LOCUS       RG207543                8936 bp    DNA     circular  SYN 
DEFINITION  HCFC2 (tGFP-tagged) - Human host cell factor C2 (HCFC2)
ACCESSION   NM_013320
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3417..4121 
                     /label="GFP"  
     misc_feature    4168..4753 
                     /label="PolyA signal"  
     misc_feature    6845..7490 
                     /label="ColE1"  
     misc_feature    5207..6001 
                     /label="Neo"  
     misc_feature    4838..5140 
                     /label="SV40 ori"  
     misc_feature    7505..8365 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3396..3402 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3396 
                     /label="HCFC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCTCCC AGCCTCCTCA ACTGGAGGCG AGTTTCTTCC TTCACGGGGC 
     1081 CGGTCCCCCG CGCCCGGCAC GGACACCGAG CGGTGGCCAT CCGGGAGCTG ATGATCATCT 
     1141 TTGGAGGGGG AAATGAGGGC ATCGCGGATG AGCTGCACGT CTACAACACG GCTACGAATC 
     1201 AGTGGTTTCT GCCAGCTGTT AGAGGAGATA TCCCTCCAGG CTGTGCTGCC CATGGATTTG 
     1261 TCTGTGATGG TACCAGAATA TTAGTATTTG GGGGAATGGT TGAATATGGA AGATACAGCA 
     1321 ATGAGTTATA TGAGTTACAA GCAAGTCGTT GGTTATGGAA AAAAGTGAAA CCCCATCCCC 
     1381 CTCCTTCTGG TTTACCTCCT TGTCCTCGGC TTGGACATAG CTTCTCTTTA TATGGTAACA 
     1441 AATGCTATTT GTTTGGTGGC CTGGCAAACG AAAGCGAAGA TTCAAACAAT AATGTTCCCA 
     1501 GATATTTAAA TGATTTTTAT GAGTTGGAGC TACAGCATGG CTCTGGTGTT GTGGGTTGGA 
     1561 GCATTCCAGT GACTAAAGGG GTTGTGCCTT CTCCAAGAGA ATCCCACACA GCTGTTATAT 
     1621 ATTGCAAAAA AGATTCTGGA AGTCCTAAAA TGTATGTTTT TGGTGGAATG TGTGGTGCTC 
     1681 GCCTGGATGA CCTATGGCAG CTTGACTTAG AAACTATGTC ATGGTCAAAA CCAGAAACTA 
     1741 AAGGGACAGT GCCACTTCCA CGAAGCCTTC ATACAGCCAG TGTTATAGGA AACAAGATGT 
     1801 ACATTTTTGG TGGATGGGTC CCACATAAGG GGGAAAATAC TGAGACTTCA CCTCATGATT 
     1861 GTGAATGGAG ATGTACCAGT TCATTTTCTT ACCTAAATCT GGATACAACA GAGTGGACCA 
     1921 CCCTAGTATC AGATTCTCAG GAAGATAAAA AAAATTCAAG ACCAAGACCA AGAGCTGGCC 
     1981 ACTGTGCTGT TGCAATCGGC ACTCGATTGT ATTTTTGGAG TGGAAGAGAT GGCTACAAAA 
     2041 AAGCACTGAA TAGTCAAGTT TGCTGCAAGG ATCTTTGGTA TCTTGATACT GAGAAACCAC 
     2101 CGGCACCATC TCAAGTACAG CTGATCAAAG CCACTACCAA CTCCTTTCAT GTCAAGTGGG 
     2161 ATGAAGTGTC TACAGTTGAG GGCTATCTTT TGCAGTTGAG TACAGACTTG CCATACCAAG 
     2221 CTGCATCATC AGATTCTTCA GCAGCACCAA ATATGCAAGG AGTCAGGATG GACCCTCACA 
     2281 GACAAGGCAG TAATAACATC GTTCCTAACA GTATCAATGA TACAATAAAC AGCACAAAAA 
     2341 CTGAACAGCC AGCCACAAAA GAAACTTCAA TGAAAAACAA ACCAGACTTT AAAGCACTGA 
     2401 CGGATTCTAA TGCCATTTTA TATCCATCTT TGGCATCAAA TGCTTCTAAT CATAATAGTC 
     2461 ATGTGGTGGA TATGCTAAGG AAAAATGAAG GTCCTCACAC TTCAGCAAAT GTAGGTGTTC 
     2521 TAAGTAGTTG CCTGGATGTA AGAACAGTAA TTCCTGAAAC ATCTGTATCC AGTACTGTTT 
     2581 CCAGCACACA AACTATGGTA ACCCAGCAGA CCATTAAAAC TGAATCATCC AGTACAAATG 
     2641 GGGCAGTTGT TAAAGATGAA ACTTCACTAA CAACATTCAG TACCAAATCT GAAGTTGATG 
     2701 AAACATATGC ACTGCCTGCA ACGAAGATCA GCCGTGTAGA GACACATGCT ACAGCAACGC 
     2761 CGTTTTCTAA AGAGACTCCT TCAAATCCAG TGGCCACAGT GAAAGCGGGA GAACGACAAT 
     2821 GGTGTGATGT GGGAATTTTT AAAAATAATA CAGCTTTGGT GAGCCAGTTT TATTTGCTGC 
     2881 CAAAAGGGAA GCAAAGCATC TCAAAGGTAG GAAATGCAGA TGTACCTGAC TACAGCTTGC 
     2941 TTAAGAAACA AGATCTTGTT CCAGGCACAG GATACAGATT CAGGGTTGCT GCAATCAATG 
     3001 GTTGTGGGAT AGGTCCTTTC AGCAAAATCA GTGAATTTAA AACTTGTATT CCTGGTTTTC 
     3061 CTGGAGCTCC TTCTGCAGTC AGAATTTCAA AGAATGTTGA AGGTATCCAC CTTTCCTGGG 
     3121 AACCTCCAAC CTCACCTTCT GGAAATATTT TGGAATATTC AGCCTACTTG GCTATCCGCA 
     3181 CAGCACAGAT ACAAGATAAT CCAAGTCAAC TTGTGTTCAT GAGGATTTAT TGTGGTCTTA 
     3241 AGACATCATG TATAGTAACT GCTGGGCAAC TTGCAAATGC ACATATTGAT TATACATCCA 
     3301 GGCCTGCCAT TGTGTTCAGG ATATCAGCAA AGAATGAAAA GGGATATGGA CCAGCTACAC 
     3361 AAGTTCGGTG GCTTCAAGGT AACAATAAGA AAGCACCTTT AAATACGCGT ACGCGGCCGC 
     3421 TCGAGATGGA GAGCGACGAG AGCGGCCTGC CCGCCATGGA GATCGAGTGC CGCATCACCG 
     3481 GCACCCTGAA CGGCGTGGAG TTCGAGCTGG TGGGCGGCGG AGAGGGCACC CCCGAGCAGG 
     3541 GCCGCATGAC CAACAAGATG AAGAGCACCA AAGGCGCCCT GACCTTCAGC CCCTACCTGC 
     3601 TGAGCCACGT GATGGGCTAC GGCTTCTACC ACTTCGGCAC CTACCCCAGC GGCTACGAGA 
     3661 ACCCCTTCCT GCACGCCATC AACAACGGCG GCTACACCAA CACCCGCATC GAGAAGTACG 
     3721 AGGACGGCGG CGTGCTGCAC GTGAGCTTCA GCTACCGCTA CGAGGCCGGC CGCGTGATCG 
     3781 GCGACTTCAA GGTGATGGGC ACCGGCTTCC CCGAGGACAG CGTGATCTTC ACCGACAAGA 
     3841 TCATCCGCAG CAACGCCACC GTGGAGCACC TGCACCCCAT GGGCGATAAC GATCTGGATG 
     3901 GCAGCTTCAC CCGCACCTTC AGCCTGCGCG ACGGCGGCTA CTACAGCTCC GTGGTGGACA 
     3961 GCCACATGCA CTTCAAGAGC GCCATCCACC CCAGCATCCT GCAGAACGGG GGCCCCATGT 
     4021 TCGCCTTCCG CCGCGTGGAG GAGGATCACA GCAACACCGA GCTGGGCATC GTGGAGTACC 
     4081 AGCACGCCTT CAAGACCCCG GATGCAGATG CCGGTGAAGA AAGAGTTTAA ACGGCCGGCC 
