LOCUS       RG214128                9023 bp    DNA     circular  SYN 
DEFINITION  CLCN3 (tGFP-tagged) - Human chloride channel 3 (CLCN3), transcript variant b
ACCESSION   NM_001829
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3504..4208 
                     /label="GFP"  
     misc_feature    4255..4840 
                     /label="PolyA signal"  
     misc_feature    6932..7577 
                     /label="ColE1"  
     misc_feature    5294..6088 
                     /label="Neo"  
     misc_feature    4925..5227 
                     /label="SV40 ori"  
     misc_feature    7592..8452 
                     /label="Amp"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3483..3489 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3483 
                     /label="CLCN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GAGTCTGAGC AGCTGTTCCA TAGAGGCTAC TATAGAAACA 
     1081 GCTACAACAG TATAACAAGT GCAAGTAGTG ATGAGGAACT TTTAGATGGA GCAGGTGTTA 
     1141 TTATGGACTT TCAAACATCT GAAGATGACA ATTTATTAGA TGGTGACACT GCAGTTGGAA 
     1201 CTCATTATAC AATGACAAAT GGAGGCAGCA TTAACAGTTC TACACATTTA CTGGATCTTT 
     1261 TGGATGAACC AATTCCAGGT GTTGGTACAT ATGATGATTT CCATACTATT GATTGGGTGC 
     1321 GAGAAAAATG TAAAGACAGA GAAAGGCATA GACGGATCAA CAGCAAAAAG AAAGAATCAG 
     1381 CATGGGAAAT GACAAAAAGT TTGTATGATG CGTGGTCAGG ATGGCTAGTA GTAACACTAA 
     1441 CAGGATTGGC ATCAGGGGCA CTGGCCGGAT TAATAGACAT TGCTGCCGAT TGGATGACTG 
     1501 ACCTAAAGGA GGGCATTTGC CTTAGTGCGT TGTGGTACAA CCACGAACAG TGCTGTTGGG 
     1561 GATCTAATGA AACAACATTT GAAGAGAGGG ATAAATGTCC ACAGTGGAAA ACATGGGCAG 
     1621 AATTAATCAT AGGTCAAGCA GAGGGTCCTG GTTCTTATAT CATGAACTAC ATAATGTACA 
     1681 TCTTCTGGGC CTTGAGTTTT GCCTTTCTTG CAGTTTCCCT GGTAAAGGTA TTTGCTCCAT 
     1741 ATGCCTGTGG CTCTGGAATT CCAGAGATTA AAACTATTTT AAGTGGATTC ATCATCAGAG 
     1801 GTTACTTGGG AAAATGGACT TTAATGATTA AAACCATCAC ATTAGTCCTG GCTGTGGCAT 
     1861 CAGGTTTGAG TTTAGGAAAA GAAGGTCCCC TGGTACATGT TGCCTGTTGC TGCGGAAATA 
     1921 TCTTTTCCTA CCTCTTTCCA AAGTATAGCA CAAACGAAGC TAAAAAAAGG GAGGTGCTAT 
     1981 CAGCTGCCTC AGCTGCAGGG GTTTCTGTAG CTTTTGGTGC ACCAATTGGA GGAGTTCTTT 
     2041 TTAGCCTGGA AGAGGTTAGC TATTATTTTC CTCTCAAAAC TTTATGGAGA TCATTTTTTG 
     2101 CTGCTTTAGT GGCTGCATTT GTTTTGAGGT CCATCAATCC ATTTGGTAAC AGCCGTCTGG 
     2161 TCCTTTTTTA TGTGGAGTAT CATACACCAT GGTACCTTTT TGAACTGTTT CCTTTTATTC 
     2221 TTCTAGGGGT ATTTGGAGGG CTTTGGGGAG CCTTTTTCAT TAGGGCAAAT ATTGCCTGGT 
     2281 GTCGTCGACG CAAGTCCACG AAATTTGGAA AGTATCCCGT TCTGGAAGTC ATTATTGTTG 
     2341 CAGCCATTAC TGCTGTGATA GCCTTCCCTA ATCCATACAC TAGGCTAAAC ACCAGTGAAC 
     2401 TGATCAAAGA GCTTTTTACA GACTGTGGTC CCCTGGAATC CTCTTCTCTT TGTGACTACA 
     2461 GAAATGACAT GAATGCCAGT AAAATTGTCG ATGACATTCC TGATCGTCCA GCAGGCATTG 
     2521 GAGTATATTC AGCTATATGG CAGTTATGCC TGGCACTCAT ATTTAAAATC ATAATGACAG 
     2581 TATTCACTTT TGGCATCAAG GTTCCATCAG GCTTGTTCAT CCCCAGCATG GCCATTGGAG 
     2641 CGATCGCAGG AAGGATTGTG GGGATTGCGG TGGAGCAGCT TGCCTACTAT CACCACGACT 
     2701 GGTTTATCTT TAAGGAGTGG TGTGAGGTCG GGGCTGATTG CATTACACCT GGCCTTTATG 
     2761 CCATGGTTGG TGCTGCTGCA TGCTTAGGTG GTGTGACAAG AATGACTGTC TCCCTGGTGG 
     2821 TTATTGTTTT TGAGCTTACT GGAGGCTTGG AATATATTGT TCCCCTTATG GCTGCAGTCA 
     2881 TGACCAGTAA ATGGGTTGGA GATGCCTTTG GCAGGGAAGG CATTTATGAA GCACACATCC 
     2941 GATTAAATGG ATACCCTTTC TTGGATGCAA AAGAAGAATT CACTCATACC ACCCTGGCTG 
     3001 CTGACGTTAT GAGACCTCGA AGGAATGATC CTCCCTTAGC TGTCCTGACA CAGGACAATA 
     3061 TGACAGTGGA TGATATAGAA AACATGATTA ATGAAACCAG CTACAATGGA TTTCCTGTCA 
     3121 TAATGTCAAA AGAATCTCAG AGATTAGTGG GATTTGCCCT CAGAAGAGAC CTGACAATTG 
     3181 CAATAGAAAG TGCCAGGAAA AAACAAGAAG GTATCGTTGG CAGTTCTCGG GTGTGTTTTG 
     3241 CACAGCACAC CCCATCTCTT CCAGCAGAAA GTCCTCGGCC ATTGAAGCTT CGAAGCATTC 
     3301 TTGACATGAG CCCTTTTACA GTGACAGACC ACACCCCAAT GGAGATCGTG GTGGATATTT 
     3361 TCCGAAAGCT GGGACTGAGG CAGTGCCTTG TAACTCACAA TGGGCGCCTC CTTGGCATTA 
     3421 TAACAAAAAA AGATATCCTC CGGCATATGG CCCAGACGGC AAACCAAGAC CCCGCTTCAA 
     3481 TAATGTTCAA CACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3541 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3601 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3661 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     3721 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     3781 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     3841 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     3901 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     3961 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4021 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4081 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4141 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4201 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4261 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4321 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4381 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4441 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4501 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4561 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4621 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4681 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4741 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4801 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4861 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     4921 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     4981 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5041 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5101 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5161 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5221 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5281 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5341 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5401 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5461 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5521 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5581 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5641 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     5701 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     5761 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     5821 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     5881 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     5941 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6001 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6061 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6121 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6181 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6241 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6301 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6361 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6421 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6481 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6541 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6601 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6661 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     6721 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     6781 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     6841 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     6901 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     6961 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7021 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7081 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7141 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7201 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7261 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7321 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7381 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7441 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7501 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7561 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7621 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7681 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     7741 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     7801 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     7861 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     7921 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     7981 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8041 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8101 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8161 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8221 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8281 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8341 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8401 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8461 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8521 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8581 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8641 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     8701 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     8761 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     8821 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     8881 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     8941 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9001 AACAAAAATT TAACGCGAAT TTT
//