LOCUS       RG214524                9248 bp    DNA     circular  SYN 
DEFINITION  PCDH10 (tGFP-tagged) - Human protocadherin 10 (PCDH10), transcript variant 2
ACCESSION   NM_020815
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3729..4433 
                     /label="GFP"  
     misc_feature    4480..5065 
                     /label="PolyA signal"  
     misc_feature    7157..7802 
                     /label="ColE1"  
     misc_feature    5519..6313 
                     /label="Neo"  
     misc_feature    5150..5452 
                     /label="SV40 ori"  
     misc_feature    7817..8677 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3708..3714 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3708 
                     /label="PCDH10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTGTGCTA TTATTGTTTG CCTTGCTCTG GATGGTGGAA GGAGTCTTTT 
     1081 CCCAGCTTCA CTACACGGTA CAGGAGGAGC AGGAACATGG CACTTTCGTG GGGAATATCG 
     1141 CTGAAGATCT GGGTCTGGAC ATTACAAAAC TTTCGGCTCG CGGGTTTCAG ACGGTGCCCA 
     1201 ACTCAAGGAC CCCTTACTTA GACCTCAACC TGGAGACAGG GGTGCTGTAC GTGAACGAGA 
     1261 AAATAGACCG CGAACAAATC TGCAAACAGA GCCCCTCCTG TGTCCTGCAC CTGGAGGTCT 
     1321 TTCTGGAGAA CCCCCTGGAG CTGTTCCAGG TGGAGATCGA GGTGCTGGAC ATTAATGACA 
     1381 ACCCCCCCTC TTTCCCGGAG CCAGACCTGA CGGTGGAAAT CTCTGAGAGC GCCACGCCAG 
     1441 GCACTCGCTT CCCCTTGGAG AGCGCATTCG ACCCAGACGT GGGCACCAAC TCCTTGCGCG 
     1501 ACTACGAGAT CACCCCCAAC AGCTACTTCT CCCTGGACGT GCAGACCCAG GGGGATGGCA 
     1561 ACCGATTCGC TGAGCTGGTG CTGGAGAAGC CACTGGACCG AGAGCAGCAA GCGGTGCACC 
     1621 GCTACGTGCT GACCGCGGTG GACGGAGGAG GTGGGGGAGG AGTAGGAGAA GGAGGGGGAG 
     1681 GTGGCGGGGG AGCAGGCCTG CCCCCCCAGC AGCAGCGCAC CGGCACGGCC CTACTCACCA 
     1741 TCCGAGTGCT GGACTCCAAT GACAATGTGC CCGCTTTCGA CCAACCCGTC TACACTGTGT 
     1801 CCCTACCAGA GAACTCTCCC CCAGGCACTC TCGTGATCCA GCTCAACGCC ACCGACCCGG 
     1861 ACGAGGGCCA GAACGGTGAG GTCGTGTACT CCTTCAGCAG CCACATTTCG CCCCGGGCGC 
     1921 GGGAGCTTTT CGGACTCTCG CCGCGCACTG GCAGACTGGA GGTAAGCGGC GAGTTGGACT 
     1981 ATGAAGAGAG CCCAGTGTAC CAAGTGTACG TGCAAGCCAA GGACCTGGGC CCCAACGCCG 
     2041 TGCCTGCGCA CTGCAAGGTG CTAGTGCGAG TACTGGATGC TAATGACAAC GCGCCAGAGA 
     2101 TCAGCTTCAG CACCGTGAAG GAAGCGGTGA GTGAGGGCGC GGCGCCCGGC ACTGTGGTGG 
     2161 CCCTTTTCAG CGTGACTGAC CGCGACTCAG AGGAGAATGG GCAGGTGCAG TGCGAGCTAC 
     2221 TGGGAGACGT GCCTTTCCGC CTCAAGTCTT CCTTTAAGAA TTACTACACC ATCGTTACCG 
     2281 AAGCCCCCCT GGACCGAGAG GCGGGGGACT CCTACACCCT GACTGTAGTG GCTCGGGACC 
     2341 GGGGCGAGCC TGCGCTCTCC ACCAGTAAGT CGATCCAGGT ACAAGTGTCG GATGTGAACG 
     2401 ACAACGCGCC GCGTTTCAGC CAGCCGGTCT ACGACGTGTA TGTGACTGAA AACAACGTGC 
     2461 CTGGCGCCTA CATCTACGCG GTGAGCGCCA CCGACCGGGA TGAGGGCGCC AACGCCCAGC 
     2521 TTGCCTACTC TATCCTCGAG TGCCAGATCC AGGGCATGAG CGTCTTCACC TACGTTTCTA 
     2581 TCAACTCTGA GAACGGCTAC TTGTACGCCC TGCGCTCCTT CGACTATGAG CAGCTGAAGG 
     2641 ACTTCAGTTT TCAGGTGGAA GCCCGGGACG CTGGCAGCCC CCAGGCGCTG GCTGGTAACG 
     2701 CCACTGTCAA CATCCTCATA GTGGATCAAA ATGACAACGC CCCTGCCATC GTGGCGCCTC 
     2761 TACCAGGGCG CAACGGGACT CCAGCGCGTG AGGTGCTGCC CCGCTCGGCG GAGCCGGGTT 
     2821 ACCTGCTCAC CCGCGTGGCC GCCGTGGACG CGGACGACGG CGAGAACGCC CGGCTCACTT 
     2881 ACAGCATCGT GCGTGGCAAC GAAATGAACC TCTTTCGCAT GGACTGGCGC ACCGGGGAGC 
     2941 TGCGCACAGC ACGCCGAGTC CCGGCCAAGC GCGACCCCCA GCGGCCTTAT GAGCTGGTGA 
     3001 TCGAGGTGCG CGACCATGGG CAGCCGCCCC TTTCCTCCAC CGCCACCCTG GTGGTTCAGC 
     3061 TGGTGGATGG CGCCGTGGAG CCCCAGGGCG GGGGCGGGAG CGGAGGCGGA GGGTCAGGAG 
     3121 AGCACCAGCG CCCCAGTCGC TCTGGCGGCG GGGAAACCTC GCTAGACCTC ACCCTCATCC 
     3181 TCATCATCGC GTTGGGCTCG GTGTCCTTCA TCTTCCTGCT GGCCATGATC GTGCTGGCCG 
     3241 TGCGTTGCCA AAAAGAGAAG AAGCTCAACA TCTATACTTG TCTGGCCAGC GATTGCTGCC 
     3301 TCTGCTGCTG CTGCTGCGGT GGCGGAGGTT CGACCTGCTG TGGCCGCCAA GCCCGGGCGC 
     3361 GCAAGAAGAA ACTCAGCAAG TCAGACATCA TGCTGGTGCA GAGCTCCAAT GTACCCAGTA 
     3421 ACCCGGCCCA GGTGCCGATA GAGGAGTCCG GGGGCTTTGG CTCCCACCAC CACAACCAGA 
     3481 ATTACTGCTA TCAGGTATGC CTGACCCCTG AGTCCGCCAA GACCGACCTG ATGTTTCTTA 
     3541 AGCCCTGCAG CCCTTCGCGG AGTACGGACA CTGAGCACAA CCCCTGCGGG GCCATCGTCA 
     3601 CCGGTTACAC CGACCAGCAG CCTGATATCA TCTCCAACGG AAGCATTTTG TCCAACGAGG 
     3661 TAAGGCTGAA GCGAAAGGAC CACCATCTCT CATCTCCTCC ATCAGAAAGC CTCCTCACGC 
     3721 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3781 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3841 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3901 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3961 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4021 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4081 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4141 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4201 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4261 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4321 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4381 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4441 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4501 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4561 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4621 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4681 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4741 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4801 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4861 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4921 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4981 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5041 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5101 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5161 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5221 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5281 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5341 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5401 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5461 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5521 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5581 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5641 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5701 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5761 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5821 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5881 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5941 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6001 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6061 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6121 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6181 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6241 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6301 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6361 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6421 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6481 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6541 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6601 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6661 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6721 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6781 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6841 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6901 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6961 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7021 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7081 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7141 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7201 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7261 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7321 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7381 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7441 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7501 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7561 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7621 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7681 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7741 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7801 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7861 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7921 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7981 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8041 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8101 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8161 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8221 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8281 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8341 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8401 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8461 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8521 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8581 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8641 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8701 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8761 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8821 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8881 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8941 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9001 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9061 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9121 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9181 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9241 CGAATTTT
//