LOCUS       RG217200                9446 bp    DNA     circular  SYN 
DEFINITION  PCSK6 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 8
ACCESSION   NM_138321
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3927..4631 
                     /label="GFP"  
     misc_feature    4678..5263 
                     /label="PolyA signal"  
     misc_feature    7355..8000 
                     /label="ColE1"  
     misc_feature    5717..6511 
                     /label="Neo"  
     misc_feature    5348..5650 
                     /label="SV40 ori"  
     misc_feature    8015..8875 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3906..3912 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3906 
                     /label="PCSK6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCGCGC GCGCCGCCTG CGCCCGGGCC CCGGCCGCCG CCCCGGGCCG 
     1081 CCGCCGCCAC CGACACCGCC GCGGGCGCGG GGGGCGCGGG GGGCGCGGGG GGCGCCGGCG 
     1141 GGCCCGGGTT CCGGCCGCTC GCGCCGCGTC CCTGGCGCTG GCTGCTGCTG CTGGCGCTGC 
     1201 CTGCCGCCTG CTCCGCGCCC CCGCCGCGCC CCGTCTACAC CAACCACTGG GCGGTGCAAG 
     1261 TGCTGGGCGG CCCGGCCGAG GCGGACCGCG TGGCGGCGGC GCACGGGTAC CTCAACTTGG 
     1321 GCCAGATTGG AAACCTGGAA GATTACTACC ATTTTTATCA CAGCAAAACC TTTAAAAGAT 
     1381 CAACCTTGAG TAGCAGAGGC CCTCACACCT TCCTCAGAAT GGACCCCCAG GTGAAATGGC 
     1441 TCCAGCAACA GGAAGTGAAA CGAAGGGTGA AGAGACAGGT GCGAAGTGAC CCGCAGGCCC 
     1501 TTTACTTCAA CGACCCCATT TGGTCCAACA TGTGGTACCT GCATTGTGGC GACAAGAACA 
     1561 GTCGCTGCCG GTCGGAAATG AATGTCCAGG CAGCGTGGAA GAGGGGCTAC ACAGGAAAAA 
     1621 ACGTGGTGGT CACCATCCTT GATGATGGCA TAGAGAGAAA TCACCCTGAC CTGGCCCCAA 
     1681 ATTATGATTC CTACGCCAGC TACGACGTGA ACGGCAATGA TTATGACCCA TCTCCACGAT 
     1741 ATGATGCCAG CAATGAAAAT AAACACGGCA CTCGTTGTGC GGGAGAAGTT GCTGCTTCAG 
     1801 CAAACAATTC CTACTGCATC GTGGGCATAG CGTACAATGC CAAAATAGGA GGCATCCGCA 
     1861 TGCTGGACGG CGATGTCACA GATGTGGTCG AGGCAAAGTC GCTGGGCATC AGACCCAACT 
     1921 ACATCGACAT TTACAGTGCC AGCTGGGGGC CGGACGACGA CGGCAAGACG GTGGACGGGC 
     1981 CCGGCCGACT GGCTAAGCAG GCTTTCGAGT ATGGCATTAA AAAGGGCCGG CAGGGCCTGG 
     2041 GCTCCATTTT CGTCTGGGCA TCTGGGAATG GCGGGAGAGA GGGGGACTAC TGCTCGTGCG 
     2101 ATGGCTACAC CAACAGCATC TACACCATCT CCGTCAGCAG CGCCACCGAG AATGGCTACA 
     2161 AGCCCTGGTA CCTGGAAGAG TGTGCCTCCA CCCTGGCCAC CACCTACAGC AGTGGGGCCT 
     2221 TTTATGAGCG AAAAATCGTC ACCACGGATC TGCGTCAGCG CTGTACCGAT GGCCACACTG 
     2281 GGACCTCAGT CTCTGCCCCC ATGGTGGCGG GCATCATCGC CTTGGCTCTA GAAGCAAACA 
     2341 GCCAGTTAAC CTGGAGGGAC GTCCAGCACC TGCTAGTGAA GACATCCCGG CCGGCCCACC 
     2401 TGAAAGCGAG CGACTGGAAA GTGAACGGCG CGGGTCATAA AGTTAGCCAT TTCTATGGAT 
     2461 TTGGTTTGGT GGACGCAGAA GCTCTCGTTG TGGAGGCAAA GAAGTGGACA GCAGTGCCAT 
     2521 CGCAGCACAT GTGTGTGGCC GCCTCGGACA AGAGACCCAG GAGCATCCCC TTAGTGCAGG 
     2581 TGCTGCGGAC TACGGCCCTG ACCAGCGCCT GCGCGGAGCA CTCGGACCAG CGGGTGGTCT 
     2641 ACTTGGAGCA CGTGGTGGTT CGCACCTCCA TCTCACACCC ACGCCGAGGA GACCTCCAGA 
     2701 TCTACCTGGT TTCTCCCTCG GGAACCAAGT CTCAACTTCT GGCAAAGAGG TTGCTGGATC 
     2761 TTTCCAATGA AGGGTTTACA AACTGGGAAT TCATGACTGT CCACTGCTGG GGAGAAAAGG 
     2821 CTGAAGGGCA GTGGACCTTG GAAATCCAAG ATCTGCCATC CCAGGTCCGC AACCCGGAGA 
     2881 AGCAAGGGAA GTTGAAAGAA TGGAGCCTCA TACTGTATGG CACAGCAGAG CACCCGTACC 
     2941 ACACCTTCAG TGCCCATCAG TCCCGCTCGC GGATGCTGGA GCTCTCAGCC CCAGAGCTGG 
     3001 AGCCACCCAA GGCTGCCCTG TCACCCTCCC AGGTGGAAGT TCCTGAAGAT GAGGAAGATT 
     3061 ACACAGGTGT GTGCCATCCG GAGTGTGGTG ACAAAGGCTG TGATGGCCCC AATGCAGACC 
     3121 AGTGCTTGAA CTGCGTCCAC TTCAGCCTGG GGAGTGTCAA GACCAGCAGG AAGTGCGTGA 
     3181 GTGTGTGCCC CTTGGGCTAC TTTGGGGACA CAGCAGCAAG ACGCTGTCGC CGGTGCCACA 
     3241 AGGGGTGTGA GACCTGCTCC AGCAGAGCTG CGACGCAGTG CCTGTCTTGC CGCCGCGGGT 
     3301 TCTATCACCA CCAGGAGATG AACACCTGTG TGACCCTCTG TCCTGCAGGA TTTTATGCTG 
     3361 ATGAAAGTCA GAAAAATTGC CTTAAATGCC ACCCAAGCTG TAAAAAGTGC GTGGATGAAC 
     3421 CTGAGAAATG TACTGTCTGT AAAGAAGGAT TCAGCCTTGC ACGGGGCAGC TGCATTCCTG 
     3481 ACTGTGAGCC AGGCACCTAC TTTGACTCAG AGCTGATCAG ATGTGGGGAA TGCCATCACA 
     3541 CCTGCGGAAC CTGCGTGGGG CCAGGCAGAG AAGAGTGCAT TCACTGTGCG AAAAACTTCC 
     3601 ACTTCCACGA CTGGAAGTGT GTGCCAGCCT GTGGTGAGGG CTTCTACCCA GAAGAGATGC 
     3661 CGGGCTTGCC CCACAAAGTG TGTCGAAGGT ACGGTCCTCC TGGCGGGGAA CGGCAGGCAG 
     3721 CTGTGTCCAG CAAAGGAGTG CCTGGAGGGC AGAGTCTGGC AGCATCTTCC CCAGGGGCCG 
     3781 GGGAGGGCAT GTTGCATCAC CCCACTGTGG ACAGGAGCCC ATTTACAGAG CTGCTGAGGG 
     3841 GCCTTCGTCC CTTTGTTCAC TGGATGCATA TTTGTTGGGT CCCTGCTGTG GGCAGACACC 
     3901 GTGCAGCAGC GGGAACGCGT ACGCGGCCGC TCGAGATGGA GAGCGACGAG AGCGGCCTGC 
     3961 CCGCCATGGA GATCGAGTGC CGCATCACCG GCACCCTGAA CGGCGTGGAG TTCGAGCTGG 
     4021 TGGGCGGCGG AGAGGGCACC CCCGAGCAGG GCCGCATGAC CAACAAGATG AAGAGCACCA 
     4081 AAGGCGCCCT GACCTTCAGC CCCTACCTGC TGAGCCACGT GATGGGCTAC GGCTTCTACC 
     4141 ACTTCGGCAC CTACCCCAGC GGCTACGAGA ACCCCTTCCT GCACGCCATC AACAACGGCG 
     4201 GCTACACCAA CACCCGCATC GAGAAGTACG AGGACGGCGG CGTGCTGCAC GTGAGCTTCA 
