LOCUS       RG208364                9554 bp    DNA     circular  SYN 
DEFINITION  EPHB3 (GFP-tagged) - Human EPH receptor B3 (EPHB3)
ACCESSION   NM_004443
VERSION     NM_004443.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4035..4739 
                     /label="GFP"  
     misc_feature    4786..5371 
                     /label="PolyA signal"  
     misc_feature    7463..8108 
                     /label="ColE1"  
     misc_feature    5825..6619 
                     /label="Neo"  
     misc_feature    5456..5758 
                     /label="SV40 ori"  
     misc_feature    8123..8983 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4014..4020 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4014 
                     /label="EPHB3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGAGCC CGCCCGCCGC CGCCGCCGTC GCCGCCGCCG GGGCTTCTGC 
     1081 CGCTGCTCCC TCCGCTGCTG CTGCTGCCGC TGCTGCTGCT GCCCGCCGGC TGCCGGGCGC 
     1141 TGGAAGAGAC CCTCATGGAC ACAAAATGGG TAACATCTGA GTTGGCGTGG ACATCTCATC 
     1201 CAGAAAGTGG GTGGGAAGAG GTGAGTGGCT ACGATGAGGC CATGAATCCC ATCCGCACAT 
     1261 ACCAGGTGTG TAATGTGCGC GAGTCAAGCC AGAACAACTG GCTTCGCACG GGGTTCATCT 
     1321 GGCGGCGGGA TGTGCAGCGG GTCTACGTGG AGCTCAAGTT CACTGTGCGT GACTGCAACA 
     1381 GCATCCCCAA CATCCCCGGC TCCTGCAAGG AGACCTTCAA CCTCTTCTAC TACGAGGCTG 
     1441 ACAGCGATGT GGCCTCAGCC TCCTCCCCCT TCTGGATGGA GAACCCCTAC GTGAAAGTGG 
     1501 ACACCATTGC ACCCGATGAG AGCTTCTCGC GGCTGGATGC CGGCCGTGTC AACACCAAGG 
     1561 TGCGCAGCTT TGGGCCACTT TCCAAGGCTG GCTTCTACCT GGCCTTCCAG GACCAGGGCG 
     1621 CCTGCATGTC GCTCATCTCC GTGCGCGCCT TCTACAAGAA GTGTGCATCC ACCACCGCAG 
     1681 GCTTCGCACT CTTCCCCGAG ACCCTCACTG GGGCGGAGCC CACCTCGCTG GTCATTGCTC 
     1741 CTGGCACCTG CATCCCTAAC GCCGTGGAGG TGTCGGTGCC ACTCAAGCTC TACTGCAACG 
     1801 GCGATGGGGA GTGGATGGTG CCTGTGGGTG CCTGCACCTG TGCCACCGGC CATGAGCCAG 
     1861 CTGCCAAGGA GTCCCAGTGC CGCCCCTGTC CCCCTGGGAG CTACAAGGCG AAGCAGGGAG 
     1921 AGGGGCCCTG CCTCCCATGT CCCCCCAACA GCCGTACCAC CTCCCCAGCC GCCAGCATCT 
     1981 GCACCTGCCA CAATAACTTC TACCGTGCAG ACTCGGACTC TGCGGACAGT GCCTGTACCA 
     2041 CCGTGCCATC TCCACCCCGA GGTGTGATCT CCAATGTGAA TGAAACCTCA CTGATCCTCG 
     2101 AGTGGAGTGA GCCCCGGGAC CTGGGTGGCC GGGATGACCT CCTGTACAAT GTCATCTGCA 
     2161 AGAAGTGCCA TGGGGCTGGA GGGGCCTCAG CCTGCTCACG CTGTGATGAC AACGTGGAGT 
     2221 TTGTGCCTCG GCAGCTGGGC CTGACGGAGC GCCGGGTCCA CATCAGCCAT CTGCTGGCCC 
     2281 ACACGCGCTA CACCTTTGAG GTGCAGGCGG TCAACGGTGT CTCGGGCAAG AGCCCTCTGC 
     2341 CGCCTCGTTA TGCGGCCGTG AATATCACCA CAAACCAGGC TGCCCCGTCT GAAGTGCCCA 
     2401 CACTACGCCT GCACAGCAGC TCAGGCAGCA GCCTCACCCT ATCCTGGGCA CCCCCAGAGC 
     2461 GGCCCAACGG AGTCATCCTG GACTACGAGA TGAAGTACTT TGAGAAGAGC GAGGGCATCG 
     2521 CCTCCACAGT GACCAGCCAG ATGAACTCCG TGCAGCTGGA CGGGCTTCGG CCTGACGCCC 
     2581 GCTATGTGGT CCAGGTCCGT GCCCGCACAG TAGCTGGCTA TGGGCAGTAC AGCCGCCCTG 
     2641 CCGAGTTTGA GACCACAAGT GAGAGAGGCT CTGGGGCCCA GCAGCTCCAG GAGCAGCTTC 
     2701 CCCTCATCGT GGGCTCCGCT ACAGCTGGGC TTGTCTTCGT GGTGGCTGTC GTGGTCATCG 
     2761 CTATCGTCTG CCTCAGGAAG CAGCGACACG GCTCTGATTC GGAGTACACG GAGAAGCTGC 
     2821 AGCAGTACAT TGCTCCTGGA ATGAAGGTTT ATATTGACCC TTTTACCTAC GAGGACCCTA 
     2881 ATGAGGCTGT TCGGGAGTTT GCCAAGGAGA TCGACGTGTC CTGCGTCAAG ATCGAGGAGG 
     2941 TGATCGGAGC TGGGGAATTT GGGGAAGTGT GCCGTGGTCG ACTGAAACAG CCTGGCCGCC 
     3001 GAGAGGTGTT TGTGGCCATC AAGACGCTGA AGGTGGGCTA CACCGAGAGG CAGCGGCGGG 
     3061 ACTTCCTAAG CGAGGCCTCC ATCATGGGTC AGTTTGATCA CCCCAATATA ATCCGGCTCG 
     3121 AGGGCGTGGT CACCAAAAGT CGGCCAGTTA TGATCCTCAC TGAGTTCATG GAAAACTGCG 
     3181 CCCTGGACTC CTTCCTCCGG CTCAACGATG GGCAGTTCAC GGTCATCCAG CTGGTGGGCA 
     3241 TGTTGCGGGG CATTGCTGCC GGCATGAAGT ACCTGTCCGA GATGAACTAT GTGCACCGCG 
     3301 ACCTGGCTGC TCGCAACATC CTTGTCAACA GCAACCTGGT CTGCAAAGTC TCAGACTTTG 
     3361 GCCTCTCCCG CTTCCTGGAG GATGACCCCT CCGATCCTAC CTACACCAGT TCCCTGGGCG 
     3421 GGAAGATCCC CATCCGCTGG ACTGCCCCAG AGGCCATAGC CTATCGGAAG TTCACTTCTG 
     3481 CTAGTGATGT CTGGAGCTAC GGAATTGTCA TGTGGGAGGT CATGAGCTAT GGAGAGCGAC 
     3541 CCTACTGGGA CATGAGCAAC CAGGATGTCA TCAATGCCGT GGAGCAGGAT TACCGGCTGC 
     3601 CACCACCCAT GGACTGTCCC ACAGCACTGC ACCAGCTCAT GCTGGACTGC TGGGTGCGGG 
     3661 ACCGGAACCT CAGGCCCAAA TTCTCCCAGA TTGTCAATAC CCTGGACAAG CTCATCCGCA 
     3721 ATGCTGCCAG CCTCAAGGTC ATTGCCAGCG CTCAGTCTGG CATGTCACAG CCCCTCCTGG 
     3781 ACCGCACGGT CCCAGATTAC ACAACCTTCA CGACAGTTGG TGATTGGCTG GATGCCATCA 
     3841 AGATGGGGCG GTACAAGGAG AGCTTCGTCA GTGCGGGGTT TGCATCTTTT GACCTGGTGG 
     3901 CCCAGATGAC GGCAGAAGAC CTGCTCCGTA TTGGGGTCAC CCTGGCCGGC CACCAGAAGA 
     3961 AGATCCTGAG CAGTATCCAG GACATGCGGC TGCAGATGAA CCAGACGCTG CCTGTGCAGG 
     4021 TCACGCGTAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     4081 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     4141 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     4201 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     4261 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4321 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4381 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4441 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4501 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4561 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4621 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4681 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4741 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4801 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4861 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4921 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4981 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5041 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5101 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5161 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5221 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5281 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5341 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5401 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5461 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5521 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5581 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5641 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5701 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5761 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5821 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5881 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5941 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     6001 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     6061 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     6121 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     6181 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     6241 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6301 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6361 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6421 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6481 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6541 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6601 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6661 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6721 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6781 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6841 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6901 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6961 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     7021 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     7081 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     7141 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     7201 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     7261 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7321 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7381 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7441 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7501 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7561 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7621 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7681 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7741 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7801 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7861 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7921 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7981 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     8041 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     8101 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     8161 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     8221 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     8281 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8341 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8401 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8461 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8521 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8581 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8641 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8701 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8761 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8821 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8881 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8941 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     9001 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     9061 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     9121 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     9181 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     9241 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9301 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9361 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9421 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9481 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9541 TTAACGCGAA TTTT
//