LOCUS       RG217952                8849 bp    DNA     circular  SYN 
DEFINITION  ANKFN1 (GFP-tagged) - Human ankyrin-repeat and fibronectin type III domain containing 1 (ANKFN1)
ACCESSION   NM_153228
VERSION     NM_153228.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3330..4034 
                     /label="GFP"  
     misc_feature    4081..4666 
                     /label="PolyA signal"  
     misc_feature    6758..7403 
                     /label="ColE1"  
     misc_feature    5120..5914 
                     /label="Neo"  
     misc_feature    4751..5053 
                     /label="SV40 ori"  
     misc_feature    7418..8278 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3309..3315 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3309 
                     /label="ANKFN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGTCT CTAACCAGGA GGCTACTCTT TAAAGACAGG CATTTTACTT 
     1081 GCAGCAAAAT AATAGGAAGG AGATTCGCTT GCTTTGCACA GAGGCTGAGC CACAGGAGAA 
     1141 AGCAAAGCCA ATGTGATTTA TTGAATGAAA GCACTGGACA ATTACCAACA ACTTGTTCCT 
     1201 CTGCTGCCTC GAACAGCATA AACTGGAATT GTCGTGTGAA AATGACGCAA CAAATGCAAA 
     1261 ATTTACATCT CTGTCAGTCA AAAAAACATA GTGCTCCCTC ATCTCCCAAC GCAGCCAAAC 
     1321 GCCTGTACAG GAACCTCTCT GAGAAACTGA AAGGGAGCCA CTCTTCCTTC GATGAGGCCT 
     1381 ATTTTAGGAC AAGAACTGAT CGGCTGAGTC TCAGGAAGAC CTCGGTGAAT TTCCAGGGCA 
     1441 ATGAAGCCAT GTTTGAGGCA GTCGAACAGC AGGACATGGA TGCTGTGCAG ATCCTCCTGT 
     1501 ATCAGTACAC ACCAGAAGAA CTTGACCTCA ACACACCTAA CAGCGAGGGC TTGACACCCC 
     1561 TGGATATTGC CATCATGACC AACAATGTGC CCATTGCAAG GATTCTTCTG AGGACAGGGG 
     1621 CCCGAGAAAG TCCACACTTT GTCAGCCTGG AAAGCCGAGC AATGCACCTA AACACACTGG 
     1681 TCCAGGAAGC CCAGGAGAGG GTGAGTGAAC TGTCTGCCCA GGTGGAGAAT GAAGGATTCA 
     1741 CTCTGGACAA CACAGAGAAA GAGAAACAGC TGAAAGCTTG GGAGTGGAGG TATCGGCTCT 
     1801 ACAGACGCAT GAAAACAGGC TTTGAGCATG CCAGAGCCCC TGAGATGCCA ACCAATGTCT 
     1861 GTCTCATGGT AACCAGCAGC ACATCACTCA CTGTCAGCTT CCAAGAGCCT CTTAGCGTCA 
     1921 ATGCAGCTGT AGTAACCAGG TATAAAGTGG AATGGAGTAT GTCCGAAGAC TTTTCTCCTT 
     1981 TGGCTGGAGA AATCATCATG GATAATCTGC AGACTCTGAG ATGCACAATC ACAGGACTTA 
     2041 CAATGGGCCA ACAGTATTTT GTTCAAGTCT CGGCTTACAA TATGAAAGGA TGGGGACCTG 
     2101 CTCAGACCAC GACACCGGCA TGTGCCTCTC CTTCTAACTG GAAAGACTAT GACGACAGAG 
     2161 AGCCCAGACA CAAGGGACAG AGTGAAGTTT TGGAAGGTCT GCTGCAGCAG GTCCGAGCCC 
     2221 TTCATCAGCA TTACAGTTGC CGGGAAAGCA CAAAATTACA AACCACAGGC CGCAAGCAGT 
     2281 CAGTCTCAAG AAGCCTGAAA CACCTGTTCC ATTCCTCGAA CAAGTTTGTG AAGACCTTAA 
     2341 AACGGGGACT CTACATAGCC ATTATATTTT ATTACAAAGA CAATATCTTA GTCACCAATG 
     2401 AAGATCAAGT ACCAATTGTT GAAATAGATG ACTCTCACAC CAGTTCTATT ACACAAGATT 
     2461 TTCTGTGGTT CACGAAGCTG TCTTGTATGT GGGAAGATAT AAGGTGGCTG AGGCAAAGCA 
     2521 TACCAATATC CTCATCCTCA TCCACAGTGC TGCAAACTCG GCAGAAGATG CTCGCAGCAA 
     2581 CAGCACAGCT ACAGAATTTA CTTGGGACAC ACAACTTGGG AAGAGTTTAC TATGAGCCCA 
     2641 TTAAAGATCG ACATGGAAAC ATACTCATAG TCACCATCAG GGAGGTGGAG ATGCTTTATT 
     2701 CATTTTTTAA TGGCAAATGG ATGCAGATCT CAAAGCTGCA AAGCCAGAGA AAGTCTCTAT 
     2761 CAACACCTGA GGAGCCAACA GCTTTAGACA TTCTACTGAT AACCATCCAG GATATTCTAT 
     2821 CCTATCACAA AAGGAGTCAT CAGCGTCTCT TTCCTGGATT ATATCTGGGT TACCTAAAGC 
     2881 TCTGTAGCTC TGTGGATCAA ATCAAAGTTC TTGTTACCCA AAAGTTGCCC AACATTCTCT 
     2941 GCCACGTGAA GATCCGTGAA AACAATAATA TTTCTAGAGA GGAATGGGAA TGGATCCAAA 
     3001 AGCTTTCTGG CTCTGAATCT ATGGAAAGTG TGGATCATAC TTCTGACTGC CCCATGCAAT 
     3061 TGTTCTTCTA CGAGCTCCAG ATGGCAGTGA AAGCTCTCCT TCAGCAGATC AATATACCTC 
     3121 TACACCAGGC AAGGAACTTC CGCCTCTACA CACAGGAGGT GTTGGAAATG GGTCACAATG 
     3181 TGTCCTTTCT TCTCCTGCTC CCTGCCTCAG ACGACGTCTG TACAGCCCCA GGACAGAATA 
     3241 ATCCTTACAC CCCACACTCA GGGTTTCTTA ACCTCCCTCT TCAGATGTTT GAACTTGGTA 
     3301 TAGTAGCTTG TTTCACCACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3361 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3421 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3481 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3541 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3601 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     3661 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     3721 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     3781 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     3841 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     3901 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     3961 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4021 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4081 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4141 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4201 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4261 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4321 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4381 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4441 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4501 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4561 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4621 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4681 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     4741 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     4801 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4861 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     4921 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     4981 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5041 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5101 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5161 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5221 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5281 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5341 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5401 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5461 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5521 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5581 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     5641 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     5701 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     5761 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     5821 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     5881 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     5941 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6001 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6061 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6121 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6181 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6241 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6301 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6361 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6421 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6481 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6541 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6601 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     6661 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     6721 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     6781 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     6841 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     6901 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     6961 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7021 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7081 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7141 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7201 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7261 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7321 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7381 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7441 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7501 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7561 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7621 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     7681 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     7741 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     7801 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     7861 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     7921 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     7981 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8041 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8101 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8161 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8221 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8281 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8341 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8401 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8461 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8521 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8581 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     8641 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     8701 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     8761 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     8821 TGATTTAACA AAAATTTAAC GCGAATTTT
//