LOCUS       RG207318                8567 bp    DNA     circular  SYN 
DEFINITION  MMP15 (tGFP-tagged) - Human matrix metallopeptidase 15 (membrane-inserted) (MMP15)
ACCESSION   NM_002428
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3048..3752 
                     /label="GFP"  
     misc_feature    3799..4384 
                     /label="PolyA signal"  
     misc_feature    6476..7121 
                     /label="ColE1"  
     misc_feature    4838..5632 
                     /label="Neo"  
     misc_feature    4469..4771 
                     /label="SV40 ori"  
     misc_feature    7136..7996 
                     /label="Amp"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3027..3033 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3027 
                     /label="MMP15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGCGAC CCGAGCGCGC CCGGACGGCC GGGCTGGACG GGCAGCCTCC 
     1081 TCGGCGACCG GGAGGAGGCG GCGCGGCCGC GACTGCTGCC GCTGCTCCTG GTGCTTCTGG 
     1141 GCTGCCTGGG CCTTGGCGTA GCGGCCGAAG ACGCGGAGGT CCATGCCGAG AACTGGCTGC 
     1201 GGCTTTATGG CTACCTGCCT CAGCCCAGCC GCCATATGTC CACCATGCGT TCCGCCCAGA 
     1261 TCTTGGCCTC GGCCCTTGCA GAGATGCAGC GCTTCTACGG GATCCCAGTC ACCGGTGTGC 
     1321 TCGACGAAGA GACCAAGGAG TGGATGAAGC GGCCCCGCTG TGGGGTGCCA GACCAGTTCG 
     1381 GGGTACGAGT GAAAGCCAAC CTGCGGCGGC GTCGGAAGCG CTACGCCCTC ACCGGGAGGA 
     1441 AGTGGAACAA CCACCATCTG ACCTTTAGCA TCCAGAACTA CACGGAGAAG TTGGGCTGGT 
     1501 ACCACTCGAT GGAGGCGGTG CGCAGGGCCT TCCGCGTGTG GGAGCAGGCC ACGCCCCTGG 
     1561 TCTTCCAGGA GGTGCCCTAT GAGGACATCC GGCTGCGGCG ACAGAAGGAG GCCGACATCA 
     1621 TGGTACTCTT TGCCTCTGGC TTCCACGGCG ACAGCTCGCC GTTTGATGGC ACCGGTGGCT 
     1681 TTCTGGCCCA CGCCTATTTC CCTGGCCCCG GCCTAGGCGG GGACACCCAT TTTGACGCAG 
     1741 ATGAGCCCTG GACCTTCTCC AGCACTGACC TGCATGGAAA CAACCTCTTC CTGGTGGCAG 
     1801 TGCATGAGCT GGGCCACGCG CTGGGGCTGG AGCACTCCAG CAACCCCAAT GCCATCATGG 
     1861 CACCGTTCTA CCAGTGGAAG GACGTTGACA ACTTCAAGCT GCCCGAGGAC GATCTCCGTG 
     1921 GCATCCAGCA GCTCTACGGT ACCCCAGACG GTCAGCCACA GCCTACCCAG CCTCTCCCCA 
     1981 CTGTGACGCC ACGGCGGCCA GGCCGGCCTG ACCACCGGCC GCCCCGGCCT CCCCAGCCAC 
     2041 CACCCCCAGG TGGGAAGCCA GAGCGGCCCC CAAAGCCGGG CCCTCCAGTC CAGCCCCGAG 
     2101 CCACAGAGCG GCCCGACCAG TATGGCCCCA ACATCTGCGA CGGGGACTTT GACACAGTGG 
     2161 CCATGCTTCG CGGGGAGATG TTCGTGTTCA AGGGCCGCTG GTTCTGGCGA GTCCGGCACA 
     2221 ACCGCGTCCT GGACAACTAT CCCATGCCCA TCGGGCACTT CTGGCGTGGT CTGCCCGGTG 
     2281 ACATCAGTGC TGCCTACGAG CGCCAAGACG GTCGTTTTGT CTTTTTCAAA GGTGACCGCT 
     2341 ACTGGCTCTT TCGAGAAGCG AACCTGGAGC CCGGCTACCC ACAGCCGCTG ACCAGCTATG 
     2401 GCCTGGGCAT CCCCTATGAC CGCATTGACA CGGCCATCTG GTGGGAGCCC ACAGGCCACA 
     2461 CCTTCTTCTT CCAAGAGGAC AGGTACTGGC GCTTCAACGA GGAGACACAG CGTGGAGACC 
     2521 CTGGGTACCC CAAGCCCATC AGTGTCTGGC AGGGGATCCC TGCCTCCCCT AAAGGGGCCT 
     2581 TCCTGAGCAA TGACGCAGCC TACACCTACT TCTACAAGGG CACCAAATAC TGGAAATTCG 
     2641 ACAATGAGCG CCTGCGGATG GAGCCCGGCT ACCCCAAGTC CATCCTGCGG GACTTCATGG 
     2701 GCTGCCAGGA GCACGTGGAG CCAGGCCCCC GATGGCCCGA CGTGGCCCGG CCGCCCTTCA 
     2761 ACCCCCACGG GGGTGCAGAG CCCGGGGCGG ACAGCGCAGA GGGCGACGTG GGGGATGGGG 
     2821 ATGGGGACTT TGGGGCCGGG GTCAACAAGG ACGGGGGCAG CCGCGTGGTG GTGCAGATGG 
     2881 AGGAGGTGGC ACGGACGGTG AACGTGGTGA TGGTGCTGGT GCCACTGCTG CTGCTGCTCT 
     2941 GCGTCCTGGG CCTCACCTAC GCGCTGGTGC AGATGCAGCG CAAGGGTGCG CCACGTGTCC 
     3001 TGCTTTACTG CAAGCGCTCG CTGCAGGAGT GGGTCACGCG TACGCGGCCG CTCGAGATGG 
     3061 AGAGCGACGA GAGCGGCCTG CCCGCCATGG AGATCGAGTG CCGCATCACC GGCACCCTGA 
     3121 ACGGCGTGGA GTTCGAGCTG GTGGGCGGCG GAGAGGGCAC CCCCGAGCAG GGCCGCATGA 
     3181 CCAACAAGAT GAAGAGCACC AAAGGCGCCC TGACCTTCAG CCCCTACCTG CTGAGCCACG 
     3241 TGATGGGCTA CGGCTTCTAC CACTTCGGCA CCTACCCCAG CGGCTACGAG AACCCCTTCC 
     3301 TGCACGCCAT CAACAACGGC GGCTACACCA ACACCCGCAT CGAGAAGTAC GAGGACGGCG 
     3361 GCGTGCTGCA CGTGAGCTTC AGCTACCGCT ACGAGGCCGG CCGCGTGATC GGCGACTTCA 
     3421 AGGTGATGGG CACCGGCTTC CCCGAGGACA GCGTGATCTT CACCGACAAG ATCATCCGCA 
     3481 GCAACGCCAC CGTGGAGCAC CTGCACCCCA TGGGCGATAA CGATCTGGAT GGCAGCTTCA 
     3541 CCCGCACCTT CAGCCTGCGC GACGGCGGCT ACTACAGCTC CGTGGTGGAC AGCCACATGC 
     3601 ACTTCAAGAG CGCCATCCAC CCCAGCATCC TGCAGAACGG GGGCCCCATG TTCGCCTTCC 
     3661 GCCGCGTGGA GGAGGATCAC AGCAACACCG AGCTGGGCAT CGTGGAGTAC CAGCACGCCT 
     3721 TCAAGACCCC GGATGCAGAT GCCGGTGAAG AAAGAGTTTA AACGGCCGGC CGCGGTCATA 
     3781 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3841 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3901 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3961 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4021 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4081 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4141 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4201 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4261 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4321 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4381 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCT 
     4441 TGGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 
     4501 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 
     4561 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 
     4621 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 
     4681 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 
     4741 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 
     4801 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 
     4861 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 
     4921 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 
     4981 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 
     5041 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 
     5101 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 
     5161 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 
     5221 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 
     5281 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 
     5341 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 
     5401 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 
     5461 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCCGACCGCT ATCAGGACAT AGCGTTGGCT 
     5521 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 
     5581 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 
     5641 TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 
     5701 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 
     5761 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 
     5821 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 
     5881 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 
     5941 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 
     6001 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 
     6061 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 
     6121 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 
     6181 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 
     6241 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 
     6301 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 
     6361 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 
     6421 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 
     6481 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 
     6541 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 
     6601 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 
     6661 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 
     6721 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 
     6781 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 
     6841 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 
     6901 GCAAACAAAC CACCGCTGGT AGCGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     6961 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     7021 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     7081 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 
     7141 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 
     7201 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 
     7261 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 
     7321 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 
     7381 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 
     7441 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 
     7501 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 
     7561 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 
     7621 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 
     7681 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 
     7741 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 
     7801 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 
     7861 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 
     7921 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 
     7981 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8041 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8101 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     8161 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     8221 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     8281 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     8341 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     8401 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     8461 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     8521 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//