LOCUS       RG214849                8783 bp    DNA     circular  SYN 
DEFINITION  RNASEL (tGFP-tagged) - Human ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) (RNASEL)
ACCESSION   NM_021133
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3264..3968 
                     /label="GFP"  
     misc_feature    4015..4600 
                     /label="PolyA signal"  
     misc_feature    6692..7337 
                     /label="ColE1"  
     misc_feature    5054..5848 
                     /label="Neo"  
     misc_feature    4685..4987 
                     /label="SV40 ori"  
     misc_feature    7352..8212 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3243..3249 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3243 
                     /label="RNASEL"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGCAGG GATCATAACA ACCCCCAGGA GGGACCCACG TCCTCCAGCG 
     1081 GTAGAAGGGC TGCAGTGGAA GACAATCACT TGCTGATTAA AGCTGTTCAA AACGAAGATG 
     1141 TTGACCTGGT CCAGCAATTG CTGGAAGGTG GAGCCAATGT TAATTTCCAG GAAGAGGAAG 
     1201 GGGGCTGGAC ACCTCTGCAT AACGCAGTAC AAATGAGCAG GGAGGACATT GTGGAACTTC 
     1261 TGCTTCGTCA TGGTGCTGAC CCTGTTCTGA GGAAGAAGAA TGGGGCCACG CCTTTTATCC 
     1321 TCGCAGCGAT TGCGGGGAGC GTGAAGCTGC TGAAACTTTT CCTTTCTAAA GGAGCAGATG 
     1381 TCAATGAGTG TGATTTTTAT GGCTTCACAG CCTTCATGGA AGCCGCTGTG TATGGTAAGG 
     1441 TCAAAGCCCT AAAATTCCTT TATAAGAGAG GAGCAAATGT GAATTTGAGG CGAAAGACAA 
     1501 AGGAGGATCA AGAGCGGCTG AGGAAAGGAG GGGCCACAGC TCTCATGGAC GCTGCTGAAA 
     1561 AAGGACACGT AGAGGTCTTG AAGATTCTCC TTGATGAGAT GGGGGCAGAT GTAAACGCCT 
     1621 GTGACAATAT GGGCAGAAAT GCCTTGATCC ATGCTCTCCT GAGCTCTGAC GATAGTGATG 
     1681 TGGAGGCTAT TACGCATCTG CTGCTGGACC ATGGGGCTGA TGTCAATGTG AGGGGAGAAA 
     1741 GAGGGAAGAC TCCCCTGATC CTGGCAGTGG AGAAGAAGCA CTTGGGTTTG GTGCAGAGGC 
     1801 TTCTGGAGCA AGAGCACATA GAGATTAATG ACACAGACAG TGATGGCAAA ACAGCACTGC 
     1861 TGCTTGCTGT TGAACTCAAA CTGAAGAAAA TCGCCGAGTT GCTGTGCAAA CGTGGAGCCA 
     1921 GTACAGATTG TGGGGATCTT GTTATGACAG CGAGGCGGAA TTATGACCAT TCCCTTGTGA 
     1981 AGGTTCTTCT CTCTCATGGA GCCAAAGAAG ATTTTCACCC TCCTGCTGAA GACTGGAAGC 
     2041 CTCAGAGCTC ACACTGGGGG GCAGCCCTGA AGGATCTCCA CAGAATATAC CGCCCTATGA 
     2101 TTGGCAAACT CAAGTTCTTT ATTGATGAAA AATACAAAAT TGCTGATACT TCAGAAGGAG 
     2161 GCATCTACCT GGGGTTCTAT GAGAAGCAAG AAGTAGCTGT GAAGACGTTC TGTGAGGGCA 
     2221 GCCCACGTGC ACAGCGGGAA GTCTCTTGTC TGCAAAGCAG CCGAGAGAAC AGTCACTTGG 
     2281 TGACATTCTA TGGGAGTGAG AGCCACAGGG GCCACTTGTT TGTGTGTGTC ACCCTCTGTG 
     2341 AGCAGACTCT GGAAGCGTGT TTGGATGTGC ACAGAGGGGA AGATGTGGAA AATGAGGAAG 
     2401 ATGAATTTGC CCGAAATGTC CTGTCATCTA TATTTAAGGC TGTTCAAGAA CTACACTTGT 
     2461 CCTGTGGATA CACCCACCAG GATCTGCAAC CACAAAACAT CTTAATAGAT TCTAAGAAAG 
     2521 CTGCTCACCT GGCAGATTTT GATAAGAGCA TCAAGTGGGC TGGAGATCCA CAGGAAGTCA 
     2581 AGAGAGATCT AGAGGACCTT GGACGGCTGG TCCTCTATGT GGTAAAGAAG GGAAGCATCT 
     2641 CATTTGAGGA TCTGAAAGCT CAAAGTAATG AAGAGGTGGT TCAACTTTCT CCAGATGAGG 
     2701 AAACTAAGGA CCTCATTCAT CGTCTCTTCC ATCCTGGGGA ACATGTGAGG GACTGTCTGA 
     2761 GTGACCTGCT GGGTCATCCC TTCTTTTGGA CTTGGGAGAG CCGCTATAGG ACGCTTCGGA 
     2821 ATGTGGGAAA TGAATCCGAC ATCAAAACAC GAAAATCTGA AAGTGAGATC CTCAGACTAC 
     2881 TGCAACCTGG GCCTTCTGAA CATTCCAAAA TTTTTGACAA GTGGACGACT AAGATTAATG 
     2941 AATGTGTTAT GAAAAAAATG AATAAGTTTT ATGAAAAAAG AGGCAATTTC TACCAGAACA 
     3001 CTGTGGGTGA TCTGCTAAAG TTCATCCGGA ATTTGGGAGA ACACATTGAT GAAGAAAAGC 
     3061 ATAAAAAGAT GAAATTAAAA ATTGGAGACC CTTCCCTGTA TTTTCAGAAG ACATTTCCAG 
     3121 ATCTGGTGAT CTATGTCTAC ACAAAACTAC AGAACACAGA ATATAGAAAG CATTTCCCCC 
     3181 AAACCCACAG TCCAAACAAG CCTCAGTGTG ATGGAGCTGG TGGGGCCAGT GGGTTGGCCA 
     3241 GCCCTGGGTG CACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3301 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3361 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3421 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     3481 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     3541 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     3601 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     3661 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     3721 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     3781 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     3841 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     3901 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     3961 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4021 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4081 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4141 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4201 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4261 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4321 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4381 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4441 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4501 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4561 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4621 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     4681 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     4741 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     4801 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     4861 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     4921 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     4981 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5041 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5101 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5161 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5221 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5281 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5341 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5401 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     5461 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     5521 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     5581 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     5641 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     5701 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     5761 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     5821 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     5881 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     5941 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6001 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6061 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6121 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6181 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6241 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6301 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6361 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6421 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     6481 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     6541 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     6601 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     6661 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     6721 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     6781 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     6841 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     6901 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     6961 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7021 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7081 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7141 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7201 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7261 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7321 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7381 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7441 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     7501 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     7561 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     7621 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     7681 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     7741 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     7801 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     7861 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     7921 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     7981 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8041 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8101 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8161 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8221 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8281 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8341 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8401 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     8461 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     8521 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     8581 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     8641 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     8701 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     8761 AACAAAAATT TAACGCGAAT TTT
//