LOCUS       RG207920                9584 bp    DNA     circular  SYN 
DEFINITION  HLTF (tGFP-tagged) - Human helicase-like transcription factor (HLTF), transcript variant 1
ACCESSION   NM_003071
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4065..4769 
                     /label="GFP"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    7493..8138 
                     /label="ColE1"  
     misc_feature    5855..6649 
                     /label="Neo"  
     misc_feature    5486..5788 
                     /label="SV40 ori"  
     misc_feature    8153..9013 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4044..4050 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4044 
                     /label="HLTF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTGGATG TTCAAGAGGG ATCCAGTTTG GAAGTACTTG CAGACTGTCC 
     1081 AGTATGGAGT TCATGGAAAT TTTCCACGCC TCTCATATCC AACTTTCTTT CCACGTTTTG 
     1141 AATTCCAAGA TGTTATCCCT CCAGATGACT TTCTAACTAG TGATGAAGAA GTAGATTCCG 
     1201 TTTTATTTGG AAGTTTGAGA GGTCATGTGG TTGGACTACG CTATTACACG GGAGTAGTTA 
     1261 ATAATAATGA AATGGTTGCA TTACAACGAG ATCCTAATAA CCCTTATGAT AAGAATGCAA 
     1321 TTAAAGTAAA CAATGTGAAT GGAAATCAAG TTGGCCATTT AAAGAAAGAG CTTGCAGGTG 
     1381 CTTTGGCCTA TATCATGGAC AACAAATTGG CACAAATTGA AGGGGTAGTT CCTTTTGGTG 
     1441 CAAACAATGC TTTTACCATG CCTCTGCATA TGACTTTTTG GGGAAAAGAA GAAAATAGAA 
     1501 AAGCGGTTTC AGATCAGTTG AAGAAACATG GATTTAAATT GGGTCCTGCA CCAAAAACTT 
     1561 TAGGATTCAA TTTGGAAAGT GGTTGGGGCT CTGGAAGAGC TGGACCAAGC TATAGTATGC 
     1621 CAGTGCATGC TGCAGTACAG ATGACAACTG AACAGCTTAA AACAGAATTT GACAAATTGT 
     1681 TTGAAGATTT AAAAGAAGAT GATAAAACCC ATGAAATGGA ACCAGCTGAG GCTATTGAAA 
     1741 CACCACTGCT TCCACATCAA AAACAAGCTC TAGCTTGGAT GGTGTCACGG GAAAATAGCA 
     1801 AAGAACTTCC ACCATTCTGG GAACAGCGAA ATGACTTATA CTATAACACA ATAACAAATT 
     1861 TTTCTGAGAA GGACCGACCA GAAAATGTCC ATGGAGGAAT TTTAGCTGAT GATATGGGTT 
     1921 TGGGTAAAAC TCTTACAGCC ATTGCAGTAA TCCTTACCAA CTTCCATGAT GGCAGACCTC 
     1981 TTCCTATTGA AAGAGTTAAA AAGAATCTAC TGAAGAAGGA ATATAATGTT AACGATGACT 
     2041 CTATGAAACT TGGAGGAAAC AATACCAGTG AAAAGGCAGA TGGACTAAGC AAAGACGCAT 
     2101 CTAGATGTAG TGAACAACCC AGTATTTCAG ATATCAAGGA GAAGAGTAAG TTTCGCATGT 
     2161 CAGAATTGTC TAGCTCCCGC CCCAAAAGAA GAAAAACTGC TGTCCAGTAC ATAGAAAGCA 
     2221 GTGATTCAGA GGAAATTGAA ACAAGTGAAT TGCCGCAGAA AATGAAAGGC AAACTGAAAA 
     2281 ATGTACAGTC TGAAACTAAA GGCAGGGCGA AAGGATCTTC TAAGGTTATA GAAGATGTGG 
     2341 CATTTGCATG TGCATTAACT TCATCTGTTC CTACAACAAA AAAGAAAATG TTGAAAAAGG 
     2401 GAGCTTGTGC AGTGGAGGGG TCAAAGAAAA CTGATGTTGA GGAGAGACCA AGAACAACAC 
     2461 TGATCATCTG TCCGCTTTCT GTGTTAAGCA ACTGGATTGA CCAGTTTGGA CAACATATAA 
     2521 AATCAGATGT ACACTTGAAT TTTTATGTTT ATTATGGTCC TGATCGTATT AGAGAACCGG 
     2581 CCTTACTTTC AAAACAGGAT ATTGTTTTGA CTACGTATAA TATTTTAACT CATGACTATG 
     2641 GAACTAAAGG AGATAGTCCA TTACATAGCA TAAGGTGGCT AAGAGTGATC CTGGATGAAG 
     2701 GACATGCCAT ACGAAATCCA AATGCTCAGC AGACAAAAGC TGTACTTGAC TTAGAATCAG 
     2761 AAAGAAGATG GGTTTTGACA GGTACTCCAA TCCAGAATTC TTTAAAGGAC TTGTGGTCTC 
     2821 TTCTTTCCTT TTTAAAACTT AAACCATTTA TTGATAGAGA ATGGTGGCAT AGAACAATAC 
     2881 AGCGTCCTGT CACAATGGGA GATGAAGGAG GACTTAGGCG TTTACAGTCC CTAATTAAAA 
     2941 ATATTACACT TAGAAGAACA AAGACAAGCA AAATTAAAGG AAAACCTGTT TTGGAGTTAC 
     3001 CAGAACGTAA AGTATTTATT CAGCACATTA CACTTTCAGA TGAAGAGAGA AAGATTTATC 
     3061 AGTCTGTGAA AAATGAAGGC AGAGCCACTA TTGGAAGGTA TTTTAATGAA GGGACTGTCC 
     3121 TGGCACATTA TGCAGATGTC CTGGGTCTTT TGCTTAGACT GCGGCAAATT TGTTGCCATA 
     3181 CTTACCTTCT TACAAATGCA GTGTCTTCCA ATGGCCCCTC AGGAAATGAT ACACCTGAAG 
     3241 AACTGAGAAA GAAGTTAATA AGGAAGATGA AGTTAATTCT GAGCTCAGGT TCAGATGAGG 
     3301 AATGTGCAAT TTGCCTGGAT TCTTTAACAG TTCCTGTGAT AACACATTGT GCACATGTAT 
     3361 TTTGTAAACC CTGTATTTGC CAAGTCATTC AGAATGAGCA GCCACATGCT AAATGCCCTT 
     3421 TATGCAGAAA TGATATACAT GAAGATAATT TATTAGAATG TCCTCCAGAA GAATTAGCAC 
     3481 GTGACAGTGA GAAAAAGTCT GATATGGAAT GGACATCCAG TTCAAAGATT AATGCGCTAA 
     3541 TGCACGCATT GACTGACTTA AGAAAGAAGA ATCCCAACAT AAAAAGTTTG GTTGTTTCTC 
     3601 AGTTTACAAC ATTCCTGTCT TTAATAGAAA TACCACTTAA AGCCTCTGGA TTTGTGTTTA 
     3661 CTCGTTTGGA TGGTTCCATG GCCCAAAAGA AAAGAGTTGA ATCAATTCAG TGTTTTCAAA 
     3721 ACACTGAAGC AGGATCTCCA ACTATAATGC TTCTGTCCTT AAAAGCAGGT GGAGTTGGTT 
     3781 TGAATCTGTC TGCAGCTTCT CGAGTGTTTT TAATGGATCC AGCCTGGAAT CCTGCTGCTG 
     3841 AAGATCAGTG CTTTGACAGA TGCCATAGAC TTGGTCAGAA GCAAGAAGTT ATCATCACAA 
     3901 AATTCATTGT AAAGGACTCT GTTGAAGAAA ATATGCTGAA AATACAAAAC AAAAAGAGAG 
     3961 AACTTGCAGC AGGAGCCTTT GGAACTAAAA AACCAAATGC TGACGAAATG AAACAAGCCA 
     4021 AAATTAATGA AATCAGAACA TTAATTGACT TAACGCGTAC GCGGCCGCTC GAGATGGAGA 
     4081 GCGACGAGAG CGGCCTGCCC GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG 
     4141 GCGTGGAGTT CGAGCTGGTG GGCGGCGGAG AGGGCACCCC CGAGCAGGGC CGCATGACCA 
     4201 ACAAGATGAA GAGCACCAAA GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA 
     4261 TGGGCTACGG CTTCTACCAC TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC 
     4321 ACGCCATCAA CAACGGCGGC TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG 
     4381 TGCTGCACGT GAGCTTCAGC TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG 
     4441 TGATGGGCAC CGGCTTCCCC GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA 
     4501 ACGCCACCGT GGAGCACCTG CACCCCATGG GCGATAACGA TCTGGATGGC AGCTTCACCC 
     4561 GCACCTTCAG CCTGCGCGAC GGCGGCTACT ACAGCTCCGT GGTGGACAGC CACATGCACT 
