LOCUS       RG211665                9323 bp    DNA     circular  SYN 
DEFINITION  KSR2 (tGFP-tagged) - Human kinase suppressor of ras 2 (KSR2)
ACCESSION   NM_173598
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3804..4508 
                     /label="GFP"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    7232..7877 
                     /label="ColE1"  
     misc_feature    5594..6388 
                     /label="Neo"  
     misc_feature    5225..5527 
                     /label="SV40 ori"  
     misc_feature    7892..8752 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3783..3789 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3783 
                     /label="KSR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGACTTG AGCATCTCCA ACCTGGAAGG GCTTAGGACC AAATGTGCTA 
     1081 CCTCCAACGA CCTCACACAA AAAGAAATCC GGACCCTGGA GAGCAAGCTG GTGAAGTACT 
     1141 TCAGCCGGCA GCTGTCCTGC AAAAAGAAGG TAGCCTTGCA GGAGCGCAAC GCGGAGCTGG 
     1201 ACGGCTTCCC CCAGCTACGG CACTGGTTCC GAATCGTCGA TGTGCGCAAG GAGGTCCTGG 
     1261 AGGAAATCTC CCCCGGCCAG CTGAGCCTGG AGGACCTCTT GGAGATGACG GATGAACAGG 
     1321 TGTGCGAGAC TGTGGAGAAA TACGGAGCCA ACCGGGAGGA GTGTGCCCGC CTCAACGCCT 
     1381 CCCTCTCCTG CCTCAGGAAT GTCCACATGT CAGGAGGCAA CCTTTCCAAA CAAGACTGGA 
     1441 CCATCCAGTG GCCCACGACA GAGACGGGGA AGGAGAACAA TCCCGTGTGC CCCCCGGAGC 
     1501 CCACCCCGTG GATCCGCACC CATCTCTCCC AGAGCCCCAG GGTCCCGTCC AAGTGCGTCC 
     1561 AGCACTATTG TCACACCAGC CCCACTCCCG GGGCCCCTGT GTACACCCAC GTGGACAGGC 
     1621 TTACCGTGGA CGCCTACCCG GGCTTGTGCC CGCCCCCGCC ACTGGAGTCG GGCCACCGTT 
     1681 CCCTGCCCCC ATCGCCCCGG CAGCGGCACG CGGTCCGCAC CCCGCCGCGC ACCCCCAACA 
     1741 TCGTCACCAC CGTGACCCCG CCGGGCACGC CGCCCATGAG GAAGAAGAAC AAGCTGAAGC 
     1801 CCCCGGGGAC CCCACCGCCC TCCTCCCGAA AACTGATACA CTTGATCCCG GGATTCACCG 
     1861 CGCTGCATCG GAGCAAATCC CACGAGTTCC AGCTGGGGCA CCGCGTGGAC GAGGCCCACA 
     1921 CGCCCAAAGC CAAGAAGAAG AGCAAACCCT TGAACCTCAA GATCCACAGC AGCGTAGGCA 
     1981 GCTGCGAGAA CATCCCCTCT CAGCAGCGCT CCCCGCTGCT GTCCGAGCGC TCCCTCCGCT 
     2041 CCTTCTTTGT GGGACACGCA CCTTTCCTGC CTTCCACCCC TCCTGTTCAC ACTGAGGCCA 
     2101 ACTTCTCTGC AAACACACTG TCAGTGCCAC GCTGGTCCCC GCAGATCCCT CGCAGAGATC 
     2161 TCGGCAACTC CATCAAGCAC AGGTTTTCCA CCAAGTACTG GATGTCTCAG ACGTGCACAG 
     2221 TCTGTGGGAA AGGGATGCTT TTTGGCCTCA AGTGTAAAAA CTGCAAGTTA AAGTGCCACA 
     2281 ACAAATGCAC CAAAGAAGCC CCACCCTGTC ATCTTCTGAT CATCCACCGA GGAGATCCAG 
     2341 CAAGGTTAGT CCGGACAGAG TCCGTTCCGT GTGACATCAA CAACCCTCTA CGGAAGCCAC 
     2401 CTCGCTATTC AGACCTGCAC ATCAGTCAGA CGCTCCCCAA AACCAACAAA ATCAACAAGG 
     2461 ACCACATCCC TGTCCCTTAC CAGCCAGACT CCAGCAGCAA CCCCTCCTCC ACGACGTCCT 
     2521 CCACGCCCTC CTCGCCAGCA CCCCCCCTCC CTCCTAGTGC CACGCCGCCT TCTCCCCTAC 
     2581 ACCCTTCCCC ACAGTGCACA CGGCAGCAGA AGAACTTCAA CCTGCCAGCA TCCCACTACT 
     2641 ACAAATACAA GCAGCAGTTC ATCTTCCCAG ATGTGGTGCC GGTGCCGGAG ACGCCGACCC 
     2701 GGGCGCCCCA GGTCATCCTG CATCCGGTGA CCTCGAATCC AATCTTGGAA GGAAATCCAT 
     2761 TACTTCAAAT TGAAGTGGAG CCAACGTCGG AGAATGAAGA GGTCCATGAT GAGGCCGAAG 
     2821 AGTCAGAGGA TGACTTCGAG GAGATGAACC TGTCCCTCCT CTCGGCCCGG AGCTTCCCAC 
     2881 GCAAGGCCAG CCAGACCAGC ATCTTCCTTC AGGAGTGGGA CATCCCCTTT GAGCAGCTGG 
     2941 AGATCGGCGA GCTCATTGGA AAGGGCCGCT TTGGGCAAGT GTACCACGGC CGCTGGCATG 
     3001 GCGAGGTGGC CATCCGGCTG ATTGACATTG AGAGGGACAA CGAGGACCAG CTCAAGGCCT 
     3061 TCAAGCGGGA GGTGATGGCC TACAGGCAGA CACGGCATGA GAACGTGGTG CTTTTCATGG 
     3121 GTGCCTGCAT GAGCCCGCCT CACCTGGCCA TCATCACCAG CCTCTGTAAG GGACGGACGC 
     3181 TCTATTCCGT TGTGAGGGAT GCCAAAATCG TTTTGGATGT CAACAAAACC AGGCAGATTG 
     3241 CTCAAGAAAT TGTGAAGGGC ATGGGCTACC TCCACGCCAA GGGAATCCTA CACAAGGACC 
     3301 TCAAGTCAAA GAACGTCTTC TATGACAACG GCAAAGTGGT CATCACGGAC TTTGGACTCT 
     3361 TCAGCATTTC TGGGGTGCTG CAGGCTGGCA GGCGGGAGGA CAAACTGCGC ATCCAGAATG 
     3421 GCTGGCTATG CCACCTGGCA CCAGAGATCA TCCGCCAGCT GTCCCCCGAC ACAGAGGAGG 
     3481 ATAAGCTCCC CTTCTCCAAG CACTCTGACG TCTTTGCCCT TGGCACAATC TGGTATGAAC 
     3541 TCCACGCCAG GGAATGGCCT TTCAAGACCC AACCAGCAGA GGCAATAATC TGGCAAATGG 
     3601 GCACAGGCAT GAAACCCAAC CTCAGCCAGA TTGGCATGGG AAAAGAAATC TCGGACATTC 
     3661 TTCTCTTCTG CTGGGCCTTT GAACAAGAAG AGAGACCTAC CTTCACCAAG CTCATGGACA 
     3721 TGCTGGAGAA ACTGCCAAAG CGAAACCGTC GCCTGTCTCA CCCTGGACAT TTCTGGAAGT 
     3781 CTGCAGAGCT GACGCGTACG CGGCCGCTCG AGATGGAGAG CGACGAGAGC GGCCTGCCCG 
     3841 CCATGGAGAT CGAGTGCCGC ATCACCGGCA CCCTGAACGG CGTGGAGTTC GAGCTGGTGG 
     3901 GCGGCGGAGA GGGCACCCCC GAGCAGGGCC GCATGACCAA CAAGATGAAG AGCACCAAAG 
     3961 GCGCCCTGAC CTTCAGCCCC TACCTGCTGA GCCACGTGAT GGGCTACGGC TTCTACCACT 
     4021 TCGGCACCTA CCCCAGCGGC TACGAGAACC CCTTCCTGCA CGCCATCAAC AACGGCGGCT 
     4081 ACACCAACAC CCGCATCGAG AAGTACGAGG ACGGCGGCGT GCTGCACGTG AGCTTCAGCT 
     4141 ACCGCTACGA GGCCGGCCGC GTGATCGGCG ACTTCAAGGT GATGGGCACC GGCTTCCCCG 
     4201 AGGACAGCGT GATCTTCACC GACAAGATCA TCCGCAGCAA CGCCACCGTG GAGCACCTGC 
     4261 ACCCCATGGG CGATAACGAT CTGGATGGCA GCTTCACCCG CACCTTCAGC CTGCGCGACG 
     4321 GCGGCTACTA CAGCTCCGTG GTGGACAGCC ACATGCACTT CAAGAGCGCC ATCCACCCCA 
     4381 GCATCCTGCA GAACGGGGGC CCCATGTTCG CCTTCCGCCG CGTGGAGGAG GATCACAGCA 
     4441 ACACCGAGCT GGGCATCGTG GAGTACCAGC ACGCCTTCAA GACCCCGGAT GCAGATGCCG 
