LOCUS       RG215705                9215 bp    DNA     circular  SYN 
DEFINITION  PML (tGFP-tagged) - Human promyelocytic leukemia (PML), transcript variant 1
ACCESSION   NM_033238
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3694..4398 
                     /label="GFP"  
     misc_feature    4445..5030 
                     /label="PolyA signal"  
     misc_feature    7122..7767 
                     /label="ColE1"  
     misc_feature    5484..6278 
                     /label="Neo"  
     misc_feature    5115..5417 
                     /label="SV40 ori"  
     misc_feature    7782..8642 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3666..3673 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3666 
                     /label="PML"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCTGCA CCCGCCCGAT CTCCGAGGCC CCAGCAGGAC CCCGCCCGGC 
     1081 CCCAGGAGCC CACCATGCCT CCCCCCGAGA CCCCCTCTGA AGGCCGCCAG CCCAGCCCCA 
     1141 GCCCCAGCCC TACAGAGCGA GCCCCCGCTT CGGAGGAGGA GTTCCAGTTT CTGCGCTGCC 
     1201 AGCAATGCCA GGCGGAAGCC AAGTGCCCGA AGCTGCTGCC TTGTCTGCAC ACGCTGTGCT 
     1261 CAGGATGCCT GGAGGCGTCG GGCATGCAGT GCCCCATCTG CCAGGCGCCC TGGCCCCTAG 
     1321 GTGCAGACAC ACCCGCCCTG GATAACGTCT TTTTCGAGAG TCTGCAGCGG CGCCTGTCGG 
     1381 TGTACCGGCA GATTGTGGAT GCGCAGGCTG TGTGCACCCG CTGCAAAGAG TCGGCCGACT 
     1441 TCTGGTGCTT TGAGTGCGAG CAGCTCCTCT GCGCCAAGTG CTTCGAGGCA CACCAGTGGT 
     1501 TCCTCAAGCA CGAGGCCCGG CCCCTAGCAG AGCTGCGCAA CCAGTCGGTG CGTGAGTTCC 
     1561 TGGACGGCAC CCGCAAGACC AACAACATCT TCTGCTCCAA CCCCAACCAC CGCACCCCTA 
     1621 CGCTGACCAG CATCTACTGC CGAGGATGTT CCAAGCCGCT GTGCTGCTCG TGCGCGCTCC 
     1681 TTGACAGCAG CCACAGTGAG CTCAAGTGCG ACATCAGCGC AGAGATCCAG CAGCGACAGG 
     1741 AGGAGCTGGA CGCCATGACG CAGGCGCTGC AGGAGCAGGA TAGTGCCTTT GGCGCGGTTC 
     1801 ACGCGCAGAT GCACGCGGCC GTCGGCCAGC TGGGCCGCGC GCGTGCCGAG ACCGAGGAGC 
     1861 TGATCCGCGA GCGCGTGCGC CAGGTGGTAG CTCACGTGCG GGCTCAGGAG CGCGAGCTGC 
     1921 TGGAGGCTGT GGACGCGCGG TACCAGCGCG ACTACGAGGA GATGGCCAGT CGGCTGGGCC 
     1981 GCCTGGATGC TGTGCTGCAG CGCATCCGCA CGGGCAGCGC GCTGGTGCAG AGGATGAAGT 
     2041 GCTACGCCTC GGACCAGGAG GTGCTGGACA TGCACGGTTT CCTGCGCCAG GCGCTCTGCC 
     2101 GCCTGCGCCA GGAGGAGCCC CAGAGCCTGC AAGCTGCCGT GCGCACCGAT GGCTTCGACG 
     2161 AGTTCAAGGT GCGCCTGCAG GACCTCAGCT CTTGCATCAC CCAGGGGAAA GATGCAGCTG 
     2221 TATCCAAGAA AGCCAGCCCA GAGGCTGCCA GCACTCCCAG GGACCCTATT GACGTTGACC 
     2281 TGCCCGAGGA GGCAGAGAGA GTGAAGGCCC AGGTTCAGGC CCTGGGGCTG GCTGAAGCCC 
     2341 AGCCTATGGC TGTGGTACAG TCAGTGCCCG GGGCACACCC CGTGCCAGTG TACGCCTTCT 
     2401 CCATCAAAGG CCCTTCCTAT GGAGAGGATG TCTCCAATAC AACGACAGCC CAGAAGAGGA 
     2461 AGTGCAGCCA GACCCAGTGC CCCAGGAAGG TCATCAAGAT GGAGTCTGAG GAGGGGAAGG 
     2521 AGGCAAGGTT GGCTCGGAGC TCCCCGGAGC AGCCCAGGCC CAGCACCTCC AAGGCAGTCT 
     2581 CACCACCCCA CCTGGATGGA CCGCCTAGCC CCAGGAGCCC CGTCATAGGA AGTGAGGTCT 
     2641 TCCTGCCCAA CAGCAACCAC GTGGCCAGTG GCGCCGGGGA GGCAGAGGAA CGCGTTGTGG 
     2701 TGATCAGCAG CTCGGAAGAC TCAGATGCCG AAAACTCGTC CTCCCGAGAG CTGGATGACA 
     2761 GCAGCAGTGA GTCCAGTGAC CTCCAGCTGG AAGGCCCCAG CACCCTCAGG GTCCTGGACG 
     2821 AGAACCTTGC TGACCCCCAA GCAGAAGACA GACCTCTGGT TTTCTTTGAC CTCAAGATTG 
     2881 ACAATGAAAC CCAGAAGATT AGCCAGCTGG CTGCGGTGAA CCGGGAAAGC AAGTTCCGCG 
     2941 TGGTCATCCA GCCTGAAGCC YTCTTCAGCA TCTACTCCAA GGCCGTGTCC CTGGAGGTGG 
     3001 GGCTGCAGCA CTTCCTCAGC TTTCTGAGCT CCATGCGCCG CCCTATCTTG GCCTGCTACA 
     3061 AGCTGTGGGG GCCTGGCCTC CCAAACTTCT TCCGGGCCCT GGAGGACATT AACAGGCTGT 
     3121 GGGAATTCCA GGAGGCCATC TCGGGCTTCC TGGCTGCCCT GCCTCTCATC CGGGAGCGTG 
     3181 TGCCCGGGGC CAGCAGCTTC AAACTCAAGA ACCTGGCCCA GACCTACCTG GCGAGAAACA 
     3241 TGAGCGAGCG CAGCGCCATG GCTGCCGTGC TGGCCATGCG TGACCTGTGC CGCCTCCTCG 
     3301 AGGTCTCCCC GGGCCCCCAG CTGGCCCAGC ATGTCTACCC CTTCAGTAGC CTGCAGTGCT 
     3361 TTGCCTCCCT GCAGCCCCTG GTGCAGGCAG CTGTGCTGCC CCGGGCTGAG GCCCGCCTCC 
     3421 TGGCCCTACA CAACGTGAGC TTCATGGAGC TGCTGAGTGC ACACCGCCGT GACCGGCAGG 
     3481 GGGGCCTGAA GAAGTACAGC CGCTATCTAA GCCTGCAGAC CACCACGTTG CCCCCTGCCC 
     3541 AGCCTGCTTT CAACCTGCAG GCTCTGGGCA CCTACTTTGA AGGCCTGTTG GAGGGTCCGG 
     3601 CGCTGGCACG GGCAGAAGGA GTCTCCACCC CACTTGCTGG CCGTGGCTTG GCAGAGAGGG 
     3661 CCTCCCAGCA GAGCAGCGGA CCGACGCGTA CGCGGCCGCT CGAGATGGAG AGCGACGAGA 
     3721 GCGGCCTGCC CGCCATGGAG ATCGAGTGCC GCATCACCGG CACCCTGAAC GGCGTGGAGT 
     3781 TCGAGCTGGT GGGCGGCGGA GAGGGCACCC CCGAGCAGGG CCGCATGACC AACAAGATGA 
     3841 AGAGCACCAA AGGCGCCCTG ACCTTCAGCC CCTACCTGCT GAGCCACGTG ATGGGCTACG 
     3901 GCTTCTACCA CTTCGGCACC TACCCCAGCG GCTACGAGAA CCCCTTCCTG CACGCCATCA 
     3961 ACAACGGCGG CTACACCAAC ACCCGCATCG AGAAGTACGA GGACGGCGGC GTGCTGCACG 
     4021 TGAGCTTCAG CTACCGCTAC GAGGCCGGCC GCGTGATCGG CGACTTCAAG GTGATGGGCA 
     4081 CCGGCTTCCC CGAGGACAGC GTGATCTTCA CCGACAAGAT CATCCGCAGC AACGCCACCG 
     4141 TGGAGCACCT GCACCCCATG GGCGATAACG ATCTGGATGG CAGCTTCACC CGCACCTTCA 
     4201 GCCTGCGCGA CGGCGGCTAC TACAGCTCCG TGGTGGACAG CCACATGCAC TTCAAGAGCG 
     4261 CCATCCACCC CAGCATCCTG CAGAACGGGG GCCCCATGTT CGCCTTCCGC CGCGTGGAGG 
     4321 AGGATCACAG CAACACCGAG CTGGGCATCG TGGAGTACCA GCACGCCTTC AAGACCCCGG 
