LOCUS       RG216017                8978 bp    DNA     circular  SYN 
DEFINITION  PCDHA6 (tGFP-tagged) - Human protocadherin alpha 6 (PCDHA6), transcript variant 2
ACCESSION   NM_031848
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3457..4161 
                     /label="GFP"  
     misc_feature    4208..4793 
                     /label="PolyA signal"  
     misc_feature    6885..7530 
                     /label="ColE1"  
     misc_feature    5247..6041 
                     /label="Neo"  
     misc_feature    4878..5180 
                     /label="SV40 ori"  
     misc_feature    7545..8405 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3429..3436 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3429 
                     /label="PCDHA6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGTTTACC CCGGAGGATA GATTGGGAAA GCAATGTCTG CTCCTCCCGC 
     1081 TTCTGCTCCT CGCAGCCTGG AAGGTGGGGA GCGGCCAGCT CCACTACTCC GTACCCGAGG 
     1141 AGGCCAAACA CGGCACCTTC GTGGGCCGGA TCGCGCAGGA CCTGGGGCTG GAGCTGGCGG 
     1201 AGCTGGTGCC GCGCCTGTTC AGGATGGCCT CCAAAGACCG CGAGGACCTT CTGGAGGTAA 
     1261 ATCTGCAGAA TGGCATTTTG TTTGTGAATT CTCGGATCGA CCGCGAGGAG CTGTGCGGGC 
     1321 GGAGCGCGGA GTGCAGCATC CACCTGGAGG TGATCGTGGA CAGGCCGCTG CAGGTTTTCC 
     1381 ATGTGGACGT GGAGGTGAGG GACATTAACG ACAACCCGCC CTTGTTCCCG GTAGAGGAAC 
     1441 AAAGAGTGCT GATTTACGAA TCTAGGCTGC CAGATTCTGT GTTTCCACTG GAGGGCGCGT 
     1501 CCGATGCAGA TGTTGGCTCA AATTCCATCT TAACCTATAA ACTCAGTTCT AGCGAATACT 
     1561 TCGGGCTAGA TGTGAAAATA AACAGTGATG ACAATAAACA AATTGGGCTC TTATTAAAGA 
     1621 AATCCTTGGA CAGAGAGGAA GCTCCTGCAC ACAACTTATT CCTGACAGCC ACAGATGGGG 
     1681 GCAAACCTGA GCTCACAGGC ACTGTTCAGC TGCTGGTCAC AGTGCTGGAT GTGAATGATA 
     1741 ATGCTCCCAC TTTCGAACAG TCTGAATACG AAGTAAGAAT ATTCGAAAAT GCAGACAACG 
     1801 GAACAACAGT TATCAGACTG AATGCTTCTG ATCGGGATGA AGGAGCGAAT GGGGCAATTT 
     1861 CATATTCTTT TAATAGCCTT GTTGCAGCCA TGGTTATTGA CCACTTTAGC ATAGATCGAA 
     1921 ATACGGGAGA AATAGTGATT CGGGGTAATT TGGATTTTGA ACAAGAAAAC TTATACAAAA 
     1981 TCCTCATTGA CGCCACGGAC AAAGGCCATC CTCCCATGGC GGGTCATTGC ACCGTTTTAG 
     2041 TGAGAATTTT GGATAAAAAT GATAACGTCC CTGAGATAGC ACTGACTTCC TTATCCTTGC 
     2101 CTGTACGTGA AGACGCTCAA TTTGGTACTG TCATCGCCCT AATTAGCGTG AACGACCTCG 
     2161 ATTCAGGTGC CAACGGGCAG GTGAACTGCT CGCTGACGCC TCACGTCCCT TTCAAGCTGG 
     2221 TGTCCACCTT CAAGAATTAC TACTCGTTGG TGCTGGACAG TGCCCTGGAC CGCGAGAGCG 
     2281 TGTCGGCCTA TGAGTTGGTG GTAACCGCGC GGGACGGGGG CTCGCCTTCG CTGTGGGCCA 
     2341 CCGCCAGCTT GTCTGTGGAG GTGGCCGACA TGAATGACAA TGCTCCGGCG TTCGCGCAGC 
     2401 CCGAGTACAC AGTGTTCGTG AAGGAGAACA ACCCGCCGGG CTGCCACATC TTCACGGTGT 
     2461 CTGCGCGAGA CGCGGACGCG CAGGAGAACG CGCTGGTGTC CTACTCGCTG GTGGAGCGGC 
     2521 GGGTGGGCGA GCGCGCGTTG TCGAGCTACA TTTCGGTGCA CGCGGAGAGC GGCAAGGTGT 
     2581 ACGCGCTGCA GCCGCTGGAC CACGAGGAGC TAGAGCTGCT GCAGTTTCAG GTGAGCGCGC 
     2641 GCGACGCGGG CGTGCCGCCT CTGGGCAGCA ACGTGACGCT GCAGGTGTTC GTGCTGGACG 
     2701 AGAACGACAA CGCGCCGGCG CTGCTGGCGC CTCGGGTGGG TGGTACTGGT GGTGCAGTGA 
     2761 GCGAGCTGGT GCCGCGGTCA CTGGGTGCAG GCCAAGTGGT GGCGAAGGTG CGCGCAGTTG 
     2821 ACGCCGACTC AGGCTACAAC GCGTGGCTTT CGTATGAGCT GCAGCCCCCG GCAAGCAGCG 
     2881 CTCGCTTCCC GTTTCGCGTG GGGCTGTACA CGGGCGAGAT CAGCACCACT CGTGTCCTGG 
     2941 ACGAAGCGGA CTCTCCGCGC CACCGGCTGC TGGTGCTGGT GAAAGACCAC GGTGAGCCGG 
     3001 CGCTGACAGC GACGGCCACG GTTCTGGTGT CGCTGGTGGA GAGTGGCCAG GCTCCAAAGG 
     3061 CGTCATCACG GGCGTCGGTG GGCGCCGCGG GCCCAGAGGC GGCGCTGGTG GATGTCAACG 
     3121 TGTACCTGAT CATCGCCATC TGCGCGGTAT CCAGCCTGCT GGTCCTCACG CTACTGCTGT 
     3181 ACACAGCGCT GCGGTGCTCG GCGCCACCCA CCGAGGGCGC GTGCACGGCG GACAAGCCCA 
     3241 CGCTGGTGTG CTCCAGCGCA GTGGGGAGCT GGTCGTACTC GCAGCAGAGG CGGCAGAGGG 
     3301 TGTGCTCCGG GGAGGGCCCA CCCAAGATGG ATCTCATGGC CTTTAGCCCC AGCCTTTCAC 
     3361 CTTGTCCTAT TATGATGGGT AAGGCGGAGA ATCAGGATTT AAATGAAGAT CATGATGCCA 
     3421 AAGTAAGTGA ATTTTCAAGC GGACCGACGC GTACGCGGCC GCTCGAGATG GAGAGCGACG 
     3481 AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC CGGCACCCTG AACGGCGTGG 
     3541 AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA GGGCCGCATG ACCAACAAGA 
     3601 TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT GCTGAGCCAC GTGATGGGCT 
     3661 ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA GAACCCCTTC CTGCACGCCA 
     3721 TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA CGAGGACGGC GGCGTGCTGC 
     3781 ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT CGGCGACTTC AAGGTGATGG 
     3841 GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA GATCATCCGC AGCAACGCCA 
     3901 CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA TGGCAGCTTC ACCCGCACCT 
     3961 TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA CAGCCACATG CACTTCAAGA 
     4021 GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG 
     4081 AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA CCAGCACGCC TTCAAGACCC 
