LOCUS       RG218831                9317 bp    DNA     circular  SYN 
DEFINITION  NBPF11 (tGFP-tagged) - Human neuroblastoma breakpoint family, member 11 (NBPF11). Note: ORF is codon optimized
ACCESSION   NM_183372
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3798..4502 
                     /label="GFP"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    7226..7871 
                     /label="ColE1"  
     misc_feature    5588..6382 
                     /label="Neo"  
     misc_feature    5219..5521 
                     /label="SV40 ori"  
     misc_feature    7886..8746 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3777..3783 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3777 
                     /label="NBPF11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTAAAGCAA AATAATATCA TTCCCGGAGA AACACAAATT CTCCTGAGGT 
     1081 TCACAGGCTG GGAATCAAAG GTGAATGCTA AAAAGCAGCT GCCAGTGGGC ATAAAGTGTG 
     1141 AGCCAATGGA TCAGGTGCCC GATTCAACTT CTAGCGCAAC CAATGTCTCC ATGGTGGTTT 
     1201 CCGCAGGGCC ATGGTCTTCA GAAAAAGCCG AGATGAACAT TCTGGAGATC AACGAAAAGC 
     1261 TGCGCCCGCA GCTGGCTGAG AACAAGCAGC AGTTCCGGAA CCTGAAGGAA CGCTGCTTTC 
     1321 TGACACAGTT GGCGGGGTTC CTTGCTAATA GGCAGAAGAA ATACAAATAC GAAGAGTGCA 
     1381 AGGATCTTAT CAAATTCATG CTGCGGAACG AGCGCCAGTT TAAGGAAGAG AAACTGGCTG 
     1441 AGCAGTTGAA ACAGGCCGAA GAGCTCCGGC AGTACAAGGT TCTCGTGCAC TCTCAGGAGC 
     1501 GCGAGCTGAC CCAGCTGCGA GAGAAACTTA GAGAAGGTCG CGACGCATCA CGATCTCTTA 
     1561 ATGAACATTT GCAGGCACTG CTGACGCCTG ACGAACCCGA TAAGTCACAA GGCCAGGATT 
     1621 TGCAGGAACA GCTTGCCGAG GGCTGCCGAC TGGCCCAACA CCTTGTACAA AAACTGAGCC 
     1681 CCGAGAACGA CGAAGATGAA GACGAGGATG TCCAGGTGGA AGAAGACGAG AAGGTCTTGG 
     1741 AATCTAGTGC CCCTAGGGAA GTCCAAAAGG CCGAAGAGTC TAAGGTACCT GAGGATAGCC 
     1801 TGGAAGAGTG CGCTATCACT TGCAGCAACA GCCACGGACC CTGCGATTCA ATCCAGCCAC 
     1861 ATAAAAATAT CAAAATCACG TTCGAGGAAG ATAAGGTGAA TTCTTCTCTC GTCGTGGACC 
     1921 GCGAATCTAG TCACGATGGC TGTCAGGACG CGCTGAACAT TCTTCCTGTA CCGGGACCGA 
     1981 CATCTAGCGC AACCAACGTG TCAATGGTTG TGTCTGCAGG ACCGTTGAGC TCAGAAAAAG 
     2041 CAGAGATGAA TATCCTGGAG ATAAATGAGA AGTTGTGTCC TCAACTGGCA GAGAAGAAGC 
     2101 AGCAGTTCCG CTCCCTGAAG GAAAAGTGTT TTGTGACGCA GGTCGCTTGC TTCCTGGCCA 
     2161 AACAGCAGAA TAAATACAAA TATGAGGAAT GCAAAGATCT CATCAAAAGT ATGTTGAGGA 
     2221 ACGAGCGACA GTTTAAGGAA GAAAAATTGG CAGAACAGTT GAAGCAGGCA GAGGAACTGC 
     2281 GACAATATAA GGTCCTTGTG CACTCTCAGG AAAGGGAACT GACTCAGCTC AGAGAGAAGT 
     2341 TGAGGGAGGG CCGCGACGCT TCTAGGTCCC TCAATGAACA CTTGCAAGCG CTTCTGACAC 
     2401 CCGACGAGCC AGATAAATCA CAAGGCCAGG ACCTGCAGGA ACAACTCGCG GAGGGCTGCC 
     2461 GCCTGGCCCA GCACCTGGTA CAGAAGCTTT CCCCTGAGAA CGACAATGAC GACGACGAGG 
     2521 ACGTTCAAGT AGAAGTAGCT GAGAAGGTTC AGAAATCAAG CAGCCCAAGA GAAATGCAGA 
     2581 AAGCAGAAGA AAAGGAAGTG CCCGAGGACA GCCTCGAAGA ATGCGCCATA ACATGTTCCA 
     2641 ACAGCCATGG CCCTTATGAT AGCAATCAGC CCCACAGAAA GACCAAGATC ACATTCGAAG 
     2701 AGGATAAAGT CGACTCCACC TTGATCGGGT CCAGCTCCCA TGTCGAATGG GAGGACGCTG 
     2761 TCCATATCAT TCCAGAGAAC GAATCAGACG ACGAGGAGGA GGAAGAAAAA GGACCTGTGA 
     2821 GCCCTCGAAA CCTGCAAGAA TCTGAAGAAG AAGAGGTGCC CCAGGAGAGC TGGGATGAGG 
     2881 GCTACTCAAC TCTGAGCATT CCCCCTGAGA GGCTGGCGAG TTACCAGAGT TATAGTAGCA 
     2941 CTTTCCATTC ACTGGAAGAG CAGCAGGTCT GTATGGCTGT GGACATAGGT AGGCATAGGT 
     3001 GGGATCAAGT GAAGAAGGAA GACCAAGAGG CCACCGGCCC ACGCCTGTCT CGCGAATTGT 
     3061 TGGACGAAAA GGAACCTGAA GTGCTGCAGG ATAGCCTGGA TCGGTGCTAT AGCACGCCAA 
     3121 GTGTGTACCT GGGCTTGACA GATTCCTGTC AACCATACAG GTCAGCATTC TACGTTCTTG 
     3181 AGCAGCAGAG AATCGGCCTG GCGGTCGACA TGGATGAGAT TGAGAAGTAC CAGGAGGTAG 
     3241 AAGAAGACCA GGATCCGAGC TGTCCAAGGC TGTCCCGAGA GCTCCTGGCT GAGAAGGAGC 
     3301 CCGAAGTTCT CCAGGACTCT CTCGATCGGT GTTATTCAAC ACCAAGCGGT TACCTTGAAT 
     3361 TGCCCGACCT GGGACAGCCA TACCGGAGCG CGGTGTATTC TCTCGAGGAA CAGTACTTGG 
     3421 GTCTGGCTCT GGACGTCGAT CGAATCAAGA AAGATCAGGA GGAGGAAGAA GATCAGGGAC 
     3481 CTCCATGCCC CAGACTTTCA AGGGAACTGC TCGAGGTTGT CGAACCCGAG GTTCTCCAAG 
     3541 ATAGCCTCGA CGTGATTCAA CTGCTGCCAG TTGTGCTCAA CAGCTTGACA CCTGCTAGCC 
     3601 CTACTGAGGT GCCTTTTATG CATTGGAGAA AGAATATGTT GGCATTTCTC CTTACATGGG 
     3661 AGAAGCTTAA ACGGCGCGGC CGCGGACGCA AGGAAGGAGA GGAAGATCAG AGAAGGAAAG 
     3721 AGGAGGGGGA GGAGAAAAAG GGCAAGAAAA TAAAGACACA TCACGCTCCT GGAAGTGCCG 
     3781 CCTGTACGCG TACGCGGCCG CTCGAGATGG AGAGCGACGA GAGCGGCCTG CCCGCCATGG 
     3841 AGATCGAGTG CCGCATCACC GGCACCCTGA ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG 
     3901 GAGAGGGCAC CCCCGAGCAG GGCCGCATGA CCAACAAGAT GAAGAGCACC AAAGGCGCCC 
     3961 TGACCTTCAG CCCCTACCTG CTGAGCCACG TGATGGGCTA CGGCTTCTAC CACTTCGGCA 
     4021 CCTACCCCAG CGGCTACGAG AACCCCTTCC TGCACGCCAT CAACAACGGC GGCTACACCA 
     4081 ACACCCGCAT CGAGAAGTAC GAGGACGGCG GCGTGCTGCA CGTGAGCTTC AGCTACCGCT 
     4141 ACGAGGCCGG CCGCGTGATC GGCGACTTCA AGGTGATGGG CACCGGCTTC CCCGAGGACA 
     4201 GCGTGATCTT CACCGACAAG ATCATCCGCA GCAACGCCAC CGTGGAGCAC CTGCACCCCA 
     4261 TGGGCGATAA CGATCTGGAT GGCAGCTTCA CCCGCACCTT CAGCCTGCGC GACGGCGGCT 
     4321 ACTACAGCTC CGTGGTGGAC AGCCACATGC ACTTCAAGAG CGCCATCCAC CCCAGCATCC 
     4381 TGCAGAACGG GGGCCCCATG TTCGCCTTCC GCCGCGTGGA GGAGGATCAC AGCAACACCG 
     4441 AGCTGGGCAT CGTGGAGTAC CAGCACGCCT TCAAGACCCC GGATGCAGAT GCCGGTGAAG 
