGACCCTACA AGATCATCTT GACAGCACGG CCATTCCGCC 
     1501 TTGACCTACT AGAGGACCGA AGTCTTTTGC TTAGTGTCAA TGCCCGAGGA CTCTTGGAGT 
     1561 TTGAGCATCA GAGGGCCCCT AGGGTCTCGC AAGGATCAAA AGACCCAGCT GAGGGCGATG 
     1621 GGGCCCAGCC TGAGGAAACA CCCAGGGATG GCGACAAGCC AGAGGAGACT CAGGGGAAGG 
     1681 CAGAGAAAGA TGAGCCAGGA GCCTGGGAGG AGACATTCAA AACTCACTCT GACAGCAAGC 
     1741 CGTATGGCCC CATGTCTGTG GGTTTGGACT TCTCTCTGCC AGGCATGGAG CATGTCTATG 
     1801 GGATCCCTGA GCATGCAGAC AACCTGAGGC TGAAGGTCAC TGAGGGTGGG GAGCCATATC 
     1861 GCCTCTACAA TTTGGATGTG TTCCAGTATG AGCTGTACAA CCCAATGGCC TTGTATGGGT 
     1921 CTGTGCCTGT GCTCCTGGCA CACAACCCTC ATCGCGACTT GGGCATCTTC TGGCTCAATG 
     1981 CTGCAGAGAC CTGGGTTGAT ATATCTTCCA ACACTGCCGG GAAGACCCTG TTTGGGAAGA 
     2041 TGATGGACTA CCTGCAGGGC TCTGGGGAGA CCCCACAGAC AGATGTTCGC TGGATGTCAG 
     2101 AGACTGGCAT CATTGACGTC TTCCTGCTGC TGGGGCCCTC CATCTCTGAT GTTTTCCGGC 
     2161 AATATGCTAG TCTCACAGGA ACCCAGGCGT TGCCCCCACT CTTCTCCCTC GGCTACCACC 
     2221 AGAGCCGTTG GAACTACCGG GACGAGGCTG ATGTGCTGGA AGTGGATCAG GGCTTTGATG 
     2281 ATCACAACCT GCCCTGTGAT GTCATCTGGC TAGACATTGA ACATGCTGAT GGCAAGCGGT 
     2341 ATTTCACCTG GGACCCCAGT CGCTTCCCTC AGCCCCGCAC CATGCTTGAG CGCTTGGCTT 
     2401 CTAAGAGGCG GAAGCTGGTG GCCATCGTAG ACCCCCACAT CAAGGTGGAC TCCGGCTACC 
     2461 GAGTTCACGA GGAGCTGCGG AACCTGGGGC TGTATGTTAA AACCCGGGAT GGCTCTGACT 
     2521 ATGAGGGCTG GTGCTGGCCA GGCTCAGCTG GTTACCCTGA CTTCACTAAT CCCACGATGA 
     2581 GGGCCTGGTG GGCTAACATG TTCAGCTATG ACAATTATGA GGGCTCAGCT CCCAACCTCT 
     2641 TTGTCTGGAA TGACATGAAC GAACCATCTG TGTTCAATGG TCCTGAGGTC ACCATGCTCA 
     2701 AGGATGCCCA GCATTATGGG GGCTGGGAGC ACCGGGATGT GCATAACATC TATGGCCTTT 
     2761 ATGTGCACAT GGCGACTGCT GATGGGCTGA GACAGCGCTC TGGGGGCATG GAACGCCCCT 
     2821 TTGTCCTGGC CAGGGCCTTC TTCGCTGGCT CCCAGCGCTT TGGAGCCGTG TGGACAGGGG 
     2881 ACAACACTGC CGAGTGGGAC CATTTGAAGA TCTCTATTCC TATGTGTCTC AGCTTGGGGC 
     2941 TGGTGGGACT TTCCTTCTGT GGGGCGGATG TGGGTGGCTT CTTCAAAAAC CCAGAGCCAG 
     3001 AGCTGCTTGT GCGCTGGTAC CAGATGGGTG CTTACCAGCC ATTCTTCCGG GCACATGCCC 
     3061 ACTTGGACAC TGGGCGACGA GAGCCATGGC TGTTACCATC TCAGCACAAT GATATAATCC 
     3121 GAGATGCCTT GGGCCAGCGA TATTCTTTGC TGCCCTTCTG GTACACCCTC TTATATCAGG 
     3181 CCCATCGGGA AGGCATTCCT GTCATGAGGC CCCTGTGGGT GCAGTACCCT CAGGATGTGA 
     3241 CTACCTTCAA TATAGATGAT CAGTACTTGC TTGGGGATGC GTTGCTGGTT CACCCTGTAT 
     3301 CAGACTCTGG AGCCCATGGT GTCCAGGTCT ATCTGCCTGG CCAAGGGGAG GTGTGGTATG 
     3361 ACATTCAAAG CTACCAGAAG CATCATGGTC CCCAGACCCT GTACCTGCCT GTAACTCTAA 
     3421 GCAGTATCCC TGTGTTCCAG CGTGGAGGGA CAATCGTGCC TCGATGGATG CGAGTGCGGC 
     3481 GGTCTTCAGA ATGTATGAAG GATGACCCCA TCACTCTCTT TGTTGCACTT AGCCCTCAGG 
     3541 GTACAGCTCA AGGAGAGCTC TTTCTGGATG ATGGGCACAC GTTCAACTAT CAGACTCGCC 
     3601 AAGAGTTCCT GCTGCGTCGA TTCTCATTCT CTGGCAACAC CCTTGTCTCC AGCTCAGCAG 
     3661 ACCCTGAAGG ACACTTTGAG ACACCAATCT GGATTGAGCG GGTGGTGATA ATAGGGGCTG 
     3721 GAAAGCCAGC AGCTGTGGTA CTCCAGACAA AAGGATCTCC AGAAAGCCGC CTGTCCTTCC 
     3781 AGCATGACCC TGAGACCTCT GTGTTGGTCC TGCGCAAGCC TGGCATCAAT GTGGCATCTG 
     3841 ATTGGAGTAT TCACCTGCGA ACGCGTACGC GGCCGCTCGA GATGGAGAGC GACGAGAGCG 
     3901 GCCTGCCCGC CATGGAGATC GAGTGCCGCA TCACCGGCAC CCTGAACGGC GTGGAGTTCG 
     3961 AGCTGGTGGG CGGCGGAGAG GGCACCCCCG AGCAGGGCCG CATGACCAAC AAGATGAAGA 
     4021 GCACCAAAGG CGCCCTGACC TTCAGCCCCT ACCTGCTGAG CCACGTGATG GGCTACGGCT 
     4081 TCTACCACTT CGGCACCTAC CCCAGCGGCT ACGAGAACCC CTTCCTGCAC GCCATCAACA 
     4141 ACGGCGGCTA CACCAACACC CGCATCGAGA AGTACGAGGA CGGCGGCGTG CTGCACGTGA 
     4201 GCTTCAGCTA CCGCTACGAG GCCGGCCGCG TGATCGGCGA CTTCAAGGTG ATGGGCACCG 
     4261 GCTTCCCCGA GGACAGCGTG ATCTTCACCG ACAAGATCAT CCGCAGCAAC GCCACCGTGG 
     4321 AGCACCTGCA CCCCATGGGC GATAACGATC TGGATGGCAG CTTCACCCGC ACCTTCAGCC 
     4381 TGCGCGACGG CGGCTACTAC AGCTCCGTGG TGGACAGCCA CATGCACTTC AAGAGCGCCA 
     4441 TCCACCCCAG CATCCTGCAG AACGGGGGCC CCATGTTCGC CTTCCGCCGC GTGGAGGAGG 
     4501 ATCACAGCAA CACCGAGCTG GGCATCGTGG AGTACCAGCA CGCCTTCAAG ACCCCGGATG 
     4561 CAGATGCCGG TGAAGAAAGA GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4621 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4681 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4741 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4801 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4861 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4921 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4981 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5041 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5101 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5161 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5221 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCTTGGCT GTGGAATGTG 
     5281 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 
     5341 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5401 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 
     5461 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 
     5521 ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 
     5581 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 
     5641 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 
     5701 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 
     5761 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 
     5821 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 
     5881 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 
     5941 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 
     6001 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 
     6061 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 
     6121 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 
     6181 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 
     6241 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 
     6301 GGCCGGCTGG GTGTGGCCGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 
     6361 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 
     6421 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 
     6481 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 
     6541 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 
     6601 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 
     6661 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 
     6721 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 
     6781 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 
     6841 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 
     6901 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 
     6961 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 
     7021 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 
     7081 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 
     7141 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 
     7201 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 
     7261 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 
     7321 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 
     7381 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 
     7441 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 
     7501 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 
     7561 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 
     7621 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC 
     7681 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 
     7741 CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 
     7801 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 
     7861 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 
     7921 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 
     7981 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 
     8041 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 
     8101 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 
     8161 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 
     8221 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 
     8281 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 
     8341 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 
     8401 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 
     8461 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 
     8521 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 
     8581 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 
     8641 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 
     8701 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 
     8761 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 
     8821 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 
     8881 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 
     8941 TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 
     9001 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 
     9061 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 
     9121 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 
     9181 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 
     9241 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 
     9301 CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG 
     9361 AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//