LOCUS       RG211796                9179 bp    DNA     circular  SYN 
DEFINITION  VLDLR (tGFP-tagged) - Human very low density lipoprotein receptor (VLDLR), transcript variant 1
ACCESSION   NM_003383
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3660..4364 
                     /label="GFP"  
     misc_feature    4411..4996 
                     /label="PolyA signal"  
     misc_feature    7088..7733 
                     /label="ColE1"  
     misc_feature    5450..6244 
                     /label="Neo"  
     misc_feature    5081..5383 
                     /label="SV40 ori"  
     misc_feature    7748..8608 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3639..3645 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3639 
                     /label="VLDLR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCACGTCC GCGCTCTGGG CGCTCTGGCT GCTGCTCGCG CTGTGCTGGG 
     1081 CGCCCCGGGA GAGCGGCGCC ACCGGAACCG GGAGAAAAGC CAAATGTGAA CCCTCCCAAT 
     1141 TCCAGTGCAC AAATGGTCGC TGTATTACGC TGTTGTGGAA ATGTGATGGG GATGAAGACT 
     1201 GTGTTGACGG CAGTGATGAA AAGAACTGTG TAAAGAAGAC GTGTGCTGAA TCTGACTTCG 
     1261 TGTGCAACAA TGGCCAGTGT GTTCCCAGCC GATGGAAGTG TGATGGAGAT CCTGACTGCG 
     1321 AAGATGGTTC AGATGAAAGC CCAGAACAGT GCCATATGAG AACATGCCGC ATACATGAAA 
     1381 TCAGCTGTGG CGCCCATTCT ACTCAGTGTA TCCCAGTGTC CTGGAGATGT GATGGTGAAA 
     1441 ATGATTGTGA CAGTGGAGAA GATGAAGAAA ACTGTGGCAA TATAACATGT AGTCCCGACG 
     1501 AGTTCACCTG CTCCAGTGGC CGCTGCATCT CCAGGAACTT TGTATGCAAT GGCCAGGATG 
     1561 ACTGCAGCGA TGGCAGTGAT GAGCTGGACT GTGCCCCGCC AACCTGTGGC GCCCATGAGT 
     1621 TCCAGTGCAG CACCTCCTCC TGCATCCCCA TCAGCTGGGT ATGCGACGAT GATGCAGACT 
     1681 GCTCCGACCA ATCTGATGAG TCCCTGGAGC AGTGTGGCCG TCAGCCAGTC ATACACACCA 
     1741 AGTGTCCAGC CAGCGAAATC CAGTGCGGCT CTGGCGAGTG CATCCATAAG AAGTGGCGAT 
     1801 GTGATGGGGA CCCTGACTGC AAGGATGGCA GTGATGAGGT CAACTGTCCC TCTCGAACTT 
     1861 GCCGACCTGA CCAATTTGAA TGTGAGGATG GCAGCTGCAT CCATGGCAGC AGGCAGTGTA 
     1921 ATGGTATCCG AGACTGTGTC GATGGTTCCG ATGAAGTCAA CTGCAAAAAT GTCAATCAGT 
     1981 GCTTGGGCCC TGGAAAATTC AAGTGCAGAA GTGGAGAATG CATAGATATC AGCAAAGTAT 
     2041 GTAACCAGGA GCAGGACTGC AGGGACTGGA GTGATGAGCC CCTGAAAGAG TGTCATATAA 
     2101 ACGAATGCTT GGTAAATAAT GGTGGATGTT CTCATATCTG CAAAGACCTA GTTATAGGCT 
     2161 ACGAGTGTGA CTGTGCAGCT GGGTTTGAAC TGATAGATAG GAAAACCTGT GGAGATATTG 
     2221 ATGAATGCCA AAATCCAGGA ATCTGCAGTC AAATTTGTAT CAACTTAAAA GGCGGTTACA 
     2281 AGTGTGAATG TAGTCGTGGC TATCAAATGG ATCTTGCTAC TGGCGTGTGC AAGGCAGTAG 
     2341 GCAAAGAGCC AAGTCTGATC TTCACTAATC GAAGAGACAT CAGGAAGATT GGCTTAGAGA 
     2401 GGAAAGAATA TATCCAACTA GTTGAACAGC TAAGAAACAC TGTGGCTCTC GATGCTGACA 
     2461 TTGCTGCCCA GAAACTATTC TGGGCCGATC TAAGCCAAAA GGCTATCTTC AGTGCCTCAA 
     2521 TTGATGACAA GGTTGGTAGA CATGTTAAAA TGATCGACAA TGTCTATAAT CCTGCAGCCA 
     2581 TTGCTGTTGA TTGGGTGTAC AAGACCATCT ACTGGACTGA TGCGGCTTCT AAGACTATTT 
     2641 CAGTAGCTAC CCTAGATGGA ACCAAGAGGA AGTTCCTGTT TAACTCTGAC TTGCGAGAGC 
     2701 CTGCCTCCAT AGCTGTGGAC CCACTGTCTG GCTTTGTTTA CTGGTCAGAC TGGGGTGAAC 
     2761 CAGCTAAAAT AGAAAAAGCA GGAATGAATG GATTCGATAG ACGTCCACTG GTGACAGCGG 
     2821 ATATCCAGTG GCCTAACGGA ATTACACTTG ACCTTATAAA AAGTCGCCTC TATTGGCTTG 
     2881 ATTCTAAGTT GCACATGTTA TCCAGCGTGG ACTTGAATGG CCAAGATCGT AGGATAGTAC 
     2941 TAAAGTCTCT GGAGTTCCTA GCTCATCCTC TTGCACTAAC AATATTTGAG GATCGTGTCT 
     3001 ACTGGATAGA TGGGGAAAAT GAAGCAGTCT ATGGTGCCAA TAAATTCACT GGATCAGAGC 
     3061 TAGCCACTCT AGTCAACAAC CTGAATGATG CCCAAGACAT CATTGTCTAT CATGAACTTG 
     3121 TACAGCCATC AGGTAAAAAT TGGTGTGAAG AAGACATGGA GAATGGAGGA TGTGAATACC 
     3181 TATGCCTGCC AGCACCACAG ATTAATGATC ACTCTCCAAA ATATACCTGT TCCTGTCCCA 
     3241 GTGGGTACAA TGTAGAGGAA AATGGCCGAG ACTGTCAAAG TACTGCAACT ACTGTGACTT 
     3301 ACAGTGAGAC AAAAGATACG AACACAACAG AAATTTCAGC AACTAGTGGA CTAGTTCCTG 
     3361 GAGGGATCAA TGTGACCACA GCAGTATCAG AGGTCAGTGT TCCCCCAAAA GGGACTTCTG 
     3421 CCGCATGGGC CATTCTTCCT CTCTTGCTCT TAGTGATGGC AGCAGTAGGT GGCTACTTGA 
     3481 TGTGGCGGAA TTGGCAACAC AAGAACATGA AAAGCATGAA CTTTGACAAT CCTGTGTACT 
     3541 TGAAAACCAC TGAAGAGGAC CTCTCCATAG ACATTGGTAG ACACAGTGCT TCTGTTGGAC 
     3601 ACACGTACCC AGCAATATCA GTTGTAAGCA CAGATGATGA TCTAGCTACG CGTACGCGGC 
     3661 CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA 
     3721 CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC 
     3781 AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC 
     3841 TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG 
     3901 AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT 
     3961 ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA 
     4021 TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA 
     4081 AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG 
     4141 ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG 
     4201 ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA 
     4261 TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT 
     4321 ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGTT TAAACGGCCG 
