LOCUS       RG214964                9041 bp    DNA     circular  SYN 
DEFINITION  RHBDF2 (tGFP-tagged) - Human rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant 2
ACCESSION   NM_001005498
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3522..4226 
                     /label="GFP"  
     misc_feature    4273..4858 
                     /label="PolyA signal"  
     misc_feature    6950..7595 
                     /label="ColE1"  
     misc_feature    5312..6106 
                     /label="Neo"  
     misc_feature    4943..5245 
                     /label="SV40 ori"  
     misc_feature    7610..8470 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3501..3507 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3501 
                     /label="RHBDF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCTGCT GACAAGAATG GCGGGAGCGT GTCCTCTGTG TCCAGCAGCC 
     1081 GCCTGCAGAG CCGGAAGCCA CCCAACCTCT CCATCACCAT CCCGCCACCC GAGAAAGAGA 
     1141 CCCAGGCCCC TGGCGAGCAG GACAGCATGC TGCCTGAGAG GAAGAACCCA GCCTACTTGA 
     1201 AGAGCGTCAG CCTCCAGGAG CCACGCAGCC GATGGCAGGA GAGTTCAGAG AAGCGCCCTG 
     1261 GCTTCCGCCG CCAGGCCTCA CTGTCCCAGA GCATCCGCAA GGGCGCAGCC CAGTGGTTTG 
     1321 GAGTCAGCGG CGACTGGGAG GGGCAGCGGC AGCAGTGGCA GCGCCGCAGC CTGCACCACT 
     1381 GCAGCATGCG CTACGGCCGC CTGAAGGCCT CGTGCCAGCG TGACCTGGAG CTCCCCAGCC 
     1441 AGGAGGCACC GTCCTTCCAG GGCACTGAGT CCCCAAAGCC CTGCAAGATG CCCAAGATTG 
     1501 TGGATCCGCT GGCCCGGGGC CGGGCCTTCC GCCACCCGGA GGAGATGGAC AGGCCCCACG 
     1561 CCCCGCACCC ACCGCTGACC CCCGGAGTCC TGTCCCTCAC CTCCTTCACC AGTGTCCGTT 
     1621 CTGGCTACTC CCACCTGCCA CGCCGCAAGA GAATGTCTGT GGCCCACATG AGCTTGCAAG 
     1681 CTGCCGCTGC CCTCCTCAAG GGGCGCTCGG TGCTGGATGC CACCGGACAG CGGTGCCGGG 
     1741 TGGTCAAGCG CAGCTTTGCC TTCCCGAGCT TCCTGGAGGA GGATGTGGTC GATGGGGCAG 
     1801 ACACGTTTGA CTCCTCCTTT TTTAGTAAGG AAGAAATGAG CTCCATGCCT GATGATGTCT 
     1861 TTGAGTCCCC CCCACTCTCT GCCAGCTACT TCCGAGGGAT CCCACACTCA GCCTCCCCTG 
     1921 TCTCCCCCGA TGGGGTGCAA ATCCCTCTGA AGGAGTATGG CCGAGCCCCA GTCCCCGGGC 
     1981 CCCGGCGCGG CAAGCGCATC GCCTCCAAGG TGAAGCACTT TGCCTTTGAT CGGAAGAAGC 
     2041 GGCACTACGG CCTCGGCGTG GTGGGCAACT GGCTGAACCG CAGCTACCGC CGCAGCATCA 
     2101 GCAGCACTGT GCAGCGGCAG CTGGAGAGCT TCGACAGCCA CCGGCCCTAC TTCACCTACT 
     2161 GGCTGACCTT CGTCCATGTC ATCATCACGC TGCTGGTGAT TTGCACGTAT GGCATCGCAC 
     2221 CCGTGGGCTT TGCCCAGCAC GTCACCACCC AGCTGGTGCT GCGGAACAAA GGTGTGTACG 
     2281 AGAGCGTGAA GTACATCCAG CAGGAGAACT TCTGGGTTGG CCCCAGCTCG ATTGACCTGA 
     2341 TCCACCTGGG GGCCAAGTTC TCACCCTGCA TCCGGAAGGA CGGGCAGATC GAGCAGCTGG 
     2401 TGCTGCGCGA GCGAGACCTG GAGCGGGACT CAGGCTGCTG TGTCCAGAAT GACCACTCCG 
     2461 GATGCATCCA GACCCAGCGG AAGGACTGCT CGGAGACTTT GGCCACTTTT GTCAAGTGGC 
     2521 AGGATGACAC TGGGCCCCCC ATGGACAAGT CTGATCTGGG CCAGAAGCGG ACTTCGGGGG 
     2581 CTGTCTGCCA CCAGGACCCC AGGACCTGCG AGGAGCCAGC CTCCAGCGGT GCCCACATCT 
     2641 GGCCCGATGA CATCACTAAG TGGCCGATCT GCACAGAGCA GGCCAGGAGC AACCACACAG 
     2701 GCTTCCTGCA CATGGACTGC GAGATCAAGG GCCGCCCCTG CTGCATCGGC ACCAAGGGCA 
     2761 GCTGTGAGAT CACCACCCGG GAATACTGTG AGTTCATGCA CGGCTATTTC CATGAGGAAG 
     2821 CAACACTCTG CTCCCAGGTG CACTGCTTGG ACAAGGTGTG TGGGCTGCTG CCCTTCCTCA 
     2881 ACCCTGAGGT CCCAGATCAG TTCTACAGGC TCTGGCTGTC TCTCTTCCTA CATGCTGGCG 
     2941 TGGTGCACTG CCTCGTGTCT GTGGTCTTTC AAATGACCAT CCTGAGGGAC CTGGAGAAGC 
     3001 TGGCCGGCTG GCACCGTATC GCCATCATCT TCATCCTCAG TGGCATCACA GGCAACCTCG 
     3061 CCAGTGCCAT CTTTCTCCCA TACCGGGCAG AGGTGGGCCC GGCCGGCTCA CAGTTCGGCC 
     3121 TCCTCGCCTG CCTCTTCGTG GAGCTCTTCC AGAGCTGGCC GCTGCTGGAG AGGCCCTGGA 
     3181 AGGCCTTCCT CAACCTCTCG GCCATCGTGC TCTTCCTGTT CATCTGTGGC CTCCTGCCCT 
     3241 GGATCGACAA CATCGCCCAC ATCTTCGGCT TCCTCAGTGG CCTGCTGCTG GCCTTCGCCT 
     3301 TCCTGCCCTA CATCACCTTC GGCACCAGCG ACAAGTACCG CAAGCGGGCA CTCATCCTGG 
     3361 TGTCACTGCT GGCCTTTGCC GGCCTCTTCG CCGCCCTCGT GCTGTGGCTG TACATCTACC 
     3421 CCATTAACTG GCCCTGGATC GAGCACCTCA CCTGCTTCCC CTTCACCAGC CGCTTCTGCG 
     3481 AGAAGTATGA GCTGGACCAG GTGCTGCACA CGCGTACGCG GCCGCTCGAG ATGGAGAGCG 
     3541 ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT CACCGGCACC CTGAACGGCG 
     3601 TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA GCAGGGCCGC ATGACCAACA 
     3661 AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA CCTGCTGAGC CACGTGATGG 
     3721 GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA CGAGAACCCC TTCCTGCACG 
     3781 CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA GTACGAGGAC GGCGGCGTGC 
     3841 TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT GATCGGCGAC TTCAAGGTGA 
     3901 TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA CAAGATCATC CGCAGCAACG 
     3961 CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT GGATGGCAGC TTCACCCGCA 
     4021 CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT GGACAGCCAC ATGCACTTCA 
     4081 AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC CATGTTCGCC TTCCGCCGCG 
     4141 TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA GTACCAGCAC GCCTTCAAGA 
