LOCUS       RG217077                9428 bp    DNA     circular  SYN 
DEFINITION  PCSK6 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 2
ACCESSION   NM_138319
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3909..4613 
                     /label="GFP"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    7337..7982 
                     /label="ColE1"  
     misc_feature    5699..6493 
                     /label="Neo"  
     misc_feature    5330..5632 
                     /label="SV40 ori"  
     misc_feature    7997..8857 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3888..3894 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3888 
                     /label="PCSK6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCGCGC GCGCCGCCTG CGCCCGGGCC CCGGCCGCCG CCCCGGGCCG 
     1081 CCGCCGCCAC CGACACCGCC GCGGGCGCGG GGGGCGCGGG GGGCGCGGGG GGCGCCGGCG 
     1141 GGCCCGGGTT CCGGCCGCTC GCGCCGCGTC CCTGGCGCTG GCTGCTGCTG CTGGCGCTGC 
     1201 CTGCCGCCTG CTCCGCGCCC CCGCCGCGCC CCGTCTACAC CAACCACTGG GCGGTGCAAG 
     1261 TGCTGGGCGG CCCGGCCGAG GCGGACCGCG TGGCGGCGGC GCACGGGTAC CTCAACTTGG 
     1321 GCCAGATTGG AAACCTGGAA GATTACTACC ATTTTTATCA CAGCAAAACC TTTAAAAGAT 
     1381 CAACCTTGAG TAGCAGAGGC CCTCACACCT TCCTCAGAAT GGACCCCCAG GTGAAATGGC 
     1441 TCCAGCAACA GGAAGTGAAA CGAAGGGTGA AGAGACAGGT GCGAAGTGAC CCGCAGGCCC 
     1501 TTTACTTCAA CGACCCCATT TGGTCCAACA TGTGGTACCT GCATTGTGGC GACAAGAACA 
     1561 GTCGCTGCCG GTCGGAAATG AATGTCCAGG CAGCGTGGAA GAGGGGCTAC ACAGGAAAAA 
     1621 ACGTGGTGGT CACCATCCTT GATGATGGCA TAGAGAGAAA TCACCCTGAC CTGGCCCCAA 
     1681 ATTATGATTC CTACGCCAGC TACGACGTGA ACGGCAATGA TTATGACCCA TCTCCACGAT 
     1741 ATGATGCCAG CAATGAAAAT AAACACGGCA CTCGTTGTGC GGGAGAAGTT GCTGCTTCAG 
     1801 CAAACAATTC CTACTGCATC GTGGGCATAG CGTACAATGC CAAAATAGGA GGCATCCGCA 
     1861 TGCTGGACGG CGATGTCACA GATGTGGTCG AGGCAAAGTC GCTGGGCATC AGACCCAACT 
     1921 ACATCGACAT TTACAGTGCC AGCTGGGGGC CGGACGACGA CGGCAAGACG GTGGACGGGC 
     1981 CCGGCCGACT GGCTAAGCAG GCTTTCGAGT ATGGCATTAA AAAGGGCCGG CAGGGCCTGG 
     2041 GCTCCATTTT CGTCTGGGCA TCTGGGAATG GCGGGAGAGA GGGGGACTAC TGCTCGTGCG 
     2101 ATGGCTACAC CAACAGCATC TACACCATCT CCGTCAGCAG CGCCACCGAG AATGGCTACA 
     2161 AGCCCTGGTA CCTGGAAGAG TGTGCCTCCA CCCTGGCCAC CACCTACAGC AGTGGGGCCT 
     2221 TTTATGAGCG AAAAATCGTC ACCACGGATC TGCGTCAGCG CTGTACCGAT GGCCACACTG 
     2281 GGACCTCAGT CTCTGCCCCC ATGGTGGCGG GCATCATCGC CTTGGCTCTA GAAGCAAACA 
     2341 GCCAGTTAAC CTGGAGGGAC GTCCAGCACC TGCTAGTGAA GACATCCCGG CCGGCCCACC 
     2401 TGAAAGCGAG CGACTGGAAA GTGAACGGCG CGGGTCATAA AGTTAGCCAT TTCTATGGAT 
     2461 TTGGTTTGGT GGACGCAGAA GCTCTCGTTG TGGAGGCAAA GAAGTGGACA GCAGTGCCAT 
     2521 CGCAGCACAT GTGTGTGGCC GCCTCGGACA AGAGACCCAG GAGCATCCCC TTAGTGCAGG 
     2581 TGCTGCGGAC TACGGCCCTG ACCAGCGCCT GCGCGGAGCA CTCGGACCAG CGGGTGGTCT 
     2641 ACTTGGAGCA CGTGGTGGTT CGCACCTCCA TCTCACACCC ACGCCGAGGA GACCTCCAGA 
     2701 TCTACCTGGT TTCTCCCTCG GGAACCAAGT CTCAACTTCT GGCAAAGAGG TTGCTGGATC 
     2761 TTTCCAATGA AGGGTTTACA AACTGGGAAT TCATGACTGT CCACTGCTGG GGAGAAAAGG 
     2821 CTGAAGGGCA GTGGACCTTG GAAATCCAAG ATCTGCCATC CCAGGTCCGC AACCCGGAGA 
     2881 AGCAAGGGAA GTTGAAAGAA TGGAGCCTCA TACTGTATGG CACAGCAGAG CACCCGTACC 
     2941 ACACCTTCAG TGCCCATCAG TCCCGCTCGC GGATGCTGGA GCTCTCAGCC CCAGAGCTGG 
     3001 AGCCACCCAA GGCTGCCCTG TCACCCTCCC AGGTGGAAGT TCCTGAAGAT GAGGAAGATT 
     3061 ACACAGGTGT GTGCCATCCG GAGTGTGGTG ACAAAGGCTG TGATGGCCCC AATGCAGACC 
     3121 AGTGCTTGAA CTGCGTCCAC TTCAGCCTGG GGAGTGTCAA GACCAGCAGG AAGTGCGTGA 
     3181 GTGTGTGCCC CTTGGGCTAC TTTGGGGACA CAGCAGCAAG ACGCTGTCGC CGGTGCCACA 
     3241 AGGGGTGTGA GACCTGCTCC AGCAGAGCTG CGACGCAGTG CCTGTCTTGC CGCCGCGGGT 
     3301 TCTATCACCA CCAGGAGATG AACACCTGTG TGACCCTCTG TCCTGCAGGA TTTTATGCTG 
