LOCUS       RG214682                8822 bp    DNA     circular  SYN 
DEFINITION  NBN (tGFP-tagged) - Human nibrin (NBN)
ACCESSION   NM_002485
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3303..4007 
                     /label="GFP"  
     misc_feature    4054..4639 
                     /label="PolyA signal"  
     misc_feature    6731..7376 
                     /label="ColE1"  
     misc_feature    5093..5887 
                     /label="Neo"  
     misc_feature    4724..5026 
                     /label="SV40 ori"  
     misc_feature    7391..8251 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3282..3288 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3282 
                     /label="NBN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAAACTG CTGCCCGCCG CGGGCCCGGC AGGAGGAGAA CCATACAGAC 
     1081 TTTTGACTGG CGTTGAGTAC GTTGTTGGAA GGAAAAACTG TGCCATTCTG ATTGAAAATG 
     1141 ATCAGTCGAT CAGCCGAAAT CATGCTGTGT TAACTGCTAA CTTTTCTGTA ACCAACCTGA 
     1201 GTCAAACAGA TGAAATCCCT GTATTGACAT TAAAAGATAA TTCTAAGTAT GGTACCTTTG 
     1261 TTAATGAGGA AAAAATGCAG AATGGCTTTT CCCGAACTTT GAAGTCGGGG GATGGTATTA 
     1321 CTTTTGGAGT GTTTGGAAGT AAATTCAGAA TAGAGTATGA GCCTTTGGTT GCATGCTCTT 
     1381 CTTGTTTAGA TGTCTCTGGG AAAACTGCTT TAAATCAAGC TATATTGCAA CTTGGAGGAT 
     1441 TTACTGTAAA CAATTGGACA GAAGAATGCA CTCACCTTGT CATGGTATCA GTGAAAGTTA 
     1501 CCATTAAAAC AATATGTGCA CTCATTTGTG GACGTCCAAT TGTAAAGCCA GAATATTTTA 
     1561 CTGAATTCCT GAAAGCAGTT GAGTCCAAGA AGCAGCCTCC ACAAATTGAA AGTTTTTACC 
     1621 CACCTCTTGA TGAACCATCT ATTGGAAGTA AAAATGTTGA TCTGTCAGGA CGGCAGGAAA 
     1681 GAAAACAAAT CTTCAAAGGG AAAACATTTA TATTTTTGAA TGCCAAACAG CATAAGAAAT 
     1741 TGAGTTCCGC AGTTGTCTTT GGAGGTGGGG AAGCTAGGTT GATAACAGAA GAGAATGAAG 
     1801 AAGAACATAA TTTCTTTTTG GCTCCGGGAA CGTGTGTTGT TGATACAGGA ATAACAAACT 
     1861 CACAGACCTT AATTCCTGAC TGTCAGAAGA AATGGATTCA GTCAATAATG GATATGCTCC 
     1921 AAAGGCAAGG TCTTAGACCT ATTCCTGAAG CAGAAATTGG ATTGGCGGTG ATTTTCATGA 
     1981 CTACAAAGAA TTACTGTGAT CCTCAGGGCC ATCCCAGTAC AGGATTAAAG ACAACAACTC 
     2041 CAGGACCAAG CCTTTCACAA GGCGTGTCAG TTGATGAAAA ACTAATGCCA AGCGCCCCAG 
     2101 TGAACACTAC AACATACGTA GCTGACACAG AATCAGAGCA AGCAGATACA TGGGATTTGA 
     2161 GTGAAAGGCC AAAAGAAATC AAAGTCTCCA AAATGGAACA AAAATTCAGA ATGCTTTCAC 
     2221 AAGATGCACC CACTGTAAAG GAGTCCTGCA AAACAAGCTC TAATAATAAT AGTATGGTAT 
     2281 CAAATACTTT GGCTAAGATG AGAATCCCAA ACTATCAGCT TTCACCAACT AAATTGCCAA 
     2341 GTATAAATAA AAGTAAAGAT AGGGCTTCTC AGCAGCAGCA GACCAACTCC ATCAGAAACT 
     2401 ACTTTCAGCC GTCTACCAAA AAAAGGGAAA GGGATGAAGA AAATCAAGAA ATGTCTTCAT 
     2461 GCAAATCAGC AAGAATAGAA ACGTCTTGTT CTCTTTTAGA ACAAACACAA CCTGCTACAC 
     2521 CCTCATTGTG GAAAAATAAG GAGCAGCATC TATCTGAGAA TGAGCCTGTG GACACAAACT 
     2581 CAGACAATAA CTTATTTACA GATACAGATT TAAAATCTAT TGTGAAAAAT TCTGCCAGTA 
     2641 AATCTCATGC TGCAGAAAAG CTAAGATCAA ATAAAAAAAG GGAAATGGAT GATGTGGCCA 
     2701 TAGAAGATGA AGTATTGGAA CAGTTATTCA AGGACACAAA ACCAGAGTTA GAAATTGATG 
     2761 TGAAAGTTCA AAAACAGGAG GAAGATGTCA ATGTTAGAAA AAGGCCAAGG ATGGATATAG 
     2821 AAACAAATGA CACTTTCAGT GATGAAGCAG TACCAGAAAG TAGCAAAATA TCTCAAGAAA 
     2881 ATGAAATTGG GAAGAAACGT GAACTCAAGG AAGACTCACT ATGGTCAGCT AAAGAAATAT 
     2941 CTAACAATGA CAAACTTCAG GATGATAGTG AGATGCTTCC AAAAAAGCTG TTATTGACTG 
     3001 AATTTAGATC ACTGGTGATT AAAAACTCTA CTTCCAGAAA TCCATCTGGC ATAAATGATG 
     3061 ATTATGGTCA ACTAAAAAAT TTCAAGAAAT TCAAAAAGGT CACATATCCT GGAGCAGGAA 
     3121 AACTTCCACA CATCATTGGA GGATCAGATC TAATAGCTCA TCATGCTCGA AAGAATACAG 
     3181 AACTAGAAGA GTGGCTAAGG CAGGAAATGG AGGTACAAAA TCAACATGCA AAAGAAGAGT 
     3241 CTCTTGCTGA TGATCTTTTT AGATACAATC CTTATTTAAA AAGGAGAAGA ACGCGTACGC 
     3301 GGCCGCTCGA GATGGAGAGC GACGAGAGCG GCCTGCCCGC CATGGAGATC GAGTGCCGCA 
     3361 TCACCGGCAC CCTGAACGGC GTGGAGTTCG AGCTGGTGGG CGGCGGAGAG GGCACCCCCG 
     3421 AGCAGGGCCG CATGACCAAC AAGATGAAGA GCACCAAAGG CGCCCTGACC TTCAGCCCCT 
     3481 ACCTGCTGAG CCACGTGATG GGCTACGGCT TCTACCACTT CGGCACCTAC CCCAGCGGCT 
     3541 ACGAGAACCC CTTCCTGCAC GCCATCAACA ACGGCGGCTA CACCAACACC CGCATCGAGA 
     3601 AGTACGAGGA CGGCGGCGTG CTGCACGTGA GCTTCAGCTA CCGCTACGAG GCCGGCCGCG 
     3661 TGATCGGCGA CTTCAAGGTG ATGGGCACCG GCTTCCCCGA GGACAGCGTG ATCTTCACCG 
     3721 ACAAGATCAT CCGCAGCAAC GCCACCGTGG AGCACCTGCA CCCCATGGGC GATAACGATC 
     3781 TGGATGGCAG CTTCACCCGC ACCTTCAGCC TGCGCGACGG CGGCTACTAC AGCTCCGTGG 
     3841 TGGACAGCCA CATGCACTTC AAGAGCGCCA TCCACCCCAG CATCCTGCAG AACGGGGGCC 
     3901 CCATGTTCGC CTTCCGCCGC GTGGAGGAGG ATCACAGCAA CACCGAGCTG GGCATCGTGG 
     3961 AGTACCAGCA CGCCTTCAAG ACCCCGGATG CAGATGCCGG TGAAGAAAGA GTTTAAACGG 
