LOCUS       RG213553                9485 bp    DNA     circular  SYN 
DEFINITION  PCSK6 (tGFP-tagged) - Human proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 7
ACCESSION   NM_138320
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3966..4670 
                     /label="GFP"  
     misc_feature    4717..5302 
                     /label="PolyA signal"  
     misc_feature    7394..8039 
                     /label="ColE1"  
     misc_feature    5756..6550 
                     /label="Neo"  
     misc_feature    5387..5689 
                     /label="SV40 ori"  
     misc_feature    8054..8914 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3945..3951 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3945 
                     /label="PCSK6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCGCGC GCGCCGCCTG CGCCCGGGCC CCGGCCGCCG CCCCGGGCCG 
     1081 CCGCCGCCAC CGACACCGCC GCGGGCGCGG GGGGCGCGGG GGGCGCGGGG GGCGCCGGCG 
     1141 GGCCCGGGTT CCGGCCGCTC GCGCCGCGTC CCTGGCGCTG GCTGCTGCTG CTGGCGCTGC 
     1201 CTGCCGCCTG CTCCGCGCCC CCGCCGCGCC CCGTCTACAC CAACCACTGG GCGGTGCAAG 
     1261 TGCTGGGCGG CCCGGCCGAG GCGGACCGCG TGGCGGCGGC GCACGGGTAC CTCAACTTGG 
     1321 GCCAGATTGG AAACCTGGAA GATTACTACC ATTTTTATCA CAGCAAAACC TTTAAAAGAT 
     1381 CAACCTTGAG TAGCAGAGGC CCTCACACCT TCCTCAGAAT GGACCCCCAG GTGAAATGGC 
     1441 TCCAGCAACA GGAAGTGAAA CGAAGGGTGA AGAGACAGGT GCGAAGTGAC CCGCAGGCCC 
     1501 TTTACTTCAA CGACCCCATT TGGTCCAACA TGTGGTACCT GCATTGTGGC GACAAGAACA 
     1561 GTCGCTGCCG GTCGGAAATG AATGTCCAGG CAGCGTGGAA GAGGGGCTAC ACAGGAAAAA 
     1621 ACGTGGTGGT CACCATCCTT GATGATGGCA TAGAGAGAAA TCACCCTGAC CTGGCCCCAA 
     1681 ATTATGATTC CTACGCCAGC TACGACGTGA ACGGCAATGA TTATGACCCA TCTCCACGAT 
     1741 ATGATGCCAG CAATGAAAAT AAACACGGCA CTCGTTGTGC GGGAGAAGTT GCTGCTTCAG 
     1801 CAAACAATTC CTACTGCATC GTGGGCATAG CGTACAATGC CAAAATAGGA GGCATCCGCA 
     1861 TGCTGGACGG CGATGTCACA GATGTGGTCG AGGCAAAGTC GCTGGGCATC AGACCCAACT 
     1921 ACATCGACAT TTACAGTGCC AGCTGGGGGC CGGACGACGA CGGCAAGACG GTGGACGGGC 
     1981 CCGGCCGACT GGCTAAGCAG GCTTTCGAGT ATGGCATTAA AAAGGGCCGG CAGGGCCTGG 
     2041 GCTCCATTTT CGTCTGGGCA TCTGGGAATG GCGGGAGAGA GGGGGACTAC TGCTCGTGCG 
     2101 ATGGCTACAC CAACAGCATC TACACCATCT CCGTCAGCAG CGCCACCGAG AATGGCTACA 
     2161 AGCCCTGGTA CCTGGAAGAG TGTGCCTCCA CCCTGGCCAC CACCTACAGC AGTGGGGCCT 
     2221 TTTATGAGCG AAAAATCGTC ACCACGGATC TGCGTCAGCG CTGTACCGAT GGCCACACTG 
     2281 GGACCTCAGT CTCTGCCCCC ATGGTGGCGG GCATCATCGC CTTGGCTCTA GAAGCAAACA 
     2341 GCCAGTTAAC CTGGAGGGAC GTCCAGCACC TGCTAGTGAA GACATCCCGG CCGGCCCACC 
     2401 TGAAAGCGAG CGACTGGAAA GTGAACGGCG CGGGTCATAA AGTTAGCCAT TTCTATGGAT 
     2461 TTGGTTTGGT GGACGCAGAA GCTCTCGTTG TGGAGGCAAA GAAGTGGACA GCAGTGCCAT 
     2521 CGCAGCACAT GTGTGTGGCC GCCTCGGACA AGAGACCCAG GAGCATCCCC TTAGTGCAGG 
     2581 TGCTGCGGAC TACGGCCCTG ACCAGCGCCT GCGCGGAGCA CTCGGACCAG CGGGTGGTCT 
     2641 ACTTGGAGCA CGTGGTGGTT CGCACCTCCA TCTCACACCC ACGCCGAGGA GACCTCCAGA 
     2701 TCTACCTGGT TTCTCCCTCG GGAACCAAGT CTCAACTTCT GGCAAAGAGG TTGCTGGATC 
     2761 TTTCCAATGA AGGGTTTACA AACTGGGAAT TCATGACTGT CCACTGCTGG GGAGAAAAGG 
     2821 CTGAAGGGCA GTGGACCTTG GAAATCCAAG ATCTGCCATC CCAGGTCCGC AACCCGGAGA 
     2881 AGCAAGGGAA GTTGAAAGAA TGGAGCCTCA TACTGTATGG CACAGCAGAG CACCCGTACC 
     2941 ACACCTTCAG TGCCCATCAG TCCCGCTCGC GGATGCTGGA GCTCTCAGCC CCAGAGCTGG 
     3001 AGCCACCCAA GGCTGCCCTG TCACCCTCCC AGGTGGAAGT TCCTGAAGAT GAGGAAGATT 
     3061 ACACAGCTCA ATCCACCCCA GGCTCTGCTA ATATTTTACA GACCAGTGTG TGCCATCCGG 
     3121 AGTGTGGTGA CAAAGGCTGT GATGGCCCCA ATGCAGACCA GTGCTTGAAC TGCGTCCACT 
     3181 TCAGCCTGGG GAGTGTCAAG ACCAGCAGGA AGTGCGTGAG TGTGTGCCCC TTGGGCTACT 
     3241 TTGGGGACAC AGCAGCAAGA CGCTGTCGCC GGTGCCACAA GGGGTGTGAG ACCTGCTCCA 
     3301 GCAGAGCTGC GACGCAGTGC CTGTCTTGCC GCCGCGGGTT CTATCACCAC CAGGAGATGA 
     3361 ACACCTGTGT GACCCTCTGT CCTGCAGGAT TTTATGCTGA TGAAAGTCAG AAAAATTGCC 
     3421 TTAAATGCCA CCCAAGCTGT AAAAAGTGCG TGGATGAACC TGAGAAATGT ACTGTCTGTA 
     3481 AAGAAGGATT CAGCCTTGCA CGGGGCAGCT GCATTCCTGA CTGTGAGCCA GGCACCTACT 
     3541 TTGACTCAGA GCTGATCAGA TGTGGGGAAT GCCATCACAC CTGCGGAACC TGCGTGGGGC 
     3601 CAGGCAGAGA AGAGTGCATT CACTGTGCGA AAAACTTCCA CTTCCACGAC TGGAAGTGTG 
     3661 TGCCAGCCTG TGGTGAGGGC TTCTACCCAG AAGAGATGCC GGGCTTGCCC CACAAAGTGT 
     3721 GTCGAAGGTA CGGTCCTCCT GGCGGGGAAC GGCAGGCAGC TGTGTCCAGC AAAGGAGTGC 
     3781 CTGGAGGGCA GAGTCTGGCA GCATCTTCCC CAGGGGCCGG GGAGGGCATG TTGCATCACC 
     3841 CCACTGTGGA CAGGAGCCCA TTTACAGAGC TGCTGAGGGG CCTTCGTCCC TTTGTTCACT 
     3901 GGATGCATAT TTGTTGGGTC CCTGCTGTGG GCAGACACCG TGCAGCAGCG GGAACGCGTA 
     3961 CGCGGCCGCT CGAGATGGAG AGCGACGAGA GCGGCCTGCC CGCCATGGAG ATCGAGTGCC 
     4021 GCATCACCGG CACCCTGAAC GGCGTGGAGT TCGAGCTGGT GGGCGGCGGA GAGGGCACCC 
     4081 CCGAGCAGGG CCGCATGACC AACAAGATGA AGAGCACCAA AGGCGCCCTG ACCTTCAGCC 
     4141 CCTACCTGCT GAGCCACGTG ATGGGCTACG GCTTCTACCA CTTCGGCACC TACCCCAGCG 
     4201 GCTACGAGAA CCCCTTCCTG CACGCCATCA ACAACGGCGG CTACACCAAC ACCCGCATCG 
     4261 AGAAGTACGA GGACGGCGGC GTGCTGCACG TGAGCTTCAG CTACCGCTAC GAGGCCGGCC 
     4321 GCGTGATCGG CGACTTCAAG GTGATGGGCA CCGGCTTCCC CGAGGACAGC GTGATCTTCA 
     4381 CCGACAAGAT CATCCGCAGC AACGCCACCG TGGAGCACCT GCACCCCATG GGCGATAACG 
     4441 ATCTGGATGG CAGCTTCACC CGCACCTTCA GCCTGCGCGA CGGCGGCTAC TACAGCTCCG 
     4501 TGGTGGACAG CCACATGCAC TTCAAGAGCG CCATCCACCC CAGCATCCTG CAGAACGGGG 