     4201 CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4261 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4321 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4381 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4441 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4501 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4561 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4621 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4681 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4741 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4801 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4861 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCTTGGCTG 
     4921 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     4981 CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA 
     5041 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT 
     5101 CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA 
     5161 ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG 
     5221 TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC 
     5281 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA 
     5341 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 
     5401 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT 
     5461 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 
     5521 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 
     5581 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC 
     5641 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 
     5701 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT 
     5761 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 
     5821 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 
     5881 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC 
     5941 ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 
     6001 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC 
     6061 GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 
     6121 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 
     6181 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 
     6241 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 
     6301 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 
     6361 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 
     6421 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 
     6481 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 
     6541 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 
     6601 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 
     6661 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 
     6721 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 
     6781 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 
     6841 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 
     6901 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 
     6961 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 
     7021 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 
     7081 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 
     7141 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 
     7201 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 
     7261 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 
     7321 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 
     7381 AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     7441 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     7501 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     7561 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT 
     7621 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 
     7681 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA 
     7741 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC 
     7801 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT 
     7861 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG 
     7921 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 
     7981 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG 
     8041 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA 
     8101 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG 
     8161 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT 
     8221 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 
     8281 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA 
     8341 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG 
     8401 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC 
     8461 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT 
     8521 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 
     8581 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG 
     8641 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC 
     8701 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA 
     8761 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC 
     8821 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT 
     8881 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA 
     8941 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT 
     9001 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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