LOCUS       RG218103                9176 bp    DNA     circular  SYN 
DEFINITION  GRM2 (GFP-tagged) - Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1
ACCESSION   NM_000839
VERSION     NM_000839.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3657..4361 
                     /label="GFP"  
     misc_feature    4408..4993 
                     /label="PolyA signal"  
     misc_feature    7085..7730 
                     /label="ColE1"  
     misc_feature    5447..6241 
                     /label="Neo"  
     misc_feature    5078..5380 
                     /label="SV40 ori"  
     misc_feature    7745..8605 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3636..3642 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3636 
                     /label="GRM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGATCGCTG CTTGCGCTCC TGGCACTGCT GCTGCTGTGG GGTGCTGTGG 
     1081 CTGAGGGCCC AGCCAAGAAG GTGCTGACCC TGGAGGGAGA CTTGGTGCTG GGTGGGCTGT 
     1141 TCCCAGTGCA CCAGAAGGGC GGCCCAGCAG AGGACTGTGG TCCTGTCAAT GAGCACCGTG 
     1201 GCATCCAGCG CCTGGAGGCC ATGCTTTTTG CACTGGACCG CATCAACCGT GACCCGCACC 
     1261 TGCTGCCTGG CGTGCGCCTG GGTGCACACA TCCTCGACAG TTGCTCCAAG GACACACATG 
     1321 CGCTGGAGCA GGCACTGGAC TTTGTGCGTG CCTCACTCAG CCGTGGTGCT GATGGCTCAC 
     1381 GCCACATCTG CCCCGACGGC TCTTATGCGA CCCATGGTGA TGCTCCCACT GCCATCACTG 
     1441 GTGTTATTGG CGGTTCCTAC AGTGATGTCT CCATCCAGGT GGCCAACCTC TTGAGGCTAT 
     1501 TTCAGATCCC ACAGATTAGC TACGCCTCTA CCAGTGCCAA GCTGAGTGAC AAGTCCCGCT 
     1561 ATGACTACTT TGCCCGCACA GTGCCTCCTG ACTTCTTCCA AGCCAAGGCC ATGGCTGAGA 
     1621 TTCTCCGCTT CTTCAACTGG ACCTATGTGT CCACTGTGGC GTCTGAGGGC GACTATGGCG 
     1681 AGACAGGCAT TGAGGCCTTT GAGCTAGAGG CTCGTGCCCG CAACATCTGT GTGGCCACCT 
     1741 CGGAGAAAGT GGGCCGTGCC ATGAGCCGCG CGGCCTTTGA GGGTGTGGTG CGAGCCCTGC 
     1801 TGCAGAAGCC CAGTGCCCGC GTGGCTGTCC TGTTCACCCG TTCTGAGGAT GCCCGGGAGC 
     1861 TGCTTGCTGC CAGCCAGCGC CTCAATGCCA GCTTCACCTG GGTGGCCAGT GATGGTTGGG 
     1921 GGGCCCTGGA GAGTGTGGTG GCAGGCAGTG AGGGGGCTGC TGAGGGTGCT ATCACCATCG 
     1981 AGCTGGCCTC CTACCCCATC AGTGACTTTG CCTCCTACTT CCAGAGCCTG GACCCTTGGA 
     2041 ACAACAGCCG GAACCCCTGG TTCCGTGAAT TCTGGGAGCA GAGGTTCCGC TGCAGCTTCC 
     2101 GGCAGCGAGA CTGCGCAGCC CACTCTCTCC GGGCTGTGCC CTTTGAGCAG GAGTCCAAGA 
     2161 TCATGTTTGT GGTCAATGCA GTGTACGCCA TGGCCCATGC GCTCCACAAC ATGCACCGTG 
     2221 CCCTCTGCCC CAACACCACC CGGCTCTGTG ACGCGATGCG GCCAGTTAAC GGGCGCCGCC 
     2281 TCTACAAGGA CTTTGTGCTC AACGTCAAGT TTGATGCCCC CTTTCGCCCA GCTGACACCC 
     2341 ACAATGAGGT CCGCTTTGAC CGCTTTGGTG ATGGTATTGG CCGCTACAAC ATCTTCACCT 
     2401 ATCTGCGTGC AGGCAGTGGG CGCTATCGCT ACCAGAAGGT GGGCTACTGG GCAGAAGGCT 
     2461 TGACTCTGGA CACCAGCCTC ATCCCATGGG CCTCACCCTC AGCCGGCCCC CTGCCCGCCT 
     2521 CTCGCTGCAG TGAGCCCTGC CTCCAGAATG AGGTGAAGAG TGTGCAGCCG GGCGAAGTCT 
     2581 GCTGCTGGCT CTGCATTCCG TGCCAGCCCT ATGAGTACCG ATTGGACGAA TTCACTTGCG 
     2641 CTGATTGTGG CCTGGGCTAC TGGCCCAATG CCAGCCTGAC TGGCTGCTTC GAACTGCCCC 
     2701 AGGAGTACAT CCGCTGGGGC GATGCCTGGG CTGTGGGACC TGTCACCATC GCCTGCCTCG 
     2761 GTGCCCTGGC CACCCTCTTT GTGCTGGGTG TCTTTGTGCG GCACAATGCC ACACCAGTGG 
     2821 TCAAGGCCTC AGGTCGGGAG CTCTGCTACA TCCTGCTGGG TGGTGTCTTC CTCTGCTACT 
     2881 GCATGACCTT CATCTTCATT GCCAAGCCAT CCACGGCAGT GTGTACCTTA CGGCGTCTTG 
     2941 GTTTGGGCAC TGCCTTCTCT GTCTGCTACT CAGCCCTGCT CACCAAGACC AACCGCATTG 
     3001 CACGCATCTT CGGTGGGGCC CGGGAGGGTG CCCAGCGGCC ACGCTTCATC AGTCCTGCCT 
     3061 CACAGGTGGC CATCTGCCTG GCACTTATCT CGGGCCAGCT GCTCATCGTG GTCGCCTGGC 
     3121 TGGTGGTGGA GGCACCGGGC ACAGGCAAGG AGACAGCCCC CGAACGGCGG GAGGTGGTGA 
     3181 CACTGCGCTG CAACCACCGC GATGCAAGTA TGTTGGGCTC GCTGGCCTAC AATGTGCTCC 
     3241 TCATCGCGCT CTGCACGCTT TATGCCTTCA AGACTCGCAA GTGCCCCGAA AACTTCAACG 
     3301 AGGCCAAGTT CATTGGCTTC ACCATGTACA CCACCTGCAT CATCTGGCTG GCATTCCTGC 
     3361 CCATCTTCTA TGTCACCTCC AGTGACTACC GGGTACAGAC CACCACCATG TGCGTGTCAG 
     3421 TCAGCCTCAG CGGCTCCGTG GTGCTTGGCT GCCTCTTTGC GCCCAAGCTG CACATCATCC 
     3481 TCTTCCAGCC GCAGAAGAAC GTGGTTAGCC ACCGGGCACC CACCAGCCGC TTTGGCAGTG 
     3541 CTGCTGCCAG GGCCAGCTCC AGCCTTGGCC AAGGGTCTGG CTCCCAGTTT GTCCCCACTG 
     3601 TTTGCAATGG CCGTGAGGTG GTGGACTCGA CAACGTCATC GCTTACGCGT ACGCGGCCGC 
     3661 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3721 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3781 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3841 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3901 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3961 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     4021 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4081 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4141 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4201 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4261 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4321 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4381 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4441 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4501 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4561 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4621 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4681 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4741 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4801 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4861 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4921 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4981 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5041 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5101 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5161 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5221 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5281 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5341 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5401 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5461 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5521 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5581 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5641 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5701 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5761 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5821 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5881 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5941 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     6001 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6061 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6121 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6181 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6241 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6301 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6361 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6421 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6481 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6541 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6601 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6661 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6721 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6781 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6841 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6901 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6961 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     7021 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7081 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7141 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7201 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7261 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7321 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7381 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7441 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7501 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7561 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7621 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7681 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7741 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7801 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7861 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7921 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7981 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     8041 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8101 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8161 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8221 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8281 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8341 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8401 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8461 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8521 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8581 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8641 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8701 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8761 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8821 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8881 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8941 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     9001 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9061 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9121 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//