LOCUS       RG214305                9050 bp    DNA     circular  SYN 
DEFINITION  PDE8B (tGFP-tagged) - Human phosphodiesterase 8B (PDE8B), transcript variant 5
ACCESSION   NM_001029852
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3531..4235 
                     /label="GFP"  
     misc_feature    4282..4867 
                     /label="PolyA signal"  
     misc_feature    6959..7604 
                     /label="ColE1"  
     misc_feature    5321..6115 
                     /label="Neo"  
     misc_feature    4952..5254 
                     /label="SV40 ori"  
     misc_feature    7619..8479 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3510..3516 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3510 
                     /label="PDE8B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTGCGCC CCCAGCATCC ATGTCTCGCA GAGCGGCGTG ATCTACTGCC 
     1081 GGGACTCGGA CGAGTCCAGC TCGCCCCGCC AGACCACCAG CGTGTCGCAG GGCCCGGCGG 
     1141 CACCCCTGCC CGGCCTCTTC GTCCAGACCG ACGCCGCCGA CGCCATCCCC CCGAGCCGCG 
     1201 CGTCGGGACC CCCCAGCGTA GCCCGCGTCC GCAGGGCCCG CACCGAGCTG GGCAGCGGTA 
     1261 GCAGCGCGGG TTCCGCAGCC CCCGCCGCGA CCACCAGCAG GGGCCGGAGG CGCCACTGCT 
     1321 GCAGCAGCGC CGAGGCCGAG ACTCAGACCT GCTACACCAG CGTGAAGCAG GTGTCTTCTG 
     1381 CGGAGGTGCG CATCGGGCCC ATGAGACTGA CGCAGGACCC TATTCAGGTT TTGCTGATCT 
     1441 TTGCAAAGGA AGATAGTCAG AGCGATGGCT TCTGGTGGGC CTGCGACAGA GCTGGTTATA 
     1501 GATGCAATAT TGCTCGGACT CCAGAGTCAG CCCTTGAATG CTTTCTTGAT AAGCATCATG 
     1561 AAATTATTGT AATTGATCAT AGACAAACTC AGAACTTCGA TGCAGAAGCA GTGTGCAGGT 
     1621 CGATCCGGGC CACAAATCCC TCCGAGCACA CGGTGATCCT CGCAGTGGTT TCGCGAGTAT 
     1681 CGGATGACCA TGAAGAGGCG TCAGTCCTTC CTCTTCTCCA CGCAGGCTTC AACAGGAGAT 
     1741 TTATGGAGAA TAGCAGCATA ATTGCTTGCT ATAATGAACT GATTCAAATA GAACATGGGG 
     1801 AAGTTCGCTC CCAGTTCAAA TTACGGGCCT GTAATTCAGT GTTTACAGCA TTAGATCACT 
     1861 GTCATGAAGC CATAGAAATA ACAAGCGATG ACCACGTGAT TCAGTATGTC AACCCAGCCT 
     1921 TCGAAAGGAT GATGGGCTAC CACAAAGGTG AGCTCCTGGG AAAAGAACTC GCTGATCTGC 
     1981 CCAAAAGCGA TAAGAACCGG GCAGACCTTC TCGACACCAT CAATACATGC ATCAAGAAGG 
     2041 GAAAGGAGTG GCAGGGGGTT TACTATGCCA GACGGAAATC CGGGGACAGC ATCCAACAGC 
     2101 ACGTGAAGAT CACCCCAGTG ATTGGCCAAG GAGGGAAAAT TAGGCATTTT GTCTCGCTCA 
     2161 AGAAACTGTG TTGTACCACT GACAATAATA AGCAGATTCA CAAGATTCAT CGTGATTCAG 
     2221 GAGACAATTC TCAGACAGAG CCTCATTCAT TCAGATATAA GAACAGGAGG AAAGAGTCCA 
     2281 TTGACGTGAA ATCGATATCA TCTCGAGGCA GTGATGCACC AAGCCTGCAG AATCGTCGCT 
     2341 ATCCGTCCAT GGCGAGGATC CACTCCATGA CCATCGAGGC TCCCATCACA AAGGACGGCT 
     2401 TGAGAAGACT GTCAGGAAAC GAGTATGTGT TTACTAAGAA TGTGCACCAG AGTCACAGTC 
     2461 ACCTTGCAAT GCCAATAACC ATCAATGATG TTCCCCCTTG TATCTCTCAA TTACTTGATA 
     2521 ATGAGGAGAG TTGGGACTTC AACATCTTTG AATTGGAAGC CATTACGCAT AAAAGGCCAT 
     2581 TGGTTTATCT GGGCTTAAAG GTCTTCTCTC GGTTTGGAGT ATGTGAATTT TTAAACTGTT 
     2641 CTGAAACCAC TCTTCGGGCC TGGTTCCAAG TGATCGAAGC CAACTACCAC TCTTCCAATG 
     2701 CCTACCACAA CTCCACCCAT GCTGCCGACG TCCTGCACGC CACCGCTTTC TTTCTTGGAA 
     2761 AGGAAAGAGT AAAGGGAAGC CTCGATCAGT TGGATGAGGT GGCAGCCCTC ATTGCTGCCA 
     2821 CAGTCCATGA CGTGGATCAC CCGGGAAGGA CCAACTCTTT CCTCTGCAAT GCAGGCAGTG 
     2881 AGCTTGCTGT GCTCTACAAT GACACTGCTG TTCTGGAGAG TCACCACACC GCCCTGGCCT 
     2941 TCCAGCTCAC GGTCAAGGAC ACCAAATGCA ACATTTTCAA GAATATTGAC AGGAACCATT 
     3001 ATCGAACGCT GCGCCAGGCT ATTATTGACA TGGTTTTGGC AACAGAGATG ACAAAACACT 
     3061 TTGAACATGT GAATAAGTTT GTGAACAGCA TCAACAAGCC AATGGCAGCT GAGATTGAAG 
     3121 GCAGCGACTG TGAATGCAAC CCTGCTGGGA AGAACTTCCC TGAAAACCAA ATCCTGATCA 
     3181 AACGCATGAT GATTAAGTGT GCTGACGTGG CCAACCCATG CCGCCCCTTG GACCTGTGCA 
     3241 TTGAATGGGC TGGGAGGATC TCTGAGGAGT ATTTTGCACA GACTGATGAA GAGAAGAGAC 
     3301 AGGGACTACC TGTGGTGATG CCAGTGTTTG ACCGGAATAC CTGTAGCATC CCCAAGTCTC 
     3361 AGATCTCTTT CATTGACTAC TTCATAACAG ACATGTTTGA TGCTTGGGAT GCCTTTGCAC 
     3421 ATCTGCCAGC CCTGATGCAA CATTTGGCTG ACAACTACAA ACACTGGAAG ACACTAGATG 
     3481 ACCTAAAGTG CAAAAGTTTG AGGCTTCCAT CTGACAGCAC GCGTACGCGG CCGCTCGAGA 
     3541 TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA TGGAGATCGA GTGCCGCATC ACCGGCACCC 
     3601 TGAACGGCGT GGAGTTCGAG CTGGTGGGCG GCGGAGAGGG CACCCCCGAG CAGGGCCGCA 
     3661 TGACCAACAA GATGAAGAGC ACCAAAGGCG CCCTGACCTT CAGCCCCTAC CTGCTGAGCC 
     3721 ACGTGATGGG CTACGGCTTC TACCACTTCG GCACCTACCC CAGCGGCTAC GAGAACCCCT 
     3781 TCCTGCACGC CATCAACAAC GGCGGCTACA CCAACACCCG CATCGAGAAG TACGAGGACG 
     3841 GCGGCGTGCT GCACGTGAGC TTCAGCTACC GCTACGAGGC CGGCCGCGTG ATCGGCGACT 
     3901 TCAAGGTGAT GGGCACCGGC TTCCCCGAGG ACAGCGTGAT CTTCACCGAC AAGATCATCC 
     3961 GCAGCAACGC CACCGTGGAG CACCTGCACC CCATGGGCGA TAACGATCTG GATGGCAGCT 
     4021 TCACCCGCAC CTTCAGCCTG CGCGACGGCG GCTACTACAG CTCCGTGGTG GACAGCCACA 
     4081 TGCACTTCAA GAGCGCCATC CACCCCAGCA TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT 
     4141 TCCGCCGCGT GGAGGAGGAT CACAGCAACA CCGAGCTGGG CATCGTGGAG TACCAGCACG 
     4201 CCTTCAAGAC CCCGGATGCA GATGCCGGTG AAGAAAGAGT TTAAACGGCC GGCCGCGGTC 