     4261 GCTACCGCTA CGAGGCCGGC CGCGTGATCG GCGACTTCAA GGTGATGGGC ACCGGCTTCC 
     4321 CCGAGGACAG CGTGATCTTC ACCGACAAGA TCATCCGCAG CAACGCCACC GTGGAGCACC 
     4381 TGCACCCCAT GGGCGATAAC GATCTGGATG GCAGCTTCAC CCGCACCTTC AGCCTGCGCG 
     4441 ACGGCGGCTA CTACAGCTCC GTGGTGGACA GCCACATGCA CTTCAAGAGC GCCATCCACC 
     4501 CCAGCATCCT GCAGAACGGG GGCCCCATGT TCGCCTTCCG CCGCGTGGAG GAGGATCACA 
     4561 GCAACACCGA GCTGGGCATC GTGGAGTACC AGCACGCCTT CAAGACCCCG GATGCAGATG 
     4621 CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG 
     5341 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5401 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5461 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 
     5521 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 
     5581 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 
     5641 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 
     5701 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 
     5761 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 
     5821 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 
     5881 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 
     5941 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 
     6001 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 
     6061 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 
     6121 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 
     6181 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 
     6241 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 
     6301 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 
     6361 CTGGGTGTGG CCGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 
     6421 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 
     6481 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 
     6541 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 
     6601 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 
     6661 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 
     6721 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 
     6781 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 
     6841 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 
     6901 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 
     6961 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 
     7021 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 
     7081 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 
     7141 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     7201 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7261 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7321 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7381 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7441 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7501 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7561 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7621 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     7681 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     7741 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     7801 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 
     7861 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 
     7921 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 
     7981 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 
     8041 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 
     8101 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 
     8161 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 
     8221 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 
     8281 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 
     8341 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 
     8401 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 
     8461 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 
     8521 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 
     8581 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 
     8641 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 
     8701 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 
     8761 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 
     8821 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 
     8881 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 
     8941 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 
     9001 GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 
     9061 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 
     9121 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 
     9181 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 
     9241 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 
     9301 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 
     9361 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 
     9421 TTTAACAAAA ATTTAACGCG AATTTT
//