LOCUS       RG208263                8828 bp    DNA     circular  SYN 
DEFINITION  PLCD1 (tGFP-tagged) - Human phospholipase C, delta 1 (PLCD1), transcript variant 2
ACCESSION   NM_006225
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3309..4013 
                     /label="GFP"  
     misc_feature    4060..4645 
                     /label="PolyA signal"  
     misc_feature    6737..7382 
                     /label="ColE1"  
     misc_feature    5099..5893 
                     /label="Neo"  
     misc_feature    4730..5032 
                     /label="SV40 ori"  
     misc_feature    7397..8257 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3288..3294 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3288 
                     /label="PLCD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACTCGGGC CGGGACTTCC TGACCCTGCA CGGCCTACAG GATGATGAGG 
     1081 ATCTACAGGC GCTGCTGAAG GGCAGCCAGC TCCTGAAGGT GAAGTCCAGC TCATGGAGGA 
     1141 GAGAGCGCTT CTACAAGTTG CAGGAGGACT GCAAGACCAT CTGGCAGGAG TCCCGCAAGG 
     1201 TCATGCGGAC CCCGGAGTCC CAGCTGTTCT CCATCGAGGA CATTCAGGAG GTGCGAATGG 
     1261 GGCACCGCAC GGAGGGTCTG GAGAAGTTCG CCCGTGATGT GCCCGAGGAC CGCTGCTTCT 
     1321 CCATTGTCTT CAAGGACCAG CGCAATACAC TAGACCTCAT CGCCCCATCG CCAGCTGATG 
     1381 CCCAGCACTG GGTGCTGGGG CTGCACAAGA TCATCCACCA CTCAGGCTCC ATGGACCAGC 
     1441 GTCAGAAGCT ACAGCACTGG ATTCACTCCT GCTTGCGAAA AGCTGACAAA AACAAGGACA 
     1501 ACAAGATGAG CTTCAAGGAG CTGCAGAACT TCCTGAAGGA GCTCAACATC CAGGTGGACG 
     1561 ACAGCTATGC CCGGAAGATC TTCAGGGAGT GTGACCACTC CCAGACAGAC TCCCTGGAGG 
     1621 ACGAGGAGAT TGAGGCCTTC TACAAGATGC TGACCCAGCG GGTGGAGATC GACCGCACCT 
     1681 TCGCCGAGGC CGCGGGCTCA GGGGAGACTC TGTCGGTGGA TCAGTTAGTG ACGTTCCTGC 
     1741 AGCACCAGCA GCGGGAGGAG GCGGCAGGGC CTGCGCTGGC CCTCTCCCTC ATTGAGCGCT 
     1801 ACGAGCCCAG CGAGACTGCC AAGGCGCAGC GGCAGATGAC CAAGGACGGC TTCCTCATGT 
     1861 ACTTACTGTC GGCTGACGGC AGCGCCTTCA GCCTGGCACA CCGCCGTGTC TACCAGGACA 
     1921 TGGGCCAGCC ACTTAGCCAC TACCTGGTGT CCTCTTCACA CAACACCTAC CTGCTGGAGG 
     1981 ACCAGCTAGC CGGGCCCAGC AGCACTGAAG CCTACATCCG GGCACTGTGC AAAGGCTGCC 
     2041 GATGCCTGGA GCTTGACTGC TGGGACGGGC CCAACCAGGA ACCAATCATC TACCACGGCT 
     2101 ATACTTTCAC TTCCAAGATC CTCTTCTGCG ATGTGCTCAG GGCCATCCGG GACTATGCCT 
     2161 TCAAGGCGTC CCCCTACCCT GTCATCCTAT CCCTGGAGAA CCACTGCACA CTGGAGCAGC 
     2221 AGCGCGTGAT GGCGCGGCAC CTGCATGCCA TCCTGGGCCC CATGCTGTTG AACCGACCAC 
     2281 TGGATGGGGT CACCAACAGC CTGCCCTCCC CTGAGCAACT GAAGGGGAAG ATCCTGCTGA 
     2341 AGGGGAAGAA GCTCGGGGGG CTCCTGCCCC CTGGAGGGGA GGGTGGCCCT GAGGCCACTG 
     2401 TGGTGTCAGA CGAAGACGAG GCTGCTGAGA TGGAGGATGA GGCAGTGAGG AGCCGTGTGC 
     2461 AGCACAAGCC CAAGGAGGAC AAGCTCAGGC TAGCACAGGA GCTCTCTGAC ATGGTCATTT 
     2521 ACTGCAAGAG TGTCCACTTT GGGGGCTTCT CCAGTCCTGG CACCCCTGGA CAGGCCTTCT 
     2581 ACGAGATGGC GTCCTTCTCT GAGAACCGTG CCCTTCGACT GCTCCAAGAA TCAGGAAACG 
     2641 GCTTTGTCCG CCACAACGTG GGGCACCTGA GCAGAATCTA CCCGGCTGGA TGGAGAACAG 
     2701 ACTCCTCCAA CTACAGCCCC GTGGAGATGT GGAATGGGGG CTGCCAGATC GTGGCCCTGA 
     2761 ATTTCCAGAC ACCTGGGCCA GAGATGGACG TGTACCAGGG CCGCTTCCAG GACAACGGGG 
     2821 CCTGTGGGTA CGTGCTGAAG CCCGCCTTCC TGCGAGACCC CAACGGCACC TTTAACCCCC 
     2881 GCGCCCTGGC TCAGGGGCCC TGGTGGGCAC GGAAGCGGCT CAACATCAGG GTCATTTCGG 
     2941 GGCAGCAGCT GCCAAAAGTC AACAAGAATA AGAATTCAAT TGTGGACCCC AAAGTGACAG 
     3001 TGGAGATCCA TGGCGTGAGC CGGGACGTGG CCAGCCGCCA GACTGCTGTC ATCACCAACA 
     3061 ATGGTTTCAA CCCATGGTGG GACACGGAGT TTGCGTTTGA GGTAGTTGTG CCTGACCTTG 
     3121 CCCTCATCCG CTTCTTGGTG GAAGATTATG ATGCCTCCTC CAAGAATGAC TTCATTGGCC 
     3181 AGAGTACCAT CCCCTTGAAC AGCCTCAAGC AAGGATACCG CCATGTCCAC CTCATGTCTA 
     3241 AGAACGGGGA CCAGCATCCA TCAGCCACCC TCTTTGTGAA GATCTCCCTC CAGGACACGC 
     3301 GTACGCGGCC GCTCGAGATG GAGAGCGACG AGAGCGGCCT GCCCGCCATG GAGATCGAGT 
     3361 GCCGCATCAC CGGCACCCTG AACGGCGTGG AGTTCGAGCT GGTGGGCGGC GGAGAGGGCA 
     3421 CCCCCGAGCA GGGCCGCATG ACCAACAAGA TGAAGAGCAC CAAAGGCGCC CTGACCTTCA 
     3481 GCCCCTACCT GCTGAGCCAC GTGATGGGCT ACGGCTTCTA CCACTTCGGC ACCTACCCCA 
     3541 GCGGCTACGA GAACCCCTTC CTGCACGCCA TCAACAACGG CGGCTACACC AACACCCGCA 
     3601 TCGAGAAGTA CGAGGACGGC GGCGTGCTGC ACGTGAGCTT CAGCTACCGC TACGAGGCCG 
     3661 GCCGCGTGAT CGGCGACTTC AAGGTGATGG GCACCGGCTT CCCCGAGGAC AGCGTGATCT 
     3721 TCACCGACAA GATCATCCGC AGCAACGCCA CCGTGGAGCA CCTGCACCCC ATGGGCGATA 
     3781 ACGATCTGGA TGGCAGCTTC ACCCGCACCT TCAGCCTGCG CGACGGCGGC TACTACAGCT 
     3841 CCGTGGTGGA CAGCCACATG CACTTCAAGA GCGCCATCCA CCCCAGCATC CTGCAGAACG 
     3901 GGGGCCCCAT GTTCGCCTTC CGCCGCGTGG AGGAGGATCA CAGCAACACC GAGCTGGGCA 
     3961 TCGTGGAGTA CCAGCACGCC TTCAAGACCC CGGATGCAGA TGCCGGTGAA GAAAGAGTTT 