     3361 ATGAAAGTCA GAAAAATTGC CTTAAATGCC ACCCAAGCTG TAAAAAGTGC GTGGATGAAC 
     3421 CTGAGAAATG TACTGTCTGT AAAGAAGGAT TCAGCCTTGC ACGGGGCAGC TGCATTCCTG 
     3481 ACTGTGAGCC AGGCACCTAC TTTGACTCAG AGCTGATCAG ATGTGGGGAA TGCCATCACA 
     3541 CCTGCGGAAC CTGCGTGGGG CCAGGCAGAG AAGAGTGCAT TCACTGTGCG AAAAACTTCC 
     3601 ACTTCCACGA CTGGAAGTGT GTGCCAGCCT GTGGTGAGGG CTTCTACCCA GAAGAGATGC 
     3661 CGGGCTTGCC CCACAAAGTG TGTCGAAGGT GTGACGAGAA CTGCTTGAGC TGTGCAGGCT 
     3721 CCAGCAGGAA CTGTAGCAGG TGTAAGACGG GCTTCACACA GCTGGGGACC TCCTGCATCA 
     3781 CCAACCACAC GTGCAGCAAC GCTGACGAGA CATTCTGCGA GATGGTGAAG TCCAACCGGC 
     3841 TGTGCGAACG GAAGCTCTTC ATTCAGTTCT GCTGCCGCAC GTGCCTCCTG GCCGGGACGC 
     3901 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3961 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4021 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4081 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4141 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4201 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4261 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4321 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4381 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4441 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4501 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4561 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5341 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5401 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5461 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5521 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5581 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5641 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5701 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5761 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5821 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5881 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5941 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6001 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6061 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6121 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6181 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6241 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6301 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6361 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6421 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6481 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6541 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7021 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7081 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7141 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7201 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7261 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7321 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7381 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7441 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7501 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7561 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7621 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7681 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7741 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9001 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9061 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9121 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9181 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9241 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9301 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9361 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9421 CGAATTTT
//