     4501 GTGAAGAAAG AGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4561 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4621 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4681 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4741 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4801 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4861 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4921 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4981 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5041 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5101 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5161 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCTTGGC TGTGGAATGT GTGTCAGTTA 
     5221 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 
     5281 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 
     5341 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 
     5401 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 
     5461 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 
     5521 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 
     5581 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 
     5641 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 
     5701 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 
     5761 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 
     5821 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 
     5881 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 
     5941 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 
     6001 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 
     6061 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 
     6121 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 
     6181 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 
     6241 GGTGTGGCCG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 
     6301 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 
     6361 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 
     6421 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 
     6481 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 
     6541 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 
     6601 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 
     6661 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 
     6721 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 
     6781 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 
     6841 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 
     6901 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 
     6961 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 
     7021 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 
     7081 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 
     7141 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 
     7201 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 
     7261 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 
     7321 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 
     7381 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 
     7441 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 
     7501 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 
     7561 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 
     7621 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 
     7681 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 
     7741 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 
     7801 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 
     7861 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 
     7921 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 
     7981 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 
     8041 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 
     8101 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 
     8161 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 
     8221 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 
     8281 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 
     8341 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 
     8401 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 
     8461 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 
     8521 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 
     8581 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 
     8641 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 
     8701 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 
     8761 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 
     8821 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 
     8881 AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 
     8941 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 
     9001 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG 
     9061 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 
     9121 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 
     9181 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 
     9241 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 
     9301 AACAAAAATT TAACGCGAAT TTT
//