     4141 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4201 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4261 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4321 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4381 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4441 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4501 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4561 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4621 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4681 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4741 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4801 CGGCCAGCTT GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA 
     4861 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 
     4921 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 
     4981 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 
     5041 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG 
     5101 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 
     5161 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 
     5221 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 
     5281 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 
     5341 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 
     5401 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 
     5461 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 
     5521 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 
     5581 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 
     5641 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 
     5701 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 
     5761 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 
     5821 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CCGACCGCTA TCAGGACATA 
     5881 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 
     5941 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 
     6001 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 
     6061 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 
     6121 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 
     6181 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 
     6241 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 
     6301 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 
     6361 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 
     6421 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 
     6481 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 
     6541 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 
     6601 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 
     6661 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 
     6721 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 
     6781 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 
     6841 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 
     6901 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 
     6961 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 
     7021 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 
     7081 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 
     7141 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA 
     7201 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 
     7261 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA 
     7321 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 
     7381 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 
     7441 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 
     7501 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 
     7561 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 
     7621 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 
     7681 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 
     7741 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 
     7801 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 
     7861 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 
     7921 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 
     7981 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 
     8041 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 
     8101 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 
     8161 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 
     8221 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 
     8281 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 
     8341 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 
     8401 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 
     8461 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG 
     8521 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 
     8581 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 
     8641 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 
     8701 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 
     8761 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 
     8821 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA 
     8881 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//