LOCUS RG211218 8060 bp DNA circular SYN DEFINITION TH (tGFP-tagged) - Human tyrosine hydroxylase (TH), transcript variant 2 ACCESSION NM_000360 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 2539..3243 /label="GFP" misc_feature 3290..3875 /label="PolyA signal" misc_feature 5967..6612 /label="ColE1" misc_feature 4329..5123 /label="Neo" misc_feature 3960..4262 /label="SV40 ori" misc_feature 6627..7487 /label="Amp" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 2511..2518 /label="Restriction Enzyme" misc_feature 1020..2511 /label="TH" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GCCCACCCCC GACGCCACCA CGCCACAGGC CAAGGGCTTC CGCAGGGCCG 1081 TGTCTGAGCT GGACGCCAAG CAGGCAGAGG CCATCATGTC CCCGCGGTTC ATTGGGCGCA 1141 GGCAGAGCCT CATCGAGGAC GCCCGCAAGG AGCGGGAGGC GGCGGTGGCA GCAGCGGCCG 1201 CTGCAGTCCC CTCGGAGCCC GGGGACCCCC TGGAGGCTGT GGCCTTTGAG GAGAAGGAGG 1261 GGAAGGCCAT GCTAAACCTG CTCTTCTCCC CGAGGGCCAC CAAGCCCTCG GCGCTGTCCC 1321 GAGCTGTGAA GGTGTTTGAG ACGTTTGAAG CCAAAATCCA CCATCTAGAG ACCCGGCCCG 1381 CCCAGAGGCC GCGAGCTGGG GGCCCCCACC TGGAGTACTT CGTGCGCCTC GAGGTGCGCC 1441 GAGGGGACCT GGCCGCCCTG CTCAGTGGTG TGCGCCAGGT GTCAGAGGAC GTGCGCAGCC 1501 CCGCGGGGCC CAAGGTCCCC TGGTTCCCAA GAAAAGTGTC AGAGCTGGAC AAGTGTCATC 1561 ACCTGGTCAC CAAGTTCGAC CCTGACCTGG ACTTGGACCA CCCGGGCTTC TCGGACCAGG 1621 TGTACCGCCA GCGCAGGAAG CTGATTGCTG AGATCGCCTT CCAGTACAGG CACGGCGACC 1681 CGATTCCCCG TGTGGAGTAC ACCGCCGAGG AGATTGCCAC CTGGAAGGAG GTCTACACCA 1741 CGCTGAAGGG CCTCTACGCC ACGCACGCCT GCGGGGAGCA CCTGGAGGCC TTTGCTTTGC 1801 TGGAGCGCTT CAGCGGCTAC CGGGAAGACA ATATCCCCCA GCTGGAGGAC GTCTCCCGCT 1861 TCCTGAAGGA GCGCACGGGC TTCCAGCTGC GGCCTGTGGC CGGCCTGCTG TCCGCCCGGG 1921 ACTTCCTGGC CAGCCTGGCC TTCCGCGTGT TCCAGTGCAC CCAGTATATC CGCCACGCGT 1981 CCTCGCCCAT GCACTCCCCT GAGCCGGACT GCTGCCACGA GCTGCTGGGG CACGTGCCCA 2041 TGCTGGCCGA CCGCACCTTC GCGCAGTTCT CGCAGGACAT TGGCCTGGCG TCCCTGGGGG 2101 CCTCGGATGA GGAAATTGAG AAGCTGTCCA CGCTGTACTG GTTCACGGTG GAGTTCGGGC 2161 TGTGTAAGCA GAACGGGGAG GTGAAGGCCT ATGGTGCCGG GCTGCTGTCC TCCTACGGGG 2221 AGCTCCTGCA CTGCCTGTCT GAGGAGCCTG AGATTCGGGC CTTCGACCCT GAGGCTGCGG 2281 CCGTGCAGCC CTACCAAGAC CAGACGTACC AGTCAGTCTA CTTCGTGTCT GAGAGCTTCA 2341 GTGACGCCAA GGACAAGCTC AGGAGCTATG CCTCACGCAT CCAGCGCCCC TTCTCCGTGA 2401 AGTTCGACCC GTACACGCTG GCCATCGACG TGCTGGACAG CCCCCAGGCC GTGCGGCGCT 2461 CCCTGGAGGG TGTCCAGGAT GAGCTGGACA CCCTTGCCCA TGCGCTGAGT GCCATTGGCA 2521 GCGGACCGAC GCGTACGCGG CCGCTCGAGA TGGAGAGCGA CGAGAGCGGC CTGCCCGCCA 2581 TGGAGATCGA GTGCCGCATC ACCGGCACCC TGAACGGCGT GGAGTTCGAG CTGGTGGGCG 2641 GCGGAGAGGG CACCCCCGAG CAGGGCCGCA TGACCAACAA GATGAAGAGC ACCAAAGGCG 2701 CCCTGACCTT CAGCCCCTAC CTGCTGAGCC ACGTGATGGG CTACGGCTTC TACCACTTCG 2761 GCACCTACCC CAGCGGCTAC GAGAACCCCT TCCTGCACGC CATCAACAAC GGCGGCTACA 2821 CCAACACCCG CATCGAGAAG TACGAGGACG GCGGCGTGCT GCACGTGAGC TTCAGCTACC 2881 GCTACGAGGC CGGCCGCGTG ATCGGCGACT TCAAGGTGAT GGGCACCGGC TTCCCCGAGG 2941 ACAGCGTGAT CTTCACCGAC AAGATCATCC GCAGCAACGC CACCGTGGAG CACCTGCACC 3001 CCATGGGCGA TAACGATCTG GATGGCAGCT TCACCCGCAC CTTCAGCCTG CGCGACGGCG 3061 GCTACTACAG CTCCGTGGTG GACAGCCACA TGCACTTCAA GAGCGCCATC CACCCCAGCA 3121 TCCTGCAGAA CGGGGGCCCC ATGTTCGCCT TCCGCCGCGT GGAGGAGGAT CACAGCAACA 3181 CCGAGCTGGG CATCGTGGAG TACCAGCACG CCTTCAAGAC CCCGGATGCA GATGCCGGTG 3241 AAGAAAGAGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 3301 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 3361 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 3421 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 3481 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 3541 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 3601 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 3661 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 3721 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 3781 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 3841 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 3901 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCTTGGCTGT GGAATGTGTG TCAGTTAGGG 3961 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 4021 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 4081 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 4141 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 4201 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 4261 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 4321 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 4381 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 4441 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 4501 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 4561 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 4621 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 4681 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 4741 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 4801 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 4861 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 4921 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 4981 GTGGCCGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 5041 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 5101 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA 5161 CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG 5221 AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG 5281 ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA 5341 AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT 5401 GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT 5461 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA 5521 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 5581 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT 5641 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT 5701 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 5761 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 5821 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 5881 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 5941 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 6001 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 6061 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 6121 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 6181 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 6241 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 6301 CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 6361 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTT 6421 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA 6481 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 6541 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT 6601 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG 6661 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT 6721 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG 6781 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 6841 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 6901 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 6961 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 7021 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 7081 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 7141 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 7201 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 7261 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 7321 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 7381 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 7441 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 7501 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 7561 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 7621 TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA 7681 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT 7741 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT 7801 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC 7861 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT 7921 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT 7981 TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC 8041 AAAAATTTAA CGCGAATTTT //