LOCUS       RG214488                9428 bp    DNA     circular  SYN 
DEFINITION  GRIK4 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 4 (GRIK4)
ACCESSION   NM_014619
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3909..4613 
                     /label="GFP"  
     misc_feature    4660..5245 
                     /label="PolyA signal"  
     misc_feature    7337..7982 
                     /label="ColE1"  
     misc_feature    5699..6493 
                     /label="Neo"  
     misc_feature    5330..5632 
                     /label="SV40 ori"  
     misc_feature    7997..8857 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3888..3894 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3888 
                     /label="GRIK4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCGTC TCGGCGCCTT TGGTGCTGCT TCCTGCGTGG CTCGTGATGG 
     1081 TCGCCTGCAG CCCGCACTCC TTGAGGATCG CTGCTATCTT GGACGACCCC ATGGAGTGCA 
     1141 GCAGAGGGGA GCGGCTCTCC ATCACCCTGG CCAAGAACCG CATCAACCGC GCTCCTGAGA 
     1201 GGCTGGGCAA GGCCAAGGTC GAAGTGGACA TCTTTGAGCT TCTCAGAGAC AGCGAGTACG 
     1261 AGACTGCAGA AACCATGTGT CAGATCCTCC CCAAGGGGGT GGTCGCTGTC CTCGGACCAT 
     1321 CGTCCAGCCC AGCCTCCAGC TCCATCATCA GCAACATCTG TGGAGAGAAG GAGGTCCCTC 
     1381 ACTTCAAAGT GGCCCCAGAG GAGTTCGTCA AGTTCCAGTT CCAGAGATTC ACAACCCTGA 
     1441 ACCTCCACCC CAGCAACACT GACATCAGCG TGGCTGTAGC TGGGATCCTG AACTTCTTCA 
     1501 ACTGCACCAC CGCCTGCCTC ATCTGTGCCA AAGCAGAATG CCTTTTAAAC CTAGAGAAGC 
     1561 TGCTCCGGCA ATTCCTTATC TCCAAGGACA CGCTGTCCGT CCGCATGCTG GATGACACCC 
     1621 GGGACCCCAC CCCGCTCCTC AAGGAGATCC GGGACGACAA GACCGCCACC ATCATCATCC 
     1681 ACGCCAACGC CTCCATGTCC CACACCATCC TCCTGAAGGC AGCCGAACTT GGGATGGTGT 
     1741 CAGCCTATTA CACATACATC TTCACTAATC TGGAGTTCTC ACTCCAGAGA ATGGACAGCC 
     1801 TTGTGGATGA TCGTGTCAAC ATCCTGGGAT TTTCCATTTT CAACCAATCC CATGCTTTCT 
     1861 TCCAAGAGTT TGCCCAGAGC CTCAACCAGT CCTGGCAGGA GAACTGTGAC CATGTGCCCT 
     1921 TCACTGGGCC TGCGCTCTCC TCGGCCCTGC TGTTTGATGC TGTCTATGCT GTGGTGACTG 
     1981 CGGTGCAGGA ACTGAACCGG AGCCAAGAGA TCGGCGTGAA GCCCTTGTCC TGCGGCTCGG 
     2041 CCCAGATCTG GCAGCACGGC ACCAGCCTCA TGAACTACCT GCGCATGGTA GAATTGGAAG 
     2101 GTCTTACCGG CCACATTGAA TTCAACAGCA AAGGCCAGAG GTCCAACTAC GCTTTGAAAA 
     2161 TCTTACAGTT CACAAGGAAT GGTTTTCGGC AGATCGGCCA GTGGCACGTG GCAGAGGGCC 
     2221 TCAGCATGGA CAGCCACCTC TATGCCTCCA ACATCTCGGA CACTCTCTTC AACACCACCC 
     2281 TGGTCGTCAC CACCATCCTG GAAAACCCAT ATTTAATGCT GAAGGGGAAC CACCAGGAGA 
     2341 TGGAAGGCAA TGACCGCTAC GAGGGCTTCT GTGTGGACAT GCTCAAGGAG CTGGCAGAGA 
     2401 TCCTCCGATT CAACTACAAG ATCCGCCTGG TTGGGGATGG CGTGTACGGC GTTCCCGAGG 
     2461 CCAACGGCAC CTGGACGGGA ATGGTCGGGG AGCTGATCGC TAGGAAAGCA GATCTGGCTG 
     2521 TGGCAGGCCT CACCATTACA GCTGAACGGG AGAAGGTGAT TGATTTCTCT AAGCCATTCA 
     2581 TGACTCTGGG AATTAGCATT CTTTACCGCG TTCATATGGG ACGCAAACCC GGCTATTTCT 
     2641 CCTTCCTGGA CCCATTTTCT CCGGGCGTCT GGCTCTTCAT GCTTCTAGCC TATCTGGCCG 
     2701 TCAGCTGTGT CCTCTTCCTG GTGGCTCGGT TGACGCCCTA CGAGTGGTAC AGCCCACACC 
     2761 CATGTGCCCA GGGCCGGTGC AACCTCCTGG TGAACCAGTA CTCCCTGGGC AACAGCCTCT 
     2821 GGTTTCCGGT CGGGGGGTTC ATGCAGCAAG GCTCCACCAT CGCCCCTCGC GCCTTATCCA 
     2881 CCCGCTGTGT CAGTGGCGTC TGGTGGGCAT TCACGCTGAT CATCATCTCA TCCTACACGG 
     2941 CCAACCTGGC AGCCTTCCTG ACCGTGCAGC GCATGGATGT GCCCATTGAG TCAGTGGATG 
     3001 ACCTGGCTGA CCAGACCGCC ATTGAATATG GCACAATTCA CGGAGGCTCC AGCATGACCT 
     3061 TCTTCCAAAA TTCCCGCTAC CAGACCTACC AACGCATGTG GAATTACATG TATTCCAAGC 
     3121 AGCCCAGCGT GTTCGTGAAG AGCACAGAGG AGGGAATCGC CAGGGTGTTG AATTCCAACT 
     3181 ACGCCTTCCT CCTGGAATCC ACCATGAACG AGTACTATCG GCAGCGAAAC TGCAACCTCA 
     3241 CTCAGATTGG GGGCCTGCTG GACACCAAGG GCTATGGGAT TGGCATGCCA GTCGGCTCGG 
     3301 TTTTCCGGGA CGAGTTTGAT CTGGCCATTC TCCAGCTGCA GGAGAACAAC CGCCTGGAGA 
     3361 TCCTGAAGCG CAAATGGTGG GAAGGAGGGA AGTGCCCCAA GGAGGAAGAT CACAGAGCTA 
     3421 AAGGCCTGGG AATGGAGAAT ATTGGTGGAA TCTTTGTGGT TCTTATTTGT GGCTTAATCG 
     3481 TGGCCATTTT TATGGCTATG TTGGAGTTTT TATGGACTCT CAGACACTCA GAAGCAACTG 
     3541 AGGTGTCCGT CTGCCAGGAG ATGGTGACCG AGCTGCGCAG CATTATCCTG TGTCAGGACA 
     3601 GTATCCACCC CCGCCGGCGG CGCGCCGCAG TCCCGCCGCC CCGGCCCCCC ATCCCCGAGG 
     3661 AGCGCCGACC GCGGGGCACG GCGACGCTCA GCAACGGGAA GCTGTGCGGG GCAGGGGAGC 
     3721 CCGACCAGCT CGCGCAGAGA CTGGCGCAGG AGGCCGCCCT GGTGGCCCGC GGCTGCACGC 
     3781 ACATCCGCGT CTGCCCCGAG TGCCGCCGCT TCCAGGGCCT GCGGGCACGG CCGTCGCCCG 
     3841 CCCGCAGCGA GGAGAGCCTG GAGTGGGAGA AAACCACCAA CAGCAGCGAG CCCGAGACGC 
     3901 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3961 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     4021 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     4081 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     4141 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     4201 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     4261 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     4321 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     4381 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     4441 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     4501 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     4561 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     5341 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     5401 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     5461 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     5521 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     5581 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5641 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5701 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5761 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5821 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5881 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5941 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     6001 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     6061 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     6121 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     6181 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     6241 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     6301 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     6361 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     6421 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     6481 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     6541 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6601 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6661 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6721 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6781 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6841 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6901 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6961 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     7021 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     7081 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     7141 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     7201 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     7261 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     7321 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     7381 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     7441 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     7501 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     7561 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7621 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7681 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7741 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     8041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     8101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     8161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     8221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     8281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     8341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     8401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     8461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     8521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     8581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     9001 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     9061 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     9121 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     9181 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     9241 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     9301 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     9361 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9421 CGAATTTT
//