     4561 GCCCCATGTT CGCCTTCCGC CGCGTGGAGG AGGATCACAG CAACACCGAG CTGGGCATCG 
     4621 TGGAGTACCA GCACGCCTTC AAGACCCCGG ATGCAGATGC CGGTGAAGAA AGAGTTTAAA 
     4681 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4741 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4801 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4861 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4921 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4981 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5041 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5101 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5161 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5221 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5281 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5341 TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 
     5401 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 
     5461 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 
     5521 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 
     5581 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 
     5641 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 
     5701 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 
     5761 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 
     5821 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 
     5881 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 
     5941 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 
     6001 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 
     6061 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 
     6121 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 
     6181 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 
     6241 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 
     6301 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 
     6361 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC CGACCGCTAT 
     6421 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 
     6481 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 
     6541 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 
     6601 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 
     6661 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 
     6721 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 
     6781 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 
     6841 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 
     6901 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 
     6961 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 
     7021 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 
     7081 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 
     7141 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 
     7201 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 
     7261 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 
     7321 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 
     7381 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 
     7441 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 
     7501 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 
     7561 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 
     7621 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 
     7681 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 
     7741 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 
     7801 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC 
     7861 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 
     7921 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA 
     7981 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT 
     8041 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA 
     8101 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 
     8161 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG 
     8221 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG 
     8281 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT 
     8341 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT 
     8401 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 
     8461 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA 
     8521 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA 
     8581 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT 
     8641 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC 
     8701 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 
     8761 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT 
     8821 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG 
     8881 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT 
     8941 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT 
     9001 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC 
     9061 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 
     9121 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 
     9181 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT 
     9241 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 
     9301 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 
     9361 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 
     9421 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 
     9481 ATTTT
//