LOCUS       RG216399                9404 bp    DNA     circular  SYN 
DEFINITION  ERC1 (tGFP-tagged) - Human ELKS/RAB6-interacting/CAST family member 1 (ERC1), transcript variant alpha
ACCESSION   NM_015064
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3885..4589 
                     /label="GFP"  
     misc_feature    4636..5221 
                     /label="PolyA signal"  
     misc_feature    7313..7958 
                     /label="ColE1"  
     misc_feature    5675..6469 
                     /label="Neo"  
     misc_feature    5306..5608 
                     /label="SV40 ori"  
     misc_feature    7973..8833 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3864..3870 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3864 
                     /label="ERC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGGAAGT GCCCGCTCTG TTGGGAAGGT GGAGCCGAGC AGCCAGAGCC 
     1081 CTGGGCGTTC ACCCAGGCTT CCACGTTCCC CTCGCTTGGG TCACCGTCGA ACCAACAGTA 
     1141 CGGGAGGGAG TTCGGGAAGC AGTGTTGGAG GTGGCAGTGG GAAAACCCTT TCAATGGAAA 
     1201 ATATACAATC TTTAAATGCT GCCTATGCCA CCTCTGGCCC TATGTATCTA AGTGACCATG 
     1261 AAAATGTGGG TTCAGAAACA CCTAAAAGCA CCATGACACT TGGCCGTTCT GGGGGACGTC 
     1321 TGCCTTACGG TGTTCGGATG ACTGCTATGG GTAGTAGCCC CAATATAGCT AGCAGTGGGG 
     1381 TTGCTAGTGA CACCATAGCA TTTGGAGAGC ATCACCTCCC TCCTGTGAGT ATGGCATCCA 
     1441 CTGTACCTCA CTCCCTTCGT CAGGCGAGAG ATAACACAAT CATGGATCTG CAGACACAGC 
     1501 TGAAGGAAGT ATTAAGAGAA AATGATCTCT TGCGGAAGGA TGTGGAAGTA AAGGAGAGCA 
     1561 AATTGAGTTC TTCAATGAAT AGCATCAAGA CCTTCTGGAG CCCAGAGCTG AAGAAGGAAC 
     1621 GAGCCCTGAG AAAAGATGAA GCTTCCAAAA TCACCATTTG GAAGGAACAG TACAGAGTTG 
     1681 TACAGGAGGA AAACCAGCAC ATGCAGATGA CAATCCAGGC TCTCCAGGAT GAATTGCGGA 
     1741 TCCAGAGGGA CCTGAATCAG CTGTTTCAGC AGGATAGTAG CAGCAGGACT GGCGAACCTT 
     1801 GTGTAGCAGA GCTGACAGAG GAGAACTTTC AGAGGCTTCA TGCTGAGCAT GAGCGGCAGG 
     1861 CCAAAGAGCT GTTTCTTCTT CGAAAGACAT TGGAGGAAAT GGAGCTGCGT ATTGAGACTC 
     1921 AAAAGCAGAC CCTAAATGCT CGGGATGAAT CCATTAAGAA GCTTCTGGAA ATGTTGCAGA 
     1981 GCAAAGGACT TTCTGCCAAG GCTACCGAGG AAGACCATGA GAGAACAAGA CGACTGGCAG 
     2041 AGGCAGAGAT GCACGTTCAT CACCTAGAAA GCCTTTTGGA GCAGAAGGAA AAAGAGAACA 
     2101 GTATGTTGAG AGAGGAGATG CATCGAAGGT TTGAGAATGC TCCTGATTCT GCCAAAACAA 
     2161 AAGCTCTGCA AACTGTTATT GAGATGAAGG ATTCAAAAAT TTCCTCTATG GAGCGTGGGC 
     2221 TTCGAGACCT GGAAGAGGAA ATTCAGATGC TGAAATCGAA TGGTGCTTTG AGTACTGAGG 
     2281 AAAGGGAAGA AGAAATGAAG CAAATGGAAG TGTATCGGAG CCATTCTAAA TTTATGAAAA 
     2341 ATAAGATTGG CCAGGTGAAA CAGGAGCTGT CCAGAAAGGA CACAGAACTA CTCGCCCTGC 
     2401 AGACAAAGCT AGAAACACTC ACAAACCAGT TCTCAGATAG TAAACAGCAC ATTGAAGTGT 
     2461 TGAAGGAGTC CTTGACTGCT AAGGAGCAGA GGGCTGCCAT CCTGCAGACT GAGGTGGATG 
     2521 CTCTCCGATT GCGTTTGGAA GAGAAGGAAA CCATGTTGAA TAAAAAGACA AAACAAATTC 
     2581 AGGATATGGC TGAAGAGAAG GGGACACAAG CTGGAGAGAT ACATGACCTC AAGGACATGT 
     2641 TGGATGTGAA GGAGCGGAAG GTTAATGTTC TTCAGAAGAA GATTGAAAAT CTTCAAGAGC 
     2701 AGCTTAGAGA CAAGGAAAAG CAGATGAGCA GCTTGAAAGA ACGGGTCAAA TCCTTGCAGG 
     2761 CTGACACCAC CAACACTGAC ACTGCCTTGA CAACTTTGGA GGAGGCCCTT GCAGAGAAAG 
     2821 AGCGGACAAT TGAACGCTTA AAGGAGCAGA GGGACAGAGA TGAGCGAGAG AAGCAAGAGG 
     2881 AAATTGATAA CTACAAAAAA GATCTTAAAG ACTTGAAGGA AAAAGTCAGC CTGTTGCAAG 
     2941 GCGACCTTTC AGAGAAAGAG GCTTCACTTT TGGATCTGAA AGAGCATGCT TCTTCTCTGG 
     3001 CATCCTCAGG ACTGAAAAAG GACTCACGGC TTAAGACACT AGAGATTGCT TTGGAGCAGA 
     3061 AGAAGGAGGA GTGTCTGAAA ATGGAATCAC AATTGAAAAA GGCACATGAG GCAGCATTGG 
     3121 AAGCCAGAGC CAGTCCAGAG ATGAGTGACC GAATACAGCA CTTGGAGAGA GAGATCACCA 
     3181 GGTACAAAGA TGAATCTAGC AAGGCCCAGG CAGAAGTTGA TCGACTCTTA GAAATCTTGA 
     3241 AGGAGGTGGA AAATGAGAAG AATGACAAAG ATAAGAAGAT AGCTGAGTTG GAAAGTCTCA 
     3301 CCTCAAGGCA AGTGAAAGAC CAGAATAAGA AGGTAGCAAA TCTGAAGCAC AAGGAACAGG 
     3361 TGGAAAAAAA GAAGAGTGCA CAAATGTTAG AGGAGGCGCG ACGACGGGAG GACAATCTCA 
     3421 ACGACAGCTC TCAGCAGCTA CAGGTGGAGG AGTTACTGAT GGCCATGGAG AAGGTAAAGC 
     3481 AGGAACTAGA ATCCATGAAA GCAAAGCTGT CCTCCACCCA GCAGTCTCTG GCAGAAAAGG 
     3541 AAACTCACTT GACTAATCTT CGGGCAGAGA GAAGGAAACA CTTAGAGGAA GTTCTGGAGA 
     3601 TGAAGCAAGA AGCTCTTCTG GCTGCCATTA GTGAAAAAGA CGCCAATATA GCTCTCTTGG 
     3661 AGCTTTCGTC CTCTAAGAAG AAGACCCAAG AGGAAGTGGC TGCCCTGAAG CGGGAGAAGG 
     3721 ATCGTCTGGT ACAGCAGCTT AAGCAGCAGA CGCAAAATCG AATGAAGCTA ATGGCCGACA 
     3781 ACTACGAGGA TGACCACTTC AAATCCTCCC ATTCCAATCA AACAAATCAC AAGCCCTCCC 
     3841 CAGACCAGGA TGAGGAGGAG GGTATATGGG CAACGCGTAC GCGGCCGCTC GAGATGGAGA 
     3901 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     3961 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     4021 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4081 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4141 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4201 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4261 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4321 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4381 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4441 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4501 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4561 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4621 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4681 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4741 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4801 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4861 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4921 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4981 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5041 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5101 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5161 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5221 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5281 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5341 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5401 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5461 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5521 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5581 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5641 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5701 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5761 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     5821 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     5881 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     5941 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     6001 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6061 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6121 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6181 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6241 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6301 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6361 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6421 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6481 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6541 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6601 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6661 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6721 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6781 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     6841 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     6901 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     6961 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     7021 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7081 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7141 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7201 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7261 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7321 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7381 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7441 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7501 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7561 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7621 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7681 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7741 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7801 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     7861 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     7921 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     7981 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     8041 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8101 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8161 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8221 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8281 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8341 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8401 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8461 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8521 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8581 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8641 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8701 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8761 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     8821 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     8881 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     8941 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     9001 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9061 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9121 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9181 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9241 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9301 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9361 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//