LOCUS       RG208361                9494 bp    DNA     circular  SYN 
DEFINITION  PEX6 (tGFP-tagged) - Human peroxisomal biogenesis factor 6 (PEX6)
ACCESSION   NM_000287
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3973..4677 
                     /label="GFP"  
     misc_feature    4724..5309 
                     /label="PolyA signal"  
     misc_feature    7401..8046 
                     /label="ColE1"  
     misc_feature    5763..6557 
                     /label="Neo"  
     misc_feature    5394..5696 
                     /label="SV40 ori"  
     misc_feature    8061..8921 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3960..3968 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3960 
                     /label="PEX6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGGCT GTCTTGCGGG TCCTGGAGCC CTTTCCGACC GAGACACCCC 
     1081 CGTTGGCAGT GCTGCTGCCA CCCGGGGGCC CGTGGCCGGC GGCGGAGCTG GGCCTGGTGC 
     1141 TGGCCCTGAG GCCTGCAGGG GAGAGCCCGG CAGGGCCGGC GCTGCTGGTG GCAGCCCTGG 
     1201 AGGGGCCGGA CGCGGGCACC GAAGAGCAGG GTCCCGGGCC GCCGCAGCTA CTGGTTAGCC 
     1261 GCGCGCTGCT GCGGCTCCTG GCACTGGGCT CCGGGGCCTG GGTGCGGGCG CGGGCGGTGC 
     1321 GGCGGCCCCC GGCGCTAGGT TGGGCACTGC TTGGCACCTC GCTGGGGCCT GGGCTCGGAC 
     1381 CGCGAGTCGG GCCGCTGCTG GTGAGGCGCG GAGAGACCCT CCCAGTGCCC GGACCGCGGG 
     1441 TGCTGGAGAC GCGGCCGGCG TTGCAAGGGC TGCTGGGCCC AGGGACTCGG CTGGCTGTGA 
     1501 CTGAGCTCCG CGGGCGGGCC AGACTGTGTC CAGAGTCTGG GGACAGCAGT CGGCCCCCAC 
     1561 CCCCGCCCGT GGTGTCCTCC TTTGCGGTTT CTGGCACAGT GCGGCGACTC CAGGGAGTTC 
     1621 TGGGAGGGAC TGGAGATTCA CTAGGGGTGA GCCGGAGCTG TCTCCGTGGC CTTGGCCTCT 
     1681 TCCAGGGCGA ATGGGTGTGG GTGGCCCAGG CCAGAGAGTC ATCGAACACT TCACAGCCGC 
     1741 ACTTGGCTAG GGTGCAGGTC CTAGAACCTC GCTGGGACCT CTCTGATAGA CTGGGACCCG 
     1801 GCTCTGGACC GCTGGGAGAG CCCCTCGCTG ACGGACTGGC GCTTGTCCCT GCCACTTTGG 
     1861 CTTTTAATCT TGGCTGTGAC CCCCTGGAAA TGGGAGAGCT CAGAATTCAG AGGTACTTGG 
     1921 AAGGCTCCAT CGCCCCTGAA GACAAAGGAA GCTGCTCATT GCTGCCTGGG CCTCCATTTG 
     1981 CCAGAGAGTT ACACATCGAA ATTGTGTCTT CTCCCCACTA CAGCACTAAT GGAAATTATG 
     2041 ACGGTGTTCT TTACCGGCAC TTTCAGATAC CCAGGGTAGT CCAGGAAGGG GATGTTCTAT 
     2101 GTGTGCCAAC AATTGGGCAA GTAGAGATCC TGGAAGGAAG TCCAGAGAAA CTGCCCAGGT 
     2161 GGCGGGAAAT GTTTTTTAAA GTGAAGAAAA CAGTTGGGGA AGCTCCAGAT GGACCAGCCA 
     2221 GTGCCTACTT GGCCGACACC ACCCATACCT CCTTGTACAT GGTGGGTTCT ACCCTGAGCC 
     2281 CTGTTCCATG GCTCCCTTCA GAGGAATCCA CTCTCTGGAG CAGTTTGTCT CCTCCAGGCC 
     2341 TGGAGGCCTT GGTGTCTGAA CTCTGTGCTG TCCTGAAGCC TCGCCTCCAG CCAGGGGGTG 
     2401 CCCTGCTGAC AGGAACTAGC AGTGTCCTTC TACGGGGCCC CCCAGGCTGT GGGAAGACCA 
     2461 CAGTAGTTGC TGCTGCCTGT AGTCACCTTG GGCTCCACTT ACTGAAGGTG CCCTGCTCCA 
     2521 GCCTCTGTGC AGAAAGTAGT GGGGCTGTGG AGACAAAACT GCAGGCCATC TTCTCCCGGG 
     2581 CCCGCCGTTG CCGGCCTGCA GTCCTGTTGC TCACAGCTGT GGACCTTCTG GGCCGGGACC 
     2641 GTGATGGGCT GGGTGAGGAT GCCCGTGTGA TGGCTGTGCT GCGTCACCTC CTCCTCAATG 
     2701 AGGACCCCCT CAACAGCTGC CCTCCCCTCA TGGTTGTGGC CACCACAAGC CGGGCCCAGG 
     2761 ACCTGCCTGC TGATGTGCAG ACAGCATTTC CTCATGAGCT CGAGGTGCCT GCTCTGTCAG 
     2821 AGGGGCAGCG GCTCAGCATC CTGCGGGCCC TCACTGCCCA CCTTCCCCTG GGCCAGGAGG 
     2881 TGAACTTGGC ACAGCTAGCA CGGCGGTGTG CAGGCTTTGT GGTAGGGGAT CTCTATGCCC 
     2941 TTCTGACCCA CAGCAGCCGG GCAGCCTGCA CCAGGATCAA GAACTCAGGT TTGGCAGGTG 
     3001 GCTTGACTGA GGAGGATGAG GGGGAGCTGT GTGCTGCCGG CTTTCCTCTC CTGGCTGAGG 
     3061 ACTTTGGGCA GGCACTGGAG CAACTGCAGA CAGCTCACTC CCAGGCCGTT GGAGCCCCCA 
     3121 AGATCCCCTC AGTGTCCTGG CATGATGTGG GTGGGCTGCA GGAGGTGAAG AAGGAGATCC 
     3181 TGGAGACCAT TCAGCTCCCC CTGGAGCACC CTGAGCTACT GAGCCTGGGC CTGAGACGCT 
     3241 CAGGCCTTCT GCTCCATGGG CCCCCTGGCA CCGGCAAGAC CCTTCTGGCC AAGGCAGTAG 
     3301 CCACTGAGTG CAGCCTTACC TTCCTCAGCG TGAAGGGGCC AGAGCTCATT AACATGTATG 
     3361 TGGGCCAAAG TGAGGAGAAT GTGCGGGAAG TATTTGCCAG GGCCAGGGCT GCAGCTCCAT 
     3421 GCATTATCTT CTTTGATGAA CTGGACTCTT TGGCCCCAAG CCGGGGGCGA AGTGGAGATT 
     3481 CTGGAGGAGT GATGGACAGG GTGGTGTCTC AGCTCCTTGC CGAGCTAGAT GGGCTGCACA 
     3541 GCACTCAGGA TGTGTTTGTG ATTGGAGCCA CCAACAGACC AGATCTCCTG GACCCTGCCC 
     3601 TTCTGCGGCC TGGCAGATTT GACAAGCTGG TGTTTGTGGG GGCAAATGAG GACCGGGCCT 
     3661 CCCAGCTACG CGTTCTAAGT GCCATCACAC GCAAATTCAA GCTAGAGCCA TCTGTGAGCC 
     3721 TGGTAAACGT GCTAGATTGC TGCCCTCCCC AGCTGACGGG CGCGGACCTC TACTCTCTCT 
     3781 GCTCTGATGC TATGACAGCT GCCCTCAAAC GCAGGGTTCA TGACCTGGAG GAAGGGCTGG 
     3841 AGCCAGGTAG CTCAGCACTG ATGCTCACCA TGGAGGACTT GCTGCAGGCT GCCGCCCGGC 
     3901 TGCAACCCTC AGTCAGTGAG CAGGAGCTGC TCCGGTACAA GCGCATCCAG CGCAAGTTTG 
     3961 CTGCCTGCAC GCGGCCGCTC GAGATGGAGA GCGACGAGAG CGGCCTGCCC GCCATGGAGA 
     4021 TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG 
     4081 AGGGCACCCC CGAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA GGCGCCCTGA 
     4141 CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC TTCGGCACCT 
     4201 ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC TACACCAACA 
     4261 CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC TACCGCTACG 
