LOCUS       RG215258                9236 bp    DNA     circular  SYN 
DEFINITION  DLG1 (tGFP-tagged) - Human discs, large homolog 1 (Drosophila) (DLG1), transcript variant 2
ACCESSION   NM_004087
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3717..4421 
                     /label="GFP"  
     misc_feature    4468..5053 
                     /label="PolyA signal"  
     misc_feature    7145..7790 
                     /label="ColE1"  
     misc_feature    5507..6301 
                     /label="Neo"  
     misc_feature    5138..5440 
                     /label="SV40 ori"  
     misc_feature    7805..8665 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3696..3702 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3696 
                     /label="DLG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTCCGG AAGCAAGATA CCCAGAGAGC ATTGCACCTT TTGGAGGAAT 
     1081 ATCGTTCAAA ACTAAGCCAA ACTGAAGACA GACAGCTCAG AAGTTCCATA GAACGGGTTA 
     1141 TTAACATATT TCAGAGCAAC CTCTTTCAGG CTTTAATAGA TATTCAAGAA TTTTATGAAG 
     1201 TGACCTTACT GGATAATCCA AAATGTATAG ATCGTTCAAA GCCGTCTGAA CCAATTCAAC 
     1261 CTGTGAATAC TTGGGAGATT TCCAGCCTTC CAAGCTCTAC TGTGACTTCA GAGACACTGC 
     1321 CAAGCAGCCT TAGCCCTAGT GTAGAGAAAT ACAGGTATCA GGATGAAGAT ACACCTCCTC 
     1381 AAGAGCATAT TTCCCCACAA ATCACAAATG AAGTGATAGG TCCAGAATTG GTTCATGTCT 
     1441 CAGAGAAGAA CTTATCAGAG ATTGAGAATG TCCATGGATT TGTTTCTCAT TCTCATATTT 
     1501 CACCAATAAA GGCAAATCCT CCCCCAGTAC TGGTCAACAC AGATAGCTTG GAAACACCAA 
     1561 CTTACGTTAA TGGCACAGAT GCAGATTATG AATATGAAGA AATCACACTT GAAAGGGGAA 
     1621 ATTCAGGGCT TGGTTTCAGC ATTGCAGGAG GTACGGACAA CCCACACATT GGAGATGACT 
     1681 CAAGTATTTT CATTACCAAA ATTATCACAG GGGGAGCAGC CGCCCAAGAT GGAAGATTGC 
     1741 GGGTCAATGA CTGTATATTA CGAGTAAATG AAGTAGATGT TCGTGATGTA ACACATAGCA 
     1801 AAGCAGTTGA AGCGTTGAAA GAAGCAGGGT CTATTGTACG CTTGTATGTA AAAAGAAGGA 
     1861 AACCAGTGTC AGAAAAAATA ATGGAAATAA AGCTCATTAA AGGTCCTAAA GGTCTTGGGT 
     1921 TTAGCATTGC TGGAGGTGTT GGAAATCAGC ATATTCCTGG GGATAATAGC ATCTATGTAA 
     1981 CCAAAATAAT TGAAGGAGGT GCAGCACATA AGGATGGCAA ACTTCAGATT GGAGATAAAC 
     2041 TTTTAGCAGT GAATAACGTA TGTTTAGAAG AAGTTACTCA TGAAGAAGCA GTAACTGCCT 
     2101 TAAAGAACAC ATCTGATTTT GTTTATTTGA AAGTGGCAAA ACCCACAAGT ATGTATATGA 
     2161 ATGATGGCTA TGCACCACCT GATATCACCA ACTCTTCTTC TCAGCCTGTT GATAACCATG 
     2221 TTAGCCCATC TTCCTTCTTG GGCCAGACAC CAGCATCTCC AGCCAGATAC TCCCCAGTTT 
     2281 CTAAAGCAGT ACTTGGAGAT GATGAAATTA CAAGGGAACC TAGAAAAGTT GTTCTTCATC 
     2341 GTGGCTCAAC GGGCCTTGGT TTCAACATTG TAGGAGGAGA AGATGGAGAA GGAATATTTA 
     2401 TTTCCTTTAT CTTAGCCGGA GGACCTGCTG ATCTAAGTGG AGAGCTCAGA AAAGGAGATC 
     2461 GTATTATATC GGTAAACAGT GTTGACCTCA GAGCTGCTAG TCATGAGCAG GCAGCAGCTG 
     2521 CATTGAAAAA TGCTGGCCAG GCTGTCACAA TTGTTGCACA ATATCGACCT GAAGAATACA 
     2581 GTCGTTTTGA AGCTAAAATA CATGATTTAC GGGAGCAGAT GATGAATAGT AGTATTAGTT 
     2641 CAGGGTCAGG TTCTCTTCGA ACTAGCCAGA AGCGATCCCT CTATGTCAGA GCCCTTTTTG 
     2701 ATTATGACAA GACTAAAGAC AGTGGGCTTC CCAGTCAGGG ACTGAACTTC AAATTTGGAG 
     2761 ATATCCTCCA TGTTATTAAT GCTTCTGATG ATGAATGGTG GCAAGCCAGG CAGGTTACAC 
     2821 CAGATGGTGA GAGCGATGAG GTCGGAGTGA TTCCCAGTAA ACGCAGAGTT GAGAAGAAAG 
     2881 AACGAGCCCG ATTAAAAACA GTGAAATTCA ATTCTAAAAC GAGAGATAAA GGGTCATTCA 
     2941 ATGACAAGCG TAAAAAGAAC CTCTTTTCCC GAAAATTCCC CTTCTACAAG AACAAGGACC 
     3001 AGAGTGAGCA GGAAACAAGT GATGCTGACC AGCATGTAAC TTCTAATGCC AGCGATAGTG 
     3061 AAAGTAGTTA CCGTGGTCAA GAAGAATACG TCTTATCTTA TGAACCAGTG AATCAACAAG 
     3121 AAGTTAATTA TACTCGACCA GTGATCATAT TGGGACCTAT GAAAGACAGG ATAAATGATG 
     3181 ACTTGATCTC AGAATTTCCT GACAAATTTG GATCCTGTGT TCCTCATACA ACTAGACCAA 
     3241 AACGAGATTA TGAGGTAGAT GGAAGAGATT ATCATTTTGT GACTTCAAGA GAGCAGATGG 
     3301 AAAAAGATAT CCAGGAACAT AAATTCATTG AAGCTGGCCA GTATAACAAT CATCTATATG 
     3361 GAACAAGTGT TCAGTCTGTA CGAGAAGTAG CAGAAAAGGG CAAACACTGT ATCCTTGATG 
     3421 TGTCTGGAAA TGCCATAAAG AGATTACAGA TTGCACAGCT TTACCCTATC TCCATTTTTA 
     3481 TTAAACCCAA ATCCATGGAA AATATCATGG AAATGAATAA GCGTCTAACA GAAGAACAAG 
     3541 CCAGAAAAAC ATTTGAGAGA GCCATGAAAC TGGAACAGGA GTTTACTGAA CATTTCACAG 
     3601 CTATTGTACA GGGGGATACG CTGGAAGACA TTTACAACCA AGTGAAACAG ATCATAGAAG 
     3661 AACAATCTGG TTCTTACATC TGGGTTCCGG CAAAAGAAAA GCTAACGCGT ACGCGGCCGC 
     3721 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3781 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3841 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3901 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3961 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     4021 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     4081 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     4141 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     4201 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     4261 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4321 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4381 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4441 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4501 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4561 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4621 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4681 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4741 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4801 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4861 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4921 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4981 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5041 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5101 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5161 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     5221 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     5281 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5341 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5401 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5461 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5521 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5581 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5641 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5701 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5761 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5821 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5881 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5941 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     6001 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     6061 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     6121 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     6181 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     6241 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6301 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6361 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6421 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6481 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6541 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6601 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6661 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6721 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6781 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6841 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6901 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6961 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     7021 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     7081 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     7141 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     7201 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     7261 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7321 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7381 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7441 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7501 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7561 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7621 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7681 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7741 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7801 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7861 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7921 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7981 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     8041 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     8101 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     8161 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     8221 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     8281 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8341 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8401 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8461 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8521 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8581 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8641 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8701 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8761 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8821 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8881 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8941 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     9001 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     9061 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     9121 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     9181 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//