LOCUS       RG217448                9242 bp    DNA     circular  SYN 
DEFINITION  MAP4K3 (GFP-tagged) - Human mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3)
ACCESSION   NM_003618
VERSION     NM_003618.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3723..4427 
                     /label="GFP"  
     misc_feature    4474..5059 
                     /label="PolyA signal"  
     misc_feature    7151..7796 
                     /label="ColE1"  
     misc_feature    5513..6307 
                     /label="Neo"  
     misc_feature    5144..5446 
                     /label="SV40 ori"  
     misc_feature    7811..8671 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3702..3708 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3702 
                     /label="MAP4K3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCCCGGC TTCGATTTGT CCCGCCGGAA CCCGCAGGAG GACTTCGAGC 
     1081 TGATTCAGCG CATCGGCAGC GGCACCTACG GCGACGTCTA CAAGGCACGG AATGTTAACA 
     1141 CTGGTGAATT AGCAGCAATT AAAGTAATAA AATTGGAACC AGGAGAAGAC TTTGCAGTTG 
     1201 TGCAGCAAGA AATTATCATG ATGAAAGACT GTAAACACCC AAATATTGTT GCTTATTTTG 
     1261 GAAGCTATCT CAGGCGAGAT AAGCTTTGGA TTTGCATGGA GTTTTGTGGA GGTGGTTCTT 
     1321 TACAGGATAT TTATCACGTA ACTGGACCTC TGTCAGAACT GCAAATTGCA TATGTTAGCA 
     1381 GAGAAACACT GCAGGGATTA TATTATCTTC ACAGTAAAGG AAAAATGCAC AGAGATATAA 
     1441 AGGGAGCTAA CATTCTATTA ACGGATAATG GTCATGTGAA ATTGGCTGAT TTTGGAGTAT 
     1501 CTGCACAGAT AACAGCTACA ATTGCCAAAC GGAAGTCTTT CATTGGCACA CCATATTGGA 
     1561 TGGCTCCAGA AGTTGCAGCT GTTGAGAGGA AGGGGGGTTA CAATCAACTC TGTGATCTCT 
     1621 GGGCAGTGGG AATCACTGCC ATAGAACTTG CAGAGCTTCA GCCTCCTATG TTTGACTTAC 
     1681 ACCCAATGAG AGCATTATTT CTAATGACAA AAAGCAATTT TCAGCCTCCT AAACTAAAGG 
     1741 ATAAAATGAA ATGGTCAAAT AGTTTTCATC ACTTTGTGAA AATGGCACTT ACCAAAAATC 
     1801 CGAAAAAAAG ACCTACTGCT GAAAAATTAT TACAGCATCC TTTTGTAACA CAACATTTGA 
     1861 CACGGTCTTT GGCAATCGAG CTGTTGGATA AAGTAAATAA TCCAGATCAT TCCACTTACC 
     1921 ATGATTTCGA TGATGATGAT CCTGAGCCTC TTGTTGCTGT ACCACATAGA ATTCACTCAA 
     1981 CAAGTAGAAA CGTGAGAGAA GAAAAAACAC GCTCAGAGAT AACCTTTGGC CAAGTGAAAT 
     2041 TTGATCCACC CTTAAGAAAG GAGACAGAAC CACATCATGA ACTTCCCGAC AGTGATGGTT 
     2101 TTTTGGACAG TTCAGAAGAA ATATACTACA CTGCAAGATC TAATCTGGAT CTGCAACTGG 
     2161 AATATGGACA AGGACACCAA GGTGGTTACT TTTTAGGTGC AAACAAGAGT CTTCTCAAGT 
     2221 CTGTTGAAGA AGAATTGCAT CAGCGAGGAC ACGTCGCACA TTTAGAAGAT GATGAAGGAG 
     2281 ATGATGATGA ATCTAAACAC TCAACTCTGA AAGCAAAAAT TCCACCTCCT TTGCCACCAA 
     2341 AGCCTAAGTC TATCTTCATA CCACAGGAAA TGCATTCTAC TGAGGATGAA AATCAAGGAA 
     2401 CAATCAAGAG ATGTCCCATG TCAGGGAGCC CAGCAAAGCC ATCCCAAGTT CCACCTAGAC 
     2461 CACCACCTCC CAGATTACCC CCACACAAAC CTGTTGCCTT AGGAAATGGA ATGAGCTCCT 
     2521 TCCAGTTAAA TGGTGAACGA GATGGCTCAT TATGTCAACA ACAGAATGAA CATAGAGGCA 
     2581 CAAACCTTTC AAGAAAAGAA AAGAAAGATG TACCAAAGCC TATTAGTAAT GGTCTTCCTC 
     2641 CAACACCTAA AGTGCATATG GGTGCATGTT TTTCAAAAGT TTTTAATGGG TGTCCCTTGA 
     2701 AAATTCACTG TGCATCATCA TGGATAAACC CAGATACAAG AGATCAGTAC TTGATATTTG 
     2761 GTGCCGAAGA AGGGATTTAT ACCCTCAATC TTAATGAACT TCATGAAACA TCAATGGAAC 
     2821 AGCTATTCCC TCGAAGGTGT ACATGGTTGT ATGTAATGAA CAATTGCTTG CTATCAATAT 
     2881 CTGGTAAAGC TTCTCAGCTT TATTCCCATA ATTTACCAGG GCTTTTTGAT TATGCAAGAC 
     2941 AAATGCAAAA GTTACCTGTT GCTATTCCAG CACACAAACT CCCTGACAGA ATACTGCCAA 
     3001 GGAAATTTTC TGTATCAGCA AAAATCCCTG AAACCAAATG GTGCCAGAAG TGTTGTGTTG 
     3061 TAAGAAATCC TTACACGGGC CATAAATACC TATGTGGAGC ACTTCAGACT AGCATTGTTC 
     3121 TATTAGAATG GGTTGAACCA ATGCAGAAAT TTATGTTAAT TAAGCACATA GATTTTCCTA 
     3181 TACCATGTCC ACTTAGAATG TTTGAAATGC TGGTAGTTCC TGAACAGGAG TACCCTTTAG 
     3241 TTTGTGTTGG TGTCAGTAGA GGTAGAGACT TCAACCAAGT GGTTCGATTT GAGACGGTCA 
     3301 ATCCAAATTC TACCTCTTCA TGGTTTACAG AATCAGATAC CCCACAGACA AATGTTACTC 
     3361 ATGTAACCCA ACTGGAGAGA GATACCATCC TTGTATGCTT GGACTGTTGT ATAAAAATAG 
     3421 TAAATCTCCA AGGAAGATTA AAATCTAGCA GGAAATTGTC ATCAGAACTC ACCTTTGATT 
     3481 TCCAGATTGA ATCAATAGTG TGCCTACAAG ACAGTGTGCT AGCTTTCTGG AAACATGGAA 
     3541 TGCAAGGTAG AAGTTTTAGA TCTAATGAGG TAACACAAGA AATTTCAGAT AGCACAAGAA 
     3601 TTTTCAGGCT GCTTGGATCT GACAGGGTCG TGGTTTTGGA AAGTAGGCCA ACTGATAACC 
     3661 CCACAGCAAA TAGCAATTTG TACATCCTGG CGGGTCATGA AAACAGTTAC ACGCGTACGC 
     3721 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3781 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3841 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3901 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3961 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     4021 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     4081 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     4141 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     4201 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     4261 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     4321 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     4381 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4441 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4501 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4561 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4621 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4681 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4741 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4801 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4861 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4921 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4981 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5041 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5101 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     5161 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     5221 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5281 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     5341 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     5401 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5461 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5521 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5581 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5641 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5701 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5761 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5821 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5881 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5941 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     6001 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     6061 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     6121 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     6181 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     6241 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     6301 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     6361 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6421 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6481 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6541 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6601 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6661 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6721 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6781 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6841 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6901 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6961 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     7021 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     7081 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     7141 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     7201 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     7261 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     7321 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     7381 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7441 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7501 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7561 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7621 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7681 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7741 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7801 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7861 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7921 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7981 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     8041 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     8101 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     8161 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     8221 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     8281 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     8341 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     8401 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8461 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8521 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8581 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8641 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8701 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8761 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8821 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8881 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8941 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     9001 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     9061 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     9121 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     9181 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     9241 TT
//