LOCUS       RG214854                9410 bp    DNA     circular  SYN 
DEFINITION  PCDHA4 (tGFP-tagged) - Human protocadherin alpha 4 (PCDHA4), transcript variant 1
ACCESSION   NM_018907
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3889..4593 
                     /label="GFP"  
     misc_feature    4640..5225 
                     /label="PolyA signal"  
     misc_feature    7317..7962 
                     /label="ColE1"  
     misc_feature    5679..6473 
                     /label="Neo"  
     misc_feature    5310..5612 
                     /label="SV40 ori"  
     misc_feature    7977..8837 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3861..3868 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3861 
                     /label="PCDHA4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTTTTCC TGGGGAAGCG GCCAGGAATC CCGGCGTCTG CTGCTCTTAC 
     1081 TTCTTCTCCT CGCAGCCTGG GAGGCAGGGA ACGGTCAGCT CCACTACTCG GTCTCCGAGG 
     1141 AGGCCAAACA CGGCACCTTC GTGGGCCGCA TCGCGCAGGA CCTGGGACTG GAGCTGGCGG 
     1201 AGCTGGTGCC GCGCCTGTTC CGGGTGGCGT CCAAGGGCCG CGGAGGCCTT CTGGAGGTAA 
     1261 ATCTGCAGAA TGGCATTTTG TTTGTGAATT CTCGGATCGA CCGGGAGGAG CTGTGCCGGC 
     1321 GGAGCGCGGA GTGCAGCATC CACCTGGAGG TGATCGTAGA CAGGCCGCTG CAGGTTTTCC 
     1381 ATGTGGACGT GGAGGTGAGG GACATTAACG ATAACCCGCC GGTGTTCCCA GCAACACAAA 
     1441 AGAACCTGTC CATCGCGGAA TCCAGGCCGC TTGACTCTCG GTTTCCACTA GAGGGCGCCT 
     1501 CGGATGCAGA TATCGGGGAG AACGCCCTGC TCACTTACAG ACTGAGCCCA AATGAATACT 
     1561 TTTCTCTGGA AAAACCACCT GATGACGAGC TGGTAAAAGG TCTTGGGCTT ATATTACGGA 
     1621 AATCTTTAGA CAGAGAAGAA GCTCCGGAGA TTTTTTTAGT GCTCACAGCC ACTGATGGAG 
     1681 GCAAACCCGA GTTGACTGGC ACCGTTCAGT TACTCATCAC AGTACTGGAT GCCAATGACA 
     1741 ATGCCCCAGC TTTTGACAGA ACCATTTATA AGGTGAGATT ACTAGAAAAT GTTCCTAATG 
     1801 GAACATTGGT AATTAAACTT AACGCCTCAG ATTTAGACGA AGGATTGAAT GGGGACATTG 
     1861 TTTATTCATT CTCAAATGAT ATTTCGCCAA ATGTGAAATC CAAGTTTCAC ATAGATCCAA 
     1921 TTACTGGACA AATTATTGTA AAGGGATATA TTGACTTTGA AGAAAGCAAA TCCTATGAAA 
     1981 TTATTGTAGA GGGCATTGAT AAGGGACAGC TCCCACTTTC TGGCCATTGT AGAGTTATTG 
     2041 TGGAAGTAGA AGACAACAAC GATAATGTCC CAGATTTGGA ATTCAAGTCT TTATCACTTC 
     2101 CAATTAGAGA GGACGCTCCA CTGGGTACAG TCATCGCCCT GATCAGCGTG TCCGACAAAG 
     2161 ACATGGGTGT CAATGGGCTG GTCACCTGCT CCTTGACGTC CCACGTCCCC TTCAAGCTGG 
     2221 TGTCCACCTT CAAGAATTAC TACTCGTTGG TGCTGGACAG TGCCCTGGAC CGCGAGAGCG 
     2281 TGTCAGCCTA TGAGCTGGTG GTGACCGCGC GAGACGGGGG CTCGCCTTCG CTGTGGGCCA 
     2341 CGGCCAGTGT TTCTGTGGAG GTGGCTGATG TGAACGACAA CGCTCCGGCG TTCGCGCAGC 
     2401 CCGAGTACAC AGTGTTCGTG AAGGAGAACA ACCCGCCGGG CTGCCACATC TTCACTGTGT 
     2461 CTGCGTGGGA CGCGGACGCG CAGGAGAACG CGCTGGTGTC CTACTCGCTG GTAGAGCGGC 
     2521 GGGTAGGGGA GCGCGCGCTG TCGAGCTACG TTTCGGTGCA TGCGGAGAGC GGCAAGGTGT 
     2581 ACGCGCTGCA GCCGCTGGAC CACGAGGAGC TAGAGCTGCT GCAGTTTCAG GTGACCGCTC 
     2641 GCGATGCCGG CGTGCCACCT CTGGGCAGCA ACGTGACGCT GCAGGTGTTC GTGCTGGACG 
     2701 AAAACGACAA CGCGCCAGCA CTGCTAGCGC CTCGGGCGGG TGGCACTGGT GGCGCAGTGA 
     2761 GCGAGCTGGT GCCATGGTCG GTGGGTGTGG GCCACGTGGT GGCAAAGGTG CGCGCGGTGG 
     2821 ATGCTGACTC GGGCTACAAC GCGTGGCTTT CGTACGAGCT GCAGCCGGGG ACTGGTGGCG 
     2881 CGCGCATCCC GTTCCGCGTG GGGCTGTACA CTGGCGAGAT CAGCACAACG CGTGCCCTGG 
     2941 ACGAAACGGA CGCTCCGCGC CACCGCCTAC TGGTACTGGT GAAGGACCAC GGCGAGCCCG 
     3001 CGCTGACGGC CACGGCCACT GTGCTGGTGT CACTTGTGGA GAGTGGACAG GCGCCAAAGG 
     3061 CCTCCTCACG GGCGTTGGTG GGCGCTGTGG GTCCCGATGC TGCGCTGGTG GATGTCAACG 
     3121 TATACCTGAT CATTGCCATC TGCGCGGTGT CCAGCCTTTT GGTGCTCACG CTGCTGCTGT 
     3181 ACACCGCGCT GCGGTGCTCT GCGCTGCCCA CCGAGGGCGC GTGCGCTCCG GGCAAGCCCA 
     3241 CGCTGGTGTG CTCCAGTGCG GTGGGGAGCT GGTCATACTC GCAGCAGAGG AGGCCGAGGG 
     3301 TGTGCTCTGG TGAGGGCCCA CCCAAGACCG ACCTCATGGC CTTCAGCCCC AGTTTACCTG 
     3361 ACTCTAGGGA CAGAGAAGAT CAGCTGCAGA CAACTGAGGA ATCCTTTGCA AAGCCACGAC 
     3421 AGCCCAACCC TGACTGGCGT TACTCTGCCT CCCTGAGAGC AGGCATGCAC AGCTCTGTGC 
     3481 ACCTAGAGGA GGCTGGCATT CTACGGGCTG GTCCAGGAGG GCCTGATCAG CAGTGGCCAA 
     3541 CAGTATCCAG TGCAACACCA GAACCAGAGG CAGGAGAAGT GTCCCCTCCA GTCGGTGCGG 
     3601 GTGTCAACAG CAACAGCTGG ACCTTTAAAT ACGGACCAGG CAACCCCAAA CAATCCGGTC 
     3661 CCGGTGAGTT GCCCGACAAA TTCATTATCC CAGGATCTCC TGCAATCATC TCCATCCGGC 
     3721 AGGAGCCTAC TAACAGCCAA ATTGACAAAA GTGACTTCAT AACCTTCGGC AAAAAGGAGG 
     3781 AGACCAAGAA AAAGAAGAAA AAGAAGAAGG GTAACAAGAC CCAGGAGAAA AAAGAGAAAG 
     3841 GGAACAGCAC GACTGACAAC AGTGACCAGA GCGGACCGAC GCGTACGCGG CCGCTCGAGA 
     3901 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     3961 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     4021 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     4081 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     4141 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     4201 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     4261 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     4321 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4381 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4441 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4501 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4561 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4621 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4681 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4741 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4801 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4861 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4921 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4981 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5041 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5101 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5161 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5221 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5281 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     5341 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5401 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5461 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5521 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5581 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5641 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5701 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5761 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5821 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     5881 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     5941 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     6001 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     6061 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     6121 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     6181 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     6241 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     6301 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6361 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6421 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6481 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6541 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6601 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6661 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6721 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6781 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6841 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6901 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     6961 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     7021 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     7081 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     7141 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     7201 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     7261 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     7321 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7381 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7441 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7501 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7561 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7621 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7681 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7741 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7801 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7861 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7921 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     7981 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     8041 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     8101 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     8161 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     8221 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     8281 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     8341 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8401 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8461 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8521 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8581 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8641 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8701 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8761 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8821 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     8881 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     8941 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     9001 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     9061 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     9121 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     9181 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     9241 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     9301 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9361 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//