     4381 ATGCAGATGC CGGTGAAGAA AGAGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4441 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4501 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4561 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4621 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4681 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4741 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4801 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4861 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4921 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4981 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5041 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT 
     5101 GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5161 ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     5221 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC 
     5281 ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT 
     5341 TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA 
     5401 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC 
     5461 GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC 
     5521 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA 
     5581 ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT 
     5641 GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 
     5701 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA 
     5761 AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 
     5821 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG 
     5881 GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG 
     5941 GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 
     6001 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT 
     6061 GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 
     6121 TGTGGCCGGC TGGGTGTGGC CGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT 
     6181 GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT 
     6241 CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 
     6301 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA 
     6361 CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA 
     6421 TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG 
     6481 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 
     6541 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC 
     6601 CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 
     6661 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 
     6721 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 
     6781 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 
     6841 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 
     6901 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 
     6961 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 
     7021 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 
     7081 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 
     7141 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 
     7201 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 
     7261 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 
     7321 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 
     7381 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 
     7441 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC 
     7501 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 
     7561 CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 
     7621 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 
     7681 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 
     7741 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 
     7801 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 
     7861 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 
     7921 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 
     7981 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 
     8041 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 
     8101 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 
     8161 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 
     8221 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 
     8281 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 
     8341 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 
     8401 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 
     8461 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 
     8521 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 
     8581 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 
     8641 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 
     8701 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 
     8761 CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG 
     8821 TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 
     8881 CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG 
     8941 GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT 
     9001 AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT 
     9061 TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA 
     9121 TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 
     9181 ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//