LOCUS       RG213786                7978 bp    DNA     circular  SYN 
DEFINITION  CATSPER4 (tGFP-tagged) - Human cation channel, sperm associated 4 (CATSPER4)
ACCESSION   NM_198137
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2459..3163 
                     /label="GFP"  
     misc_feature    3210..3795 
                     /label="PolyA signal"  
     misc_feature    5887..6532 
                     /label="ColE1"  
     misc_feature    4249..5043 
                     /label="Neo"  
     misc_feature    3880..4182 
                     /label="SV40 ori"  
     misc_feature    6547..7407 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2438..2444 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2438 
                     /label="CATSPER4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGATAAT GAAAAGGCCT GGTGGCAGCA ATGGACCTCC CATACAGGCC 
     1081 TCGAGGGGTG GGGCGGGACT CAGGAGGACC GTATGGGGTT TGGAGGGGCA GTAGCTGCAC 
     1141 TGAGGGGCCG CCCCTCTCCC CTGCAGAGTA CCATTCACGA GTCCTACGGT CGGCCAGAGG 
     1201 AGCAAGTGCT CATCAACCGC CAGGAAATCA CGAACAAAGC GGACGCCTGG GACATGCAGG 
     1261 AGTTCATCAC TCACATGTAC ATCAAGCAGC TGCTCCGACA CCCCGCCTTC CAACTGCTGC 
     1321 TGGCCCTGCT GCTGGTGATC AATGCCATCA CCATCGCTCT CCGTACCAAC TCCTACCTGG 
     1381 ACCAGAAACA CTATGAGTTG TTCTCTACCA TAGATGACAT TGTGCTGACC ATCCTTCTTT 
     1441 GTGAGGTTCT CCTTGGCTGG CTCAATGGCT TCTGGATTTT CTGGAAGGAC GGCTGGAACA 
     1501 TCCTCAACTT CATTATCGTC TTTATCTTGC TCTTGCGGTT CTTCATTAAT GAAATCAATA 
     1561 TTCCCTCCAT CAACTACACT CTCAGGGCGC TTCGTCTGGT GCATGTGTGC ATGGCGGTGG 
     1621 AGCCCCTCGC CCGGATCATC CGCGTCATCC TGCAGTCGGT GCCTGACATG GCCAATATCA 
     1681 TGGTCCTCAT CCTCTTCTTC ATGCTGGTTT TTTCCGTGTT TGGAGTAACA CTCTTTGGTG 
     1741 CATTCGTGCC CAAGCATTTC CAGAACATAC AGGTTGCGCT GTACACCCTC TTCATCTGCA 
     1801 TCACCCAGGA CGGCTGGGTG GACATCTACA GTGACTTCCA GACAGAGAAG AGGGAATATG 
     1861 CAATGGAGAT TGGGGGTGCC ATCTACTTTA CCATCTTCAT CACCATCGGT GCCTTCATTG 
     1921 GCATCAACCT GTTCGTCATC GTGGTGACCA CCAACCTGGA GCAAATGATG AAGGCAGGAG 
     1981 AGCAGGGACA ACAGCAACGA ATAACCTTTA GTGAGACAGG CGCAGAGGAA GAGGAGGAGA 
     2041 ATGACCAGCT GCCACTGGTG CATTGTGTGG TCGCCCGCTC GGAGAAATCT GGTCTCCTCC 
     2101 AGGAACCCCT TGCGGGAGGC CCCCTGTCGA ACCTCTCAGA AAACACGTGT GACAACTTTT 
     2161 GCTTGGTGCT TGAGGCAATA CAGGAGAACC TGAGGCAGTA CAAGGAGATC CGAGATGAAC 
     2221 TCAACATGAT TGTGGAGGAG GTGCGCGCAA TCCGCTTCAA CCAGGAGCAG GAGTCAGAGG 
     2281 TGTTGAACAG GCGCTCGTCG ACGAGCGGGT CGTTGGAGAC TACGTCATCC AAGGACATCC 
     2341 GCCAGATGTC TCAACAGCAA GACTTGCTCA GTGCGCTCGT TAGCATGGAA AAGGTTCATG 
     2401 ACTCTAGCTC ACAAATACTC CTTAAAAAAC ACAAGAGCAG CCACTGACGC GTACGCGGCC 
     2461 GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT GCCGCATCAC 
     2521 CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA CCCCCGAGCA 
     2581 GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA GCCCCTACCT 
     2641 GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA GCGGCTACGA 
     2701 GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA TCGAGAAGTA 
     2761 CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG GCCGCGTGAT 
     2821 CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT TCACCGACAA 
     2881 GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA ACGATCTGGA 
     2941 TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT CCGTGGTGGA 
     3001 CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG GGGGCCCCAT 
     3061 GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA TCGTGGAGTA 
     3121 CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT AAACGGCCGG 
     3181 CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG 
     3241 TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT 
     3301 TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG 
     3361 TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG 
     3421 AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC 
     3481 AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC 
     3541 TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT 
     3601 CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG 
     3661 CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG 
     3721 ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT 
     3781 TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA 
     3841 CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC 
     3901 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT 
     3961 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 
     4021 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 
     4081 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG 
     4141 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC 
     4201 CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA 
     4261 TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG 
     4321 GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG 
     4381 CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC 
     4441 AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC 
     4501 TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG 
     4561 ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC 
     4621 GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA 
     4681 TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG 
     4741 AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG 
     4801 GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG 
     4861 GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC TATCAGGACA 
     4921 TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC 
     4981 TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG 
     5041 ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA CGCCCAACCT 
     5101 GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT TCGGAATCGT 
     5161 TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG AGTTCTTCGC 
     5221 CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA 
     5281 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA 
     5341 TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT AATCATGGTC 
     5401 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 
     5461 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 
     5521 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 
     5581 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 
     5641 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6361 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6421 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6481 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6541 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT 
     6601 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG 
     6661 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA 
     6721 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT 
     6781 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT 
     6841 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT 
     6901 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT 
     6961 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC 
     7021 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC 
     7081 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT 
     7141 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG 
     7201 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT 
     7261 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC 
     7321 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA 
     7381 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG 
     7441 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA 
     7501 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG 
     7561 CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG 
     7621 CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT 
     7681 TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA 
     7741 CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA 
     7801 GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA 
     7861 AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC 
     7921 GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//