     4141 CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4201 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4261 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4321 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4381 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4441 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4501 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4561 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4621 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4681 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4741 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4801 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC TTGGCTGTGG 
     4861 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 
     4921 AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4981 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 
     5041 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT 
     5101 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 
     5161 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT 
     5221 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG 
     5281 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG 
     5341 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT 
     5401 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA 
     5461 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG 
     5521 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT 
     5581 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT 
     5641 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG 
     5701 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA 
     5761 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC 
     5821 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC 
     5881 GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT 
     5941 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC 
     6001 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA 
     6061 CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT 
     6121 CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA 
     6181 TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG 
     6241 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 
     6301 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA 
     6361 TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 
     6421 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 
     6481 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 
     6541 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG 
     6601 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC 
     6661 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     6721 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     6781 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     6841 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     6901 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     6961 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     7021 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     7081 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     7141 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     7201 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     7261 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     7321 CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 
     7381 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 
     7441 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 
     7501 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 
     7561 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 
     7621 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 
     7681 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 
     7741 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 
     7801 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 
     7861 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 
     7921 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 
     7981 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 
     8041 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 
     8101 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 
     8161 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 
     8221 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 
     8281 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 
     8341 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 
     8401 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8461 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8521 GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 
     8581 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 
     8641 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 
     8701 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 
     8761 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 
     8821 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 
     8881 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 
     8941 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//