     4321 AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGATGGGCAC CGGCTTCCCC GAGGACAGCG 
     4381 TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG CACCCCATGG 
     4441 GCGATAACGA TCTGGATGGC AGCTTCACCC GCACCTTCAG CCTGCGCGAC GGCGGCTACT 
     4501 ACAGCTCCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC AGCATCCTGC 
     4561 AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GGATCACAGC AACACCGAGC 
     4621 TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCGGA TGCAGATGCC GGTGAAGAAA 
     4681 GAGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCTTGG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 
     5401 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 
     5461 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 
     5521 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 
     5581 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 
     5641 GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 
     5701 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 
     5761 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 
     5821 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 
     5881 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 
     5941 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 
     6001 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 
     6061 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 
     6121 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 
     6181 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 
     6241 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 
     6301 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 
     6361 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCC 
     6421 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 
     6481 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 
     6541 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 
     6601 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 
     6661 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 
     6721 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 
     6781 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 
     6841 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 
     6901 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 
     6961 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 
     7021 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 
     7081 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 
     7141 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
     7201 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
     7261 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
     7321 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
     7381 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
     7441 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
     7501 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
     7561 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
     7621 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
     7681 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
     7741 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
     7801 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTTTTTTTG 
     7861 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 
     7921 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 
     7981 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 
     8041 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 
     8101 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 
     8161 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 
     8221 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 
     8281 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 
     8341 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 
     8401 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 
     8461 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 
     8521 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 
     8581 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 
     8641 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 
     8701 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 
     8761 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 
     8821 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 
     8881 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 
     8941 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 
     9001 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 
     9061 CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA 
     9121 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG 
     9181 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT 
     9241 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG 
     9301 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA 
     9361 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT 
     9421 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT 
     9481 TTAACGCGAA TTTT
//