LOCUS       RG218656                9338 bp    DNA     circular  SYN 
DEFINITION  GPRC6A (tGFP-tagged) - Human G protein-coupled receptor, family C, group 6, member A (GPRC6A)
ACCESSION   NM_148963
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3819..4523 
                     /label="GFP"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    7247..7892 
                     /label="ColE1"  
     misc_feature    5609..6403 
                     /label="Neo"  
     misc_feature    5240..5542 
                     /label="SV40 ori"  
     misc_feature    7907..8767 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3798..3804 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3798 
                     /label="GPRC6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATTCTTA ATTATACTAA TTACCTGCTT TGTGATTATT CTTGCTACTT 
     1081 CACAGCCTTG CCAGACCCCT GATGACTTTG TGGCTGCCAC TTCTCCGGGA CATATCATAA 
     1141 TTGGAGGTTT GTTTGCTATT CATGAAAAAA TGTTGTCCTC AGAAGACTCT CCCAGACGAC 
     1201 CACAAATCCA GGAGTGTGTT GGCTTTGAAA TATCAGTTTT TCTTCAAACT CTTGCCATGA 
     1261 TACACAGCAT TGAGATGATC AACAATTCAA CACTCTTATC TGGAGTCAAA CTGGGGTATG 
     1321 AAATCTATGA CACTTGTACA GAAGTCACAG TGGCAATGGC AGCCACTCTG AGGTTTCTTT 
     1381 CTAAATTCAA CTGCTCCAGA GAAACTGTGG AGTTTAAGTG TGACTATTCC AGCTACATGC 
     1441 CAAGAGTTAA GGCTGTCATA GGTTCTGGGT ACTCAGAAAT AACTATGGCT GTCTCCAGGA 
     1501 TGTTGAATTT ACAGCTCATG CCACAGGTGG GTTATGAATC AACTGCAGAA ATCCTGAGTG 
     1561 ACAAAATTCG CTTTCCTTCA TTTTTACGGA CTGTGCCCAG TGACTTCCAT CAAATTAAAG 
     1621 CAATGGCTCA CCTGATTCAG AAATCTGGTT GGAACTGGAT TGGCATCATA ACCACAGATG 
     1681 ATGACTATGG ACGATTGGCT CTTAACACTT TTATAATTCA GGCTGAAGCA AATAACGTGT 
     1741 GCATAGCCTT CAAAGAGGTT CTTCCAGCCT TTCTTTCAGA TAATACCATT GAAGTCAGAA 
     1801 TCAATCGGAC ACTGAAGAAA ATCATTTTAG AAGCCCAGGT TAATGTCATT GTGGTATTTC 
     1861 TGAGGCAATT CCATGTTTTT GATCTCTTCA ATAAAGCCAT TGAAATGAAT ATAAATAAGA 
     1921 TGTGGATTGC TAGTGATAAT TGGTCAACTG CCACCAAGAT TACCACCATT CCTAATGTTA 
     1981 AAAAGATTGG CAAAGTTGTA GGGTTTGCCT TTAGAAGAGG GAATATATCC TCTTTCCATT 
     2041 CCTTTCTTCA AAATCTGCAC TTGCTTCCCA GTGACAGTCA CAAACTCTTA CATGAATATG 
     2101 CCATGCATTT ATCTGCCTGC GCATATGTCA AGGACACTGA TTTGAGTCAA TGCATATTCA 
     2161 ATCATTCTCA AAGGACTTTG GCCTACAAGG CTAACAAGGC TATAGAAAGG AACTTCGTCA 
     2221 TGAGAAATGA CTTCCTCTGG GACTATGCTG AGCCAGGACT CATTCATAGT ATTCAGCTTG 
     2281 CAGTGTTTGC CCTTGGTTAT GCCATTCGGG ATCTGTGTCA AGCTCGTGAC TGTCAGAACC 
     2341 CCAACGCCTT TCAACCATGG GAGTTACTTG GTGTGCTAAA AAATGTGACA TTCACTGATG 
     2401 GATGGAATTC ATTTCATTTT GATGCTCACG GGGATTTAAA TACTGGATAT GATGTTGTGC 
     2461 TCTGGAAGGA GATCAATGGA CACATGACTG TCACTAAGAT GGCAGAATAT GACCTACAGA 
     2521 ATGATGTCTT CATCATCCCA GATCAGGAAA CAAAAAATGA GTTCAGGAAT CTTAAGCAAA 
     2581 TTCAATCTAA ATGCTCCAAG GAATGCAGTC CTGGGCAAAT GAAGAAAACT ACAAGAAGTC 
     2641 AACACATCTG TTGCTATGAA TGTCAGAACT GTCCTGAAAA TCATTACACT AATCAGACAG 
     2701 ATATGCCTCA CTGCCTTTTA TGCAACAACA AAACTCACTG GGCCCCTGTT AGGAGCACTA 
     2761 TGTGCTTTGA AAAGGAAGTG GAATATCTCA ACTGGAATGA CTCCTTGGCC ATCCTACTCC 
     2821 TGATTCTCTC CCTACTGGGA ATCATATTTG TTCTGGTTGT TGGCATAATA TTTACAAGAA 
     2881 ACCTGAACAC ACCTGTTGTG AAATCATCCG GGGGATTAAG AGTCTGCTAT GTGATCCTTC 
     2941 TCTGTCATTT CCTCAATTTT GCCAGCACGA GCTTTTTCAT TGGAGAACCA CAAGACTTCA 
     3001 CATGTAAAAC CAGGCAGACA ATGTTTGGAG TGAGCTTTAC TCTTTGCATC TCCTGCATTT 
     3061 TGACGAAGTC TCTGAAAATT TTGCTAGCCT TCAGCTTTGA TCCCAAATTA CAGAAATTTC 
     3121 TGAAGTGCCT CTATAGACCG ATCCTTATTA TCTTCACTTG CACGGGCATC CAGGTTGTCA 
     3181 TTTGCACACT CTGGCTAATC TTTGCAGCAC CTACTGTAGA GGTGAATGTC TCCTTGCCCA 
     3241 GAGTCATCAT CCTGGAGTGT GAGGAGGGAT CCATACTTGC ATTTGGCACC ATGCTGGGCT 
     3301 ACATTGCCAT CCTGGCCTTC ATTTGCTTCA TATTTGCTTT CAAAGGCAAA TATGAGAATT 
     3361 ACAATGAAGC CAAATTCATT ACATTTGGCA TGCTCATTTA CTTCATAGCT TGGATCACAT 
     3421 TCATCCCTAT CTATGCTACC ACATTTGGCA AATATGTACC AGCTGTGGAG ATTATTGTCA 
     3481 TATTAATATC TAACTATGGA ATCCTGTATT GCACATTCAT CCCCAAATGC TATGTTATTA 
     3541 TTTGTAAGCA AGAGATTAAC ACAAAGTCTG CCTTTCTCAA GATGATCTAC AGTTATTCTT 
     3601 CCCATAGTGT GAGCAGCATT GCCCTGAGTC CTGCTTCACT GGACTCCATG AGCGGCAATG 
     3661 TCACAATGAC CAATCCCAGC TCTAGTGGCA AGTCTGCAAC CTGGCAGAAA AGCAAAGATC 
     3721 TTCAGGCACA AGCATTTGCA CACATATGCA GGGAAAATGC CACAAGTGTA TCTAAAACTT 
     3781 TGCCTCGAAA AAGAATGTCA AGTATAACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3841 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3901 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3961 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     4021 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4081 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4141 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4201 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4261 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4321 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4381 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4441 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4501 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5221 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5281 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5341 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5401 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5461 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5521 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5581 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5641 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5701 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5761 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5821 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5881 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5941 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     6001 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6061 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6121 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6181 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6241 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6301 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6361 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6421 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6481 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6541 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6601 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6661 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6721 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6781 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6841 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6901 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6961 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     7021 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7081 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7141 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7201 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7261 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7321 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7381 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7441 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7501 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7561 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7621 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7681 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7741 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7801 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7861 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7921 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7981 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     8041 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8101 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8161 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8221 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8281 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8341 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8401 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8461 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8521 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8581 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8641 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8701 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8761 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8821 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8881 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8941 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     9001 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9061 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9121 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9181 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9241 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9301 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//