     4021 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4081 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4141 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4201 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4261 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4321 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4381 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4441 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4501 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4561 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4621 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4681 GAATTGGGTA CGCGGCCAGC TTGGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 
     4741 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG 
     4801 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 
     4861 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC 
     4921 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 
     4981 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 
     5041 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA 
     5101 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 
     5161 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 
     5221 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 
     5281 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 
     5341 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 
     5401 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 
     5461 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 
     5521 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 
     5581 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC 
     5641 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 
     5701 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCCGACCGC 
     5761 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 
     5821 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 
     5881 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA 
     5941 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT 
     6001 TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 
     6061 AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA 
     6121 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA 
     6181 AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC CTCTAGCTAG AGCTTGGCGT 
     6241 AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA 
     6301 TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT 
     6361 TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT 
     6421 AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT 
     6481 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 
     6541 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 
     6601 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 
     6661 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 
     6721 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 
     6781 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 
     6841 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 
     6901 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 
     6961 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 
     7021 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 
     7081 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 
     7141 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTTTT TTTGTTTGCA 
     7201 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 
     7261 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 
     7321 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 
     7381 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 
     7441 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 
     7501 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 
     7561 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 
     7621 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 
     7681 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 
     7741 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 
     7801 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 
     7861 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 
     7921 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 
     7981 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 
     8041 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 
     8101 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 
     8161 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 
     8221 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 
     8281 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8341 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8401 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8461 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8521 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     8581 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     8641 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     8701 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     8761 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     8821 CGAATTTT
//