LOCUS       RG212424                9299 bp    DNA     circular  SYN 
DEFINITION  EPB41L4B (tGFP-tagged) - Human erythrocyte membrane protein band 4.1 like 4B (EPB41L4B), transcript variant 2
ACCESSION   NM_019114
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3780..4484 
                     /label="GFP"  
     misc_feature    4531..5116 
                     /label="PolyA signal"  
     misc_feature    7208..7853 
                     /label="ColE1"  
     misc_feature    5570..6364 
                     /label="Neo"  
     misc_feature    5201..5503 
                     /label="SV40 ori"  
     misc_feature    7868..8728 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3759..3765 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3759 
                     /label="EPB41L4B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCGCTAC GCGGGGCGCG GCGGGGCGCG GGGCCGCCGG GCTGGGGGAC 
     1081 GAGCGCGATG GGGGCCACGG GGGGGCCCGG CCGCCGCCGC CTCCTCCTCG GCGCTGCCCG 
     1141 CCGCGCCCGG GGGCAGCGTG TTCCCGGCGG GCGGCGGGCC CCTGCTCACC GGCGGCGCGG 
     1201 CCGTGCACAT CTCCGCCGCC GGCGCCGCCA AGGCCACCCT CTACTGCCGC GTCTTCCTGC 
     1261 TCGACGGGAC CGAAGTGAGC GTGGACCTGC CGAAACATGC CAAAGGCCAG GATTTGTTTG 
     1321 ATCAGATTGT GTACCACTTG GACCTTGTGG AAACAGATTA CTTTGGCCTC CAGTTCCTCG 
     1381 ACTCTGCCCA GGTTGCGCAC TGGCTGGATC ATGCCAAACC CATAAAAAAG CAGATGAAAA 
     1441 TTGGACCTGC TTATGCTTTA CACTTTCGAG TTAAATACTA TTCTTCAGAA CCAAACAACC 
     1501 TTCGTGAGGA GTTTACAAGG TACCTGTTTG TTTTACAACT CAGGCATGAC ATTCTTTCTG 
     1561 GAAAATTGAA ATGCCCTTAT GAAACAGCTG TGGAATTAGC TGCTCTCTGT CTACAAGCGG 
     1621 AGCTTGGGGA GTGCGAGCTT CCAGAACACA CACCAGAGCT TGTGTCTGAG TTTCGGTTCA 
     1681 TTCCAAATCA GACAGAAGCA ATGGAATTTG ATATCTTCCA GAGATGGAAA GAGTGCAGGG 
     1741 GAAAGAGCCC TGCCCAGGCG GAACTCTCCT ATCTGAATAA AGCGAAGTGG CTGGAAATGT 
     1801 ATGGGGTAGA CATGCACGTT GTCAGGGGAA GAGATGGCTG TGAATATTCT CTTGGACTGA 
     1861 CCCCGACAGG CATATTAATC TTTGAAGGAG CTAACAAAAT AGGCTTATTC TTTTGGCCTA 
     1921 AAATTACCAA AATGGATTTT AAAAAGAGCA AATTGACACT CGTGGTGGTC GAGGATGATG 
     1981 ATCAGGGACG TGAGCAAGAG CACACGTTTG TGTTCCGGTT AGACAGTGCC AGGACCTGCA 
     2041 AACACCTTTG GAAGTGTGCA GTTGAGCACC ACGCATTCTT CCGACTGCGG ACGCCAGGAA 
     2101 ACAGCAAATC CAATAGATCC GACTTTATCA GGCTGGGCTC TCGCTTCAGA TTCAGTGGGC 
     2161 GGACAGAATA TCAAGCTACA CATGGCTCCA GGTTACGAAG AACCAGCACC TTTGAGAGGA 
     2221 AGCCTAGTAA ACGTTATCCA TCCCGGAGAC ATTCAACGTT CAAAGCAAGC AACCCAGTGA 
     2281 TAGCAGCCCA GCTCTGCTCT AAAACAAATC CGGAAGTCCA TAATTACCAG CCTCAATATC 
     2341 ATCCTAATAT CCATCCCAGC CAGCCCCGGT GGCATCCTCA CTCTCCAAAT GTCAGCTACC 
     2401 CGCTCCCTTC CCCAGTGCTT AGCAGCTCGG ACCGGTTGCC TTTTGGCATT GAGGAGAATG 
     2461 GGGGCACACC GTTCCTCACC GCAGCTTCAG GAAGGCATCA CCACCAGCAC CAGCATCAGC 
     2521 ATCAGCACCA GCACCACTCA AACTACAGCC TCTCACTGAC CCTGGAGAAC AAAGAGGGGC 
     2581 CTCTGAGGTC CCCAAACTCC AGCAGCAAGT CCCTTACAAA ACTGAGTCCA GGAACACCTG 
     2641 CCTTGTTCAG TGAAGCCGCT GCCCATCTAA AGAAGCTGGA ACTGGAAACT GTGAAGGCTG 
     2701 CTGGACCCTG GCCTCCTCTG CACATCAACA TAAACAAGGC TGAAGAAAAG AAAGTCTCGG 
     2761 AGAAAACTCT TCAGACTCCA CTTTTGCCTT CCCCTGTTGC GGATCATGTG AAGTGTAACA 
     2821 TTCTGAAAGC CCAGTTGGAA AATGCTTCCC GAGTGAACAT CCAGGGTGGA AAGGAGGAAT 
     2881 CACCGTTTGT AAATATCAAT AAGAAATCCA GTCTTCAGGA CGCTAGTGTA AGAAGTCCTA 
     2941 TTCCTATTCG TGTGGAAACT GCCCAGCCAG CTGTGGAAAA GCCGGAAATC AAGCCTCCCC 
     3001 GAGTGAGGAA GTTAACAAGA CAGTATAGTT TTGATGAAGA CGACCTCCCT CCAGACCTGG 
     3061 CCGAGGCAGT GGGAGTGACC ACATCTACAA CCACAAACAC CACAACGGCT GCCACACAAG 
     3121 TCTCCGTGCC GCTGCCGTCC CCCAAGGTCC AGAATGTCAG CTCGCCTCAC AAGTCAGAAG 
     3181 GCAAAGGCCT GCTGTCCCCT GGGGCCAAGA GCCCCTCTGA CCGAGGAGGT GCCTTTACCC 
     3241 TGGAGCCGGG TGATCTTCTG ATGGATTTCA CAGAAGCCAC TCCTCTGGCA GAGCCCGCCA 
     3301 GCAACCCCCA CTGTGCCCAC TCTCGCTGTT CTCCTCCACT CTCTCTCCCC ATGAAGGAAG 
     3361 AGACCACTGG AGTTTGCATG TACCCTCCAA TCAAAACGAG GCTGATAAAA ACATTCCCGG 
     3421 TTGATACAAT GACCCCGTTT CCTGATACTT TCACCACAGG GCCACAGTTT ACTGCAGACT 
     3481 TCAGAGACAG TAAATTACAG TGCTGTCCTG GCCCGACTTC CCCGCTGATC CCAGCAGCGA 
     3541 CCCTGAGGCC TTTGACAGAG ACCGTCTCCA CAGTGCAGAC CATTTACACC ACCCCGGAAA 
     3601 CCTGTTTCTC TGGCAGCCAG TGCAGAGACA CTCCGGCAGG AACTGGAGAG AGAGAAGATG 
     3661 ATGAAAAGAC TGTTGATGAC CAGACTGTGA AATTCTCCCC TTGTCACCTG GAAGATGGCA 
     3721 TGGTGCCTTC TGTCCATCTT CTTTCTTCGG GCTTTGTGTG CTCACTCACG CGTACGCGGC 
     3781 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3841 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3901 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3961 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     4021 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     4081 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4141 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4201 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4261 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4321 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4381 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4441 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4501 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4561 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4621 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4681 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4741 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4801 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4861 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4921 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4981 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5041 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5101 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5161 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5221 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5281 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5341 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5401 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5461 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5521 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5581 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5641 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5701 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5761 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5821 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5881 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5941 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     6001 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     6061 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6121 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6181 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6241 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6301 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6361 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6421 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6481 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6541 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6601 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6661 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6721 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6781 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6841 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6901 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6961 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     7021 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     7081 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7141 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7201 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7261 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7321 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7381 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7441 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7501 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7561 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7621 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7681 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7741 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7801 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7861 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7921 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7981 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     8041 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     8101 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8161 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8221 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8281 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8341 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8401 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8461 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8521 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8581 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8641 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8701 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8761 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8821 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8881 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8941 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     9001 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     9061 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9121 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9181 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9241 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//