     4381 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4441 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4501 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4561 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4621 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4681 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4741 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4801 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4861 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4921 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4981 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5041 ACGCGGCCAG CTTGGCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 
     5101 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 
     5161 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 
     5221 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 
     5281 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT 
     5341 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 
     5401 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 
     5461 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 
     5521 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 
     5581 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 
     5641 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 
     5701 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 
     5761 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 
     5821 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 
     5881 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 
     5941 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 
     6001 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 
     6061 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCCGACCG CTATCAGGAC 
     6121 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 
     6181 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 
     6241 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC 
     6301 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 
     6361 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 
     6421 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 
     6481 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 
     6541 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 
     6601 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 
     6661 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 
     6721 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 
     6781 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 
     6841 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 
     6901 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 
     6961 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 
     7021 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 
     7081 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 
     7141 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 
     7201 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 
     7261 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 
     7321 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 
     7381 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 
     7441 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 
     7501 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA 
     7561 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 
     7621 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 
     7681 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 
     7741 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 
     7801 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 
     7861 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 
     7921 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 
     7981 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 
     8041 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 
     8101 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 
     8161 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 
     8221 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 
     8281 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 
     8341 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 
     8401 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 
     8461 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 
     8521 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 
     8581 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 
     8641 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     8701 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     8761 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     8821 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     8881 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     8941 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     9001 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     9061 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     9121 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//