LOCUS       RG211319                9209 bp    DNA     circular  SYN 
DEFINITION  ECT2 (tGFP-tagged) - Human epithelial cell transforming sequence 2 oncogene (ECT2)
ACCESSION   NM_018098
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3690..4394 
                     /label="GFP"  
     misc_feature    4441..5026 
                     /label="PolyA signal"  
     misc_feature    7118..7763 
                     /label="ColE1"  
     misc_feature    5480..6274 
                     /label="Neo"  
     misc_feature    5111..5413 
                     /label="SV40 ori"  
     misc_feature    7778..8638 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3669..3675 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3669 
                     /label="ECT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGAAAAT AGTGTATTAA CATCCACTAC TGGGAGGACT AGCTTGGCAG 
     1081 ACTCTTCCAT TTTTGATTCT AAAGTTACTG AGATTTCCAA GGAAAACTTA CTTATTGGAT 
     1141 CTACTTCATA TGTAGAAGAA GAGATGCCTC AGATTGAAAC AAGAGTGATA TTGGTTCAAG 
     1201 AAGCTGGAAA ACAAGAAGAA CTTATAAAAG CCTTAAAGGA CATTAAAGTG GGCTTTGTAA 
     1261 AGATGGAGTC AGTGGAAGAA TTTGAAGGTT TGGATTCTCC GGAATTTGAA AATGTATTTG 
     1321 TAGTCACGGA CTTTCAGGAT TCTGTCTTTA ATGACCTCTA CAAGGCTGAT TGTAGAGTTA 
     1381 TTGGACCACC AGTTGTATTA AATTGTTCAC AAAAAGGAGA GCCTTTGCCA TTTTCATGTC 
     1441 GCCCGTTGTA TTGTACAAGT ATGATGAATC TAGTACTATG CTTTACTGGA TTTAGGAAAA 
     1501 AAGAAGAACT AGTCAGGTTG GTGACATTGG TCCATCACAT GGGTGGAGTT ATTCGAAAAG 
     1561 ACTTTAATTC AAAAGTTACA CATTTGGTGG CAAATTGTAC ACAAGGAGAA AAATTCAGGG 
     1621 TTGCTGTGAG TCTAGGTACT CCAATTATGA AGCCAGAATG GATTTATAAA GCTTGGGAAA 
     1681 GGCGGAATGA ACAGGATTTC TATGCAGCAG TTGATGACTT TAGAAATGAA TTTAAAGTTC 
     1741 CTCCATTTCA AGATTGTATT TTAAGTTTCC TGGGATTTTC AGATGAAGAG AAAACCAATA 
     1801 TGGAAGAAAT GACTGAAATG CAAGGAGGTA AATATTTACC GCTTGGAGAT GAAAGATGCA 
     1861 CTCACCTTGT AGTTGAAGAG AATATAGTAA AAGATCTTCC CTTTGAACCT TCAAAGAAAC 
     1921 TTTATGTTGT CAAGCAAGAG TGGTTCTGGG GAAGCATTCA AATGGATGCC CGAGCTGGAG 
     1981 AAACTATGTA TTTATATGAA AAGGCAAATA CTCCTGAGCT CAAGAAATCA GTGTCAATGC 
     2041 TTTCTCTAAA TACCCCTAAC AGCAATCGCA AACGACGTCG TTTAAAAGAA ACACTTGCTC 
     2101 AGCTTTCAAG AGAGACAGAC GTGTCACCAT TTCCACCCCG TAAGCGCCCA TCAGCTGAGC 
     2161 ATTCCCTTTC CATAGGGTCA CTCCTAGATA TCTCCAACAC ACCAGAGTCT AGCATTAACT 
     2221 ATGGAGACAC CCCAAAGTCT TGTACTAAGT CTTCTAAAAG CTCCACTCCA GTTCCTTCAA 
     2281 AGCAGTCAGC AAGGTGGCAA GTTGCAAAAG AGCTTTATCA AACTGAAAGT AATTATGTTA 
     2341 ATATATTGGC AACAATTATT CAGTTATTTC AAGTACCATT GGAAGAGGAA GGACAACGTG 
     2401 GTGGACCTAT CCTTGCACCA GAGGAGATTA AGACTATTTT TGGTAGCATC CCAGATATCT 
     2461 TTGATGTACA CACTAAGATA AAGGATGATC TTGAAGACCT TATAGTTAAT TGGGATGAGA 
     2521 GCAAAAGCAT TGGTGACATT TTTCTGAAAT ATTCAAAAGA TTTGGTAAAA ACCTACCCTC 
     2581 CCTTTGTAAA CTTCTTTGAA ATGAGCAAGG AAACAATTAT TAAATGTGAA AAACAGAAAC 
     2641 CAAGATTTCA TGCTTTTCTC AAGATAAACC AAGCAAAACC AGAATGTGGA CGGCAGAGCC 
     2701 TTGTTGAACT TCTTATCCGA CCAGTACAGA GGTTACCCAG TGTTGCATTA CTTTTAAATG 
     2761 ATCTTAAGAA GCATACAGCT GATGAAAATC CAGACAAAAG CACTTTAGAA AAAGCTATTG 
     2821 GATCACTGAA GGAAGTAATG ACGCATATTA ATGAGGATAA GAGAAAAACA GAAGCTCAAA 
     2881 AGCAAATTTT TGATGTTGTT TATGAAGTAG ATGGATGCCC AGCTAATCTT TTATCTTCTC 
     2941 ACCGAAGCTT AGTACAGCGG GTTGAAACAA TTTCTCTAGG TGAGCACCCC TGTGACAGAG 
     3001 GAGAACAAGT AACTCTCTTC CTCTTCAATG ATTGCCTAGA GATAGCAAGA AAACGGCACA 
     3061 AGGTTATTGG CACTTTTAGG AGTCCTCATG GCCAAACCCG ACCCCCAGCT TCTCTTAAGC 
     3121 ATATTCACCT AATGCCTCTT TCTCAGATTA AGAAGGTATT GGACATAAGA GAGACAGAAG 
     3181 ATTGCCATAA TGCTTTTGCC TTGCTTGTGA GGCCACCAAC AGAGCAGGCA AATGTGCTAC 
     3241 TCAGTTTCCA GATGACATCA GATGAACTTC CAAAAGAAAA CTGGCTAAAG ATGCTGTGTC 
     3301 GACATGTAGC TAACACCATT TGTAAAGCAG ATGCTGAGAA TCTTATTTAT ACTGCTGATC 
     3361 CAGAATCCTT TGAAGTAAAT ACAAAAGATA TGGACAGTAC ATTGAGTAGA GCATCAAGAG 
     3421 CAATAAAAAA GACTTCAAAA AAGGTTACAA GAGCATTCTC TTTCTCCAAA ACTCCAAAAA 
     3481 GAGCTCTTCG AAGGGCTCTT ATGACATCCC ACGGCTCAGT GGAGGGAAGA AGTCCTTCCA 
     3541 GCAATGATAA GCATGTAATG AGTCGTCTTT CTAGCACATC ATCATTAGCA GGTATCCCTT 
     3601 CTCCCTCCCT TGTCAGCCTT CCTTCCTTCT TTGAAAGGAG AAGTCATACG TTAAGTAGAT 
     3661 CTACAACTCA TTTGATAACG CGTACGCGGC CGCTCGAGAT GGAGAGCGAC GAGAGCGGCC 
     3721 TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC 
     3781 TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA 
     3841 CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT 
     3901 ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG 
     3961 GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT 
     4021 TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT 
     4081 TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC 
     4141 ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC 
     4201 GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC 
     4261 ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC 
     4321 ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG 
