LOCUS       RG216572                9518 bp    DNA     circular  SYN 
DEFINITION  BMP1 (tGFP-tagged) - Human bone morphogenetic protein 1 (BMP1), transcript variant 3
ACCESSION   NM_006129
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3999..4703 
                     /label="GFP"  
     misc_feature    4750..5335 
                     /label="PolyA signal"  
     misc_feature    7427..8072 
                     /label="ColE1"  
     misc_feature    5789..6583 
                     /label="Neo"  
     misc_feature    5420..5722 
                     /label="SV40 ori"  
     misc_feature    8087..8947 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3978..3984 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3978 
                     /label="BMP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGCGTG GCCCGCCTGC CGCTGCTGCT CGGGCTGCTG CTGCTCCCGC 
     1081 GTCCCGGCCG GCCGCTGGAC TTGGCCGACT ACACCTATGA CCTGGCGGAG GAGGACGACT 
     1141 CGGAGCCCCT CAACTACAAA GACCCCTGCA AGGCGGCTGC CTTTCTTGGG GACATTGCCC 
     1201 TGGACGAAGA GGACCTGAGG GCCTTCCAGG TACAGCAGGC TGTGGATCTC AGACGGCACA 
     1261 CAGCTCGTAA GTCCTCCATC AAAGCTGCAG TTCCAGGAAA CACTTCTACC CCCAGCTGCC 
     1321 AGAGCACCAA CGGGCAGCCT CAGAGGGGAG CCTGTGGGAG ATGGAGAGGT AGATCCCGTA 
     1381 GCCGGCGGGC GGCGACGTCC CGACCAGAGC GTGTGTGGCC CGATGGGGTC ATCCCCTTTG 
     1441 TCATTGGGGG AAACTTCACT GGTAGCCAGA GGGCAGTCTT CCGGCAGGCC ATGAGGCACT 
     1501 GGGAGAAGCA CACCTGTGTC ACCTTCCTGG AGCGCACTGA CGAGGACAGC TATATTGTGT 
     1561 TCACCTATCG ACCTTGCGGG TGCTGCTCCT ACGTGGGTCG CCGCGGCGGG GGCCCCCAGG 
     1621 CCATCTCCAT CGGCAAGAAC TGTGACAAGT TCGGCATTGT GGTCCACGAG CTGGGCCACG 
     1681 TCGTCGGCTT CTGGCACGAA CACACTCGGC CAGACCGGGA CCGCCACGTT TCCATCGTTC 
     1741 GTGAGAACAT CCAGCCAGGG CAGGAGTATA ACTTCCTGAA GATGGAGCCT CAGGAGGTGG 
     1801 AGTCCCTGGG GGAGACCTAT GACTTCGACA GCATCATGCA TTACGCTCGG AACACATTCT 
     1861 CCAGGGGCAT CTTCCTGGAT ACCATTGTCC CCAAGTATGA GGTGAACGGG GTGAAACCTC 
     1921 CCATTGGCCA AAGGACACGG CTCAGCAAGG GGGACATTGC CCAAGCCCGC AAGCTTTACA 
     1981 AGTGCCCAGC CTGTGGAGAG ACCCTGCAAG ACAGCACAGG CAACTTCTCC TCCCCTGAAT 
     2041 ACCCCAATGG CTACTCTGCT CACATGCACT GCGTGTGGCG CATCTCTGTC ACACCCGGGG 
     2101 AGAAGATCAT CCTGAACTTC ACGTCCCTGG ACCTGTACCG CAGCCGCCTG TGCTGGTACG 
     2161 ACTATGTGGA GGTCCGAGAT GGCTTCTGGA GGAAGGCGCC CCTCCGAGGC CGCTTCTGCG 
     2221 GGTCCAAACT CCCTGAGCCT ATCGTCTCCA CTGACAGCCG CCTCTGGGTT GAATTCCGCA 
     2281 GCAGCAGCAA TTGGGTTGGA AAGGGCTTCT TTGCAGTCTA CGAAGCCATC TGCGGGGGTG 
     2341 ATGTGAAAAA GGACTATGGC CACATTCAAT CGCCCAACTA CCCAGACGAT TACCGGCCCA 
     2401 GCAAAGTCTG CATCTGGCGG ATCCAGGTGT CTGAGGGCTT CCACGTGGGC CTCACATTCC 
     2461 AGTCCTTTGA GATTGAGCGC CACGACAGCT GTGCCTACGA CTATCTGGAG GTGCGCGACG 
     2521 GGCACAGTGA GAGCAGCACC CTCATCGGGC GCTACTGTGG CTATGAGAAG CCTGATGACA 
     2581 TCAAGAGCAC GTCCAGCCGC CTCTGGCTCA AGTTCGTCTC TGACGGGTCC ATTAACAAAG 
     2641 CGGGCTTTGC CGTCAACTTT TTCAAAGAGG TGGACGAGTG CTCTCGGCCC AACCGCGGGG 
     2701 GCTGTGAGCA GCGGTGCCTC AACACCCTGG GCAGCTACAA GTGCAGCTGT GACCCCGGGT 
     2761 ACGAGCTGGC CCCAGACAAG CGCCGCTGTG AGGCTGCTTG TGGCGGATTC CTCACCAAGC 
     2821 TCAACGGCTC CATCACCAGC CCGGGCTGGC CCAAGGAGTA CCCCCCCAAC AAGAACTGCA 
     2881 TCTGGCAGCT GGTGGCCCCC ACCCAGTACC GCATCTCCCT GCAGTTTGAC TTCTTTGAGA 
     2941 CAGAGGGCAA TGATGTGTGC AAGTACGACT TCGTGGAGGT GCGCAGTGGA CTCACAGCTG 
     3001 ACTCCAAGCT GCATGGCAAG TTCTGTGGTT CTGAGAAGCC CGAGGTCATC ACCTCCCAGT 
     3061 ACAACAACAT GCGCGTGGAG TTCAAGTCCG ACAACACCGT GTCCAAAAAG GGCTTCAAGG 
     3121 CCCACTTCTT CTCAGACAAG GACGAGTGCT CCAAGGATAA CGGCGGCTGC CAGCAGGACT 
     3181 GCGTCAACAC GTTCGGCAGT TATGAGTGCC AATGCCGCAG TGGCTTCGTC CTCCATGACA 
     3241 ACAAGCACGA CTGCAAAGAA GCCGGCTGTG ACCACAAGGT GACATCCACC AGTGGTACCA 
     3301 TCACCAGCCC CAACTGGCCT GACAAGTATC CCAGCAAGAA GGAGTGCACG TGGGCCATCT 
     3361 CCAGCACCCC CGGGCACCGG GTCAAGCTGA CCTTCATGGA GATGGACATC GAGTCCCAGC 
     3421 CTGAGTGTGC CTACGACCAC CTAGAGGTGT TCGACGGGCG AGACGCCAAG GCCCCCGTCC 
     3481 TCGGCCGCTT CTGTGGGAGC AAGAAGCCCG AGCCCGTCCT GGCCACAGGC AGCCGCATGT 
     3541 TCCTGCGCTT CTACTCAGAT AACTCGGTCC AGCGAAAGGG CTTCCAGGCC TCCCACGCCA 
     3601 CAGAGTGCGG GGGCCAGGTA CGGGCAGACG TGAAGACCAA GGACCTTTAC TCCCACGCCC 
     3661 AGTTTGGCGA CAACAACTAC CCTGGGGGTG TGGACTGTGA GTGGGTCATT GTGGCCGAGG 
     3721 AAGGCTACGG CGTGGAGCTC GTGTTCCAGA CCTTTGAGGT GGAGGAGGAG ACCGACTGCG 
     3781 GCTATGACTA CATGGAGCTC TTCGACGGCT ACGACAGCAC AGCCCCCAGG CTGGGGCGCT 
     3841 ACTGTGGCTC AGGGCCTCCT GAGGAGGTGT ACTCGGCGGG AGATTCTGTC CTGGTGAAGT 
     3901 TCCACTCGGA TGACACCATC ACCAAAAAAG GTTTCCACCT GCGATACACC AGCACCAAGT 
     3961 TCCAGGACAC ACTCCACAGC AGGAAGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     4021 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     4081 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     4141 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     4201 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     4261 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     4321 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     4381 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     4441 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     4501 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4561 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4621 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4681 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     5401 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     5461 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5521 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5581 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5641 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5701 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5761 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5821 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5881 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5941 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     6001 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     6061 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     6121 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     6181 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     6241 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     6301 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     6361 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     6421 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     6481 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6541 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6601 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6661 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6721 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6781 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6841 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6901 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6961 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     7021 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     7081 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     7141 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     7201 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     7261 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     7321 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     7381 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     7441 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     7501 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7561 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7621 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7681 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7741 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7801 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7861 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7921 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7981 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     8041 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     8101 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     8161 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     8221 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     8281 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     8341 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     8401 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     8461 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     8521 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8581 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8641 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8701 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8761 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8821 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8881 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8941 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     9001 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     9061 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     9121 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     9181 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     9241 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     9301 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     9361 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     9421 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     9481 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//