LOCUS       RG209677                7561 bp    DNA     circular  SYN 
DEFINITION  NPBWR2 (tGFP-tagged) - Human neuropeptides B/W receptor 2 (NPBWR2)
ACCESSION   NM_005286
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2042..2746 
                     /label="GFP"  
     misc_feature    2793..3378 
                     /label="PolyA signal"  
     misc_feature    5470..6115 
                     /label="ColE1"  
     misc_feature    3832..4626 
                     /label="Neo"  
     misc_feature    3463..3765 
                     /label="SV40 ori"  
     misc_feature    6130..6990 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2021..2027 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2021 
                     /label="NPBWR2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGCCGCT GGGCACCCAG AGCCCCTTGA CAGCAGGGGC TCCTTCTCCC 
     1081 TCCCCACGAT GGGTGCCAAC GTCTCTCAGG ACAATGGCAC TGGCCACAAT GCCACCTTCT 
     1141 CCGAGCCACT GCCGTTCCTC TATGTGCTCC TGCCCGCCGT GTACTCCGGG ATCTGTGCTG 
     1201 TGGGGCTGAC TGGCAACACG GCCGTCATCC TTGTAATCCT AAGGGCGCCC AAGATGAAGA 
     1261 CGGTGACCAA CGTGTTCATC CTGAACCTGG CCGTCGCCGA CGGGCTCTTC ACGCTGGTAC 
     1321 TGCCTGTCAA CATCGCGGAG CACCTGCTGC AGTACTGGCC CTTCGGGGAG CTGCTCTGCA 
     1381 AGCTGGTGCT GGCCGTCGAC CACTACAACA TCTTCTCCAG CATCTACTTC CTAGCCGTGA 
     1441 TGAGCGTGGA CCGATACCTG GTGGTGCTGG CCACCGTGAG GTCCCGCCAC ATGCCCTGGC 
     1501 GCACCTACCG GGGGGCGAAG GTCGCCAGCC TGTGTGTCTG GCTGGGCGTC ACGGTCCTGG 
     1561 TTCTGCCCTT CTTCTCTTTC GCTGGCGTCT ACAGCAACGA GCTGCAGGTC CCAAGCTGTG 
     1621 GGCTGAGCTT CCCGTGGCCC GAGCAGGTCT GGTTCAAGGC CAGCCGTGTC TACACGTTGG 
     1681 TCCTGGGCTT CGTGCTGCCC GTGTGCACCA TCTGTGTGCT CTACACAGAC CTCCTGCGCA 
     1741 GGCTGCGGGC CGTGCGGCTC CGCTCTGGAG CCAAGGCTCT AGGCAAGGCC AGGCGGAAGG 
     1801 TGACCGTCCT GGTCCTCGTC GTGCTGGCCG TGTGCCTCCT CTGCTGGACG CCCTTCCACC 
     1861 TGGCCTCTGT CGTGGCCCTG ACCACGGACC TGCCCCAGAC CCCACTGGTC ATCAGTATGT 
     1921 CCTACGTCAT CACCAGCCTC AGCTACGCCA ACTCGTGCCT GAACCCCTTC CTCTACGCCT 
     1981 TTCTAGATGA CAACTTCCGG AAGAACTTCC GCAGCATATT GCGGTGCTGA CGCGTACGCG 
     2041 GCCGCTCGAG ATGGAGAGCG ACGAGAGCGG CCTGCCCGCC ATGGAGATCG AGTGCCGCAT 
     2101 CACCGGCACC CTGAACGGCG TGGAGTTCGA GCTGGTGGGC GGCGGAGAGG GCACCCCCGA 
     2161 GCAGGGCCGC ATGACCAACA AGATGAAGAG CACCAAAGGC GCCCTGACCT TCAGCCCCTA 
     2221 CCTGCTGAGC CACGTGATGG GCTACGGCTT CTACCACTTC GGCACCTACC CCAGCGGCTA 
     2281 CGAGAACCCC TTCCTGCACG CCATCAACAA CGGCGGCTAC ACCAACACCC GCATCGAGAA 
     2341 GTACGAGGAC GGCGGCGTGC TGCACGTGAG CTTCAGCTAC CGCTACGAGG CCGGCCGCGT 
     2401 GATCGGCGAC TTCAAGGTGA TGGGCACCGG CTTCCCCGAG GACAGCGTGA TCTTCACCGA 
     2461 CAAGATCATC CGCAGCAACG CCACCGTGGA GCACCTGCAC CCCATGGGCG ATAACGATCT 
     2521 GGATGGCAGC TTCACCCGCA CCTTCAGCCT GCGCGACGGC GGCTACTACA GCTCCGTGGT 
     2581 GGACAGCCAC ATGCACTTCA AGAGCGCCAT CCACCCCAGC ATCCTGCAGA ACGGGGGCCC 
     2641 CATGTTCGCC TTCCGCCGCG TGGAGGAGGA TCACAGCAAC ACCGAGCTGG GCATCGTGGA 
     2701 GTACCAGCAC GCCTTCAAGA CCCCGGATGC AGATGCCGGT GAAGAAAGAG TTTAAACGGC 
     2761 CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC 
     2821 CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA 
     2881 AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG 
     2941 GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT 
     3001 GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG 
     3061 TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA 
     3121 GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG 
     3181 TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC 
     3241 AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG 
     3301 AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT 
     3361 GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG 
     3421 GTACGCGGCC AGCTTGGCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT 
     3481 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA 
     3541 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 
     3601 CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT 
     3661 CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT 
     3721 CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC 
     3781 TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC 
     3841 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 
     3901 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT 
     3961 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 
     4021 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG 
     4081 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 
     4141 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 
     4201 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC 
     4261 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 
     4321 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG 
     4381 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 
     4441 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCCGAC CGCTATCAGG 
     4501 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT 
     4561 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC 
     4621 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA 
     4681 CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT 
     4741 CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT 
     4801 CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 
     4861 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 
     4921 CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG 
     4981 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 
     5041 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 
     5101 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 
     5161 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 
     5221 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5281 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5341 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5401 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5461 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5521 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5581 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5641 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5701 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5761 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5821 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5881 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA 
     5941 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6001 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6061 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6121 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 
     6181 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 
     6241 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 
     6301 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 
     6361 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 
     6421 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 
     6481 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 
     6541 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 
     6601 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 
     6661 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 
     6721 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 
     6781 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 
     6841 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 
     6901 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 
     6961 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 
     7021 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 
     7081 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG 
     7141 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 
     7201 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 
     7261 CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 
     7321 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 
     7381 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 
     7441 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 
     7501 GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT 
     7561 T
//