     4501 AAAGAGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4561 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4621 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4681 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4741 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4801 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4861 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4921 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4981 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5041 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5101 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5161 ATGCCTGTTG AATTGGGTAC GCGGCCAGCT TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT 
     5221 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5281 GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5341 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG 
     5401 CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC 
     5461 GAGGCCGCCT CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA 
     5521 GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA 
     5581 GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT 
     5641 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 
     5701 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC 
     5761 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 
     5821 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG 
     5881 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC 
     5941 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 
     6001 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT 
     6061 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 
     6121 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG 
     6181 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG 
     6241 GCCGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 
     6301 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC 
     6361 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 
     6421 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA 
     6481 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     6541 TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT 
     6601 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG 
     6661 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA 
     6721 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC 
     6781 CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT 
     6841 AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 
     6901 AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT 
     6961 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG 
     7021 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 
     7081 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT 
     7141 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 
     7201 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT 
     7261 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 
     7321 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 
     7381 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT 
     7441 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 
     7501 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA 
     7561 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 
     7621 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTTTTT 
     7681 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
     7741 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
     7801 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
     7861 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
     7921 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
     7981 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
     8041 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
     8101 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
     8161 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
     8221 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
     8281 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
     8341 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
     8401 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
     8461 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
     8521 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
     8581 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
     8641 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
     8701 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
     8761 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
     8821 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
     8881 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 
     8941 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 
     9001 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 
     9061 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 
     9121 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 
     9181 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 
     9241 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 
     9301 AATTTAACGC GAATTTT
//