     4021 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4081 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4141 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4201 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4261 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4321 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4381 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4441 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4501 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4561 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4621 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4681 GGTACGCGGC CAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 
     4741 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 
     4801 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     4861 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 
     4921 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC 
     4981 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 
     5041 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 
     5101 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 
     5161 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 
     5221 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 
     5281 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 
     5341 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 
     5401 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 
     5461 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 
     5521 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 
     5581 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 
     5641 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 
     5701 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCCGA CCGCTATCAG 
     5761 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 
     5821 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 
     5881 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA 
     5941 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA 
     6001 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT 
     6061 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA 
     6121 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA 
     6181 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT 
     6241 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
     6301 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
     6361 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA 
     6421 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA 
     6481 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG 
     6541 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 
     6601 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 
     6661 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 
     6721 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 
     6781 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 
     6841 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 
     6901 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 
     6961 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 
     7021 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 
     7081 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 
     7141 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC 
     7201 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 
     7261 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 
     7321 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 
     7381 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 
     7441 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 
     7501 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 
     7561 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 
     7621 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 
     7681 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 
     7741 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 
     7801 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 
     7861 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 
     7921 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 
     7981 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 
     8041 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 
     8101 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 
     8161 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 
     8221 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 
     8281 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     8341 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     8401 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     8461 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     8521 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     8581 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     8641 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     8701 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     8761 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     8821 TT
//