     4381 ATGCCGGTGA AGAAAGAGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4441 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4501 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4561 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4621 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4681 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4741 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4801 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4861 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4921 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4981 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5041 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CTTGGCTGTG GAATGTGTGT 
     5101 CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 
     5161 CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG 
     5221 CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG 
     5281 CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT 
     5341 TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT 
     5401 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 
     5461 GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT 
     5521 TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC 
     5581 TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 
     5641 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG 
     5701 GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC 
     5761 TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC 
     5821 GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC 
     5881 TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 
     5941 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG 
     6001 TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT 
     6061 GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC 
     6121 CGGCTGGGTG TGGCCGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA 
     6181 GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 
     6241 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT 
     6301 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG 
     6361 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC 
     6421 AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA 
     6481 ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC 
     6541 ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA 
     6601 CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC 
     6661 CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT 
     6721 AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA 
     6781 ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA 
     6841 TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC 
     6901 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 
     6961 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 
     7021 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 
     7081 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 
     7141 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 
     7201 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 
     7261 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 
     7321 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 
     7381 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 
     7441 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 
     7501 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 
     7561 GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     7621 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     7681 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     7741 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA 
     7801 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 
     7861 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA 
     7921 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA 
     7981 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT 
     8041 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG 
     8101 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 
     8161 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG 
     8221 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG 
     8281 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT 
     8341 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT 
     8401 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 
     8461 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC 
     8521 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG 
     8581 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA 
     8641 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     8701 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     8761 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     8821 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     8881 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     8941 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     9001 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     9061 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     9121 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     9181 TGATTTAACA AAAATTTAAC GCGAATTTT
//