     4621 TCAAGAGCGC CATCCACCCC AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC 
     4681 GCGTGGAGGA GGATCACAGC AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA 
     4741 AGACCCCGGA TGCAGATGCC GGTGAAGAAA GAGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4801 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4861 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4921 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4981 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5041 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5101 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5161 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5221 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5281 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5341 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5401 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCTTGG 
     5461 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 
     5521 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA 
     5581 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 
     5641 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 
     5701 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG 
     5761 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA 
     5821 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT 
     5881 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA 
     5941 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG 
     6001 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG 
     6061 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT 
     6121 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT 
     6181 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG 
     6241 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT 
     6301 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC 
     6361 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC 
     6421 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA 
     6481 TTCATCGACT GTGGCCGGCT GGGTGTGGCC GACCGCTATC AGGACATAGC GTTGGCTACC 
     6541 CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT 
     6601 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA 
     6661 GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 
     6721 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 
     6781 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 
     6841 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 
     6901 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 
     6961 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 
     7021 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 
     7081 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 
     7141 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 
     7201 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 
     7261 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     7321 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     7381 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     7441 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     7501 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     7561 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     7621 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     7681 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     7741 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     7801 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     7861 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     7921 AACAAACCAC CGCTGGTAGC GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 
     7981 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 
     8041 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 
     8101 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 
     8161 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 
     8221 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 
     8281 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 
     8341 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 
     8401 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 
     8461 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 
     8521 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 
     8581 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 
     8641 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 
     8701 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 
     8761 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 
     8821 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 
     8881 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 
     8941 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 
     9001 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 
     9061 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 
     9121 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 
     9181 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 
     9241 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 
     9301 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 
     9361 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 
     9421 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 
     9481 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 
     9541 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//