     4261 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4321 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4381 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4441 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4501 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4561 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4621 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4681 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4741 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4801 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4861 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4921 GCTTGGCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 
     4981 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC 
     5041 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG 
     5101 CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT 
     5161 GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC 
     5221 CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT 
     5281 TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA 
     5341 CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC 
     5401 TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 
     5461 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG 
     5521 GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 
     5581 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG 
     5641 TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG 
     5701 CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 
     5761 GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG 
     5821 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT 
     5881 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT 
     5941 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCCGACC GCTATCAGGA CATAGCGTTG 
     6001 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 
     6061 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC 
     6121 TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 
     6181 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 
     6241 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 
     6301 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 
     6361 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 
     6421 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 
     6481 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 
     6541 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 
     6601 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 
     6661 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 
     6721 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     6781 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     6841 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     6901 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     6961 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     7021 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     7081 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     7141 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     7201 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     7261 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     7321 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     7381 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 
     7441 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 
     7501 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 
     7561 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 
     7621 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 
     7681 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 
     7741 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 
     7801 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 
     7861 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 
     7921 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 
     7981 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 
     8041 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 
     8101 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 
     8161 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 
     8221 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 
     8281 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 
     8341 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 
     8401 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 
     8461 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 
     8521 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 
     8581 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT 
     8641 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG 
     8701 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC 
     8761 AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC 
     8821 CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT 
     8881 TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA 
     8941 CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG 
     9001 CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//